Sigma丝裂霉素 C 来源于头状链霉菌

产品编号 M0503-2mg | CAS号 50-07-7 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Mitomycin C is produced by a strain of actinomyces, Streptomyces caespitosus. It contains three anticancer moieties, quinine, urethane, and aziridine groups. It is used to generate mitotically inactive feeder cells in cell culture systems, such as the mitotically inactive fibroblasts used in embryonic stem cell systems.

Biochem/physiol Actions

DNA 合成、细胞核分裂和癌细胞的抑制剂。对革兰氏阳性菌、革兰氏阴性菌、抗酸杆菌有抗菌性。

Caution

This vial contains 2 mg Mitomycin C and 48 mg NaCl. Stock solutions should be filter sterilized and stored at 2-8°C in the dark. Solutions at pH 6-9 can be stored at 0-5°C for up to a week, but if a precipitate forms, a fresh solution should be prepared – the precipitated solution has been proven toxic to cells.

Preparation Note

Mitomycin C is soluble in water at .5 mg/mL, with a pH of 6.0-7.5. It undergoes rapid degradation in acidic solutions with pH<6, and is mostly likely to retain activity in solutions with a pH between 6-9.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Sigma 阿霉素 盐酸盐

产品编号 D1515 | CAS号 25316-40-9 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

包装:10 mg in glass bottle Biochem/physiol Actions

Naturally fluorescent anthracycline antibiotic, anticancer drug. Doxorubicin is a substrate of MRP1 which was first cloned from a DOX-resistant lung cancer cell line. Fluorescent property has been exploited for the measurement of drug efflux pump activities as well as resolving the important question of intracellular localization of various multidrug resistance proteins and the role of subcellular organelles (Golgi and lysosome) in the sequestration of drugs and its implication in drug resistant phenotypes.

法律信息

Adriamycin 为以下机构的注册商标： RDF Pharmacia & Upjohn S.P.A.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Sigma 放线菌素 D

产品编号 A9415 | CAS号 50-76-0 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation with wide-ranging applications ranging from a selection agent in cell culture, use in studies of suppressing HIV-replication and programmed cell death of PC12 cells, and as an antibiotic in treatment of various malignant neoplasms, including Wilm′s tumour and the sarcomas. Recommended for us in cell culture applications at 1μg/mL.

Caution

This product is hygroscopic and light-sensitive. If stored protected from light and moisture at 2-8°C, it remains unchanged, by HPLC testing, for at least 15 months. Unused dilute solutions of Actinomycin D are extremely sensitive to light and tend to adsorb to plastic and glass and should be discarded. However, frozen aliquots of concentrated stock solution are stable for at least a month.

包装：2, 5, 10, 25 mg in glass bottle

Preparation Note

Actinomycin D is sold as red, shiny crystals and is soluble in acetonitrilie or acetone at 10 mg/mL and in DMSO at a minimum of 1 mg/mL. Some references also show slight solubility in water of 0.5 mg/mL.

Biochem/physiol Actions

Actinomycin D inhibits the proliferation of cells by forming a stable complex with double-stranded DNA, inhibiting DNA-primed RNA synthesis and causing single-stranded breaks in DNA. It has been shown to be an inhibitor of the minus-strand transfer step in reverse transcriptase.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Sigma 二甲基亚砜DMSO

产品编号 D2650 | CAS号 67-68-5 | SIGMA

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

DMSO 是一种用于化学反应（如聚合酶链式反应 (PCR)）的极性非质子溶剂，也可作为冷冻保护玻璃化剂用于保存细胞、组织和器官。DMSO 可在细胞冷冻培养基中用于保护细胞免受冰晶导致的机械损伤。它可用于原代、继代培养和重组的异倍体和杂交瘤细胞系、胚胎干细胞 (ESC) 以及造血干细胞的冻存。DMSO 常与 BSA 或胎牛血清 (FBS) 一同使用。

Caution

容易过度冷却，并在室温下缓慢融化固化的产品放在室温下可以再次液化，对其本身无影响。

Other Notes

This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.

包装:5mL 和 10mL 火焰封口安瓿瓶包装，100mL 棕色瓶包装

sigma 醋氨酚BioXtra

产品编号 A7085 | CAS号 103-90-2 | SIGMA-ALDRICH

Application：镇痛剂。

包装：1 kg in glass bottle，100, 500 g in glass bottle

sigma-白蛋白 来源于牛血清

产品编号 A3803 | CAS号 9048-46-8 | SIGMA

包装：10, 50, 100 g in poly bottle

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn^[] method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term “Fraction V” has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

采用热休克分级分离法制备。经过进一步纯化以降低脂肪酸含量。

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

产品编号 A8806 | CAS号 9048-46-8 | SIGMA

包装：1, 5 g in poly bottle

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn^[] method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term “Fraction V” has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

通常称为 Cohn fraction V；本品采用改进的 Cohn 氏冷乙醇分级分离法制备。

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.^[]

Protocols & Applications

Select the right albumin for your application with the Albumin Selection Guide

Sigma特殊产品Thiopropyl–Sepharose® 6B

产品编号 T8387 | CAS号 68517-67-9 | SIGMA

包装：1, 5, 15 g in poly bottle

Physical form

Lyophilized powder stabilized with lactose and dextran

Application

Thiopropyl Sepharose^® 6B is used in protein chromatography, affinity chromatography and hydrophobic interactions. Thiopropyl Sepharose^® 6B has been used for the removal of inhibitors from PCR extracts from evidence collected for law enforcement murder investigations. Thiopropyl Sepharose^® 6B has also been used to describe a method for the investigation of functional properties of distinct domains of viral thiol proteins, including the influenza virus membrane M1 protein.

法律信息

Sepharose is a registered trademark of GE Healthcare