Replacement Information

Replacement Information

INCP 000 10 (Protein Applications)

重要规格表

主要应用

西方蛋白印迹

描述

产品目录编号

IPVH00010

Replaces

INCP 000 10（蛋白质应用）

商名

Immobilon

描述

Immobilon-P 卷膜，PVDF，0.45μm，26.5cm x3.75m

背景信息

Overview
– Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.
Immobilon-P Transfer Membrane:
– 45 μm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
– Millipore’s Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity
Immobilon-E Membrane:
– the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
– 45 μm pore size recommended for most western blotting, especially proteins greater than 20 kDa
Blotting Sandwiches:
– When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.
Blotting Filter Paper:
– Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.
Immobilon-PSQ Membrane:
– 0.2 μm pore size and a large internal structure
– Higher protein adsorption and sequencing yields than other membranes
– Recommended for blotting proteins less than 20 kDa
– Prevents blow-through of low molecular weight proteins
Immobilon-FL Membrane:
– The first transfer membrane optimized for fluorescence applications
– Extremely low background improves sensitivity of all fluorescence detection protocols
– Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
– Ideal for multiplexing and chemifluorescence applications
Immobilon PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.
Features & Benefits:
– Won’t crack, curl or fracture when cut
– Low background
– Superior staining capabilities
– Can be reprobed multiple times
Applications:
– Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
– Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
– Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths

产品信息

检测方法

呈色 放射

相容的染色剂

考马斯亮蓝

酰胺黑

印度墨水

丽春红

胶体金

CPTS

甲苯胺蓝

透射显影

Sypro 宝石红

过滤器代码

IPVH

过滤器颜色

白色

Quality Level

MQ400

应用

应用

Western blotting、吸附分析、氨基酸分析、N 端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析

主要应用

西方蛋白印迹

生物信息

主结合机制

静电，疏水

BSA吸收性

215 μg/cm2

山羊IgG吸收性

294 μg/cm2

胰岛素吸收性

160 μg/cm2

可湿润性

疏水

理化信息

孔径

0.45 μm

尺寸

过滤器表面

平纹

过滤器尺寸

26.5 cm x 3.75 m

材料信息

化学

疏水性聚偏氟乙烯（PVDF）

包装信息

数量

1