Vector Lab代理Lotus Tetragonolobus Lectin (LTL), Biotinylated

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Lotus Tetragonolobus Lectin (LTL), Biotinylated

B-1325-2

SKU Unit Size Price Qty
B-1325-2 2 mg

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Lotus Tetragonolobus Lectin (LTL), Biotinylated

Description
Specifications
Documents
Product FAQs
Related Products
Citations
Technical Information

Description

Lotus tetragonolobus lectin is a family of closely related glycoproteins that appear to have similar specificities toward α-linked L-fucose containing oligosaccharides. Although many of the binding properties of Lotus lectin are similar to those of Ulex europaeus lectin I, the binding affinities and some specificities for oligosaccharides are significantly different between these fucose-specific lectins.

Biotinylated Lotus tetragonolobus has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

More Information
Unit Size 2 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage Reconstitute by adding 1ml water.The resulting solution will have the following composition:10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.For most applications, we recommend a freshly prepared working solution of5-20 µg/ml in the above buffer.
Recommended Storage 2-8 °C; Store frozen for long term storage
Conjugate Biotinylated
Sugar Specificity Fucose, Arabinose

Documents

  • Lectins in Histochemistry, ELISA, and Western Blot Applications
  • Safety Data Sheet
  • Download CoA
  • Datasheet

Product FAQs

I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is meet. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Related Products

Lotus Tetragonolobus Lectin (LTL), Biotinylated

Lotus Tetragonolobus Lectin (LTL), Unconjugated Catalog #: L-1320-5

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L-fucose Catalog #: S-9007

Lotus Tetragonolobus Lectin (LTL), Biotinylated

Lotus Tetragonolobus Lectin (LTL), Fluorescein Catalog #: FL-1321-2

Citations

Lotus Tetragonolobus Lectin (LTL), Biotinylated Powered by Bioz See more details on Bioz

Technical Information

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: 50 mM - 100 mM L-fucose

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