Wako 019-19741 Anti Iba1, Rabbit

发表于由

Wako 和光纯药 Anti Iba1, Rabbit (for Immunocytochemistry)

Iba1抗体，兔源（免疫細胞化学用）

品牌 产品编号 产品名称 包装
WAKO 019-19741 Anti Iba1, Rabbit (for Immunocytochemistry) 50ug

钙离子作为信号转导通道中的第一信使，在所有细胞包括中枢神经细胞的生命活动中起重要作用。钙离子与不同的钙结合蛋白结合后进行级联反应。Iba1是能与巨噬细胞和小胶质细胞发生特异性结合的钙结合蛋白，当这些细胞激活后，Iba1的表达量上调，因此Iba1通常作为鉴定小胶质细胞的标记物使用。近来研究发现，小胶质细胞除了提供营养、保护神经的作用外，还被证明可产生NO、TNF-a、IL-1β等物质，因此将小胶质细胞定义为中枢神经系统的巨噬细胞样吞噬细胞，具有重要的免疫细胞作用。当病原菌、病毒入侵中枢神经系统时会起免疫监御的作用。近来研究发现，该细胞可能与神经退行性疾病的发生和发展有关。本产品是可与小胶质细胞发生特异性反应的兔多抗，配合对星形胶质细胞有特异性的GFAP单抗，可进行双染色。

抗原：合成肽（Iba1 的C末端序列）

外观：TBS溶液（1mg/ml）

提纯：抗原亲和提纯

特异性：对小胶质细胞、巨噬细胞特异反应。不会与神经元、星形胶质细胞发生交叉反应。能与人、小鼠、大鼠Iba1反应。

用法：IBA1抗体，兔源（免疫細胞化学用）适合免疫細胞化学。只需1~2μg/ml即可进行实验。

  • Anti Iba1, Rabbit

    019-19741(For Immunocytochemistry) & 016-20001 (for Western Blotting)

    Iba1: ionized calcium binding adapter molecule 1 小胶质细胞/巨噬细胞特异性蛋白抗体

    钙离子是已知的所有细胞，包括中枢神经系统（CNS）细胞中最重要的信号调解人之一。钙离子通过与各种钙结合蛋白结合发挥信号活动，其中许多钙结合蛋白被划分成一个大的蛋白家族，EF手性蛋白家族。Iba1是一个17 kDa的EF手性蛋白，在巨噬细胞和小胶质细胞中表达，并在这些细胞的活化过程中表达升高。
    本抗体以合成的的Iba1羧基末端序列为抗原，该序列是人，大鼠和小鼠Iba1蛋白质保守序列。本抗体（免疫组化用）特异性与小胶质细胞/巨噬细胞反应，免疫双重染色与单克隆抗体结合，可与GFAP单克隆抗体（(胶质纤维酸性蛋白，Glial Fibrillary Acidic Protein）联合使用对脑组织和细胞培养物进行双重染色。

    特点

    S特异性检测小胶质细胞与/巨噬细胞，不与神经细胞与星形胶质细胞发生交叉反应。可与人，大鼠，小鼠的Iba1反应

    货号 品名 包装 用途 工作浓度
    019-19741 Anti Iba1 polyclonal antibody, Rabbit, for Immunocytochemistry 50ug(100ul) IH 1-2ug/ml
    016-20001 Anti Iba1 polyclonal antibody, Rabbit, for Western Blotting 50ug(100ul) WB 0.5-1ug/ml

    应用

    石蜡切片应用方法：
    1. 固定
    2. 用0.01M PBS缓冲液清洗二次
    3. 使用含有0.3% 过氧化氢的甲醇孵育30分钟
    4. 用0.01M PBS缓冲液清洗三次
    5. 使用封闭液（含有1.5%的山羊血清和1%BSA的0.001M PBS缓冲液）室温孵育2小时
    6. 使用含有Anti Iba1 polyclonal antibody的封闭液4℃孵育过夜
    7. 用0.01M PBS缓冲液清洗三次
    8. 使用含有生物素标记的抗兔IgG（1：200）的封闭液室温孵育1小时
    9. 用0.01M PBS缓冲液清洗三次
    10. 使用Elite ABC试剂（含有试剂A1：50，试剂B1：50的0.01M PBS溶液）
    11. 用0.01M PBS缓冲液清洗三次

    12. 过氧化物酶溶液（含有0.01% 过氧化氢和0.05% DAB的0.05M Tris溶液）

    英文参考：

    Summary

    This product is for research use only. Do not administer it to human.

