GE磁珠系列产品
GE磁珠产品包括Sera-Mag与Mag Sepharose两类,其中Sera-Mag为1μm超顺磁磁珠,可用于mRNA及核酸制备,以及蛋白的小规模纯化与制备;而Mag Sepharose则为高度交联的琼脂糖基架磁珠,一般用于磷酸化肽段富集,免疫沉淀,Pull-down,以及抗体及His标签蛋白的小规模制备与筛选。
Mag Sepharose ag Sepharose产品具有如下特点:
·操作简单快捷,仅需磁力架即可完成纯化;
·方法成熟可靠,结果重复性高,提高Pull-down、免疫沉淀实验的成功率;
·蛋白载量高,高达22 μg human IgG/ml填料(以Protein G配基为例);
·产品品种齐全,包含TiO2(纯化磷酸化肽段)、Protein A、Protein G、Ni螯合(His标签纯化)等多种配基。
Sera-Mag Sera-Mag磁珠的主要特点为:
·μm超顺磁磁珠,粒径均一;
·独特的菜花状表面提供了更*的结合能力;
·磁相应时间短,可快速分离,分离通量及精度高;
·*的胶体稳定性及单分散性;
·产品种类齐全,多种配基,如羧基、氨基、Protein A/G融合、亲和素、Oligo (dT)供选择;
·具有常速磁珠(Sera-Mag,单层磁层)及快速磁珠(SpeedBeads,双层磁层,更快的磁相应速度,有效缩短反应时间,提高精确度)可供选择。
应用 | 产品 | 货号 | 配基/表面 | 包装 | 备注 |
磷酸化肽段的捕获和富集 | TiO2 Mag Sepharose | 28944010 | TiO2 | 1×500 μl (20% medium slurry) | 提供5×500 μl包装 |
免疫沉淀、Pull-down、磷酸化蛋白及低丰度蛋白富集(间接法) | Protein A Mag Sepharose | 28944006 | Protein A | 1×500 μl (20% medium slurry) | 提供4×500 μl包装 |
Protein G Mag Sepharose | 28944008 | Protein G | 1×500 μl (20% medium slurry) | 提供4×500 μl包装 | |
Protein A/G HP SpinTrap Buffer Kit | 28913567 | 提供目标蛋白富集和免疫沉淀配套试剂 | 一套 | ||
Sera-Mag SpeedBeads Protein A/G | 17152104010150 | Protein A/G融合蛋白 | 5 ml (10 mg/ml) | 提供1 ml及100 ml包装 | |
免疫沉淀、Pull -down、磷酸化蛋白及低丰度蛋白富集(直接法) |
NHS Mag Sepharose | 28944009 | 预活化NHS集团 | 1×500 μl (20% medium slurry) | 提供4×500 μl包装 |
NHS HP SpinTrap Buffer Kit | 28913569 | 提供目标蛋白富集和免疫沉淀配套试剂 | 一套 | ||
Sera-Mag magnetic carboxylate | 44152105050250 | 羧基/Hydrophobic | 15 ml (50 mg/ml) | 提供100 ml及1 L包装 | |
Sera-Mag magnetic carboxylate | 24152105050250 | 羧基/Hydrophilic | 15 ml (50 mg/ml) | 提供100 ml及1 L包装 | |
Sera-Mag SpeedBeads carboxylate | 65152105050250 | 羧基/Hydrophobic | 15 ml (50 mg/ml) | 提供100 ml及1 L包装 | |
Sera-Mag SpeedBeads carboxylate | 45152105050250 | 羧基/Hydrophilic | 15 ml (50 mg/ml) | 提供100 ml及1 L包装 | |
抗体小规模纯化、克隆筛选 | Protein A Mag SepharoseXtra | 28967056 | Protein A | 2×1 ml (10% medium slurry) | 提供5×1 ml包装 |
Protein G Mag SepharoseXtra | 28967066 | Protein G | 2×1 ml (10% medium slurry) | 提供5×1 ml包装 | |
AB Buffer Kit | 28903059 | 提供抗体纯化配套试剂 | 一套 | ||
纯化更大量抗体及抗体片段,请参阅亲和层析填料章节 | |||||
His标签蛋白小规模纯化、筛选 | His Mag Sepharose Ni | 28967388 | Ni螯合 | 2×1 ml (5% medium slurry) | 提供5×1 ml及10×1 ml包装 |
His Mag Sepharose excel | 17371220 | Ni Sepharose excel | 2×1 ml (10% medium slurry) | 提供5×1 ml及10×1 ml包装 | |
His Buffer Kit | 11003400 | 提供His纯化配套试剂 | 一套 | ||
纯化更大量His标签片段,请参阅亲和层析填料章节 | |||||
