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Protein A, Biotinylated (S. Aureus, Cowan strain)
Description
Biotinylated Protein A (B-2001), isolated from S. aureus, is a 41 kDa protein which binds to the Fc portion of most immunoglobulins. This interaction is neither species specific nor isotype specific, as Protein A has been shown to bind IgG from a variety of species. Protein A can also interact with IgA, IgM and subclasses of IgG from different species.
Specifications
| Unit Size | 1 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs |
| Recommended Usage | Reconstitute by adding 0.5 ml water.The resulting solution will have the following compostition:0.15 M NaCl, 10 mM HEPES, pH 8.0 The recommended concentration range for use is 2-5 µg/ml. For long term storage, an immunohistochemical grade, globulin-free bovine serum albumin (0.1%, w/v) may be added to the biotinylated Protein A solution prior to freezing.For short-term usage, bovine serum albumin may also be added and samples stored at 4 ºC in the presence of 0.05% sodium azide. |
| Recommended Storage | 2-8 °C after reconstitution; -20 °C is recommended. |
| Conjugate | Biotinylated |
| Format | Concentrate |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
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Citations
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Biotinylated Protein A is used in many systems as a substitute for a biotinylated second antibody. However, in some applications, Biotinylated Protein A may bind to endogenous immunoglobulin in the tissue producing background. In addition, Biotinylated Protein A may not bind to the species or class of primary antibody being employed. For routine immunohistochemical staining we recommend using biotinylated anti-immunoglobulins.