    Antibodies against Macrophage/Microglia-specific Protein Iba1

    Calcium ions are known to be one of the most important signal mediators in all cells including central nervous system (CNS) cells. Calcium ions exert their signaling activity through association with various calcium binding proteins, many of which are classified into a large protein family, the EF hand protein family. Iba1 is a 17-kDa EF hand protein that is specifically expressed in macrophages/ microglia and is upregulated during the activation of these cells.Wako has launched rabbit polyclonal antibodies were raised against a synthetic peptide corresponding to the Iba1 carboxy-terminal sequence, which was conserved among human, rat and mouse Iba1 protein sequences. These antibodies are specifically reactive to microglia/ macrophages, are appropriate for immuno-double staining of brain tissues and cell culture in combination with monoclonal antibody to GFAP, which specifically reacts to astrocyte.

    Rabbit Anti Iba1 antibody is raised a synthetic peptide corresponding to C-terminus of Iba1.

    Preparation: Purified by the antigen affinity chromatography from rabbit antisera and prepared in TBS solution. Contains no preservatives and stabilizers.

    Specificity: Specific to microglia and macrophage, but not cross-reactive with neuron and astrocyte. Reactive with human, mouse and rat Iba1.

    Working Concentration: Immunocytochemistry 1-2 micro-g/mL

    Storage:Store at -20 degrees C. After opening, aliquot contents and freeze at -20 degrees C.

    Package Size: 50 ug (100 uL) (ug: micro-g; uL: micro-L)

    References:

    Imai, Y., Ibata, I., Ito, D., Ohsawa, K. and Kohsaka, S.: Biochem. Biophys. Res. Commun., 224, 855 (1996).

    Ito, D., Imai, Y., Ohsawa, K, Nakajima, K, Fukuuchi, Y. and Kohsaka, S.: Brain Res. Mol. Brain Res., 57, 1 (1998).

    Ohsawa, K., Imai, Y., Kanazawa, H., Sasaki, Y. and Kohsaka, S.: J. Cell Sci., 113, 3073 (2000).

    Sasaki, Y., Ohsawa, K., Kanazawa, H., Kohsaka, S. and Imai, Y.: Biochem. Biophys. Res. Commun., 286, 292 (2001).

    Kanazawa, H., Ohsawa, K., Sasaki, Y., Kohsaka, S. and Imai, Y.: J. Biol. Chem., 277, 20026(2002).

    Wako catalog #016-20001 Anti Iba1 polyclonal antibody, Rabbit, for Western Blotting (50 micrograms (100 microlitters))Working Concentration: Westernblot 0.5 – 1 micrograms/mL

    [Recommended preparations]

    Frozen mouse brain tissue or cultured cells should be perfusion-fixed with 4% paraformaldehyde-PBS. Then the tissue section is prepared. After those preparation, the tissue section and cultured cells are immunostained with anti Iba1 antibody.

    其他相关产品：

    相关产品有：

    产品编号 产品名称 包装
    016-20001 Anti Iba1, Rabbit (for Western Blotting) 50ug
    301-08781 Anti Killifish Vitellogenin Monoclonal Antibody (3C1) 100ug
    016-11451 Anti Leucine-Enkephalin, Rabbit for Immunochemistry 100uL
    308-08811 Anti Metallothionain, Monoclonal Antibody (clone No. 1A12) 100ug
    015-19721 Anti 5-Methylcytosin, Monoclonal Antibody for Immunochemistry 100uL
    013-13781 Anti Mouse 2.5S-Nerve Growth Factor(Rabbit), Affinity purified IgG fraction 1mL
    014-22023 Anti Mouse Ago2, Monoclonal Antibody 50ul
    018-22021 Anti Mouse Ago2, Monoclonal Antibody 100ul
    016-16451 Anti Mouse CD38, Rat Monoclonal Antibody for Immunochemistry 200ug
    016-15351 Anti Mouse CD44, Rat Monoclonal Antibody(clone:KM201) for Immunochemistry 500ug
    012-15951 Anti Mouse CDK2, Monoclonal Antibody 250uL
    014-18071 Anti Mouse Cox-2,Rabbit，for Immunochemistry 100uL
    010-14031 Anti Mouse IgG (H+L), Goat, IgG Whole, Biotin Conjugated, affinity purified 1mg
    018-17871 Anti Mouse IgG(H+L), Goat, R-Phycoerythrin Conjugated 250ul

