Nugen RNA-Seq文库构建试剂盒Revelo是Nugen Trio RNA-Seq文库构建试剂盒的简洁版试剂盒

Nugen RNA-Seq文库构建试剂盒

Revelo RNA-Seq文库构建试剂盒是Nugen Trio RNA-Seq文库构建试剂盒的简洁版试剂盒,主要满足技术人员在一天的工作时间内完成样品的文库构建,方便样品在当天上样illumina的测序平台(如Miseq等),第二天可获得高通量测序数据结果。

Revelo RNA-Seq文库构建试剂盒是Nugen Trio RNA-Seq文库构建试剂盒的简洁版试剂盒,主要满足技术人员在一天的工作时间内完成样品的文库构建,方便样品在当天上样illumina的测序平台(如Miseq等),第二天可获得高通量测序数据结果。

该试剂盒延续了Trio试剂盒从降解或混合的微量样本中构建RNA-Seq文库,其中对低丰度序列(如RNA病毒序列)的发现非常重要。大多数市场上的 RNA-Seq 试剂盒不足以灵敏地发现来自高人类背景样本(如人类核糖体RNA)的低拷贝病毒或其他病原。根据样本收集方法的不同,这些样本可能具有大范围的背景序列污染,从而影响有效从样本中发现地拷贝的未知病毒的概率,特别是针对无症状的病毒携带者。

Revelo RNA-Seq 试剂盒是一个完整的RNA测序的解决方案,提供人类总 RNA 样本的从头到尾的NGS文库构建,可以在 6.5 小时内完成总RNA 文库制备(包括文库定量),样本RNA的起始量低至 250 pg。

Revelo RNA-Seq 文库制备试剂盒具有多项*优势:

1.新的 SPIAboost™ 技术可改善人类 rRNA序列的去除,从而最大限度地提高有效reads数

2.无偏向的酶切打断技术,有利于自动化操作

3.双UDI Index,避免Index跳跃的错误测序结果

4.使用减少引物二聚体的DimerFree® 技术

5.NuQuant® 文库定量无需昂贵、耗时的定量方法,例如 qPCR。使用 Qubit进行简单的荧光测量,可以在几分钟内对文库进行定量

6.可应用于NGS 自动文库构建工作站

应用领域:

总RNA 测序 (RNA-Seq)
全转录组分析
基因表达
病原体发现
病毒检测

NuGEN系列产品

NuGEN #0360-08 Celero DNA-Seq , 8rxn
NuGEN #0360-24 Celero DNA-Seq , 24rxn
NuGEN #0357-08 Trio RNA-Seq , 8rxn
NuGEN #0357-32 Trio RNA-Seq , 32rxn
NuGEN #0357-96 Trio RNA-Seq , 96rxn
NuGEN #0508-08 Universal Plus mRNA-Seq , 8rxn
NuGEN #0508-32 Universal Plus mRNA-Seq , 32rxn
NuGEN #0508-96 Universal Plus mRNA-Seq , 96rxn
NuGEN #0508-A01 Universal Plus mRNA-Seq , 96rxn
NuGEN #0520-24 Universal Plus mRNA-Seq with NuQuant , 24rxn
NuGEN #0520-A01 Universal Plus mRNA-Seq with NuQuant , 96rxn
NuGEN #0500-32 Ovation® SoLo RNA-Seq System, Human , 32rxn
NuGEN #0500-96 Ovation® SoLo RNA-Seq System, Human , 96rxn
NuGEN #0501-32 Ovation® SoLo RNA-Seq System, Mouse , 32rxn
NuGEN #0501-96 Ovation® SoLo RNA-Seq System, Mouse , 96rxn
NuGEN #7102-08 Ovation® RNA-Seq System V2 , 8rxn
NuGEN #7102-32 Ovation® RNA-Seq System V2 , 32rxn
NuGEN #7102-A01 Ovation® RNA-Seq System V2 , 96rxn
NuGEN #7150-08 Ovation® RNA-Seq FFPE System , 8rxn
NuGEN #0335-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 1-8 , 32rxn
NuGEN #0336-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 9-16 , 32rxn
NuGEN #9113-A01 Ovation® Ultralow Methyl-Seq System , 96rxn
NuGEN #0535-32 Ovation Ultralow Methy-Seq DR Multiplex System 1-8, with TrueMethyl® oxBS , 32rxn
NuGEN #0536-32 Ovation Ultralow Methy-Seq DR Multiplex System 9-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0553-32 Ovation RRBS Methyl-Seq System 1-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0414-32 TrueMethyl® oxBS Module , 32rxn
NuGEN #3302-12 Ovation® Pico WTA System V2 , 12rxn
NuGEN #3302-60 Ovation® Pico WTA System V2 , 60rxn
NuGEN #3302-A01 Ovation® Pico WTA System V2 , 96rxn
NuGEN #3403-12 Ovation® FFPE WTA System , 12rxn
NuGEN #3403-60 Ovation® FFPE WTA System , 60rxn
NuGEN #3403-A01 Ovation® FFPE WTA System , 96rxn
NuGEN #3100-12 Ovation® RNA Amplification System V2 , 12rxn
NuGEN #3100-60 Ovation® RNA Amplification System V2 , 60rxn
NuGEN #3100-A01 Ovation® RNA Amplification System V2 , 96rxn
NuGEN #0344-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-08 Ovation® Ultralow System V2 , 8rxn
NuGEN #0344NB-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-A01 Ovation® Ultralow System V2 , 96rxn

NuGEN代理,RNA病毒文库制备试剂盒,Universal RNA-Seq文库制备试剂盒是一种整体解决方案

Universal RNA-Seq文库制备试剂盒是一种整体解决方案,用于链特异性RNA-Seq文库的构建,使用的是纳克量的从多种组织或细胞系中获得的总RNA。 该工作流程与从FFPE样品中提取的高质量RNA和降解RNA兼容。
该试剂盒包括AnyDeplete技术,用于消除不需要的转录序列。 AnyDeplete可以应用于多种转录本,以减少无用信息的测序数据,从而更有效地利用测序通量。 该试剂盒还具有NuQuant,NuQuant是一种基于荧光的新颖方法,可在几分钟内定量分析文库摩尔浓度。 NuQuant减少了与标准库质量控制相关的时间和错误。
带NuQuant的Universal RNA-Seq试剂盒提供32或96个的标签接头,可进行多重测序,以进一步优化转录组分析过程中的效率并节省成本。

将文库制备和文库定量整合的全转录组RNA-Seq试剂盒。
该试剂盒结合了一系列专有创新技术,可为链特异性RNA-Seq文库提供高效经济的整体解决方案。

NuGEN系列产品

NuGEN #0360-08 Celero DNA-Seq , 8rxn
NuGEN #0360-24 Celero DNA-Seq , 24rxn
NuGEN #0357-08 Trio RNA-Seq , 8rxn
NuGEN #0357-32 Trio RNA-Seq , 32rxn
NuGEN #0357-96 Trio RNA-Seq , 96rxn
NuGEN #0508-08 Universal Plus mRNA-Seq , 8rxn
NuGEN #0508-32 Universal Plus mRNA-Seq , 32rxn
NuGEN #0508-96 Universal Plus mRNA-Seq , 96rxn
NuGEN #0508-A01 Universal Plus mRNA-Seq , 96rxn
NuGEN #0520-24 Universal Plus mRNA-Seq with NuQuant , 24rxn
NuGEN #0520-A01 Universal Plus mRNA-Seq with NuQuant , 96rxn
NuGEN #0500-32 Ovation® SoLo RNA-Seq System, Human , 32rxn
NuGEN #0500-96 Ovation® SoLo RNA-Seq System, Human , 96rxn
NuGEN #0501-32 Ovation® SoLo RNA-Seq System, Mouse , 32rxn
NuGEN #0501-96 Ovation® SoLo RNA-Seq System, Mouse , 96rxn
NuGEN #7102-08 Ovation® RNA-Seq System V2 , 8rxn
NuGEN #7102-32 Ovation® RNA-Seq System V2 , 32rxn
NuGEN #7102-A01 Ovation® RNA-Seq System V2 , 96rxn
NuGEN #7150-08 Ovation® RNA-Seq FFPE System , 8rxn
NuGEN #0335-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 1-8 , 32rxn
NuGEN #0336-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 9-16 , 32rxn
NuGEN #9113-A01 Ovation® Ultralow Methyl-Seq System , 96rxn
NuGEN #0535-32 Ovation Ultralow Methy-Seq DR Multiplex System 1-8, with TrueMethyl® oxBS , 32rxn
NuGEN #0536-32 Ovation Ultralow Methy-Seq DR Multiplex System 9-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0553-32 Ovation RRBS Methyl-Seq System 1-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0414-32 TrueMethyl® oxBS Module , 32rxn
NuGEN #3302-12 Ovation® Pico WTA System V2 , 12rxn
NuGEN #3302-60 Ovation® Pico WTA System V2 , 60rxn
NuGEN #3302-A01 Ovation® Pico WTA System V2 , 96rxn
NuGEN #3403-12 Ovation® FFPE WTA System , 12rxn
NuGEN #3403-60 Ovation® FFPE WTA System , 60rxn
NuGEN #3403-A01 Ovation® FFPE WTA System , 96rxn
NuGEN #3100-12 Ovation® RNA Amplification System V2 , 12rxn
NuGEN #3100-60 Ovation® RNA Amplification System V2 , 60rxn
NuGEN #3100-A01 Ovation® RNA Amplification System V2 , 96rxn
NuGEN #0344-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-08 Ovation® Ultralow System V2 , 8rxn
NuGEN #0344NB-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-A01 Ovation® Ultralow System V2 , 96rxn

Nugen代理 0363-32,Ovation通用细菌转录组文库制备试剂盒

Ovation通用细菌转录组文库制备试剂盒 适用于微生物组学及微生物表达的测序应用。

Ovation®全原核RNA-Seq文库制备试剂盒是一种用于微生物RNA测序研究的的理想选择。 该RNA-Seq试剂盒使用从纯培养物或混合细菌群获得的100ng至500ng总RNA,为方向链RNA-Seq文库构建提供了完整的解决方案。 整合了AnyDeplete技术可以从广泛的原核生物中消除rRNA,从而优化有用reads并降低测序成本。

样品类型包括来自细胞系的纯化RNA,环境样品和微生物组研究。

主要特点

1.完整的原核转录组学解决方案,集成rRNA耗尽的AnyDeplete技术

2.广泛的样品量范围从100 ng到500 ng

3.与具有广泛GC含量的样品兼容

NuGEN系列产品

NuGEN #0360-08 Celero DNA-Seq , 8rxn
NuGEN #0360-24 Celero DNA-Seq , 24rxn
NuGEN #0357-08 Trio RNA-Seq , 8rxn
NuGEN #0357-32 Trio RNA-Seq , 32rxn
NuGEN #0357-96 Trio RNA-Seq , 96rxn
NuGEN #0508-08 Universal Plus mRNA-Seq , 8rxn
NuGEN #0508-32 Universal Plus mRNA-Seq , 32rxn
NuGEN #0508-96 Universal Plus mRNA-Seq , 96rxn
NuGEN #0508-A01 Universal Plus mRNA-Seq , 96rxn
NuGEN #0520-24 Universal Plus mRNA-Seq with NuQuant , 24rxn
NuGEN #0520-A01 Universal Plus mRNA-Seq with NuQuant , 96rxn
NuGEN #0500-32 Ovation® SoLo RNA-Seq System, Human , 32rxn
NuGEN #0500-96 Ovation® SoLo RNA-Seq System, Human , 96rxn
NuGEN #0501-32 Ovation® SoLo RNA-Seq System, Mouse , 32rxn
NuGEN #0501-96 Ovation® SoLo RNA-Seq System, Mouse , 96rxn
NuGEN #7102-08 Ovation® RNA-Seq System V2 , 8rxn
NuGEN #7102-32 Ovation® RNA-Seq System V2 , 32rxn
NuGEN #7102-A01 Ovation® RNA-Seq System V2 , 96rxn
NuGEN #7150-08 Ovation® RNA-Seq FFPE System , 8rxn
NuGEN #0335-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 1-8 , 32rxn
NuGEN #0336-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 9-16 , 32rxn
NuGEN #9113-A01 Ovation® Ultralow Methyl-Seq System , 96rxn
NuGEN #0535-32 Ovation Ultralow Methy-Seq DR Multiplex System 1-8, with TrueMethyl® oxBS , 32rxn
NuGEN #0536-32 Ovation Ultralow Methy-Seq DR Multiplex System 9-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0553-32 Ovation RRBS Methyl-Seq System 1-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0414-32 TrueMethyl® oxBS Module , 32rxn
NuGEN #3302-12 Ovation® Pico WTA System V2 , 12rxn
NuGEN #3302-60 Ovation® Pico WTA System V2 , 60rxn
NuGEN #3302-A01 Ovation® Pico WTA System V2 , 96rxn
NuGEN #3403-12 Ovation® FFPE WTA System , 12rxn
NuGEN #3403-60 Ovation® FFPE WTA System , 60rxn
NuGEN #3403-A01 Ovation® FFPE WTA System , 96rxn
NuGEN #3100-12 Ovation® RNA Amplification System V2 , 12rxn
NuGEN #3100-60 Ovation® RNA Amplification System V2 , 60rxn
NuGEN #3100-A01 Ovation® RNA Amplification System V2 , 96rxn
NuGEN #0344-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-08 Ovation® Ultralow System V2 , 8rxn
NuGEN #0344NB-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-A01 Ovation® Ultralow System V2 , 96rxn

Celero 宏基因组文库制备试剂盒,Nugen代理 0360

Celero具有快速,易于使用,仅加样的工作流程,无需连接接头后的磁珠纯化步骤。试剂盒还包括了NuGEN的突破性文库定量方法NuQuant®,它可以节省测量文库浓度的时间和成本。使用经过验证的DimerFree™技术,您可以在单个试剂盒使用10 ng至1μg的完整DNA和超过200 ng的FFPE DNA,而无需担心GC含量引入的偏差。

Celero DNA-Seq文库制备试剂盒具有以下几个*的优点:

1. 简短且简单的工作流程:通过3个加样步骤准备NGS文库,减少手动操作时间,无需接头或模板稀释或连接后磁珠纯化。

2. 无接头二聚体:与需要多步纯化的竞争对手相比,可获得更多有效序列信息。

3. 灵活的多重应用:试剂盒中包含的每个反应的单index或*双index接头。

4. 灵活的工作流程:使用相同试剂盒的PCR和无PCR工作流程,提供灵活性并减少需要购买和存储的试剂盒数量。

5. 集成的文库定量:快速确定文库的摩尔浓度,无需昂贵且耗时的过程。

6. 自动化:兼容多个移液工作站平台。

Celero 宏基因组文库制备试剂盒,Nugen代理 0360

Celero DNA-Seq文库制备试剂盒是一种创新试剂盒,旨在帮助研究人员简化Illumina测序仪的文库制备。

NuGEN系列产品

NuGEN #0360-08 Celero DNA-Seq , 8rxn
NuGEN #0360-24 Celero DNA-Seq , 24rxn
NuGEN #0357-08 Trio RNA-Seq , 8rxn
NuGEN #0357-32 Trio RNA-Seq , 32rxn
NuGEN #0357-96 Trio RNA-Seq , 96rxn
NuGEN #0508-08 Universal Plus mRNA-Seq , 8rxn
NuGEN #0508-32 Universal Plus mRNA-Seq , 32rxn
NuGEN #0508-96 Universal Plus mRNA-Seq , 96rxn
NuGEN #0508-A01 Universal Plus mRNA-Seq , 96rxn
NuGEN #0520-24 Universal Plus mRNA-Seq with NuQuant , 24rxn
NuGEN #0520-A01 Universal Plus mRNA-Seq with NuQuant , 96rxn
NuGEN #0500-32 Ovation® SoLo RNA-Seq System, Human , 32rxn
NuGEN #0500-96 Ovation® SoLo RNA-Seq System, Human , 96rxn
NuGEN #0501-32 Ovation® SoLo RNA-Seq System, Mouse , 32rxn
NuGEN #0501-96 Ovation® SoLo RNA-Seq System, Mouse , 96rxn
NuGEN #7102-08 Ovation® RNA-Seq System V2 , 8rxn
NuGEN #7102-32 Ovation® RNA-Seq System V2 , 32rxn
NuGEN #7102-A01 Ovation® RNA-Seq System V2 , 96rxn
NuGEN #7150-08 Ovation® RNA-Seq FFPE System , 8rxn
NuGEN #0335-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 1-8 , 32rxn
NuGEN #0336-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 9-16 , 32rxn
NuGEN #9113-A01 Ovation® Ultralow Methyl-Seq System , 96rxn
NuGEN #0535-32 Ovation Ultralow Methy-Seq DR Multiplex System 1-8, with TrueMethyl® oxBS , 32rxn
NuGEN #0536-32 Ovation Ultralow Methy-Seq DR Multiplex System 9-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0553-32 Ovation RRBS Methyl-Seq System 1-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0414-32 TrueMethyl® oxBS Module , 32rxn
NuGEN #3302-12 Ovation® Pico WTA System V2 , 12rxn
NuGEN #3302-60 Ovation® Pico WTA System V2 , 60rxn
NuGEN #3302-A01 Ovation® Pico WTA System V2 , 96rxn
NuGEN #3403-12 Ovation® FFPE WTA System , 12rxn
NuGEN #3403-60 Ovation® FFPE WTA System , 60rxn
NuGEN #3403-A01 Ovation® FFPE WTA System , 96rxn
NuGEN #3100-12 Ovation® RNA Amplification System V2 , 12rxn
NuGEN #3100-60 Ovation® RNA Amplification System V2 , 60rxn
NuGEN #3100-A01 Ovation® RNA Amplification System V2 , 96rxn
NuGEN #0344-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-08 Ovation® Ultralow System V2 , 8rxn
NuGEN #0344NB-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-A01 Ovation® Ultralow System V2 , 96rxn

NuGEN 0506 Trio微量样本全转录组文库制备试剂盒

Trio RNA-Seq文库制备试剂盒提供同类更佳的NGS文库制备系统和RNA测序解决方案,用于液体活检,FFPE和其他微量的挑战性样品。

Trio微量RNA-Seq专为稳定可靠的RNA-Seq文库制备而设计,可与500 pg-50 ng的总RNA配合使用,可帮助研究人员从几乎任何样本中捕获完整的生物学信息。

更新的Trio微量RNA-Seq文库制备试剂盒现在集成了来自大量文献发表的Ovation®RNA-Seq System V2和双重index的成熟技术,可提供从低样本量或低频转录本的总RNA到完整文库制备的准确流程。

NuGEN的双重index可以方便地以预装微孔板96个的条形码对,因此无需手动准备条形码对。只需将接头添加到样品中并连接,每个样品将具有index1和index2条形码,这些条形码与平板中的所有其他样品不同。

Trio微量RNA-Seq文库制备结合了三种强大的技术:

1.单引物等温扩增技术(SPIA)可以扩增微量的和降解的样品。

2. 酶切片段化和DimerFree文库构建激素允许高效和稳定的文库制备。

3.在构建文库后,AnyDeplete技术可定制转录本耗尽方案,大化信息序列reads全转录组数据。

这种的工作流程为整个转录组文库提供了无接头二聚体和整合的无用转录本耗尽,从而增强了检测和更均匀的转录本覆盖率。

NuGEN系列产品

NuGEN #0360-08 Celero DNA-Seq , 8rxn
NuGEN #0360-24 Celero DNA-Seq , 24rxn
NuGEN #0357-08 Trio RNA-Seq , 8rxn
NuGEN #0357-32 Trio RNA-Seq , 32rxn
NuGEN #0357-96 Trio RNA-Seq , 96rxn
NuGEN #0508-08 Universal Plus mRNA-Seq , 8rxn
NuGEN #0508-32 Universal Plus mRNA-Seq , 32rxn
NuGEN #0508-96 Universal Plus mRNA-Seq , 96rxn
NuGEN #0508-A01 Universal Plus mRNA-Seq , 96rxn
NuGEN #0520-24 Universal Plus mRNA-Seq with NuQuant , 24rxn
NuGEN #0520-A01 Universal Plus mRNA-Seq with NuQuant , 96rxn
NuGEN #0500-32 Ovation® SoLo RNA-Seq System, Human , 32rxn
NuGEN #0500-96 Ovation® SoLo RNA-Seq System, Human , 96rxn
NuGEN #0501-32 Ovation® SoLo RNA-Seq System, Mouse , 32rxn
NuGEN #0501-96 Ovation® SoLo RNA-Seq System, Mouse , 96rxn
NuGEN #7102-08 Ovation® RNA-Seq System V2 , 8rxn
NuGEN #7102-32 Ovation® RNA-Seq System V2 , 32rxn
NuGEN #7102-A01 Ovation® RNA-Seq System V2 , 96rxn
NuGEN #7150-08 Ovation® RNA-Seq FFPE System , 8rxn
NuGEN #0335-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 1-8 , 32rxn
NuGEN #0336-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 9-16 , 32rxn
NuGEN #9113-A01 Ovation® Ultralow Methyl-Seq System , 96rxn
NuGEN #0535-32 Ovation Ultralow Methy-Seq DR Multiplex System 1-8, with TrueMethyl® oxBS , 32rxn
NuGEN #0536-32 Ovation Ultralow Methy-Seq DR Multiplex System 9-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0553-32 Ovation RRBS Methyl-Seq System 1-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0414-32 TrueMethyl® oxBS Module , 32rxn
NuGEN #3302-12 Ovation® Pico WTA System V2 , 12rxn
NuGEN #3302-60 Ovation® Pico WTA System V2 , 60rxn
NuGEN #3302-A01 Ovation® Pico WTA System V2 , 96rxn
NuGEN #3403-12 Ovation® FFPE WTA System , 12rxn
NuGEN #3403-60 Ovation® FFPE WTA System , 60rxn
NuGEN #3403-A01 Ovation® FFPE WTA System , 96rxn
NuGEN #3100-12 Ovation® RNA Amplification System V2 , 12rxn
NuGEN #3100-60 Ovation® RNA Amplification System V2 , 60rxn
NuGEN #3100-A01 Ovation® RNA Amplification System V2 , 96rxn
NuGEN #0344-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-08 Ovation® Ultralow System V2 , 8rxn
NuGEN #0344NB-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-A01 Ovation® Ultralow System V2 , 96rxn

NuGEN代理 超微量样本宏基因组文库制备试剂盒 0344

超微量样本宏基因组文库制备试剂盒 0344

样品类型包括从细胞系,新鲜和FFPE组织,液体活组织检查和细胞游离DNA中产生的纯化DNA或cDNA。该试剂盒兼容混合捕获方法,包括Agilent SureSelect和Nimblegen SeqCap。

Ovation Ultralow V2 DNA-Seq文库制备试剂盒具有以下几个优势:

1.简短,简单,工作流程:NGS文库制备只需3小时,无隐藏标准化或适配器稀释步骤。

2.任何样品类型的超微量范围:适用于任何样品类型的一个试剂盒,包括样品量少,完整或片段化的DNA。

3.没有适配器二聚体:与需要多步纯化的竞争对手相比,获得更多有效序列信息。

4. 灵活的多重应用:试剂盒中包含的每个反应的单index或双index接头。

5. 广泛的覆盖范围:可对广泛的GC含量范围进行高保真扩增。

6. 自动化:兼容多个移液工作站平台。

NuGEN系列产品

NuGEN #0360-08 Celero DNA-Seq , 8rxn
NuGEN #0360-24 Celero DNA-Seq , 24rxn
NuGEN #0357-08 Trio RNA-Seq , 8rxn
NuGEN #0357-32 Trio RNA-Seq , 32rxn
NuGEN #0357-96 Trio RNA-Seq , 96rxn
NuGEN #0508-08 Universal Plus mRNA-Seq , 8rxn
NuGEN #0508-32 Universal Plus mRNA-Seq , 32rxn
NuGEN #0508-96 Universal Plus mRNA-Seq , 96rxn
NuGEN #0508-A01 Universal Plus mRNA-Seq , 96rxn
NuGEN #0520-24 Universal Plus mRNA-Seq with NuQuant , 24rxn
NuGEN #0520-A01 Universal Plus mRNA-Seq with NuQuant , 96rxn
NuGEN #0500-32 Ovation® SoLo RNA-Seq System, Human , 32rxn
NuGEN #0500-96 Ovation® SoLo RNA-Seq System, Human , 96rxn
NuGEN #0501-32 Ovation® SoLo RNA-Seq System, Mouse , 32rxn
NuGEN #0501-96 Ovation® SoLo RNA-Seq System, Mouse , 96rxn
NuGEN #7102-08 Ovation® RNA-Seq System V2 , 8rxn
NuGEN #7102-32 Ovation® RNA-Seq System V2 , 32rxn
NuGEN #7102-A01 Ovation® RNA-Seq System V2 , 96rxn
NuGEN #7150-08 Ovation® RNA-Seq FFPE System , 8rxn
NuGEN #0335-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 1-8 , 32rxn
NuGEN #0336-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 9-16 , 32rxn
NuGEN #9113-A01 Ovation® Ultralow Methyl-Seq System , 96rxn
NuGEN #0535-32 Ovation Ultralow Methy-Seq DR Multiplex System 1-8, with TrueMethyl® oxBS , 32rxn
NuGEN #0536-32 Ovation Ultralow Methy-Seq DR Multiplex System 9-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0553-32 Ovation RRBS Methyl-Seq System 1-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0414-32 TrueMethyl® oxBS Module , 32rxn
NuGEN #3302-12 Ovation® Pico WTA System V2 , 12rxn
NuGEN #3302-60 Ovation® Pico WTA System V2 , 60rxn
NuGEN #3302-A01 Ovation® Pico WTA System V2 , 96rxn
NuGEN #3403-12 Ovation® FFPE WTA System , 12rxn
NuGEN #3403-60 Ovation® FFPE WTA System , 60rxn
NuGEN #3403-A01 Ovation® FFPE WTA System , 96rxn
NuGEN #3100-12 Ovation® RNA Amplification System V2 , 12rxn
NuGEN #3100-60 Ovation® RNA Amplification System V2 , 60rxn
NuGEN #3100-A01 Ovation® RNA Amplification System V2 , 96rxn
NuGEN #0344-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-08 Ovation® Ultralow System V2 , 8rxn
NuGEN #0344NB-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-A01 Ovation® Ultralow System V2 , 96rxn

