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Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。
Vector® Blue Substrate Kit, Alkaline Phosphatase (AP)
Description
The Vector Blue AP Substrate Kit produces a blue, heat stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.
Features:
- Greater sensitivity than conventional substrates
- Consistent and reliable
- Ideal for IHC/ICC/ISH and blots
- Fluorescent
- Heat Stable
- Permanent mounting
- Stock solutions supplied in convenient dropper bottles promoting ease of handling
- No wait times for mixing and dissolving powders or tablets
- Sufficient reagents to produce 200 ml of working solution
- Excitation: broad, peak at ~500 nm*
- Emission: broad, peak at ~680 nm*
*Histochem J. (1998) 8:577
Specifications
| Unit Size | 1 kit |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot |
| Detection Enzyme(s) | Alkaline Phosphatase |
| Substrate Reaction Product | Precipitating Reaction Product |
| Chromogen Color | Blue |
| Color of Fluorescence | Far Red |
| Microscopy | Brightfield, Fluorescence, Spectral Imaging |
| Additional Substrate Properties | Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling |
| Mounting | Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent) |
| Safety Title | WARNING: Reproductive Harm – www.P65Warnings.ca.gov |
Kit Components
Kit Contents:
- 4 ml Vector Blue Reagent 1
- 4 ml Vector Blue Reagent 2
- 3 ml Vector Blue Reagent 3
Documents
- Enzyme Substrates Brochure
- Enzyme Substrate Combinations Chart
- Counterstain/Substrate Compatibility Chart
- User Guide
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
BLOXALL® Endogenous Blocking Solution, Peroxidase and Alkaline Phosphatase Catalog #: SP-6000-100
Nuclear Fast Red Counterstain Catalog #: H-3403-500
Methyl Green Counterstain Catalog #: H-3402-500
VectaMount® Permanent Mounting Medium Catalog #: H-5000-60
VectaMount® AQ Aqueous Mounting Medium Catalog #: H-5501-60
Levamisole Solution Catalog #: SP-5000-18
Citations
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Vector Blue is both chromogenic and fluorescent. Sections stained with the Vector Blue substrate can be dehydrated, cleared, and permanently mounted; however, non-xylene clearing agents and non-xylene based mounting media (such as VectaMount®, H-5000) must be used because the reaction product is soluble in xylene. Vector Blue can also be used on blots in applications such as ELISPOT, singly or in combination with a peroxidase substrate such as AEC. This kit contains stock solutions in convenient dropper bottles.
