【Product Description】
DMEM, High Glucose, with Sodium Pyruvate, with L-Glutamine is one of the most widely used modification of Eagle's medium.
DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs glucose per litre and was originally used to culture embryonic mouse cells.
DMEM high glucose is a further modification of original DMEM and contains 4500mgs glucose per litre. The additional glucose has proved to be useful in cultivating various other cell lines including primary cultures of mouse and chicken cells as well as various normal and transformed cell lines.
Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
【Composition】
| Ingredients | Mg/L |
| INORGANIC SALTS | |
| Calcium chloride dihydrate | 265.000 |
| Ferric nitrate nonahydrate | 0.100 |
|
Magnesium sulphate anhydrous |
97.720 |
| Potassium chloride | 400.000 |
| Sodium chloride | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 109.000 |
| AMINO ACIDS | |
| Glycine | 30.000 |
|
L-Arginine hydrochloride |
84.000 |
|
L-Cystine dihydrochloride |
62.570 |
| L-Glutamine | 584.000 |
| L-Histidine hydrochloride monohydrate | 42.000 |
| L-Isoleucine | 105.000 |
| L-Leucine | 105.000 |
| L-Lysine hydrochloride | 146.000 |
| L-Methionine | 30.000 |
| L-Phenylalanine | 66.000 |
| L-Serine | 42.000 |
| L-Threonine | 95.000 |
| L-Tryptophan | 16.000 |
| L-Tyrosine Disodium Salt | 103.790 |
| L-Valine | 94.000 |
| VITAMINS | |
| Choline chloride | 4.000 |
| D-Ca Pantothenate | 4.000 |
| Folic acid | 4.000 |
| Nicotinamide | 4.000 |
| Pyridoxal hydrochloride | 4.000 |
| Riboflavin | 0.400 |
| Thiamine hydrochloride | 4.000 |
| i-Inositol | 7.200 |
| OTHERS | |
|
D-Glucose |
4500.000 |
| Phenol red Sodium Salt | 15.900 |
| Sodium pyruvate | 110.000 |
| Sodium bicarbonate | 3700.000 |
【Storage and Stability】
DMEM , High Glucose, with Sodium Pyruvate, with L-Glutamine should be kept 2-8°C. The product is light -sensitive and therefore should not be left in the light. When stored in the dark under ideal conditions, the product is stable until the expiry date.
As with any other liquid media formulations, deterioration of liquid media may be recognized by any of the following characteristics, among others including:
(a). Color Change;
(b). Presence of clumping/flocculent debris/ granulation/ particulates precipitates or sediments;
(c). Insolubility;
(d). And/or decrease in expected performance parameters.
Any material described above should not be used and therefore discarded.
【Procedure】
1.Take a bottle from the defined storage conditions at 2-8°C and read the label.
2.Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.
3.Using aseptic/sterile technique under a laminar-flow culture hood, work according to established protocols.
4.Antibiotics may be added if desired.
【Quality Control】
DMEM , High Glucose, with Sodium Pyruvate, with L-Glutamine is tested for sterility, pH, osmolality and endotoxin concentrations. In addition, each batch is tested for cell growth using A549.
【Precaution and Disclaimer】
For research use only, not for clinical diagnosis and treatment.