AAT-Texas Red-streptavidin conjugate
Upgrade: iFluor™ 594-streptavidin conjugate
See also: Bioconjugation, Biotin and Streptavidin, Flow Cytometry Reagents
Molecular weight
52000
|
Correction Factor (260 nm)
0.23
|
Correction Factor (280 nm)
0.36
|
Correction Factor (565 nm)
0.4472
|
Extinction coefficient (cm -1 M -1)
1160001
|
Excitation (nm)
586
|
Emission (nm)
603
|
Quantum yield
0.931, 0.352
|
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This Texas Red-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with Texas Red covalently attached (as the fluorescent label). Our Texas Red-streptavidin conjugate was prepared using AAT Bioquest’s proprietary labeling technology. It demonstrated much brighter signal compared to the similar Texas Red-streptavidin conjugates from other commercial sources, thus can significantly increase assay sensitivities. It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Texas Red filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Texas Red®-streptavidin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 1.923 µL | 9.615 µL | 19.231 µL | 96.154 µL | 192.308 µL |
5 mM | 384.615 nL | 1.923 µL | 3.846 µL | 19.231 µL | 38.462 µL |
10 mM | 192.308 nL | 961.538 nL | 1.923 µL | 9.615 µL | 19.231 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
链霉亲和素缀合物与生物素缀合物一起广泛用于特异性检测各种蛋白质、蛋白质基序、核酸和其他分子,因为链霉亲和素对生物素具有非常高的结合亲和力。该德克萨斯红-链霉抗生物素偶联物包含链霉抗生物素(作为生物素结合蛋白)与德克萨斯红共价连接(作为荧光标记)。我们的 Texas Red-streptavidin 偶联物是使用 AAT Bioquest 的专有标记技术制备的。与来自其他商业来源的类似德州红-链霉亲和素偶联物相比,它显示出更亮的信号,因此可以显着提高检测灵敏度。当与生物素化一抗结合使用时,它通常用作间接免疫荧光染色的第二步试剂。它是使用流式细胞仪和 Texas Red 过滤器组进行基于生物素-链霉亲和素的生物测定和测试的宝贵工具。多种互补的生物素化试剂可从众多商业供应商处获得。
普通储备溶液制备
表 1.将特定质量的Texas Red®-链霉亲和素偶联物重新配制到给定浓度所需的水体积。请注意,体积仅用于制备储备溶液。有关适当的实验/生理缓冲液,请参阅示例实验协议。
0.1毫克 | 0.5毫克 | 1毫克 | 5毫克 | 10毫克 | |
1毫米 | 1.923 µL | 9.615 µL | 19.231 µL | 96.154 µL | 192.308 µL |
5毫米 | 384.615 毫升 | 1.923 µL | 3.846 µL | 19.231 µL | 38.462 µL |
10毫米 | 192.308 毫升 | 961.538 毫升 | 1.923 µL | 9.615 µL | 19.231 µL |
摩尔浓度计算器
输入任意两个值(质量、体积、浓度)来计算第三个。
Spectrum
光谱
Spectral properties 光谱特性
Correction Factor (260 nm) | 0.23 |
Correction Factor (280 nm) | 0.36 |
Correction Factor (565 nm) | 0.4472 |
Extinction coefficient (cm -1 M -1) | 1160001 |
Excitation (nm) | 586 |
Emission (nm) | 603 |
Quantum yield | 0.931, 0.352 |
Images
Figure 1. HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by Texas Red®-streptavidin conjugate (Red). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
图1. HeLa 细胞与小鼠抗微管蛋白和生物素山羊抗小鼠 IgG 孵育,然后是 Texas Red®-链霉亲和素偶联物(红色)。细胞核用 Hoechst 33342(蓝色,Cat#17530)染色。
Citations
View all 1 citations: Citation Explorer
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989–1996
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989–1996
References 参考文献
View all 47 references: Citation Explorer
A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647
Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50
Authors: Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF.
Journal: BMC Biochem (2010): 50
Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label
Authors: Sun M, Du L, Gao S, Bao Y, Wang S.
Journal: Steroids (2010): 400
Authors: Sun M, Du L, Gao S, Bao Y, Wang S.
Journal: Steroids (2010): 400
Multimodality nuclear and fluorescence tumor imaging in mice using a streptavidin nanoparticle
Authors: Liang M, Liu X, Cheng D, Liu G, Dou S, Wang Y, Rusckowski M, Hnatowich DJ.
Journal: Bioconjug Chem (2010): 1385
Authors: Liang M, Liu X, Cheng D, Liu G, Dou S, Wang Y, Rusckowski M, Hnatowich DJ.
Journal: Bioconjug Chem (2010): 1385
Site-dependent excited-state dynamics of a fluorescent probe bound to avidin and streptavidin
Authors: Furstenberg A, Kel O, Gradinaru J, Ward TR, Emery D, Bollot G, Mareda J, Vauthey E.
Journal: Chemphyschem (2009): 1517
Authors: Furstenberg A, Kel O, Gradinaru J, Ward TR, Emery D, Bollot G, Mareda J, Vauthey E.
Journal: Chemphyschem (2009): 1517
Influence of streptavidin on the absorption and fluorescence properties of cyanine dyes
Authors: Luschtinetz F, Dosche C, Kumke MU.
Journal: Bioconjug Chem (2009): 576
Authors: Luschtinetz F, Dosche C, Kumke MU.
Journal: Bioconjug Chem (2009): 576
Fluorescent nanoscale detection of biotin-streptavidin interaction using near-field scanning optical microscopy
Authors: Park HK, Gokarna A, Hulme JP, Park HG, Chung BH.
Journal: Nanotechnology (2008): 235103
Authors: Park HK, Gokarna A, Hulme JP, Park HG, Chung BH.
Journal: Nanotechnology (2008): 235103
Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives
Authors: Ebner A, Marek M, Kaiser K, Kada G, Hahn CD, Lackner B, Gruber HJ.
Journal: Methods Mol Biol (2008): 73
Authors: Ebner A, Marek M, Kaiser K, Kada G, Hahn CD, Lackner B, Gruber HJ.
Journal: Methods Mol Biol (2008): 73