GAPDH Mouse Monoclonal Antibody [Clone ID: OTI2D9]GAPDH小鼠单克隆抗体[克隆ID：OTI2D9]

发表于由
Product Data
Clone Name OTI2D9
Applications WB
Recommend Dilution WB 1:2000
Reactivity Human, Monkey, Mouse, Rat, Dog
Host Mouse
Clonality Monoclonal
Immunogen Full length human recombinant protein of human GAPDH (NP_002037) produced in HEK293T cell.
Isotype IgG1
Formulation PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 1 mg/ml
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Condition Store at -20°C as received.
Predicted Protein Size 35.9 kDa
Gene Name glyceraldehyde-3-phosphate dehydrogenase
Background The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
 Reference Data
Protein Families ES Cell Differentiation/IPS
Protein Pathways Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

The use of this Antibodies has been cited in the following citations:
Progressive Characterization of Visual Phenotype in Bardet-Biedl Syndrome Mutant Mice ,Kretschmer, V;Patnaik, SR;Kretschmer, F;Chawda, MM;Hernandez-Hernandez, V;May-Simera, HL;, Invest. Ophthalmol. Vis. Sci. 2019 ,PubMed ID 30901771 [GAPDH]
Znhit1 controls intestinal stem cell maintenance by regulating H2A.Z incorporation ,Zhao, B;Chen, Y;Jiang, N;Yang, L;Sun, S;Zhang, Y;Wen, Z;Ray, L;Liu, H;Hou, G;Lin, X;, Nat Commun 2019 ,PubMed ID 30842416 [GAPDH]
SIRT1 participates in the response to methylglyoxal-dependent glycative stress in mouse oocytes and ovary ,Di Emidio, G;Santini, SJ;D'Alessandro, AM;Vetuschi, A;Sferra, R;Artini, PG;Carta, G;Falone, S;Amicarelli, F;Tatone, C;, Biochim Biophys Acta Mol Basis Dis 2019 ,PubMed ID 30771486 [GAPDH]
MicroRNA-Based Prophylaxis in a Mouse Model of Cirrhosis and Liver Cancer ,Callegari, E;Domenicali, M;Shankaraiah, RC;D'Abundo, L;Guerriero, P;Giannone, F;Baldassarre, M;Bassi, C;Elamin, BK;Zagatti, B;Ferracin, M;Fornari, F;Altavilla, G;Blandamura, S;Silini, EM;Gramantieri, L;Sabbioni, S;Negrini, M;, Mol Ther Nucleic Acids 2018 ,PubMed ID 30641476 [GAPDH]
Selective CB2 inverse agonist JTE907 drives T cell differentiation towards a Treg cell phenotype and ameliorates inflammation in a mouse model of inflammatory bowel disease ,Gentili, M;Ronchetti, S;Ricci, E;Di Paola, R;Gugliandolo, E;Cuzzocrea, S;Bereshchenko, O;Migliorati, G;Riccardi, C;, Pharmacol. Res. 2018 ,PubMed ID 30552973 [GAPDH]
Muscle activity prevents the uncoupling of mitochondria from Ca2+ release units induced by ageing and disuse ,Pietrangelo, L;Michelucci, A;Ambrogini, P;Sartini, S;Guarnier, FA;Fusella, A;Zamparo, I;Mammucari, C;Protasi, F;Boncompagni, S;, Arch. Biochem. Biophys. 2018 ,PubMed ID 30578752 [GAPDH]
Incomplete Suppression of HIV-1 by SAMHD1 Permits Efficient Macrophage Infection ,Plitnik, T;Sharkey, M;Mahboubi, B;Kim, B;Stevenson, M;, PAI 2018 [GAPDH]
An AAV dual vector strategy ameliorates the Stargardt phenotype in adult Abca4-/- mice ,McClements, ME;Barnard, AR;Singh, MS;Charbel Issa, P;Jiang, Z;Radu, RA;MacLaren, RE;, Hum. Gene Ther. 2018 ,PubMed ID 30381971 [GAPDH]
Aortic constriction induces hypertension and cardiac hypertrophy via (pro)renin receptor activation and the PLC‑β3 signaling pathway ,Wu, J;Zhang, C;Liu, C;Zhang, A;Li, A;Zhang, J;Zhang, Y;, Mol Med Rep 2018 ,PubMed ID 30431106 [GAPDH]
