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Vector Labs 公司是世界著名的生物学检测试剂公司,七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命,该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统,并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展,以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商,并提供大量的新产品,如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。
ImmPACT® VIP Substrate, Peroxidase (HRP)
Description
The ImmPACT VIP HRP substrate produces an intense, violet (purple) reaction product, and can be used as an alternative to DAB or as a second color for multiple antigen labeling. This product generates an intense reaction product that is 2-4 times more sensitive than the Vector VIP substrate kit (SK-4600).
Features:
- 2-4 fold greater sensitivity than original substrate kit
- Optimized for IHC/ICC/ISH and blots
- Permanent mounting
- Ideal for single and multiple labeling
- Working solutions stable for up to 14 days at 4 °C
- Supplied as 120 ml of diluent and stock solutions in dropper bottles
Specifications
Unit Size | 120 ml |
---|---|
Applications | Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot |
Detection Enzyme(s) | Peroxidase |
Substrate Reaction Product | Precipitating Reaction Product |
Chromogen Color | Purple |
Microscopy | Brightfield, Darkfield, Electron, Spectral Imaging |
Additional Substrate Properties | Contrast with Tissue Pigments, Multiple Labeling |
Mounting | Non-Aqueous (Permanent) |
Kit Components
Kit Contents:
- 120 ml ImmPACT VIP Diluent
- 3 ml ImmPACT VIP Reagent 1
- 2.5 ml ImmPACT VIP Reagent 2
- 2.5 ml ImmPACT VIP Reagent 3
- 3 ml ImmPACT VIP Reagent 4
Documents
- Enzyme Substrates Brochure
- Enzyme Substrate Combinations Chart
- Counterstain/Substrate Compatibility Chart
- User Guide
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
BLOXALL® Endogenous Blocking Solution, Peroxidase and Alkaline Phosphatase Catalog #: SP-6000-100
Methyl Green Counterstain Catalog #: H-3402-500
Hematoxylin Counterstain Catalog #: H-3401-500
Hematoxylin QS Counterstain Catalog #: H-3404-100
VectaMount® Permanent Mounting Medium Catalog #: H-5000-60
Citations
Technical Information
Vector VIP and ImmPACT VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina (1). Sections stained with either substrate can be viewed by darkfield and electron microscopy. Vector VIP and ImmPACT VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted.
This kit consists of 120 ml of diluent and concentrated stock solutions of ImmPACT VIP reagents in convenient dropper bottles. Working solutions are stable for up to 14 days at 4 °C.
Product review on Biocompare.com.
1. Duncan SM, Seigel GM. High-contrast enzymatic immunohistochemistry of pigmented tissues. J Biol Methods2016;3(3):e47. doi: 10.14440/jbm.2016.122