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Maackia Amurensis Lectin II (MAL II), Biotinylated
Description
Although the specificity of this lectin is not well defined, MAL II appears to bind only particular carbohydrate structures that contain sialic acid. Unlike Sambucus nigra lectin (SNA) which seems to prefer structures with (α-2,6) linked sialic acid, MAL II appears to bind sialic acid in an (α-2,3) linkage. Tissue staining patterns are also very different among MAL I, SNA and MAL II.
Biotinylated Maackia amurensis lectin II has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
Specifications
| Unit Size | 1 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
| Recommended Usage | For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer. |
| Recommended Storage | 2-8 °C |
| Solution | 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide. |
| Concentration | 1 mg active conjugate/ml |
| Conjugate | Biotinylated |
| Sugar Specificity | Sialic Acid |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
Related Products
Maackia Amurensis Lectin II (MAL II), Unconjugated Catalog #: L-1260-2
Sambucus Nigra Lectin (SNA, EBL), Unconjugated Catalog #: L-1300-5
Maackia Amurensis Lectin I (MAL I), Unconjugated Catalog #: L-1310-5
Lectin Kit I, Biotinylated Catalog #: BK-1000
Lectin Kit III, Biotinylated Catalog #: BK-3000
Streptavidin, DyLight® 594 Catalog #: SA-5594-1
Streptavidin, DyLight® 488 Catalog #: SA-5488-1
Citations
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This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Geisler, C and Jarvis, D.L. Glycobiology, vol. 21, no. 8 pp. 988-993, 2011
Inhibitor: Human glycophorin