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Wisteria Floribunda Lectin (WFA, WFL), Fluorescein
Description
The binding specificity of Wisteria floribunda lectin (WFL) is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked α or β to the 3 or 6 position of galactose. This lectin has been used to fractionate lymphocyte populations, and although not mitogenic, elicits the production of lymphokines from murine splenocytes.
Fluorescein labeled WFL has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.
- Excitation maximum: 495 nm
- Emission maximum: 515 nm
- Color: Green
Specifications
| Unit Size | 2 mg |
|---|---|
| Applications | Immunofluorescence, Glycobiology |
| Recommended Usage | The recommended concentration range for use is 5-20 µg/ml. |
| Recommended Storage | 2-8°C |
| Maximum Excitation | 495-500 nm |
| Inhibiting and/or Eluting Sugar | 200 mM N-acetylgalactosamine(S-9001) |
| Maximum Emission | 514-521 nm |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. 0.1 mM CaCl2 |
| Concentration | 2 mg active conjugate/ml |
| Conjugate | Fluorescein |
| Color of Fluorescence | Green |
| Sugar Specificity | N-Acetylgalactosamine |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
Wisteria Floribunda Lectin (WFA, WFL), Biotinylated Catalog #: B-1355-2
Wisteria Floribunda Lectin (WFA, WFL), Unconjugated Catalog #: L-1350-5
N-acetylgalactosamine Catalog #: S-9001
Wisteria Floribunda Lectin (WFA, WFL), Agarose bound Catalog #: AL-1353-2
Citations
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Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine