Hichrom C8 and C18 色谱柱

• Capillary to preparative scale dimensions
• ISO 9001:2008 (Quality) and ISO 14001:2004 (Environmental) standards
• Every column exceeds stringent quality test criteria
• Exceptional reproducibility and efficiency
• See also Partisil/Partisphere and Ultrasphere sections

Hichrom has been firmly established in the field of chromatography for over 30 years, specialising in the development, manufacture and supply of high quality HPLC products, both in the UK and throughout the entire world. During this time Hichrom has built up a strong reputation amongst chromatographers for technical expertise, speed of delivery and competitive pricing. Hichrom is accredited to both ISO 9001:2008 (Quality) and ISO 14001:2004 (Environmental) standards. Every Hichrom manufactured column exceeds stringent quality criteria and all columns are supplied with documentation enabling a complete audit trail from the time of manufacture to the point of use. In addition to the following range of Hichrom branded columns (pages 132-140) Hichrom offer a wide range of HPLC columns packed with any commercially available silica (see page 63), an OEM manufacturing and custom bonding service and also manufacture the Partisil and Partisphere (see pages 189-193) and the Ultrasphere (see pages 258-259 ) brands.

Hichrom C8 and C18

Hichrom’s C8 and C18 columns offer high performance in order to tackle the most challenging reversed-phase applications. The use of ultra pure silica, advanced bonding technology, superior column specification and comprehensive batch validation all contribute to the columns’ excellent reproducibility.

Hichrom Phases

Phase	Functional Group	Endcapped	Particle Size (μm)	Pore Size (Å)	Surface Area (m2/g)	Carbon Load (%)
C18	Octadecyl	Yes	3.5, 5	150	250	15
C8	Octyl	Yes	3.5, 5	150	250	8

Excellent Batch Reproducibility

Combining ultra pure silica with advanced bonding technology results in a densely bonded silica that is both highly robust and highly reproducible. Unpredictable interactions between residual silanol sites on the silica surface and the analyte are effectively eliminated. Comprehensive batch validation ensures absolute batch to batch reproducibility is maintained with acidic, basic and neutral molecules. Figures 1 and 2 show two of the tests used during the validation of Hichrom C8 and C18 phases.

Test Conditions		Test Components		Test Conditions	Test Components
Column: Hichrom 5 C18 (250 x 4.6 mm)		1. Uracil		Column: Hichrom 5 C18 (250 x 4.6 mm)	1. Norephedrine
Eluent: CH3CN – 0.05M KH2PO4, pH 3.2 (60:40)		2.	Pyridine	Eluent: CH3OH – 0.025M KH2PO4, pH 6.0 (80:20)	2. Nortriptyline
Flow rate: 1 ml/min		3.	Phenol	Flow rate: 1 ml/min	3. Toluene
Temperature: Ambient	3	4.	Dimethyl phthalate	Temperature: Ambient	4. Imipramine
		5.	N,N-Dimethylaniline		5. Amitriptyline
				3
		6.	4-Butylbenzoic acid
		7. Toluene		2
2		5	7
1					4
				1	5
	4
		6

 

0	1	2	3	4	5	6	7	8	9   Mins	0	1	2	3	4	5	6	7	8	Mins
Figure 1. Validation test – Selectivity with acidic, basic and neutral molecules										Figure 2. Validation test – Tricyclic antidepressants

Every Hichrom C8 and C18 column is individually manufactured and meticulously tested in the Hichrom laboratory. Efficiency measurements and two peak asymmetry calculations are recorded for each component of the quality control test mixture. Samples of the quality control test mixture are available on request. Only columns exceeding the most stringent efficiency and peak shape specifications are approved for sale.

All Hichrom C8 and C18 columns are supplied with a Test Chromatogram and Batch Validation Certificate. Information regarding column care and use is displayed on the reverse of the Test Chromatogram.

e research and development.

Bonding Stability

Combining a unique dense bonding process (inhibiting ligand cleavage at low pH) with a revolutionary capping process (shielding the silica from dissolution at high pH) enables Hichrom C8 and C18 columns to offer exceptional stability across an extended pH range. Figure 5 demonstrates the acid robustness of a Hichrom 5 C18 column.