     

    • 货号   品名     规格  Cas

      012-01965   Alminium Oxide   500g     –

      012-03305   Ammonium Dihydrogenphosphate  500g     7722-76-1

      012-08643   Amino Acids Mixture Standard Solution, Type B 1mL×5A –

      013-08391   Amino Acids Mixture Standard Solution, Type H 5mL      –

      015-14461   Amino Acids Mixture Standard Solution, Type AN-2  5mL      –

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      015-25191   Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64)      50ul      –

      011-14463   Amino Acids Mixture Standard Solution, Type AN-2  1mL×5A –

      011-19365   2,2′-Azobis[2-(2-imidazolin-2-yl)propane]

      Dihydrochloride   500g     27776-21-2

      016-08641   Amino Acids Mixture Standard Solution, Type B 5mL      –

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      016-20001   Anti Iba1, Rabbit (for Western Blotting)     50μg     –

      016-23281   Anti Rat P2X4, Monoclonal Antibody     50ug     –

      017-19362   2,2′-Azobis[2-(2-imidazolin-2-yl)propane] Dihydrochloride    25g 27776-21-2

      018-08721   Agar, Powder     100g     9002-18-0

      018-22021   Anti Mouse Ago2, Monoclonal Antibody 100μL    –

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货
      019-03435   Ammonium sulfate    500g     7783-20-2

      019-19741   Anti Iba1, Rabbit (for Immunocytochemistry)    50μg     –

      019-22291   Anti Olfactory Marker Protein, Goat      100μL    –

      021-02195   Boric acid     500g     10043-35-3

      021-16201   BES-H2O2(Cell-impermeant)   1mg      –

      031-17601   Collagenase Type I    100mg   9001-12-1

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      Wako   019-19741,小胶质细胞/巨噬细胞特异性抗体

      032-08385   Citric Acid Monohydrate   500g     5949-29-1

      032-15372   Casein Phosphopeptide    25g –

      034-06363   N-Caprylic Acid   25mL    124-07-2

      034-16111   Cefotaxime Sodium Salt   1g   64485-93-4

      035-04595   Creatinine    500g     60-27-5

      035-17604   Collagenase Type I    1g   9001-12-1

      038-23221   α-Casein, from Bovine Milk, Dephosphorylated  1mg      –

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      039-01335   Carboxymethyl Cellulose Sodium Salt   500g     9004-32-4

      021-17801   BES-So-AM 1mg      936356-51-3

      022-07985   γ-Butyrolactone  500mL   96-48-0

      025-03195   Buffer Solution Standard (Phosphate pH Standard Equimolal Solution)

      pH6.86 (25 degrees C)    500mL   –

      047-18863   Dexamethasone 100mg   50-02-2

      049-18801   4′,6-Diamidino-2-phenylindole Dihydrochloride n-Hydrate      10mg      28718-90-3

      058-07681   EasySeparator    1set      –

      Wako   019-19741,小胶质细胞/巨噬细胞特异性抗体

      025-03195   Buffer Solution Standard (Phosphate pH Standard Equimolal Solution)

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      pH6.86 (25 degrees C)    500mL   –

      019-08393   Amino Acids Mixture Standard Solution, Type H 5Ax1mL –

      019-11941   Active Carbon-impregnated Silica Gel   10g –

      019-19741   Anti Iba1, Rabbit (for Immunocytochemistry)    50μg     –

      025-15481   BES-Thio     1mg      –

      026-16371   BF-170  1mg      –

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      027-01276   Benzyl Alcohol    500mL   100-51-6

