离子去污剂
离子除垢剂通常含有阴离子或阳离子头部基团及一个净电荷。其疏水尾部不是十二烷基硫酸钠(SDS)和十六烷基三甲基溴化铵(CTAB)等可见的直链烃,就是胆汁酸盐等包含的刚性甾体基团。离子除垢剂溶解膜蛋白非常有效,但基本都会导致一定程度的变性。胆汁酸盐是一种阴离子除垢剂,其主链由刚性甾体基团组成,例如胆酸钠盐和脱氧胆酸钠盐等。这类分子的平面结构使其具有一个极性面和一个非极性面,因此,其CMC较高,胶束较小,易于通过透析去除。
非离子去污剂
非离子除垢剂含不带电的亲水头部基团,该基团由BRIJR和TRITON?等除垢剂中的聚氧乙烯部分或辛基葡糖苷和十二烷基麦芽糖苷等中的糖苷基团组成。非离子除垢剂会破坏脂质-脂质和脂质-蛋白互作,但不会破坏蛋白-蛋白的互作,所以是公认的非变性除垢剂,因此广泛应用于分离生物活性形式的膜蛋白。另外,与离子除垢剂不同,非离子除垢剂的胶束尺寸受盐的影响很小。
两性离子去污剂
两性离子除垢剂兼具离子和非离子除垢剂的特性。与非离子除垢剂一样,两性离子除垢剂不具有净电荷,缺乏导电性和电泳迁移率,且不与离子交换树脂结合。因此是离子交换层析、电泳和等电聚焦中常用的非离子除垢剂的替代物。然而,像离子洗涤剂一样,它们能有效地打破蛋白质与蛋白质之间相互作用。另外,甾体类两性离子除垢剂(如CHAPS)相比直链两性离子除垢剂(如十二烷基二甲基二氧化胺)变性能力通常更弱。
P1379
TWEEN ? 20, viscous liquid
X100
Triton? X-100, laboratory grade
L3771
十二烷基硫酸钠, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)
T8787
Triton? X-100, for molecular biology
P9416
TWEEN ? 20, for molecular biology, viscous liquid
P1754
TWEEN ? 80, viscous liquid
P7949
TWEEN ? 20, BioXtra, viscous liquid
PVP40
聚乙烯吡咯烷酮, average mol wt 40,000
D6750
脱氧胆酸钠, ≥97% (titration)
L4509
十二烷基硫酸钠, ReagentPlus?, ≥98.5% (GC)
436143
十二烷基硫酸钠, ACS reagent, ≥99.0%
I6758
IPTG, ≥99% (TLC), ≤0.1% Dioxane
T9284
Triton? X-100, BioXtra
P2443
Pluronic? F-127, powder, BioReagent, suitable for cell culture
93443
Triton? X-100 溶液, BioUltra, for molecular biology, ~10% in H2O
H5882
溴化十六烷基三甲铵, ≥98%
71736
十二烷基硫酸钠 溶液, BioUltra, for molecular biology, 10% in H2O
I8896
IGEPAL? CA-630, for molecular biology
A6426
消泡剂 204, mixture of organic polyether dispersions
O8380
1-辛烷磺酸 钠盐, ≥98%
I3021
IGEPAL? CA-630, viscous liquid
H2766
1-庚烷磺酸钠
H6269
溴化十六烷基三甲铵, for molecular biology, ≥99%
P2287
TWEEN ? 20, viscous liquid, suitable for cell culture
P9769
十乙二醇单十二烷基醚, nonionic surfactant
D141
毛地黄皂苷, Used as non-ionic detergent
75746
十二烷基硫酸钠, ≥99.0% (GC), dust-free pellets
P4780
TWEEN ? 80, suitable for cell culture, suitable for insect cell culture, viscous liquid
88315
Thesit?, for membrane research
59924
聚山梨酯80, tested according to Ph. Eur.
89510
聚(乙二醇), BioUltra, 8,000
P5556
Poloxamer 188 溶液, 10%, sterile-filtered, BioReagent, suitable for insect cell culture
G4376
L -氧化谷胱甘肽, ≥98% (HPLC)
C5135
Kolliphor? EL, pH range 6.0-8.0
P8136
聚乙烯醇, 87-90% hydrolyzed, average mol wt 30,000-70,000
12060
苯扎氯铵, ≥95.0% (T)
C3023
CHAPS水合物, ≥98% (HPLC)
T7505
四甲基氢氧化铵 五水合物, ≥97%
H26456
二乙二醇, ReagentPlus?, 99%
59920C
EX-CELL?止泡剂, gamma irradiated
71725
十二烷基硫酸钠, BioUltra, for molecular biology, ≥99.0% (GC)
30970
脱氧胆酸钠, BioXtra, ≥98.0% (dry matter, NT)
H5269
己烷磺酸钠, ≥98% (elemental analysis)
K4894
Kolliphor? HS 188, solid
42966
Kolliphor? HS 15
P5413
聚(乙二醇), for molecular biology, average mol wt 8,000
S6760
Span? 80, nonionic surfactant
74882
1-辛烷磺酸钠 一水合物, suitable for ion pair chromatography, LiChropur?, ≥99.0% (T)
A5757
消泡剂 B 乳液, aqueous-silicone emulsion
B4184
Brij? L23 溶液, 30 % (w/v) in H2O
D8638
十二烷基三甲基溴化铵, ≥98%
40236
十二烷基二甲基氧化胺 溶液, ~30% in H2O
47036
皂素, for molecular biology, used as non-ionic surfactant
X100RS
Triton? X-100(还原型)
P8074
TWEEN ? 80, BioXtra, viscous liquid
L5750
十二烷基硫酸钠, 92.5-100.5% based on total alkyl sulfate content basis
A8011
消泡剂C乳液, aqueous-silicone emulsion
X114
Triton? X-114, laboratory grade
L6026
十二烷基硫酸钠, BioXtra, ≥99.0% (GC)
B8879
苄索氯铵, ≥97% (titration), ≥98% (HPLC)
05030
十二烷基硫酸钠 溶液, BioUltra, for molecular biology, 20% in H2O
T4009
牛磺胆酸 钠盐 水合物, ≥95% (HPLC)
T0875
牛磺脱氧胆酸钠 水合物, ≥95% (HPLC)
L9150
N -月桂酰肌氨酸 钠盐, detergent for use in cell lysis
P3015
聚(乙二醇), average mol wt 200
A8311
消泡剂 204, aqueous emulsion for bacterial and mammalian systems
15S9
TERGITOL烷氧基聚乙烯氢氧基乙醇(非离子表面活性剂)?, Type 15-S-9
03394
聚(乙二醇), tested according to Ph. Eur., 6,000
30326
EMPIGEN? BB去污剂, ~30% active substance
86339
牛胆酸钠 水合物, ≥97.0% (TLC)
84510
皂素, used as non-ionic surfactant
G7132
甘胆酸钠 水合物, ≥95% (TLC)
51832
1-庚烷磺酸钠 一水合物, suitable for ion pair chromatography, LiChropur?, ≥99.0% (T)
52862
1-己烷磺酸钠 一水合物, suitable for ion pair chromatography, LiChropur?, ≥99.0% (T)
10783641001
聚乙二醇1500, Mr ~1500, pkg of 10 × 4 mL, solution
L5125
N -月桂酰肌氨酸 钠盐, ≥94%
H9151
溴化十六烷基三甲铵, BioXtra, ≥99%
15759
Kolliphor? P 188
G0759
甘氨鹅脱氧胆酸钠
16758
泊洛沙姆407, purified, non-ionic