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NxGen phi29 DNA Polymerase

NxGen Phi29 DNA Polymerase is a highly processive and high-fidelity DNA Polymerase that enables accurate amplification of DNA from limited or degraded samples. 

Product Details

The NxGen Phi29 DNA Polymerase sources its enzyme from a recombinant E. coli strain carrying the phi29 DNA polymerase gene from bacteriophage phi29.

1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30 °C.

The enzyme storage buffer consists of 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.5% Tween-20, 0.5% NP-40, 50% glycerol, pH 7.4 @ 25 °C and the 10X phi29 DNA Polymerase Buffer is composed of 500 mM Tris-HCl, 100 mM (NH4)2SO4, 40 mM Dithiothreitol, 100 mM MgCl2, pH 7.5 @ 25 °C.

Key features

  • Powerful strand displacement activity, making it ideal for challenging samples or damaged DNA

  • Highly processive, allowing for amplification of up to 70,000 base insertions per binding event

  • High-fidelity 3'->5' proofreading exonuclease function for accurate DNA replication with low error rates

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