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Carbo-Free Blocking Solution (10x Concentrate)
Description
The Carbo-Free Blocking Solution is a protein-based agent used as a general blocking or diluent solution. It is ideal for applications using lectins in which glycoprotein contamination could generate background staining or false positive results.
Features:
- Essentially free of glycoproteins, unlike other common protein-containing blocking agents like serum, nonfat dry milk, or casein
- Supplied as 125 ml of 10x concentrate
Specifications
| Unit Size | 125 ml |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
| Recommended Storage | 2-8 °C |
| Recommended Usage | Prior to use, 10x Carbo-Free Blocking Solution should be diluted to 1x with distilled or deionized water. For prolonged storage of the 1x solution, sodium azide should be added to give a final concentration of 0.08% (w/v) and the diluted solution refrigerated. Carbo-Free Blocking Solution does not contain detergent. In some applications, blocking may be enhanced by addition of Tween™ 20 to a final concentration of 0.05% to 0.1%, in the working solution. |
| Blocking Action | Non-Specific Protein Blocking |
| Format | Concentrate |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
10x Casein Solution Catalog #: SP-5020-250
Animal-Free Blocker®, 5X Concentrate Catalog #: SP-5030-250
Bovine Serum Albumin (BSA), Immunohistochemical Grade Catalog #: SP-5050-500
Animal-Free Blocker® and Diluent, R.T.U. Catalog #: SP-5035-100
Citations
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A suggested protocol for detection of glycoconjugates using lectins/agglutinins is available under the "PROTOCOLS" tab.