NISSUI日水RPMI 1640 Medium
Directions
Dissolve 10.2 g of the medium in distilled water and adjust the volume to 1,000mL. Sterilize by autoclaving at 121°C for 15 minutes. Cool the sterilized medium to room temperature and add a proper amount of sterile 10% sodium bicarbonate. (pH will be 7.1-7.4 at 37°C, in an atmosphere of 5% CO2 in air by adding 18-30 mL of sodium bicarbonate solution.) Aseptically add 0.3 g Lglutamine and the desire amount or serum.
Summary
RPMI 1640 is a medium for suspension culture investigated by George E. Moore, former director of Roswell Park Memorial Institute. Up to now, such media were not autoclavable because of decomposition or deterioration of the constituents, so that sterilization had been performed by filtration using a membrane filter. RPMI 1640 Medium “Nissui” 2 is an autoclavablepowdered medium specially manufactured to overcome the difficulties associated with filtersterilized media, such as contamination by Mycoplasma. This medium has growth promoting effect to primary cultures of mouse and human leukemia cells, various kinds of cell lines and KATO-III cells.
Storage and Expiration
Close tightly and store at 2-5°C in a dark and dry place. May be stored up to one (1) year.
Precautions
Do not use the product after its expiry date.
Quality of the product is not warranted after its shelf life.
When if medium or reagent touched eyes or mouth, immediately wash with plenty of water, and consult a physician. Any medium, reagent and materials must be sterilized by autoclaving or boiling water after use, and then disposed as industrial waste according to the law or regulations related to dispose.
Formula
|
Components
|
In 10.2 g/L
|
|
L-Arginine Hydrochloride
|
240.0 mg
|
|
L-Asparagine, H2O
|
56.8 mg
|
|
L-Asparatic Acid
|
20.0 mg
|
|
L-Cysteine Hydrochloride, H2O
|
72.9 mg
|
|
L-Glutamic Acid
|
20.0 mg
|
|
Succinic Acid
|
46.0 mg
|
|
Sodium Succinate,6 H2O
|
164.0 mg
|
|
Glutathione
|
1.0 mg
|
|
Glycine
|
10.0 mg
|
|
L-Histidine Hydrochloride, H2O
|
20.3 mg
|
|
L-Hydroxyproline
|
20.0 mg
|
|
L-Isoleucine
|
50.0 mg
|
|
L-Leucine
|
50.0 mg
|
|
L-Lysine Hydrochloride
|
40.0 mg
|
|
L-Methionine
|
15.0 mg
|
|
L-Phenylalanine
|
15.0 mg
|
|
L-Proline
|
20.0 mg
|
|
L-Serine
|
30.0 mg
|
|
L-Threonine
|
20.0 mg
|
|
L-Tryptophan
|
5.0 mg
|
|
L-Tyrosine
|
20.0 mg
|
|
L-Valine
|
20.0 mg
|
|
Biotin
|
0.2 mg
|
|
Calcium Pantothenate
|
0.25 mg
|
|
Choline Chloride
|
3.0 mg
|
|
Folic Acid
|
1.0 mg
|
|
Inositol
|
35.0 mg
|
|
Nicotinamide
|
1.0 mg
|
|
p-Aminobenzoic Acid
|
1.0 mg
|
|
Pyridoxine Hydrochloride
|
1.0 mg
|
|
Riboflavin
|
0.2 mg
|
|
Thiamin Hydrochloride
|
1.0 mg
|
|
Cyanocobalamin
|
0.005 mg
|
|
Sodium Chloride
|
6,000.0 mg
|
|
Potassium Chloride
|
400.0 mg
|
|
Calcium Nitrate
|
69.5 mg
|
|
Monosodium Phosphate
|
677.0 mg
|
|
Magnesium Sulfate
|
48.8 mg
|
|
Glucose
|
2,000.0 mg
|
|
Phenol Red
|
5.0 mg
|
00883 NISSUI 日本日水 RPMI 1640液体培养基RPMI 1640 Liquid
产品编号：00883
中文名称：RPMI 1640液体培养基
英文品名：RPMI 1640 Liquid
包装规格：500ml
用途：液态组织培养基
使用方法:
RPMI 1640 Liquid“日水”是根据由RPMI（Roswell Park Memorial Institute）的Director即Dr.George E.Moore开发出来的浮游细菌培养基制造而成的液态培养基。本培养基被用作老鼠和人的白血病细胞的第一代培养基以及株的第二代培养基。
在使用前，请根据不同目的添加适量血清。
组成（mg/L):
RPMI 1640液体培养基RPMI 1640 Liquid编号：00883
硝酸钙（四水盐）……………100
氯化钾…………………………400
硫酸镁（无水）………………48.84
氯化钠…………………………6000
磷酸一氢二钠（无水）………800
L-精氨酸盐酸盐………………200
L-天门冬酰胺…………………50
L-天冬氨酸……………………20
RPMI 1640液体培养基RPMI 1640 Liquid编号：00883
L-胱氨酸二盐酸盐……………65.15
L-谷氨酸………………………20
L-谷氨酰胺……………………300
甘氨酸…………………………10
L-组氨酸………………………15
L-羟脯氨酸……………………20
L-异亮氨酸……………………50
L-赖氨酸盐酸盐………………40
L-甲硫氨酸……………………15
L-苯基丙氨酸…………………15
L-脯氨酸………………………20
L-丝氨酸………………………30
L-苏氨酸………………………20
L-色氨酸………………………5
L-酷氨酸二钠（二水盐）……28.83
L-缬氨酸………………………20
生物素…………………………0.2
泛酸钙…………………………0.25
氯化胆碱………………………3
RPMI 1640液体培养基RPMI 1640 Liquid编号：00883
叶酸……………………………1
肌醇……………………………35
烟酰胺…………………………1
盐酸吡哆醛……………………1
核黄酸………………………0.2
盐酸硫胺………………………1
维他命B12……………………0.005
D．葡萄糖……………………2000
对氨基苯甲酸…………………1
谷胱甘肽（还原型）…………1
酚红（钠盐）………………5.3
碳酸氢钠……………………2000
贮藏方法、使用期限:
保存在低温（2~8℃）之处。使用期限为一年。