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Maackia Amurensis Lectin I (MAL I), Biotinylated
Description
Maackia amurensis lectin I binds gal (β-1,4) glcNAc but tolerates substitution of N-acetyllactosamine with sialic acid at the 3 position of galactose. However, MAL I does not appear to bind this structure when substitution with sialic acid is on the 6 position of galactose.
Biotinylated Maackia amurensis lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
Specifications
| Unit Size | 2 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
| Recommended Usage | Reconstitute in 1 ml water. The resulting solution will be: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% NaN3, 0.1 mM CaCl2, 0.01 mM MnCl2. |
| Recommended Storage | 2-8 °C; Store frozen for long term storage |
| Inhibiting and/or Eluting Sugar | 200 mM lactose (S-9004). |
| Conjugate | Biotinylated |
| Sugar Specificity | Galactose, Lactose |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
Related Products
Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins Catalog #: ES-2100-100
Maackia Amurensis Lectin I (MAL I), Fluorescein Catalog #: FL-1311-2
Maackia Amurensis Lectin I (MAL I), Unconjugated Catalog #: L-1310-5
Galactose Catalog #: S-9003
Lactose Catalog #: S-9004
Citations
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Geisler, C and Jarvis, D.L. Glycobiology, vol. 21, no. 8 pp. 988-993, 2011
Inhibiting Sugar: 200 mM lactose