生物素标签蛋白、核酸、多肽制备与富集 | Streptavidin Mag Sepharose | 28985738 | 链霉亲和素 | 2×1 ml (10% medium slurry) | 提供5×1 ml包装 |
Sera-Mag Streptavidin-Coated Magnetic Particles | 30152103010150 | 链霉亲和素(低生物素结合量,2500-3500 pmol/mg) | 5 ml (10 mg/ml) | 提供1 ml及100 ml包装 | |
Sera-Mag Streptavidin-Coated Magnetic Particles | 30152104010150 | 链霉亲和素(中等生物素结合量,3500-4500 pmol/mg) | 5 ml (10 mg/ml) | 提供1 ml及100 ml包装 | |
Sera-Mag Streptavidin-Coated Magnetic Particles | 30152105010150 | 链霉亲和素(高生物素结合量,4500-5500 pmol/mg) | 5 ml (10 mg/ml) | 提供1 ml及100 ml包装 | |
Sera-Mag SpeedBeads Streptavidin -Coated Magnetic Particles |
66152104010150 | 链霉亲和素(中等生物素结合量,3500-4500 pmol/mg) | 5 ml (10 mg/ml) | 提供1 ml及100 ml包装 | |
Sera-Mag SpeedBeads Streptavidin -Blocked Magnetic Particles |
21152104010150 | 链霉亲和素提供 | 5 ml (10 mg/ml) | 提供1 ml及100 ml包装 | |
Sera-Mag SpeedBeads Neutravavidin -Coated Magnetic Particles |
78152104010150 | 中性亲和素 | 5 ml (10 mg/ml) | 提供1 ml及100 ml包装 | |
纯化更大量生物素化蛋白或多肽,请参阅亲和层析填料章节 | |||||
mRNA提取与制备 | Sera-Mag Oligo(dT)-Coated Magnetic Particles |
38152103010 | Oligo(dT)14 | 5 ml (10 mg/ml) | 提供1 ml及100 ml包装 |
另外,GE还提供磁力架产品。MagRack 6磁力架,可并行处理6个1.5 ml离心管的磁珠纯化。离心管一旦放在磁力架上,磁珠和目标蛋白在几秒钟内便可集中于磁铁附近,在轻松去除上清同时确保蛋白得率。选购货号为:28-9489-64。
如果需要处理更大的样品体积,可以选择MagRack Maxi磁力架,可处理高达50 ml样品。选购货号为:28-9864-41。
产品名称 | 货号 | 用途 | 包装 | |
PD-10 重力柱配套 | PD-10 空柱 | 17043501 | 客户自行灌制亲和层析柱 | 50 个 |
LabMate PD-10 Buffer Reservoir | 18321603 | 缓冲液储槽,可增加PD-10缓冲液体积至25ml | 10 个 | |
PD-10 Spin Adapter | 28923245 | PD-10 在离心机使用配套 | 10 个 | |
MidiSpin Adapter | 28923244 | MidiTrap在离心机上使用配套 | 10个 | |
MiniSpin Adapter | 28923243 | MiniTrap在离心机上使用配套 | 10个 | |
MultiTrap 96孔板式配套 | Collection Plate | 28403943 | MultiTrap 和PreDictor 配套收集板,96-well plate 500 μl, V-shaped bottom | 5 ×96-well plates |
产品列表:
应用 | 产品 | 货号 | 配基/表面 | 包装 | 价格(美金) |
磷酸化肽段的捕获和富集 | TiO2 Mag Sepharose | 28-9440-10 | TiO2 | 1×500 μl(20% medium slurry) | 138 |
TiO2 Mag Sepharose | 28-9513-77 | TiO2 | 4×500 μl (20% medium slurry) | 517 | |
免疫沉淀、 Pull-down、磷酸化蛋白及低丰度蛋白富集 (间接法) |
Protein A Mag Sepharose | 28-9440-06 | Protein A | 1×500 μl(20% medium slurry) | 208 |
Protein A Mag Sepharose | 28-9513-78 | Protein A | 4×500 μl (20% medium slurry) | 801 | |
Protein G Mag Sepharose | 28-9440-08 | Protein G | 1×500 μl(20% medium slurry) | 206 | |
Protein G Mag Sepharose | 28-9513-79 | Protein G | 4×500 μl (20% medium slurry) | 801 | |
Protein A/G HP SpinTrap Buffer Kit | 28-9135-67 | 提供目标蛋白富集和免疫沉淀配套试剂 | 一套 | 338 | |
