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VECTASHIELD® PLUS Antifade Mounting Medium
Description
VECTASHIELD PLUS Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD PLUS Mounting Medium inhibits rapid photobleaching of fluorescent proteins and fluorescent dyes.
Features:
- Ideal refractive index
- Ready to use
- No warming necessary
- Can be stored without sealing for long term analysis
- Easy to use
The original VECTASHIELD PLUS Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 2-8 °C, protected from light.
Specifications
| Unit Size | 2 ml, 10 ml |
|---|---|
| Applications | Immunofluorescence, In situ hybridization |
| Recommended Storage | 2-8 ºC |
| Mounting | Aqueous (Non-Hardening) |
| Antifade | Yes |
| Counterstain | None |
Documents
- User Guide
- Safety Data Sheet
- VECTASHIELD Fluorochrome Compatibility
- Download CoA
- Datasheet
Product FAQs
What are the advantages of VECTASHIELD PLUS over regular VECTASHIELD (H-1000 & H-1200)?
VECTASHIELD PLUS provides several advantages over the regular VECTASHIELD (H-1000 & H-1200). In particular, VECTASHIELD PLUS media have significantly lower inherent background toning and provide superior signal retention particularly in the far-red spectrum (e.g. Cy5).
Does the coverslip need to be sealed with nail polish or similar compound after mounting?
Similar to other non-setting media, we recommend you to seal the coverslip perimeter if the intention is to store the slides for a period of time.
Does VECTASHIELD PLUS perform equivalently or better with Alexa Fluor dyes compared with the regular VECTASHIELD (H-1000 & H-1200)?
Yes, the antifade mounting media are at least equivalent in signal retention. We have not seen any reduction in performance with any fluorophores evaluated, and with some dyes (i.e. Alexa Fluor 647 and Dylight 649) there are advantages.
Is VECTASHIELD PLUS suitable for super resolution microscopy (SRM)?
We do know that regular VECTASHIELD antifade mounting media (H-1000 & H-1200) are suitable for SRM. However, the formulation for VECTASHIELD PLUS is different from that used for regular VECTASHIELD. Indeed one component of H-1000 and H-1200 that may have been a contributing factor to the underlying blue tone and that appears to play a key role in optimal signal for SRM, has been removed from VECTASHIELD PLUS. Therefore, until we have data to the contrary VECTASHIELD PLUS would not be recommended for SRM.
Citations
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VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult the compatibility table (under the “Protocol/Data Sheet/SDS” tab) to determine if VECTASHIELD will be compatible in your system.
R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182.
The refractive index for VECTASHIELD Mounting Medium is 1.45.
VECTASHIELD Mounting Medium in Super Resolution Microscopy
The optimal medium for super-resolution imaging methods maximizes the lifetime and photoswitching characteristics of fluorophores. VECTASHIELD Mounting Medium has been shown to be compatible with a number of fluorophores used in super resolution methods including STORM, STED, and 3D-SIM imaging, such as Cy5 or Alexa Fluor 647, resulting in greater convenience and reproducibility of the method. (Olivier, N. et al., 2013, Biomedical Optics Express, Vol. 4 No. 6, pp. 885-899; Glushonkov, O. et al., 2018, Scientific Reports, Vol. 8, 8749 (2018) ).
VECTASHIELD Mounting Medium Antifade Comparison
Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD mountants using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer s published results.
