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Concanavalin A (Con A), Biotinylated
Description
Con A recognizes α-linked mannose present as part of a “core oligosaccharide” in many serum and membrane glycoproteins.
Biotinylated Concanavalin A has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
Specifications
| Unit Size | 5 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
| Recommended Usage | For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer. |
| Recommended Storage | 2-8 °C; Store frozen for long term storage |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.01 mM MnCl2, 0.1 mM CaCl2, 0.08% sodium azide, 10 mM alpha methylmannoside. |
| Concentration | 5 mg/ml |
| Conjugate | Biotinylated |
| Sugar Specificity | Mannose, Glucose |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
Related Products
α-methylmannoside Catalog #: S-9005
α-methylglucoside Catalog #: S-9006
Concanavalin A (Con A), Unconjugated Catalog #: L-1000-500
Concanavalin A (Con A), Fluorescein Catalog #: FL-1001-25
Concanavalin A (Con A), Rhodamine Catalog #: RL-1002-25
Citations
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Con A requires calcium or manganese ions at each of its four saccharide binding sites. Although these divalent metal ions are bound tightly to the polypeptide structure, buffers which can bind calcium (such as phosphate) generally should be avoided in diluting Con A, since a gradual loss in activity may occur.
This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN® ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside