日水 (Nissui) 快速测试皿使用说明书
NISSUI制药公司提供全套食品和环境卫生检测产品，适合于食品相关机构、医院、药品制造企业、实验室及其他药品相关机构。 用于食品卫生检测、环境控制、食品毒性检测、水中大肠杆菌检测、食品过敏性检测、食品生产环境的清洁检测、菌落计数及控制系统。
本品用于微生物计数及检测，简易性相当于3M公司的Petrifilm 微生物检测纸片，但其有具有平板的性质，检测灵敏性准确性优于Petrifilm
原理：一旦液体样品滴入，样品就会自动均匀扩 散入平板，被包被固体培养基转化成凝胶
特性：
小而致密的固体培养基（已灭菌）
样品可在平板中自动均匀扩散
基于显色底物的清晰显色
与常规方法（混合稀释培养）有较好的相 关性
可挑取群落且非常容易
可常温保存，保质期在一年以上
使用：
A. Compact Dry “Nissui” TC（菌落总数计数板）
性质：该培养基由非选择性的培养基和氧化还原指示剂氧化三苯基四氮唑（TTC）组成，多数菌落生长在TC上显红色
步骤：
1、将样品稀释
2、将稀释样品加到培养基上
3、培养48小时
4、读取所有菌落
B. Compact Dry “Nissui”CF （大肠菌群计数板）
性质：大肠菌类会在培养基上显现蓝或蓝绿色的菌落，指示剂为X-GAL，除大肠菌类外的细菌被抑制，即使生长也不能形成带色菌落
步骤：
1、将样品稀释
2、将稀释样品加到培养基上
3、培养24小时
4、读取所有蓝和蓝绿色菌落
C. Compact Dry “Nissui”EC（大肠杆菌及大肠菌群计数板）
性质：培养基含有两种产色的酶底物品红-GAL和X-GLUC，大肠杆菌显蓝或蓝绿色，除大肠杆菌外的大肠菌类显红或粉红色
步骤：
1、将样品稀释
2、将稀释样品加到培养基上
3、培养48小时
4、读取所有蓝和蓝绿色菌落即为大肠杆菌，读取所有红蓝菌落总和即为大肠菌类的总数
D. Compact Dry “Nissui”SA（金黄色葡萄球菌计数板）
性质：金黄色葡萄球菌产生黄色素显现亮黄色菌落，并且用它的酶分解蛋黄的脂质-蛋白质复合物，该酶将菌落周围的外缘培养基变成浑浊（蛋黄反应）
步骤：
1、将样品稀释在
2、将稀释样品加到培养基上
3、滴入蛋黄悬浮液
4、培养48小时
5、读取外缘培养基为白色浑浊的菌落
E. Compact Dry “Nissui”VP（肠道弧菌计数板）
性质：parahaemolyticus 弧菌产生蓝或蓝绿色菌落，产物中含有一种特殊的产色物质，其他的弧菌形成白色或无色的菌落
步骤：
1、将样品稀释在
2、将稀释样品加到培养基上
3、培养过夜
4、读取蓝或蓝绿色菌落即为parahaemolyticus 弧菌
F. Compact Dry “Nissui”YM（酵母及霉菌计数板）
性质：培养基中含有X-PHOS，使得多数酵母菌显蓝色，一部分显奶油色，抗生素抑制细菌生长，霉菌形成带特征色的毛状菌落
步骤：
1、将样品稀释
2、将稀释样品加到培养基上
3、培养3-7天
4、读取蓝至奶油色菌落即为酵母菌，带特征色的毛状菌落为霉菌
G. Compact Dry “Nissui”SL（沙门氏菌计数板）
性质：样品中的沙门氏菌由三种不同的实验原理共同分辨，通过沙门氏菌赖氨酸脱羧作用，培养基碱化，培养基由蓝紫色变为黄色，产色物质作用，菌落又变成绿色，随着氢硫化物的产生，菌落边黑色。
Nissui Compact Dry 培养板
| 货号 |
品名 |
用途 |
包装 |
保质期（1-30℃） |
|
06740
|
Compact Dry TC
|
菌落总数计数板 |
40片
|
18个月
|
|
06741
|
240片
|
|||
|
06744
|
Compact Dry CF
|
大肠菌群计数板 |
40片
|
|
|
06745
|
240片
|
|||
|
06742
|
Compact Dry EC
|
大肠杆菌及大肠菌群计数板 |
40片
|
|
|
06743
|
240片
|
|||
|
06734
|
Compact Dry SA
|
金黄色葡萄球菌计数板 |
40片
|
12个月
|
|
06735
|
240片
|
|||
|
06748
|
Compact Dry VP
|
肠道弧菌计数板 |
40片
|
|
|
06749
|
240片
|
|||
|
06746
|
Compact Dry YM
|
酵母及霉菌计数板 |
40片
|
|
|
06747
|
240片
|
|||
|
06732
|
Compact Dry SL
|
沙门氏菌计数板 |
40片
|
|
|
06733
