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Ricinus Communis Agglutinin I (RCA I, RCA120), Biotinylated
Description
This lectin consists of two subunits of 60 kDa which can be dissociated by reducing agents into closely related chains between 27 kDa and 33 kDa. One of the chains appears to be common to the “B” chain of another castor bean lectin, ricin, while the other chain is unique to RCA I. The B chain binds to galactose or N-acetylgalactosamine residues of membrane glycoconjugates.
Biotinylated RCA I has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
Specifications
| Unit Size | 5 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs |
| Recommended Usage | For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer. |
| Recommended Storage | 2-8 °C; DO NOT FREEZE |
| Inhibiting and/or Eluting Sugar | Inhibiting/Eluting Sugar: 200 mM galactose (S-9003) or lactose (S-9004). |
| Solution | 10 mM sodium phosphate, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 5 mM lactose |
| Concentration | 5 mg active conjugate/ml |
| Conjugate | Biotinylated |
| Sugar Specificity | Galactose, Lactose |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
Related Products
Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins Catalog #: ES-2100-100
Galactose Catalog #: S-9003
Lactose Catalog #: S-9004
Ricinus Communis Agglutinin I (RCA I, RCA120), Unconjugated Catalog #: L-1080-10
Ricinus Communis Agglutinin I (RCA I, RCA120), Fluorescein Catalog #: FL-1081-5
Ricinus Communis Agglutinin I (RCA I, RCA120), Rhodamine Catalog #: RL-1082-5
Citations
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Inhibiting/Eluting Sugar: 200 mM galactose or lactose