NuGEN代理 病毒RNA富集扩增试剂盒 7102 推出了Ovation RNA-Seq System V2

NuGEN不断创新,推出了Ovation RNA-Seq System V2。该体系利用NuGEN的Ribo-SPIA技术,只需4.5小时,即可从500 pg-100 ng的总RNA中获得高质量的cDNA样品。

Ovation RNA-Seq System V2以简化的工作流程提供了更高的转录本覆盖和均一的测序读长分布;从样品到转录组测序。该系统使科研人员能够利用少量的总RNA开展RNA-Seq研究,在poly(A)+ RNA和非多腺苷酸化转录本全长上具有均一的读长覆盖。

扩增起始于每个样品的3’末端,并可在整个转录组中随机开始。因此,NuGEN的Ovation RNA-Seq System V2能够为您提供更完整的转录组图片,不需要rRNA去除或poly(A)筛选的额外成本。

Ovation RNA-Seq System V2提供了优化的预混试剂以及能够批量处理8-32个总RNA样品的流程。同时它也可作为批量处理96个样品的自动化解决方案。

NuGEN代理 病毒RNA富集扩增试剂盒 7102

• 低RNA起始量要求:
只需500 pg–100ng总RNA,即可通过mRNA和非多聚腺苷酸转录本获得RNA-Seq数据
• 更全面的转录组表征:
提供增强的转录组测序覆盖深度,无5’/3’位置偏向性
• 基于成熟技术的快速、简单流程:
使用NuGEN强大的Ribo-SPIA技术产生双链cDNA,可在4.5小时内完成
RNA-Seq文库构建,无需进行poly(A)筛选及rRNA去除

NuGEN系列产品

NuGEN #0360-08 Celero DNA-Seq , 8rxn
NuGEN #0360-24 Celero DNA-Seq , 24rxn
NuGEN #0357-08 Trio RNA-Seq , 8rxn
NuGEN #0357-32 Trio RNA-Seq , 32rxn
NuGEN #0357-96 Trio RNA-Seq , 96rxn
NuGEN #0508-08 Universal Plus mRNA-Seq , 8rxn
NuGEN #0508-32 Universal Plus mRNA-Seq , 32rxn
NuGEN #0508-96 Universal Plus mRNA-Seq , 96rxn
NuGEN #0508-A01 Universal Plus mRNA-Seq , 96rxn
NuGEN #0520-24 Universal Plus mRNA-Seq with NuQuant , 24rxn
NuGEN #0520-A01 Universal Plus mRNA-Seq with NuQuant , 96rxn
NuGEN #0500-32 Ovation® SoLo RNA-Seq System, Human , 32rxn
NuGEN #0500-96 Ovation® SoLo RNA-Seq System, Human , 96rxn
NuGEN #0501-32 Ovation® SoLo RNA-Seq System, Mouse , 32rxn
NuGEN #0501-96 Ovation® SoLo RNA-Seq System, Mouse , 96rxn
NuGEN #7102-08 Ovation® RNA-Seq System V2 , 8rxn
NuGEN #7102-32 Ovation® RNA-Seq System V2 , 32rxn
NuGEN #7102-A01 Ovation® RNA-Seq System V2 , 96rxn
NuGEN #7150-08 Ovation® RNA-Seq FFPE System , 8rxn
NuGEN #0335-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 1-8 , 32rxn
NuGEN #0336-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 9-16 , 32rxn
NuGEN #9113-A01 Ovation® Ultralow Methyl-Seq System , 96rxn
NuGEN #0535-32 Ovation Ultralow Methy-Seq DR Multiplex System 1-8, with TrueMethyl® oxBS , 32rxn
NuGEN #0536-32 Ovation Ultralow Methy-Seq DR Multiplex System 9-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0553-32 Ovation RRBS Methyl-Seq System 1-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0414-32 TrueMethyl® oxBS Module , 32rxn
NuGEN #3302-12 Ovation® Pico WTA System V2 , 12rxn
NuGEN #3302-60 Ovation® Pico WTA System V2 , 60rxn
NuGEN #3302-A01 Ovation® Pico WTA System V2 , 96rxn
NuGEN #3403-12 Ovation® FFPE WTA System , 12rxn
NuGEN #3403-60 Ovation® FFPE WTA System , 60rxn
NuGEN #3403-A01 Ovation® FFPE WTA System , 96rxn
NuGEN #3100-12 Ovation® RNA Amplification System V2 , 12rxn
NuGEN #3100-60 Ovation® RNA Amplification System V2 , 60rxn
NuGEN #3100-A01 Ovation® RNA Amplification System V2 , 96rxn
NuGEN #0344-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-08 Ovation® Ultralow System V2 , 8rxn
NuGEN #0344NB-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-A01 Ovation® Ultralow System V2 , 96rxn