Column Robustness

A stable packed silica bed is critical to the long term performance of the column. Hichrom C8 and C18 columns are packed to extremely high efficiencies with excellent peak asymmetries, ensuring that high performance is maintained throughout the column’s lifetime. Figure 6 shows that the performance of both Hichrom 3.5μm C18 and Hichrom 5μm C18 columns remains unaffected after extended use.

C18 column dimensions, to further extend column lifetime by preventing irreversible adsorption and frit blockage at the top of the column. As shown in Figure 7, a fingertight column coupler (HI-081) is used to connect the guard holder (HI-161) to a Hichrom 5 C18 column. Further information on the guard cartridge range is listed on pages 24 and 25.

Hichrom

Ordering Information – Hichrom C8 and C18

Microbore (1.0mm i.d.) Columns

Hichrom Phase		Column Dimensions1 (mm)			Guard Cartridges	2
50 x 1.0	100 x 1.0	150 x 1.0	250 x 1.0	(For 1.0mm i.d. Columns)
3.5μm				–	(5/pk)3
C8	HI-3.5C8-50M	HI-3.5C8-100M	HI-3.5C8-150M	–	HI-3.5C8-10CE5
C18	HI-3.5C18-50M	HI-3.5C18-100M	HI-3.5C18-150M	–	HI-3.5C18-10CE5
5μm					(5/pk)3
C8	HI-5C8-50M	HI-5C8-100M	HI-5C8-150M	HI-5C8-250M	HI-5C8-10CE5
C18	HI-5C18-50M	HI-5C18-100M	HI-5C18-150M	HI-5C18-250M	HI-5C18-10CE5

Microbore (2.1mm i.d.) Columns

Hichrom Phase		Column Dimensions1 (mm)			Guard Cartridges	2
	50 x 2.1	100 x 2.1	150 x 2.1	250 x 2.1	(For 2.1mm i.d. Columns)
3.5μm				–	(5/pk)3
C8	HI-3.5C8-50AM	HI-3.5C8-100AM	HI-3.5C8-150AM	–	HI-3.5C8-10CM5
C18	HI-3.5C18-50AM	HI-3.5C18-100AM	HI-3.5C18-150AM	–	HI-3.5C18-10CM5
5μm					(5/pk)3
C8	HI-5C8-50AM	HI-5C8-100AM	HI-5C8-150AM	HI-5C8-250AM	HI-5C8-10CM5
C18	HI-5C18-50AM	HI-5C18-100AM	HI-5C18-150AM	HI-5C18-250AM	HI-5C18-10CM5

Medium Bore (3.2mm i.d.) Columns

Hichrom Phase		Column Dimensions1 (mm)			Guard Cartridges	2
	50 x 3.2	100 x 3.2	150 x 3.2	250 x 3.2	(For 3.2mm i.d. Columns)
3.5μm				–	(5/pk)3
C8	HI-3.5C8-50AS	HI-3.5C8-100AS	HI-3.5C8-150AS	–	HI-3.5C8-10C5
C18	HI-3.5C18-50AS	HI-3.5C18-100AS	HI-3.5C18-150AS	–	HI-3.5C18-10C5
5μm					(5/pk)3
C8	HI-5C8-50AS	HI-5C8-100AS	HI-5C8-150AS	HI-5C8-250AS	HI-5C8-10C5
C18	HI-5C18-50AS	HI-5C18-100AS	HI-5C18-150AS	HI-5C18-250AS	HI-5C18-10C5

Analytical (4.0mm i.d.) Columns

Hichrom Phase		Column Dimensions1 (mm)			Guard Cartridges	2
	50 x 4.0	100 x 4.0	150 x 4.0	250 x 4.0	(For 4.0mm i.d. Columns)
3.5μm				–	(5/pk)3
C8	HI-3.5C8-50AF	HI-3.5C8-100AF	HI-3.5C8-150AF	–	HI-3.5C8-10C5
C18	HI-3.5C18-50AF	HI-3.5C18-100AF	HI-3.5C18-150AF	–	HI-3.5C18-10C5
5μm					(5/pk)3
C8	HI-5C8-50AF	HI-5C8-100AF	HI-5C8-150AF	HI-5C8-250AF	HI-5C8-10C5
C18	HI-5C18-50AF	HI-5C18-100AF	HI-5C18-150AF	HI-5C18-250AF	HI-5C18-10C5

