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Phaseolus Vulgaris Leucoagglutinin (PHA-L), Biotinylated
Description
Phaseolus vulgaris agglutinin is the name ascribed to a family of lectins, each of which consists of four subunits. There are two different types of subunits. One appears to be involved primarily in red cell agglutination and has been designated the “E” subunit (for erythroagglutinin). The other type is involved in lymphocyte agglutination and mitogenic activity and has been termed the “L” subunit (for leucoagglutinin). These subunits combine to produce five isolectins. PHA-L, with four “L” type subunits, does not agglutinate red cell but is a potent mitogen.
Biotinylated PHA-L has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
Specifications
| Unit Size | 2 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
| Recommended Usage | For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer. |
| Recommended Storage | 2-8 °C |
| Solution | 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2 |
| Concentration | 2 mg active conjugate/ml |
| Conjugate | Biotinylated |
| Sugar Specificity | Galactose, Complex Structures |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
Related Products
Phaseolus Vulgaris Leucoagglutinin (PHA-L), Rhodamine Catalog #: RL-1112-2
Phaseolus Vulgaris Leucoagglutinin (PHA-L), Unconjugated Catalog #: L-1110-5
Phaseolus Vulgaris Leucoagglutinin (PHA-L), Fluorescein Catalog #: FL-1111-2
Citations
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This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Elution: 100 mM acetic acid