Vector Lab代理Methyl Green Counterstain

发表于由

Vector Labs代理，欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

Methyl Green Counterstain

H-3402-500

SKU Unit Size Price Qty
H-3402-500 500 ml

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Methyl Green Counterstain

Description
Specifications
Documents
Product FAQs
Related Products
Citations
Technical Information

Description

Vector Methyl Green stains cell nuclei light green. It is an excellent alternative in multiple labeling when hematoxylin obscures the substrate colors.

Features:

  • Simple, two-step procedure
  • Suitable for use with a wide range of enzyme substrates
  • Suitable for use with non-aqueous mountants

Specifications

More Information
Unit Size 500 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization
Format Ready-to-Use

Documents

  • User Guide
  • Counterstain/Substrate Compatibility Chart
  • Safety Data Sheet
  • Download CoA
  • Datasheet

Product FAQs

I am using Methyl Green (H-3402) as a counterstain for my IHC, but the stain is a little weak. What suggestions do you have to increase the staining intensity?

Methyl Green counterstain does take a little bit of optimization in some applications. We would suggest heating a volume of the counterstain (~300 ml) to 60 °C and add to a Coplin jar or similar glass staining rack, and submerse the slides. This approach facilitates a better uptake of stain into the section compared with placing the slide on a heated surface and placing drops of stain onto the section. Following the incubation time (~3-5 min), remove slides and wash in tap water. Omit the acetone, acetic acid rinse step described in the instructions and move the slides directly into 95% ethanol for the dehydration, clearing and mounting process. This methodology will retain more stain in the nuclei and hence produce greater intensity.

Related Products

Methyl Green Counterstain

Hematoxylin QS Counterstain Catalog #: H-3404-100

Methyl Green Counterstain

VectaMount® Permanent Mounting Medium Catalog #: H-5000-60

Methyl Green Counterstain

Nuclear Fast Red Counterstain Catalog #: H-3403-500

Methyl Green Counterstain

Hematoxylin Counterstain Catalog #: H-3401-500

Citations

Methyl Green Counterstain Powered by Bioz See more details on Bioz

Technical Information

Counterstain/Substrate Compatibility Table

This table is designed as a reference to determine the optimal counterstain/substrate combination for your application.

Methyl Green Counterstain

Home All Products Counterstains and Special Stains Methyl Green Counterstain