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Horse Anti-Mouse/Rabbit IgG Antibody (H+L) (Universal), Biotinylated
Description
This biotinylated universal secondary antibody binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies. This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.
Features:
- Recognizes both heavy and light chains (H+L)
- Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
- Can be used for tissue and cell staining, ELISAs, and blots
- Included in the VECTASTAIN® ABC kits
- Supplied as solution
- Recommended dilution for most applications is 1:50
- This pan-specific, biotinylated “universal” secondary antibody is specially formulated to recognize IgG (H+L) primary antibodies from mouse and rabbit
Note: This product is the same biotinylated secondary antibody included in the VECTASTAIN® Elite ABC Kit (Universal; PK-6200) and VECTASTAIN® ABC-AP Kit (Universal; AK-5200), but is not the same as that provided in the VECTASTAIN®Universal Quick Kit, PK-8800.
Specifications
| Unit Size | 2.1 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
| Concentration | 1.05 mg/ml |
| Recommended Storage | 2-8 °C; Store frozen for long term storage |
| Solution | 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin. |
| Recommended Usage | The recommended concentration range for use is 10-20 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Note:This product is the same solution as that provided in the VECTASTAIN® Universal ABC Elite® kit (cat. no. PK-6200) and VECTASTAIN® Universal ABC-AP kit (cat. no. AK-5200). |
| Target Species | Universal (Mouse/Rabbit) |
| Conjugate | Biotinylated |
| Host Species | Horse |
| Format | Concentrate |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
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Citations
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The high staining quality and sensitivity achieved using this product is equivalent to that obtained with either our biotinylated anti-mouse IgG (H+L) or biotinylated anti-rabbit IgG (H+L) used individually. The convenience of using a single biotinylated secondary antibody for both species of primary and the elimination of possible mistakes in using the wrong secondary antibody is an advantage of this product.