Sera-Mag SpeedBead Protein A/G | 17152104011150 | Protein A/G 混合模式 | 1ml(10mg/ml) | 184 | |
Sera-Mag SpeedBead Protein A/G | 17152104010150 | Protein A/G 混合模式 | 5ml(10mg/ml) | 612 | |
Sera-Mag SpeedBead Protein A/G | 17152104010350 | Protein A/G 混合模式 | 100ml(10mg/ml) | 询价 | |
免疫沉淀、 Pull-down、磷酸化蛋白及低丰度蛋白富集 (直接法) |
NHS Mag Sepharose | 28-9440-09 | 预活化NHS 基团 | 1×500 μl(20% medium slurry) | 206 |
NHS Mag Sepharose | 28-9513-80 | 预活化NHS 基团 | 4×500 μl (20% medium slurry) | 801 | |
NHS HP SpinTrap Buffer Kit | 28-9135-69 | 提供目标蛋白富集和免疫沉淀配套试剂 | 一套 | 390 | |
Sera-Mag magnetic carboxylate | 44152105050250 | 羧基/Hydrophobic | 15ml(50mg/ml) | 321 | |
Sera-Mag magnetic carboxylate | 44152105050350 | 羧基/Hydrophobic | 100ml(50mg/ml) | 询价 | |
Sera-Mag magnetic carboxylate | 44152105050450 | 羧基/Hydrophobic | 1000ml(50mg/ml) | 询价 | |
Sera-Mag magnetic carboxylate | 24152105050250 | 羧基/Hydrophobic | 15ml(50mg/ml) | 321 | |
Sera-Mag magnetic carboxylate | 24152105050350 | 羧基/Hydrophobic | 100ml(50mg/ml) | 询价 | |
Sera-Mag magnetic carboxylate | 24152105050450 | 羧基/Hydrophobic | 1000ml(50mg/ml) | 询价 | |
Sera-Mag SpeedBead carboxylate | 65152105050250 | 羧基/Hydrophobic | 15ml(50mg/ml) | 450 | |
Sera-Mag SpeedBead carboxylate | 65152105050350 | 羧基/Hydrophobic | 100ml(50mg/ml) | 询价 | |
Sera-Mag SpeedBead carboxylate | 65152105050450 | 羧基/Hydrophobic | 1000ml(50mg/ml) | 询价 | |
Sera-Mag SpeedBead carboxylate | 45152105050250 | 羧基/Hydrophobic | 15ml(50mg/ml) | 450 | |
Sera-Mag SpeedBead carboxylate | 45152105050350 | 羧基/Hydrophobic | 100ml(50mg/ml) | 询价 | |
Sera-Mag SpeedBead carboxylate | 45152105050450 | 羧基/Hydrophobic | 1000ml(50mg/ml) | 询价 | |
SpeedBeads™具有两层磁层,在磁场中的响应速度是Sera-Mag磁珠的2倍,可用于从悬浮液中更快、更完整的分离目标分子 |
应用 | 产品 | 货号 | 配基/表面 | 包装 | 价格(美金) |
抗体小规模纯化、克隆筛选 | Protein A Mag Sepharose Xtra | 28-9670-56 | Protein A | 2×1 ml (10% medium slurry) | 255 |
Protein A Mag Sepharose Xtra | 28-9670-62 | Protein A | 5×1 ml (10% medium slurry) | 532 | |
Protein G Mag Sepharose Xtra | 28-9670-66 | Protein G | 2×1 ml (10% medium slurry) | 255 | |
Protein G Mag Sepharose Xtra | 28-9670-70 | Protein G | 5×1 ml (10% medium slurry) | 532 | |
Ab Buffer Kit | 28-9030-59 | 提供抗体纯化配套试剂 | 一套 | 158 | |
His 标签蛋白小规模纯化、筛选 | His Mag Sepharose Ni | 28-9673-88 | Ni螯合 | 2×1 ml (5% medium slurry) | 146 |
His Mag Sepharose Ni | 28-9673-90 | Ni螯合 | 5×1 ml (5% medium slurry) | 324 | |
