Thermo CellTracker™ CM-DiI 活细胞示踪剂

订购信息：【原装产品，现货提供】

基本介绍：

CM-DiI（活细胞的多代示踪、固定细胞长期示踪）

CellTracker CM-DiI为DiI的衍生物，在DiI中加入了具有中度巯基反应特性的氯甲基取代物，后者能与细胞内含巯基的肽和蛋白结合，使得CM-DiI能够抵抗醛类的固定作用。CM-DiI在细胞固定、破膜及石蜡包埋的整个过程中均能很好的保留在细胞内，因此，与传统细胞膜探针DiI相比较，更适于固定后标本的长期示踪。其红色/绿色的光谱特征，使其可与绿色探针或者绿色荧光蛋白如GFP一起用于双标实验。

产品特性

‍1）CAS NO：180854-97-1‍

2）化学名：3H-Indolium, 5-[[[4-(chloromethyl)benzoyl]amino]methyl]-2-[3-(1,3-dihydro-3,3-dimethyl-1- octadecyl-2H-indol-2-ylidene)-1-propenyl]-3,3-dimethyl-1-octadecyl-, chloride

3）分子式：C68H105Cl2N3O

4）分子量：1051.5g/mol

5）纯度：≥95%

6）外观：红色固体

7）Ex/Em：553/570 nm（甲醇）

8）推荐滤光器：Omega-XF32, Chroma-31002

9）溶解性：溶于DMSO，乙醇，DMF

10）化学结构图：

染色示例

Fig 1.Brain of a zebrafish embryo. CellTracker CM-DiI. A 30 µm–thick section of a zebrafish embryo stained with CellTracker CM-DiI (C7000, C7001) prior to immunohistochemical analysis. A one-day-old zebrafish embryo was immobilized and impaled in the hindbrain with a microelectrode filled with 1 mg/mL CellTracker CM-DiI in 95% ethanol. One day later, the brain was fixed, embedded, frozen in liquid nitrogen and sectioned on a cryostat. After blocking with 0.1% Triton X-100 in PBS containing 2% BSA and 2% normal goat serum, the 30 µm–thick section was incubated with primary anti-glial antibody in conjunction with fluorescein goat anti–mouse IgG antibody. This section was then viewed sequentially through optical filter sets appropriate for rhodamine and fluorescein, and the resulting images were superimposed. The image was contributed by William Trevarrow, Beckman Institute, California Institute of Technology.

细胞膜荧光探针系列产品：

1. 常见细胞膜探针和检测光谱