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Rabbit Anti-Goat IgG Antibody (H+L), AMCA
Description
AMCA Rabbit Anti-Goat IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.
Features:
- Suitable for use with goat, sheep, and bovine IgG primary antibodies
- Recognizes both heavy and light chains (H+L)
- Optimally labeled with AMCA to provide the brightest label for fluorescence microscopy
- Supplied in solution
- Excitation: 350 nm
- Emission: 450 nm
- Color: Blue
Specifications
| Unit Size | 1.5 mg |
|---|---|
| Applications | Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation |
| Concentration | 1.5 mg active conjugate/ml |
| Recommended Storage | 2-8 °C |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. |
| Maximum Emission | 448-454 nm. |
| Maximum Excitation | 345-355 nm |
| Recommended Usage | The recommended concentration range for use is 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue. |
| Target Species | Goat |
| Conjugate | AMCA |
| Color of Fluorescence | Blue |
| Host Species | Rabbit |
| Format | Concentrate |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
Rabbit Anti-Goat IgG Antibody (H+L), Biotinylated Catalog #: BA-5000-1.5
Horse Anti-Goat IgG Antibody (H+L), DyLight® 488 Catalog #: DI-3088-1.5
Horse Anti-Goat IgG Antibody (H+L), DyLight® 594 Catalog #: DI-3094-1.5
VECTASHIELD® Antifade Mounting Medium Catalog #: H-1000-10
VECTASHIELD® HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
Rabbit Anti-Goat IgG Antibody (H+L), Unconjugated Catalog #: AI-5000-1.5
Technical Information
AMCA (7-amino-4-methylcoumarin-3-acetic acid) labeled Rabbit Anti-Goat IgG Antibody excites in the near ultraviolet (350 nm) and emits a visible bright blue fluorescence at about 450 nm. This antibody has been optimally labeled with AMCA to provide the brightest label for fluorescence microscopy.
The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker® solution (Cat. No. SP-5030) is recommended.