His Mag Sepharose Ni | 28-9799-17 | Ni螯合 | 10×1 ml (5% medium slurry) | 590 | |
His Mag Sepharose excel | 17-3712-20 | Ni Sepharose excel | 2×1 ml (10% medium slurry) | 222 | |
His Mag Sepharose excel | 17-3712-21 | Ni Sepharose excel | 5×1 ml (10% medium slurry) | 456 | |
His Mag Sepharose excel | 17-3712-22 | Ni Sepharose excel | 10×1 ml (10% medium slurry) | 734 | |
His Buffer Kit | 11-0034-00 | 提供His纯化配套试剂 | 一套 | 155 | |
生物素标签蛋白、核酸、多 肽制备与富集 |
Streptavidin Mag Sepharose | 28-9857-38 | 链霉亲和素 | 2×1 ml (10% medium slurry) | 255 |
Streptavidin Mag Sepharose | 28-9857-99 | 链霉亲和素 | 5×1 ml (10% medium slurry) | 537 | |
Sera-Mag Streptavidin-Coated Magnetic | 30152103011150 | 链霉亲和素 (low binding capacity) | 1ml(10mg/ml) | 167 | |
Sera-Mag Streptavidin-Coated Magnetic | 30152103010150 | 链霉亲和素 (low binding capacity) | 5ml(10mg/ml) | 668 | |
Sera-Mag Streptavidin-Coated Magnetic | 30152104011150 | 链霉亲和素 (medium binding capacity) | 1ml(10mg/ml) | 193 | |
Sera-Mag Streptavidin-Coated Magnetic | 30152104010150 | 链霉亲和素 (medium binding capacity) | 5ml(10mg/ml) | 771 | |
Sera-Mag Streptavidin-Coated Magnetic | 30152105011150 | 链霉亲和素 (high binding capacity) | 1ml(10mg/ml) | 218 | |
Sera-Mag Streptavidin-Coated Magnetic | 30152105010150 | 链霉亲和素 (high binding capacity) | 5ml(10mg/ml) | 900 | |
Sera-Mag SpeedBeads Streptavidin-Coated Magnetic | 66152104011150 | 链霉亲和素 (medium binding capacity) | 1ml(10mg/ml) | 321 | |
Sera-Mag SpeedBeads Streptavidin-Coated Magnetic | 66152104010150 | 链霉亲和素 (medium binding capacity) | 5ml(10mg/ml) | 1028 | |
Sera-Mag SpeedBeads Streptavidin-Blocked Magnetic | 21152104011150 | 链霉亲和素 (low nonspecific binding) | 1ml(10mg/ml) | 450 | |
Sera-Mag SpeedBeads Streptavidin-Blocked Magnetic | 21152104010150 | 链霉亲和素 (low nonspecific binding) | 5ml(10mg/ml) | 1028 | |
Sera-Mag SpeedBeads Streptavidin-Blocked Magnetic | 21152104010350 | 链霉亲和素 (low nonspecific binding) | 100ml(10mg/ml) | 询价 | |
Sera-Mag SpeedBeads Neutravavidin-Coated Magnetic | 78152104011150 | 中性亲和素 | 1ml(10mg/ml) | 321 | |
Sera-Mag SpeedBeads Neutravavidin-Coated Magnetic | 78152104010150 | 中性亲和素 | 5ml(10mg/ml) | 1028 | |
mRNA提取与制备 | Sera-Mag Oligo(dT)-Coated Magnetic | 38152103011150 | Oligo(dT)14 | 1ml(10mg/ml) | 161 |
Sera-Mag Oligo(dT)-Coated Magnetic | 38152103010150 | Oligo(dT)14 | 5ml(10mg/ml) | 643 | |
Sera-Mag Oligo(dT)-Coated Magnetic | 38152103010350 | Oligo(dT)14 | 100ml(10mg/ml) | 询价 |