Nugen 0340-32 人类FFPE RNA文库构建试剂盒在Illumina仪器平台上测序的链特异性RNA-Seq文库构建提供了整体解决方案

Ovation人类FFPE RNA-Seq多重文库构建试剂盒是为在Illumina仪器平台上测序的链特异性RNA-Seq文库构建提供了整体解决方案。 该试剂盒结合了高效灵活的工作流程,适用于大范围的总RNA输入(10-250 ng),并采用AnyDeplete技术有效去除人类rRNA序列的垃圾背景序列。

Ovation人类FFPE RNA-Seq多重文库构建试剂盒使用从人类组织或细胞系获得的纳克级总RNA,为链特异性RNA-Seq文库构建提供了端到端的解决方案。该工作流程同样适用于新鲜冷冻样品(低至10 ng)的总RNA或FFPE样品(低至100 ng)的保存RNA。从总RNA输入到完整构建的文库的整合工作流程具有很高的可重复性,可以在大约7小时内完成。 NuGEN的依赖插入的AnyDeplete技术可有针对性地消除不需要的转录本,并显着减少了源自人类rRNA(12S,16S,18S和28S基因)的测序数据,从而更有效地利用了测序通量。该试剂盒均提供八个*的条形码接头,以使多重测序能够进一步优化功效。

简化的工作流程包括使用随机和多聚(T)引物的混合物反转录生成双链cDNA,双链cDNA打断,末端修复以生成平末端,接头进行连接,通过核苷酸类似物靶向降解的链选择,AnyDeplete技术介导的接头裂解和PCR扩增以产生终文库。 整个工作流程,包括片段化,都可在短短7个小时内完成,并生成可用于在Illumina测序平台上进行单端或双端测序的簇形成的DNA文库。

NuGEN系列产品

NuGEN #0360-08 Celero DNA-Seq , 8rxn
NuGEN #0360-24 Celero DNA-Seq , 24rxn
NuGEN #0357-08 Trio RNA-Seq , 8rxn
NuGEN #0357-32 Trio RNA-Seq , 32rxn
NuGEN #0357-96 Trio RNA-Seq , 96rxn
NuGEN #0508-08 Universal Plus mRNA-Seq , 8rxn
NuGEN #0508-32 Universal Plus mRNA-Seq , 32rxn
NuGEN #0508-96 Universal Plus mRNA-Seq , 96rxn
NuGEN #0508-A01 Universal Plus mRNA-Seq , 96rxn
NuGEN #0520-24 Universal Plus mRNA-Seq with NuQuant , 24rxn
NuGEN #0520-A01 Universal Plus mRNA-Seq with NuQuant , 96rxn
NuGEN #0500-32 Ovation® SoLo RNA-Seq System, Human , 32rxn
NuGEN #0500-96 Ovation® SoLo RNA-Seq System, Human , 96rxn
NuGEN #0501-32 Ovation® SoLo RNA-Seq System, Mouse , 32rxn
NuGEN #0501-96 Ovation® SoLo RNA-Seq System, Mouse , 96rxn
NuGEN #7102-08 Ovation® RNA-Seq System V2 , 8rxn
NuGEN #7102-32 Ovation® RNA-Seq System V2 , 32rxn
NuGEN #7102-A01 Ovation® RNA-Seq System V2 , 96rxn
NuGEN #7150-08 Ovation® RNA-Seq FFPE System , 8rxn
NuGEN #0335-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 1-8 , 32rxn
NuGEN #0336-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 9-16 , 32rxn
NuGEN #9113-A01 Ovation® Ultralow Methyl-Seq System , 96rxn
NuGEN #0535-32 Ovation Ultralow Methy-Seq DR Multiplex System 1-8, with TrueMethyl® oxBS , 32rxn
NuGEN #0536-32 Ovation Ultralow Methy-Seq DR Multiplex System 9-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0553-32 Ovation RRBS Methyl-Seq System 1-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0414-32 TrueMethyl® oxBS Module , 32rxn
NuGEN #3302-12 Ovation® Pico WTA System V2 , 12rxn
NuGEN #3302-60 Ovation® Pico WTA System V2 , 60rxn
NuGEN #3302-A01 Ovation® Pico WTA System V2 , 96rxn
NuGEN #3403-12 Ovation® FFPE WTA System , 12rxn
NuGEN #3403-60 Ovation® FFPE WTA System , 60rxn
NuGEN #3403-A01 Ovation® FFPE WTA System , 96rxn
NuGEN #3100-12 Ovation® RNA Amplification System V2 , 12rxn
NuGEN #3100-60 Ovation® RNA Amplification System V2 , 60rxn
NuGEN #3100-A01 Ovation® RNA Amplification System V2 , 96rxn
NuGEN #0344-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-08 Ovation® Ultralow System V2 , 8rxn
NuGEN #0344NB-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-A01 Ovation® Ultralow System V2 , 96rxn