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Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。
Horse Anti-Mouse IgG Antibody (H+L), DyLight® 594
Description
Features:
- Affinity-purified, ultrapure, high affinity antibody
- Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
- Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L)
- Optimally labeled with DyLight® 594 to provide the brightest label for fluorescence microscopy
- Supplied in solution
- Excitation: 592 nm
- Emission: 617 nm
- Color: Red
Specifications
| Unit Size | 1.5 mg |
|---|---|
| Applications | Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation |
| Concentration | 1.5 mg active conjugate/ml |
| Recommended Storage | 2-8 °C |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. |
| Maximum Emission | 617 nm |
| Maximum Excitation | 592 nm |
| Recommended Usage | Recommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue. |
| Target Species | Mouse |
| Conjugate | DyLight 594 |
| Color of Fluorescence | Red |
| Host Species | Horse |
| Format | Concentrate |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
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Citations
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The anti-mouse Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. Unless otherwise specified, our antibodies will recognize both heavy and light chains (H+L).
DyLight® fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.
DyLight® dyes offer a number of potential advantages including greater photostability and brighter fluorescence. DyLight® dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight® dyes can be applied as single labels or in combination with other DyLight® dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology. The Dylight® dyes currently offered are DyLight® 488 (green), DyLight® 549 (orange), DyLight® 594 (red), and DyLight® 649 (far red).
