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Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。
Glycoprotein Eluting Solution for GlcNAc/Chitin Binding Lectins
Description
Vector Laboratories has developed Glycoprotein Elution Solutions in the neutral pH range that maximize the yield of eluted glycoproteins and preserve the activity of the agarose-bound lectins for repeated use. These Glycoprotein Eluting Solutions offer researchers convenience and superior recovery over standard sugar solutions.
Features:
- Preserve the activity of the agarose-bound lectins for repeated use
- All components can be subsequently removed by dialysis
Specifications
| Unit Size | 100 ml |
|---|---|
| Applications | Glycobiology, Affinity Chromatography |
| Recommended Usage | 1)Wash lectin agarose column to remove unbound proteins with HEPES- or TRIS-Buffered saline (HBS, TBS), pH 7.5, until OD280nm reaches background level. 2)Apply Glycoprotein Eluting Solution and allow to flow through the column by gravity.Note: Do not apply pressure to speed elution because this may reduce the percent recovery of the bound glycoprotein.Complete elution may take up to 10 column volumes.* 3) Following elution, the agarose lectin column is ready for reuse after equilibrating with 2-4 column volumes of HBS or TBS, pH 7.5. *These results may not be typical for other glycoproteins, since oligosaccharide structures of glycoproteins vary significantly.The eluting solution has a high osmolarity.If downstream applications are adversely affected by salt, eluted glycoproteins may require dialysis or gel filtration. |
| Recommended Storage | Room Temperature |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
What is the primary composition of the Vector Labs glycoprotein eluting solutions? Do any of these contain protein?
Related Products
Glycoprotein Eluting Solution for Mannose/Glucose Binding Lectins Catalog #: ES-1100-100
Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins Catalog #: ES-2100-100
Glycoprotein Eluting Solution for Sialic Acid Binding Lectins Catalog #: ES-7100-100
Technical Information
Glycoproteins are frequently isolated and purified from protein mixtures using columns of agarose-bound lectins. After applying a protein mixture, the agarose-lectin column is washed free of unwanted proteins and the glycoprotein bound to the lectin is eluted with a sugar that inhibits binding. Unfortunately, achieving complete elution with a simple sugar solution can be difficult.