Leucine-rich repeat kinase 2 controls protein kinase A activation state through phosphodiesterase 4 ,Russo, I;Di Benedetto, G;Kaganovich, A;Ding, J;Mercatelli, D;Morari, M;Cookson, MR;Bubacco, L;Greggio, E;, J Neuroinflammation 2018 ,PubMed ID 30368241 [GAPDH]
Trehalose Activates CRE-Dependent Transcriptional Signaling in HT22 Mouse Hippocampal Neuronal Cells: A Central Role for PKA Without cAMP Elevation ,Maronde, E;, Front Mol Neurosci 2018 ,PubMed ID 30405349 [GAPDH]
The Elevated ASK1 Expression Inhibits Proliferation and Invasion in Gastric Cancer HGC-27 Cells ,Wan, X;Shi, L;Ma, X;Tang, H;Wu, G;, Anat Rec (Hoboken) 2018 ,PubMed ID 30324658 [GAPDH]
Efflux excretion of bisdemethoxycurcumin-O-glucuronide in UGT1A1-overexpressing HeLa cells: Identification of breast cancer resistance protein (BCRP) and multidrug resistance-associated proteins 1 (MRP1) as the glucuronide transporters ,Yang, J;Zhang, B;Qin, Z;Li, S;Xu, J;Yao, Z;Zhang, X;Gonzalez, FJ;Yao, X;, Biofactors 2018 ,PubMed ID 30334318 [GAPDH]
Sucrosomial® Iron Supplementation in Mice: Effects on Blood Parameters, Hepcidin, and Inflammation ,Asperti, M;Gryzik, M;Brilli, E;Castagna, A;Corbella, M;Gottardo, R;Girelli, D;Tarantino, G;Arosio, P;Poli, M;, Nutrients 2018 ,PubMed ID 30241424 [GAPDH]
Cysteine thiol oxidation on SIRT2 regulates inflammation in obese mice with sepsis ,Wang, X;Buechler, NL;Long, DL;Furdui, CM;Yoza, BK;McCall, CE;Vachharajani, V;, Inflammation 2018 ,PubMed ID 30203196 [GAPDH]
The roles of breast cancer resistance protein (BCRP/ABCG2) and multidrug resistance-associated proteins (MRPs/ABCCs) in the excretion of cycloicaritin-3-O-glucoronide in UGT1A1-overexpressing HeLa cells ,Li, S;Xu, J;Yao, Z;Hu, L;Qin, Z;Gao, H;Krausz, KW;Gonzalez, FJ;Yao, X;, Chem. Biol. Interact. 2018 ,PubMed ID 30237061 [GAPDH]
Downregulation of FOXK2 is associated with poor prognosis in patients with gastric cancer ,Liu, X;Wei, X;Niu, W;Wang, D;Wang, B;Zhuang, H;, Mol Med Rep 2018 ,PubMed ID 30221666 [GAPDH]
Regulation of the oncogenic phenotype by the nuclear body protein ZC3H8 ,Schmidt, JA;Danielson, KG;Duffner, ER;Radecki, SG;Walker, GT;Shelton, A;Wang, T;Knepper, JE;, BMC Cancer 2018 ,PubMed ID 30041613 [GAPDH]
The RNA-binding protein YBX1 regulates epidermal progenitors at a posttranscriptional level ,Kwon, E;Todorova, K;Wang, J;Horos, R;Lee, KK;Neel, VA;Negri, GL;Sorensen, PH;Lee, SW;Hentze, MW;Mandinova, A;, Nat Commun 2018 ,PubMed ID 29712925 [GAPDH]
Aniseed ( Pimpinella anisum L.) essential oil reduces pro-inflammatory cytokines and stimulates mucus secretion in primary airway bronchial and tracheal epithelial cell lines ,Iannarelli, R;Marinelli, O;Morelli, M;Santoni, G;Amantini, C;Nabissi, M;Maggi, F;, Industrial Crops and Products 2018 [GAPDH]
SKLB-23bb, A HDAC6-Selective Inhibitor, Exhibits Superior and Broad-Spectrum Antitumor Activity via Additionally Targeting Microtubules ,Wang, F;Zheng, L;Yi, Y;Yang, Z;Qiu, Q;Wang, X;Yan, W;Bai, P;Yang, J;Li, D;Pei, H;Niu, T;Ye, H;Nie, C;Hu, Y;Yang, S;Wei, Y;Chen, L;, Mol. Cancer Ther. 2018 ,PubMed ID 29610282 [GAPDH]
Identification of Miro as a mitochondrial receptor for myosin XIX ,Bähler, M;Oeding, S;Majstrowicz, K;Hu, X;Schwarz, V;Freitag, A;Honnert, U;Nikolaus, P;, bioRxiv 2018 [GAPDH]