Analytical (4.6mm i.d.) Columns

Hichrom Phase		Column Dimensions1 (mm)			Guard Cartridges	2
	50 x 4.6	100 x 4.6	150 x 4.6	250 x 4.6	(For 4.6mm i.d. Columns)
3.5μm				–	(5/pk)3
C8	HI-3.5C8-50A	HI-3.5C8-100A	HI-3.5C8-150A	–	HI-3.5C8-10C5
C18	HI-3.5C18-50A	HI-3.5C18-100A	HI-3.5C18-150A	–	HI-3.5C18-10C5
5μm					(5/pk)3
C8	HI-5C8-50A	HI-5C8-100A	HI-5C8-150A	HI-5C8-250A	HI-5C8-10C5
C18	HI-5C18-50A	HI-5C18-100A	HI-5C18-150A	HI-5C18-250A	HI-5C18-10C5

Semi-Preparative (7.75mm i.d.) Columns

Hichrom Phase			Column Dimensions1 (mm)					Guard Cartridges		4
		50 x 7.75	100 x 7.75		150 x 7.75		250 x 7.75	(For 7.75mm i.d. Columns)
5μm									(3/pk)
C8		HI-5C8-50SP	HI-5C8-100SP		HI-5C8-150SP		HI-5C8-250SP		HI-5C8-10CP3
C18		HI-5C18-50SP	HI-5C18-100SP		HI-5C18-150SP		HI-5C18-250SP		HI-5C18-10CP3
Other column dimensions available – please enquire				3	Starter kits	available – see p. 25
Use with free-standing holder HI-161		and column coupler HI-081	– see p. 24	4	Use with free-standing holder HI-150		and column coupler HI-081		– see p. 24

Hichrom

Ordering Information – Hichrom C8 and C18 (continued)

Semi-Preparative (10.0mm i.d.) Columns

Hichrom Phase		Column Dimensions1 (mm)			Guard Cartridges2
	50 x 10.0	100 x 10.0	150 x 10.0	250 x 10.0	(For 10.0mm i.d. Columns)
5μm					(3/pk)
C8	HI-5C8-50SP1	HI-5C8-100SP1	HI-5C8-150SP1	HI-5C8-250SP1	HI-5C8-10CP3
C18	HI-5C18-50SP1	HI-5C18-100SP1	HI-5C18-150SP1	HI-5C18-250SP1	HI-5C18-10CP3

Preparative (21.2mm i.d.) Columns

Hichrom Phase		Column Dimensions1 (mm)			Guard Cartridges	2
	50 x 21.2	100 x 21.2	150 x 21.2	250 x 21.2	(For 21.2mm i.d. Columns)
5μm					(3/pk)
C8	HI-5C8-50P	HI-5C8-100P	HI-5C8-150P	HI-5C8-250P	HI-5C8-10CP3
C18	HI-5C18-50P	HI-5C18-100P	HI-5C18-150P	HI-5C18-250P	HI-5C18-10CP3

 

Preparative (30.0mm i.d.) Columns

	Hichrom Phase			Column Dimensions1 (mm)				Guard Cartridges	3
			50 x 30.0	100 x 30.0	150 x 30.0	250 x 30.0		(For 30.0mm i.d. Columns)
	5μm							(1/pk)
	C8		HI-5C8-50P3	HI-5C8-100P3	HI-5C8-150P3	HI-5C8-250P3		HI-5C8-20CP
	C18		HI-5C18-50P3	HI-5C18-100P3	HI-5C18-150P3	HI-5C18-250P3		HI-5C18-20CP
1	Other column dimensions available – please enquire			3 Use with free-standing holder HI-183		and column coupler HI-083
2	Use with free-standing holder HI-150		and column coupler HI-081