      028-03185   Buffer Solution Standard (Phthalate pH Standard Solution)

      pH4.01 (25 degrees C)    500mL   –

      028-03185   Buffer Solution Standard (Phthalate pH Standard Solution)

      pH4.01 (25 degrees C)    500mL   –

      028-03205   Buffer Solution Standard (Tetraborate pH Standard Solution)

      pH9.18 (25 degrees C)    500mL   –

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      077-03155   Gelatin   500g     9000-70-8

      081-00136   Heparin Sodium  10mu    9041-08-1

      088-00705   L-Histidine Hydrochloride Monohydrate      500g     5934-29-2

      093-06471   Insulin, Human, recombinant   50mg    –

      097-06474   Insulin, Human, recombinant   1g   11061-68-0

      120-04891   L-012    100mg   –

      Wako   019-19741,小胶质细胞/巨噬细胞特异性抗体

      125-05061   Lysyl Endopeptidase, Mass Spectrometry Grade      5×20μg  –

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      125-05061   Lysyl Endopeptidase, Mass Spectrometry Grade      5×20μg  –

      125-05061   Lysyl Endopeptidase, Mass Spectrometry Grade      5×20μg  –

      129-02541   Lysyl Endopeptidase( R)   10AU     –

      129-02541   Lysyl Endopeptidase( R)   10AU     –

      129-02541   Lysyl Endopeptidase( R)   10AU     –

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      129-04861   LY 294002   5mg      154447-36-6

      130-07871   Melibiose     5g   585-99-9

      028-16211   BES-So (Cell-impermeant)      1mg      –

      028-17811   BES-H2O2-Ac     1mg      –

      029-16361   BF-168  1mg      –

      039-23511   CultureSure? Freezing Medium    100ml

      Wako   019-19741,小胶质细胞/巨噬细胞特异性抗体–

      041-18861   Dexamethasine   1g   50-02-2

      042-29445   Dibasic Sodium Phosphate Hydrate 500g     10039-32-4

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      043-22611   Dextran 200,000 100g     9004-54-0

      064-05381   Fibroblast Growth Factor (basic)(FGFb / bFGF / FGF2),

      Human, recombinant, Animal-derived-free  50ug     106096-93-9

      071-02293   gamma-Globulin, from Human Serum   5g   9007-83-4

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      075-03075   Gellan Gum  500g     71010-52-1

      131-00405   Magnesium Sulfate    500g     10034-, 99-8

      136-03032   Methyl Red   25g 493-52-7

      137-06085   Molecular Sieves 4A1/16  500g     70955-01-0

      138-07872   Melibiose     25g 585-99-9

      145-08221   Nuclease P1 500u     54576-84-0

      145-08221   Nuclease P1 500u     54576-84-0

      162-09115   Polyethylene Glycol 4,000 500g     25322-68-3

      163-03545   Potassium Chloride    500g     7447-40-7

      164-21655   N/400 Potassium Polyvinyl Sulfate Solu 500mL   26837-42-3

      191-01785   Trisodium Citrate Dihydrate    500g     6132-04-3

      193-02041   Glycerol 2-Phosphate Disodium Salt n-Hydrate   100g     819-83-0

      195-12854   Streptavidin, from Streptomyces avidinii     5mg      9013-20-1

      195-16031   StemSure (R) Freezing Medium     100ml    –

      196-00015   Sucrose 500g     57-50-1

      196-08675   Sodium Dodecyl Sulfate    500g     151-21-3

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      Wako   019-19741,小胶质细胞/巨噬细胞特异性抗体

      197-07485   Sodium Sulfate   500g     7757-82-6

      199-02825   Sodium Dihydrogenphosphate Dihydrate    500g     13472-35-0

      203-17561   Trichostatin A     1mg      58880-19-6

      207-00055   L(+)-Tartaric Acid     500g     87-69-4

      208-08385   1mol/l Triethylammonium Hydrogencarbonate Solution   500mL   15715-58-9

      223-02112   VA-044  25g 27776-21-2

      225-02111   VA-044  100g     27776-21-2

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      165-09105   Polyethylene Glycol 2,000 500g     25322-68-3