NOD2-RIP2 contributes to the inflammatory responses of mice in vivo to Streptococcus pneumoniae ,Zheng, Y;Shang, F;An, L;Zhao, H;Liu, X;, Neurosci. Lett. 2018 ,PubMed ID 29408491 [GAPDH]
SMAD4 feedback regulates the canonical TGF-β signaling pathway to control granulosa cell apoptosis ,Du, X;Pan, Z;Li, Q;Liu, H;Li, Q;, Cell Death Dis 2018 ,PubMed ID 29396446 [GAPDH]
Structural properties and gene-silencing activity of chemically modified DNA-RNA hybrids with parallel orientation ,Habibian, M;Yahyaee-Anzahaee, M;Lucic, M;Moroz, E;Martín-Pintado, N;Di Giovanni, LD;Leroux, JC;Hall, J;González, C;Damha, MJ;, Nucleic Acids Res. 2018 ,PubMed ID 29373740 [GAPDH]
Enhancing mitochondrial proteostasis reduces amyloid-β proteotoxicity ,Sorrentino, V;Romani, M;Mouchiroud, L;Beck, JS;Zhang, H;D'Amico, D;Moullan, N;Potenza, F;Schmid, AW;Rietsch, S;Counts, SE;Auwerx, J;, Nature 2017 ,PubMed ID 29211722 [GAPDH]
Effect of miR-204&211 and RUNX2 control on the fate of human mesenchymal stromal cells ,Sacchetti, B;Fatica, A;Sorci, M;Sorrentino, A;Signore, M;Cerio, A;Felicetti, F;Feo, A;Pelosi, E;Caré, A;Pescarmona, E;Gordeladze, JO;Valtieri, M;, Regen Med Res 2017 ,PubMed ID 29206625 [GAPDH]
Shear stress induces noncanonical autophagy in intestinal epithelial monolayers ,Kim, SW;Ehrman, J;Ahn, MR;Kondo, J;Lopez, AAM;Oh, YS;Kim, XH;Crawley, SW;Goldenring, JR;Tyska, MJ;Rericha, EC;Lau, KS;, Mol. Biol. Cell 2017 ,PubMed ID 28855375 [GAPDH]
FMNL2 destabilises COMMD10 to activate NF-κB pathway in invasion and metastasis of colorectal cancer ,Yang, SS;Li, XM;Yang, M;Ren, XL;Hu, JL;Zhu, XH;Wang, FF;Zeng, ZC;Li, JY;Cheng, ZQ;Liao, WT;Ding, YQ;Guan, J;Liang, L;, Br. J. Cancer 2017 ,PubMed ID 28817833 [GAPDH]
Antioxidant Treatment Reduces Formation of Structural Cores and Improves Muscle Function in RYR1(Y522S/WT) Mice ,Michelucci, A;De Marco, A;Guarnier, FA;Protasi, F;Boncompagni, S;, Oxid Med Cell Longev 2017 ,PubMed ID 29062463 [GAPDH]
Anti-Inflammatory Pyranochalcone Derivative Attenuates LPS-Induced Acute Kidney Injury via Inhibiting TLR4/NF-κB Pathway ,Shi, M;Zeng, X;Guo, F;Huang, R;Feng, Y;Ma, L;Zhou, L;Fu, P;, Molecules 2017 ,PubMed ID 28994737 [GAPDH]
Codon-Optimized RPGR Improves Stability and Efficacy of AAV8 Gene Therapy in Two Mouse Models of X-Linked Retinitis Pigmentosa ,Fischer, MD;McClements, ME;Martinez-Fernandez de la Camara, C;Bellingrath, JS;Dauletbekov, D;Ramsden, SC;Hickey, DG;Barnard, AR;MacLaren, RE;, Mol. Ther. 2017 ,PubMed ID 28549772 [GAPDH]
Strenuous exercise triggers a life-threatening response in mice susceptible to malignant hyperthermia ,Michelucci, A;Paolini, C;Boncompagni, S;Canato, M;Reggiani, C;Protasi, F;, FASEB J. 2017 ,PubMed ID 28465322 [GAPDH]
miR-146a suppresses STAT3/VEGF pathways and reduces apoptosis through IL-6 signaling in primary human retinal microvascular endothelial cells in high glucose conditions ,Ye, EA;Steinle, JJ;, Vision Res. 2017 ,PubMed ID 28433754 [GAPDH]
Hepatitis C virus infection inhibits a Src-kinase regulatory phosphatase and reduces T cell activation in vivo ,Bhattarai, N;McLinden, JH;Xiang, J;Mathahs, MM;Schmidt, WN;Kaufman, TM;Stapleton, JT;, PLoS Pathog 2017 ,PubMed ID 28235043 [GAPDH]
