Lumiprobe 磺酸基-Cy5-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine5 NHS ester
水溶Cyanine 5琥珀酰亚胺酯(succinimidyl ester(SE)),类似于Cy5® NHS ester,不需要有机溶剂即在水相中即可对各种带氨基分子进行标记。因此该产品尤其适用于对那些在有机溶剂中易变性的蛋白和低溶解度蛋白的标记。Sulfo-Cyanine 5是Cy5®的衍生物,与很多仪器如读板机(plate readers),显微镜和成像设备等相兼容。是最受欢迎的荧光染料之一。
该染料是高度亲水的、水溶的。非磺化的衍生物:Cyanine 5 NHS ester。可以在所有的应用中替代Cy5®,Alexa Fluor 647和DyLight 649。
Lumiprobe 磺酸基-Cy5-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine5 NHS ester 一般特性
外观: | dark blue powder |
分子量: | 761.84 |
分子式: | C36H40N3NaO10S2 |
溶解性: | very good in water, good in DMF and DMSO |
质量控制: | NMR 1H (95%) and 13C, TLC, functional testing |
储存条件: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
光谱特性
最大激发波长, nm: | 646 |
在最大激发波长下的消光系数, Lmol-1cm-1: | 271000 |
最大发生波长, nm: | 662 |
荧光量子产率: | 0.2 |
CF260: | 0.13 |
CF280: | 0.13 |
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