      165-17035   Polyvinylpyrrolidone K 30 500g     9003-39-8

      167-09045   Polyethylene Glycol 200   500g     25322-68-3

      169-04245   Potassium Dihydrogen Phosphate  500g     7778-77-0

      169-09125   Polyethylene Glycol 6,000 500g     25322-68-3

      185-01003   Rifampicin    5g   13292-46-1

      186-01114   all-trans-Retinoic Acid 50mg    302-79-4

      189-01001   Rifampicin    1g   13292-46-1

      190-05535   Trisodium Citrate Dihydrate    500g     6132-04-3

      Wako   019-19741,小胶质细胞/巨噬细胞特异性抗体

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      191-01665   Sodium Chloride  500g     7647-14-5

      227-01071   Valproic Acid 5g   99-66-1

      231-02011   Wetting Tension Test Mixture No.52.0  50mL    –

      232-01941   Wetting Tension Test Mixture No.42.0  50mL    –

      233-01971   Wetting Tension Test Mixture No.45.0  50mL    –

      234-02001   Wetting Tension Test Mixture No.50.0  50mL

      Wako   019-19741,小胶质细胞/巨噬细胞特异性抗体–

      235-01931   Wetting Tension Test Mixture No.41.0  50mL    –

      235-02031   Wetting Tension Test Mixture No.56.0  50mL    –

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      236-01961   Wetting Tension Test Mixture No.44.0  50mL    –

      238-01921   Wetting Tension Test Mixture No.40.0  50mL    –

      238-02021   Wetting Tension Test Mixture No.54.0  50mL    –

      238-02141   Wetting Tension Test Mixture No.73.0  50mL    7732-18-5

      253-00513   Y-27632 5mg      331752-47-7

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      257-00511   Y-27632 1mg      331752-47-7

      290-34251   Presep-C DNPH   20EA     –

      296-63801   LabAssay? Phospholipid   1300tests     –

      298-64101   Fructooligosaccharides standard set     set  –

      302-93561   Phos-tag (TM) Agarose    0.5mL    –

      电话：021-60498804  13636613774  QQ：1715451510

      304-93521   Phos-tag (TM) Acrylamide AAL-107      10mg    –

      304-93521   Phos-tag (TM) Acrylamide AAL-107      10mg    –

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      290-34251   Presep-C DNPH   20EA     –

      290-63701   LabAssay? Triglyceride    1000tests     -2016-2-29

      290-65901   LabAssay? Creatinine      500tests –

      291-58601   Lab Assay ALP    900tests –

      293-51601   Endotoxin Extracting Solution for LAL Test  10mL x 4     –

      294-62001   DsDD cDNA Subtraction kit Wako   5tests    –

      294-63601   LabAssay? NEFA 750tests –

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      Wako   019-19741,小胶质细胞/巨噬细胞特异性抗体

      294-65801   LabAssay? Cholesterol    1000tests     –

      295-50201   DNA Extractor? Kit   50tests  –

      295-51301   Limulus ES-II Single Test Wako     25 tests –

      295-51301   Limulus ES-II Single Test Wako     25 tests –

      295-73401   Fructooligosaccharides Set      Set  –

      304-93526   Phos-tag (TM) Acrylamide AAL-107 5mM Aqueous Solution   0.3ml    –

      305-93551   Phos-tag? Mass Analytical Kit set  –

      308-93541   Phos-tag (TM) Biotin BTL-105 10mg    –

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      308-93563   Phos-tag (TM) Agarose    3mL      –

      308-97201   Phos-tag Biotin BTL-1111 1mM Aqueous Solution    0.1000mL    –

      325-88521   Lipase PS Amano SD  10g –

      298-65701   LabAssay? Glucose   1000tests     –

      300-93523   Phos-tag (TM) Acrylamide AAL-107      2mg      –

      301-93531   Phos-tag (TM) Biotin BTL-104 10mg    –

      301-99413   Phos-tagTM Biotin BTL-104 1mM Aqueous Solution[for Campaign]     0.1mL      753451-66-0

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货

      302-93522   Phos-tagTM Acrylamide AAL-107 5mM Aqueous Solution[for Campaign]      0.3mL    871839-54-2

      631-00651   Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0)     20mL    –

      637-00653   Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0)     100mL   –

      637-00653   Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0)     100mL   –

      638-01021   Endotoxin Standad /JPSE  10000un      –

      986-10001   Anti asialo GM1 (Rabbit), (014-09801)  1mL      617-45-8

      Wako 019-11941 Active Carbon-impregnated Silica Gel 现货