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Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。
VECTASHIELD® PLUS Antifade Mounting Medium with DAPI
Description
VECTASHIELD PLUS Antifade Mounting Medium with DAPI is a unique, stable formula for preserving fluorescence. This medium inhibits rapid photobleaching of fluorescent proteins and fluorescent dyes.
Features:
- No inherent toning or background
- Superior signal retention across the spectrum from blue to far red
- Mounted sections can be viewed in 1 hour
- DAPI (blue) counterstain premixed with medium
- Available in two convenient sizes
- Superior non-setting formulation
VECTASHIELD PLUS Mounting Medium with DAPI does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 2-8 °C, protected from light.
Specifications
| Unit Size | 2 ml, 10 ml |
|---|---|
| Applications | Immunofluorescence, In situ hybridization |
| Mounting | Aqueous (Non-Hardening) |
| Antifade | Yes |
| Counterstain | DAPI (blue fluorescence) |
Documents
- User Guide
- Safety Data Sheet
- VECTASHIELD Fluorochrome Compatibility
- Download CoA
- Datasheet
Related Products
VECTASHIELD® PLUS Antifade Mounting Medium Catalog #: H-1900
VECTASHIELD® Vibrance™ Antifade Mounting Medium with DAPI Catalog #: H-1800
VECTASHIELD® Vibrance™ Antifade Mounting Medium Catalog #: H-1700
VECTASHIELD® Antifade Mounting Medium Catalog #: H-1000-10
VECTASHIELD® Antifade Mounting Medium with DAPI Catalog #: H-1200-10
VECTASHIELD® Antifade Mounting Medium with Propidium Iodide (PI) Catalog #: H-1300-10
VECTASHIELD® HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10
Technical Information
The refractive index for VECTASHIELD Mounting Medium is 1.45.
VECTASHIELD PLUS Mounting Medium does not solidify, but rather, remains a liquid on the slide ‐ which can then be stored without sealing. If desired, coverslips can be sealed around the perimeter using nail polish or plastic sealant. Mounted slides should be stored at 2-8 °C and protected from light.
VECTASHIELD Mounting Medium Antifade Comparison
Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD mountants using frozen tissue sections that have been immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is then measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer's published results.
VECTASHIELD PLUS References:
1. González-Rodríguez, P. et al. (2020) The DNA methyltransferase DNMT3A contributes to autophagy long-term memory. Autophagy. (https://www.tandfonline.com/doi/full/10.1080/15548627.2020.1816664)
2. Viena, T. et al. (2020) Calretinin and calbindin architecture of the midline thalamus associated with prefrontal–hippocampal circuitry. Hippocampus (https://onlinelibrary.wiley.com/doi/abs/10.1002/hipo.23271)
3. Molina-García, L. et al. (2020) Direct glia-to-neuron transdifferentiation gives rise to a pair of male-specific neurons that ensure nimble male mating. eLife (https://elifesciences.org/articles/48361)
4. Valle, L. et al. (2021) Induction of Brain Tumors by the Archetype Strain of Human Neurotropic JCPyV in a Transgenic Mouse Model. Viruses (https://www.mdpi.com/1999-4915/13/2/162/htm)
