德国MN NucleoBond Xtra Midi 超快速转染级质粒中提试剂盒 740410.50
NucleoBond(R) Xtra Midi / Maxi
德国MN NucleoBond Xtra Midi 超快速转染级质粒中提试剂盒
货号：740410.50
规格：50 preps
品牌：德国MN
从大肠杆菌中提取高拷贝/低拷贝质粒。新一代阴离子交换柱-短时间，高产量，转染用质粒。
特点：
节约60%的时间，增加100%的产量
-
新一代阴离子交换–超快，高产量的转染级别的质粒DNA
-
优化设计的柱子：制备时间约为30 分钟(Midi prep)；35 分钟(Maxi prep)
-
包含柱过滤器：高过虑流速，清除裂解液中不溶物的同时可向柱子加样
-
改进的硅材料：标准的产量250μg-1000μg
-
专li的阴离子交换技术得到转染级别的质粒DNA
-
NucleoBond(R)Xtra Plus kits 包含NucleoBond(R) Finalizers；加速了DNA的沉淀
2nd generation of anion exchanger for fast purification of plasmid DNA
| Selling unit | 10 Prep(s), 50 Prep(s), 100 Prep(s) |
| Application | Transfection-grade plasmid DNA isolation |
| Target | Plasmid DNA |
| CE certified | No, research use only |
| Technology | Anion exchange chromatography |
| Brand | NucleoBond |
| Format | Midi prep |
| Handling | Gravity flow |
| Lysate clarification | Column filter |
| Automated use | Yes, can be automated on the Andrew+ the pipetting robot |
| Sample material | Bacteria, E. coli |
| Sample amount | < 200 mL (high copy), < 400 mL (low copy) |
| Vector size | < 300 kbp |
| Typical yield | 500 µg |
| Theoretical binding capacity | 800 µg |
| Typical purity A260/A280 | 1.8−1.95 |
| Endotoxin level | 1−10 EU/µg DNA |
| Isopropanol precipitation | Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi |
| Preparation time | 70 min/prep |
| Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 39 Month(s) |
| Hazardous material | Yes |
产品概览
|
NucleoBond(R) XtraMidi |
NucleoBond(R) Xtra Maxi |
|||
|
技术 |
阴离子交换色谱法 |
|||
|
产品形式 |
Midi gravity-flow columns |
Maxi gravity-flow columns |
||
|
裂解液澄清 |
柱过滤器 |
柱过滤器 |
||
|
样品材料 |
< 200 mL (高拷贝) < 400 mL (低拷贝) |
< 600 mL (高拷贝) < 1 200 mL (低拷贝) |
||
|
载体大小 |
< 300 kbp |
< 300 kbp |
||
|
产量 |
250 μg |
1000 μg |
||
|
A260/A280 |
1.80 – 1.95 |
1.80 – 1.95 |
||
|
异丙醇沉淀 |
Xtra Midi |
Xtra Midi Plus |
XtraMaxi |
XtraMaxi Plus |
|
Centrifugation |
NucleoBond(R) Finalizer |
Centrifugation |
NucleoBond(R)Finalizer Large |
|
|
制备时间 |
70min/4preps |
30min/4preps |
80min/4preps |
35min/4preps |