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Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。
Fluorescein Maleimide
Description
Fluorescein Maleimide is used to label free thiol groups with fluorescein. This fluorescent label can be directly visualized or used as a specific tag that can be detected with anti-fluroescein antibodies. For labeling nucleic acids, thiols can be introduced into DNA, RNA, or oligonucleotides using the 5′ EndTag™ or 3′ EndTag™ nucleic acid labeling systems.
Specifications
| Unit Size | 12 mg |
|---|---|
| Target for Labeling | DNA, Oligonucleotides, PNA, Protein, RNA |
| Recommended Storage | – 20°C to – 80 °C. |
| Molecular Weight | 498.4 |
| Chemical Formula | C27H18N2O8 |
| Maximum Emission | 520 nm |
| Maximum Excitation | 492 nm |
| Required Reactive Group | Thiol Group |
| Tag/Group Incorporated | Fluorescein |
| Safety Title | WARNING: NA – www.P65Warnings.ca.gov |
Documents
- Fluorescein Maleimide (User Guide)
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
Anti-Fluorescein, Biotinylated Catalog #: BA-0601-.5
3' EndTag DNA End Labeling System Catalog #: MB-9002
Biotin (Long Arm) Maleimide Catalog #: SP-1501-12
5' EndTag Nucleic Acid Labeling Kit Catalog #: MB-9001
Citations
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Fluorescein Maleimide is designed for fluoresceinating proteins, nucleic acids or other molecules containing one or more thiol groups. Molecules to be labeled with Fluorescein Maleimide require free thiol groups. In the case of proteins, Fluorescein Maleimide will react with exposed cysteine residues. Alternatively, free thiols may be generated by reducing disulfide bonds or by modifying other reactive groups such as primary amines with compounds like Traut's reagent (2-iminothiolane).
This fluorescent label can be directly detected or used as a hapten and detected with our high affinity antibody reagents. (Excitation maximum at 495 nm; emission maximum at 515 nm).