Nugen代理 甲基化文库制备试剂盒适用于甲基化测序RRBS Methyl-Seq

Nugen甲基化文库制备试剂盒

Ovation RRBS Methyl-Seq甲基化文库制备试剂盒以单碱基分辨率提供全基因组定量DNA甲基化信息。一种简单,快速,可扩展的产生Methyl-Seq文库的解决方案,无需使用PhiX,使用Reduced Representation Bisulfite Sequencing(RRBS)方法分析DNA甲基化。适用的样品是来自广泛的细胞和组织类型的人基因组DNA。

Nugen代理 甲基化文库制备试剂盒适用于甲基化测序RRBS Methyl-Seq

NuGEN Technologies公司现在是TrueMethyl oxBS转换技术的发源地。作为Cambridge Epigenetix的合作伙伴,NuGEN的甲基化测序试剂盒是含有突破性TrueMethyl氧化亚硫酸氢盐转化技术的试剂盒。该方法能够对NGS进行准确的5-甲基胞嘧啶(5mC)鉴定,以及5-羟甲基胞嘧啶(5hmC)的检测,这是一种未通过传统亚硫酸氢盐转化方法测定的修饰碱。

当与Ovation RRBS Methyl-Seq试剂盒结合使用时,结果是准确且低成本的甲基化测序。

Ovation RRBS Methyl-Seq试剂盒的特点:

1.DimerFree™文库制备

2.可整合氧化亚硫酸氢盐转化(TrueMethyl)

3.均一的全基因组覆盖率

4.可重复的RRBS富集

5.使用多种接头进行无PhiX测序

6.使用相同的工作流程检测5mC和5hmC

7.定向文库

8.一体化:从单个试剂盒中实现DNA到可测序文库

Nugen甲基化文库制备试剂盒适用于甲基化测序。

Nugen SoLo 单细胞测序文库制备试剂盒应用

单细胞RNA测序
基因表达
全转录组分析
超微量样品类型
液体活检样本
转录本发现
组织异质性

NuGEN系列产品

NuGEN #0360-08 Celero DNA-Seq , 8rxn
NuGEN #0360-24 Celero DNA-Seq , 24rxn
NuGEN #0357-08 Trio RNA-Seq , 8rxn
NuGEN #0357-32 Trio RNA-Seq , 32rxn
NuGEN #0357-96 Trio RNA-Seq , 96rxn
NuGEN #0508-08 Universal Plus mRNA-Seq , 8rxn
NuGEN #0508-32 Universal Plus mRNA-Seq , 32rxn
NuGEN #0508-96 Universal Plus mRNA-Seq , 96rxn
NuGEN #0508-A01 Universal Plus mRNA-Seq , 96rxn
NuGEN #0520-24 Universal Plus mRNA-Seq with NuQuant , 24rxn
NuGEN #0520-A01 Universal Plus mRNA-Seq with NuQuant , 96rxn
NuGEN #0500-32 Ovation® SoLo RNA-Seq System, Human , 32rxn
NuGEN #0500-96 Ovation® SoLo RNA-Seq System, Human , 96rxn
NuGEN #0501-32 Ovation® SoLo RNA-Seq System, Mouse , 32rxn
NuGEN #0501-96 Ovation® SoLo RNA-Seq System, Mouse , 96rxn
NuGEN #7102-08 Ovation® RNA-Seq System V2 , 8rxn
NuGEN #7102-32 Ovation® RNA-Seq System V2 , 32rxn
NuGEN #7102-A01 Ovation® RNA-Seq System V2 , 96rxn
NuGEN #7150-08 Ovation® RNA-Seq FFPE System , 8rxn
NuGEN #0335-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 1-8 , 32rxn
NuGEN #0336-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 9-16 , 32rxn
NuGEN #9113-A01 Ovation® Ultralow Methyl-Seq System , 96rxn
NuGEN #0535-32 Ovation Ultralow Methy-Seq DR Multiplex System 1-8, with TrueMethyl® oxBS , 32rxn
NuGEN #0536-32 Ovation Ultralow Methy-Seq DR Multiplex System 9-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0553-32 Ovation RRBS Methyl-Seq System 1-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0414-32 TrueMethyl® oxBS Module , 32rxn
NuGEN #3302-12 Ovation® Pico WTA System V2 , 12rxn
NuGEN #3302-60 Ovation® Pico WTA System V2 , 60rxn
NuGEN #3302-A01 Ovation® Pico WTA System V2 , 96rxn
NuGEN #3403-12 Ovation® FFPE WTA System , 12rxn
NuGEN #3403-60 Ovation® FFPE WTA System , 60rxn
NuGEN #3403-A01 Ovation® FFPE WTA System , 96rxn
NuGEN #3100-12 Ovation® RNA Amplification System V2 , 12rxn
NuGEN #3100-60 Ovation® RNA Amplification System V2 , 60rxn
NuGEN #3100-A01 Ovation® RNA Amplification System V2 , 96rxn
NuGEN #0344-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-08 Ovation® Ultralow System V2 , 8rxn
NuGEN #0344NB-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-A01 Ovation® Ultralow System V2 , 96rxn

