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Goat Anti-Rat IgG Antibody (H+L), Biotinylated
Description
Biotinylated Goat Anti-Rat IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.
Features:
- Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species. (For mouse tissue applications, please see anti-rat, mouse adsorbed, secondary antibodies.)
- Recognizes both heavy and light chains (H+L)
- Supplied in solution
Specifications
Unit Size | 1.5 mg |
---|---|
Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
Concentration | 1.5 mg active conjugate/ml |
Recommended Storage | 2-8 °C; Store frozen for long term storage |
Solution | 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin. |
Recommended Usage | The recommended concentration range for use is 2-10 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. NOTE: This product is raised in goat and is not the same as that contained in the VECTASTAIN ABC Kits for the detection of rat IgG which is raised in rabbit. |
Target Species | Rat |
Conjugate | Biotinylated |
Host Species | Goat |
Format | Concentrate |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
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Citations
Technical Information
With some exceptions the recommended dilution for most applications is 1:200.