Megazyme L-CONA 刀豆球蛋白A Concanavalin A (Con A)
Megazyme品牌介绍：
爱尔兰Megazyme是生产分析试剂、酶和食品酶法试剂盒的技术性企业，开发饮料、谷类食品、乳制品、食品、饲料、发酵、生物燃料和葡萄酒行业检测试剂盒和试剂。从公司成立之初的检测试剂盒试剂盒生产，到现在新的领域，碳水化合物的和谷物酶相关行业发展已成为公认的检测领域领导者之一。
Megazyme L-CONA 刀豆球蛋白A Concanavalin A (Con A)
中文名称：刀豆球蛋白A
英文名：Concanavalin A (Con A)
货号：ML-CONA-1000MG
规格：1 g
High purity Concanavalin A (Con A) lectin has a highly specific carbohydrate binding affinity, for research and in vitro diagnostic analysis.
Affinity purified, lyophilised powder. Con A is not blood group specific, has an affinity for terminal α-D-mannose and α-D-glucose residues and requires the presence of Ca2+ and Mn2+ for activity.
高纯度刀豆蛋白A(ConA)凝集素具有高度特异性的碳水化合物结合亲和力，用于研究和离体 诊断分析
亲和纯化冻干粉末。Con A不具有血型特异性，对末端α-D-甘露糖和α-D-葡萄糖残基具有亲和力，需要钙的存在。2+锰2+为了活动。
DESCRIPTION
(1) FORM
Affinity purified, off-white lyophilised powder.
(2) BIOCHEMICAL / PHYSIOLOGICAL PROPERTIES
Con A is not blood group specific, has an affinity for terminal α-D-mannose and α-D-glucose residues and requires the presence of Ca2+ and Mn2+ for activity. Con A exists in dimeric (pH < 5.6), tetrameric (pH between 5.6 and 7.0) and aggregate (pH > 7.0) forms. An active dimer above pH 5.6 can be generated by succinylation. Con A exhibits mitogenic activity which is dependent upon its degree of aggregation.
(3) PROPERTIES
Activity: 20 μg/mL
Electrophoretic purity: Electrophoresis was preformed using a 14% acrylamide gel.
UV absorbance: In 100 mM NaCl; λmax = 275.8; E1% = 13.7.
Solubility: Slightly hazy, colourless solution at 10 mg/mL in water.
Activity note: Activity is determined using a twofold serial dilution of 1 mg/mL solution of
Con A in PBS* (8.0 g NaCl, 0.3 g KCl, 0.2 g KH2PO4/L; pH 7.2) containing 1
mM Ca2+ and 1 mM Mn2+. The activity is expressed as the lowest concentra-
tion to give agglutination of a 2% suspension of human red blood cells (type O)
in PBS* after 1 hr incubation at room temperature.
(4) STORAGE / STABILITY / SAFETY
Storage temperature: -20ºC
Shelf life: > 3 years at -20ºC
Safety statement: 22-24 / 25
Reference 参考文献：
Entlicher, G.J., Kostir, V. and Kocourek, J. (1971) Studies on phytohemmagglutinins.
8. Isoelectric point and multiplicity of purified concanavalin A. Biochim Biophys Acta 236(3): 795-7