Axitinib induces senescence-associated cell death and necrosis in glioma cell lines: The proteasome inhibitor, bortezomib, potentiates axitinib-induced cytotoxicity in a p21(Waf/Cip1) dependent manner ,Morelli, MB;Amantini, C;Nabissi, M;Cardinali, C;Santoni, M;Bernardini, G;Santoni, A;Santoni, G;, Oncotarget 2017 ,PubMed ID 27926485 [GAPDH]
Heparanase Overexpression Reduces Hepcidin Expression, Affects Iron Homeostasis and Alters the Response to Inflammation ,Asperti, M;Stuemler, T;Poli, M;Gryzik, M;Lifshitz, L;Meyron-Holtz, EG;Vlodavsky, I;Arosio, P;, PLoS ONE 2016 ,PubMed ID 27711215 [GAPDH]
Alcohol Dehydrogenases and Acetaldehyde Dehydrogenases are Beneficial for Decidual Stromal Cells to Resist the Damage from Alcohol ,Li, Z;Li, M;Li, S;Fu, Y;Yang, Z;, Alcohol and Alcoholism 2016 ,PubMed ID 28182209 [GAPDH]
Renal Protective Effects of 17ß-Estradiol on Mice with Acute Aristolochic Acid Nephropathy ,Shi, M;Ma, L;Zhou, L;Fu, P;, Molecules 2016 ,PubMed ID 27763560 [GAPDH]
Data on the inhibition of RNase inhibitor activity by a monoclonal antibody as assessed by microfluidics-based RNA electrophoresis ,Wang, X;Teferedegne, B;Shatzkes, K;Tu, W;Murata, H;, Data Brief 2016 ,PubMed ID 27699193 [GAPDH]
Grainyhead-like 2 inhibits the coactivator p300, suppressing tubulogenesis and the epithelial-mesenchymal transition ,Pifer, PM;Farris, JC;Thomas, AL;Stoilov, P;Denvir, J;Smith, DM;Frisch, SM;, Mol. Biol. Cell 2016 ,PubMed ID 27251061 [APDH]
Cytosolic localization of Fox proteins in motor neurons of G93A SOD1 mice ,Ma, X;Turnbull, PC;Crapper, EP;Wang, H;Drannik, A;Jiang, F;Xia, S;Turnbull, J;, Histochem. Cell Biol. Jan 2016 ,PubMed ID 26724814 [GAPDH]
TRAF3IP3, a novel autophagy-upregulated gene, is involved in MZ B lymphocyte development and survival ,Peng, S;Wang, K;Gu, Y;Chen, Y;Nan, X;Xing, J;Cui, Q;Chen, Y;Ge, Q;Zhao, H;, Clin. Exp. Immunol. May 2015 ,PubMed ID 26011558 [GAPDH]
Aberrant over-expression of TRPM7 ion channels in pancreatic cancer: required for cancer cell invasion and implicated in tumor growth and metastasis ,Yee, NS;Kazi, AA;Li, Q;Yang, Z;Berg, A;Yee, RK;, Biol Open Mar 2015 ,PubMed ID 25770184 [GAPDH]
Metabolic Reprogramming of Cancer-Associated Fibroblasts by IDH3a Downregulation ,Zhang, D;Wang, Y;Shi, Z;Liu, J;Sun, P;Hou, X;Zhang, J;Zhao, S;Zhou, BP;Mi, J;, Cell Rep Mar 2015 ,PubMed ID 25732824 [GAPDH]
Toxicity of aflatoxin B1 towards the vitamin D receptor (VDR) ,Costanzo, P;Santini, A;Fattore, L;Novellino, E;Ritieni, A;, Food Chem. Toxicol. Dec 2014 ,PubMed ID 25483621 [GAPDH]
Novel APP K724M mutation causes Chinese EOFAD and increases amyloid-ß42/40 ratio ,Peng, XL;Hou, L;Xu, SH;Hua, Y;Zhou, SJ;Zhang, Y;, Neurobiology of Aging Jun 2014 ,PubMed ID 25018108 [GAPDH]
A novel protein is lower expressed in renal cell carcinoma ,Guan, R;Xu, Y;Lei, H;Gao, Z;Xin, Z;Guo, Y;, Int J Mol Sci May 2014 ,PubMed ID 24786097 [GAPDH]
Aberrantly Over-Expressed TRPM8 Channels in Pancreatic Adenocarcinoma: Correlation with Tumor Size/Stage and Requirement for Cancer Cells Invasion ,Yee, NS;Li, Q;Kazi, AA;Yang, Z;Berg, A;Yee, RK;, Cells May 2014 ,PubMed ID 24861976 [GAPDH]
Biochemical and functional interaction between ZNF224 and ZNF255, two members of the Krüppel-like zinc-finger protein family and WT1 protein isoforms ,Francesca Florio, Elena Cesaro, Giorgia Montano, Paola Izzo, Colin Miles, and Paola Costanzo, Hum. Mol. Genet., Sep 2010; 19: 3544 – 3556 ,PubMed ID 20591825 [GAPDH]