Nugen代理 SoLo 单细胞测序文库制备试剂盒0500-32

Ovation®SoLo RNA-Seq文库制备试剂盒是一种用于超微量或单细胞RNA测序的文库制备解决方案,可从10 pg至10 ng总RNA或 1-500个细胞的裂解液中生成的方向链的且rRNA去除的文库。 采用灵活定制的AnyDeplete技术,这种超微量RNA-Seq文库制备试剂盒可去除rRNA和其他不需要的高丰度转录本,从而增加测序动态范围,降低了测序成本并简化数据分析。

样品类型包括来自细胞的纯化RNA或裂解物,包括单细胞,新鲜或FFPE组织,液体活组织检查,游离RNA和其他超微量RNA样品。

AnyDeplete标准探针产品有人类、果蝇和小鼠的。 也可以定制需要去除的序列探针。

Nugen代理 SoLo 单细胞测序文库制备试剂盒0500-32

Nugen SoLo 单细胞测序文库制备试剂盒应用

单细胞RNA测序
基因表达
全转录组分析
超微量样品类型
液体活检样本
转录本发现
组织异质性

NuGEN系列产品

NuGEN #0360-08 Celero DNA-Seq , 8rxn
NuGEN #0360-24 Celero DNA-Seq , 24rxn
NuGEN #0357-08 Trio RNA-Seq , 8rxn
NuGEN #0357-32 Trio RNA-Seq , 32rxn
NuGEN #0357-96 Trio RNA-Seq , 96rxn
NuGEN #0508-08 Universal Plus mRNA-Seq , 8rxn
NuGEN #0508-32 Universal Plus mRNA-Seq , 32rxn
NuGEN #0508-96 Universal Plus mRNA-Seq , 96rxn
NuGEN #0508-A01 Universal Plus mRNA-Seq , 96rxn
NuGEN #0520-24 Universal Plus mRNA-Seq with NuQuant , 24rxn
NuGEN #0520-A01 Universal Plus mRNA-Seq with NuQuant , 96rxn
NuGEN #0500-32 Ovation® SoLo RNA-Seq System, Human , 32rxn
NuGEN #0500-96 Ovation® SoLo RNA-Seq System, Human , 96rxn
NuGEN #0501-32 Ovation® SoLo RNA-Seq System, Mouse , 32rxn
NuGEN #0501-96 Ovation® SoLo RNA-Seq System, Mouse , 96rxn
NuGEN #7102-08 Ovation® RNA-Seq System V2 , 8rxn
NuGEN #7102-32 Ovation® RNA-Seq System V2 , 32rxn
NuGEN #7102-A01 Ovation® RNA-Seq System V2 , 96rxn
NuGEN #7150-08 Ovation® RNA-Seq FFPE System , 8rxn
NuGEN #0335-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 1-8 , 32rxn
NuGEN #0336-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 9-16 , 32rxn
NuGEN #9113-A01 Ovation® Ultralow Methyl-Seq System , 96rxn
NuGEN #0535-32 Ovation Ultralow Methy-Seq DR Multiplex System 1-8, with TrueMethyl® oxBS , 32rxn
NuGEN #0536-32 Ovation Ultralow Methy-Seq DR Multiplex System 9-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0553-32 Ovation RRBS Methyl-Seq System 1-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0414-32 TrueMethyl® oxBS Module , 32rxn
NuGEN #3302-12 Ovation® Pico WTA System V2 , 12rxn
NuGEN #3302-60 Ovation® Pico WTA System V2 , 60rxn
NuGEN #3302-A01 Ovation® Pico WTA System V2 , 96rxn
NuGEN #3403-12 Ovation® FFPE WTA System , 12rxn
NuGEN #3403-60 Ovation® FFPE WTA System , 60rxn
NuGEN #3403-A01 Ovation® FFPE WTA System , 96rxn
NuGEN #3100-12 Ovation® RNA Amplification System V2 , 12rxn
NuGEN #3100-60 Ovation® RNA Amplification System V2 , 60rxn
NuGEN #3100-A01 Ovation® RNA Amplification System V2 , 96rxn
NuGEN #0344-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-08 Ovation® Ultralow System V2 , 8rxn
NuGEN #0344NB-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-A01 Ovation® Ultralow System V2 , 96rxn