|
240片
|
日水Nissui培养基的用途：可广泛用于活体菌总体计数、细胞培养、环境检测及食品卫生等。日水Nissui培养基的优势：日水Nissui培养基质量优越，技术领先，不但突破一般品牌无法高温灭菌的限制，特别在细胞培养的后期传代体现出其他品牌无法替代的优点。日水Nissui培养基的保证：所有培养基生产原料均由纯化学品经严格控制在无菌条件下制成，绝不含任何动物性的原料，是疫苗生产和细胞培养等生物制药的佳选择。
1. Food Hygienic Test (Dry Plate Media):
| Code | Product Name | Application |
| 06732 | Compact Dry SL (40 plates) | Salmonella detection |
| 06733 | Compact Dry SL (240 plates) | Salmonella detection |
| 06734 | Compact Dry SA (40 plates) | Staphylococcus aureus |
| 06735 | Compact Dry SA (240 plates) | Staphylococcus aureus |
| 06738 | Swab kit “Nissui” Easy swab kit | Designed for Compact Dry |
| 06740 | Compact Dry TC (40 plates) | Total viable count |
| 06741 | Compact Dry TC (240 plates) | Total viable count |
| 06742 | Compact Dry EC (40 plates) | E. coli / Coliforms |
| 06743 | Compact Dry EC (240 plates) | E. coli / Coliforms |
| 06744 | Compact Dry CF (40 plates) | Coliforms |
| 06745 | Compact Dry CF (240 plates) | Coliforms |
| 06746 | Compact Dry YM (40 plates) | Yeast and Mold |
| 06747 | Compact Dry YM (240 plates) | Yeast and Mold |
| 06748 | Compact Dry VP (40 plates) | Vibrio parahaemolyticus |
| 06749 | Compact Dry VP (240 plates) | Vibrio parahaemolyticus |
2. Food Hygienic Test (Stamp Method):
| Code | Product Name | Application |
| 06050 | Food Stamp Standard Method Agar (100plates) | Total viable count |
| 06051 | Food Stamp Standard Method Agar (30 plates) | Total viable count |
| 06054 | Food Stamp TCBS Agar (100 plates) | Vibrio parahaemolyticus |
| 06055 | Food Stamp TCBS Agar (30 plates) | Vibrio parahaemolyticus |
| 06056 | Food Stamp TGSE Agar (100 plates) | Staphylococcus aureus |
| 06057 | Food Stamp TGSE Agar (30 plates) | Staphylococcus aureus |
| 06063 | Food Stamp Sabouraud Agar (100 plates) | Fungi |
| 06064 | Food Stamp Sabouraud Agar (30 plates) | Fungi |
| 06750 | Food Stamp MLCB Agar (100 plates) | Salmonella |
| 06751 | Food Stamp MLCB Agar (30 plates) | Salmonella |
| 06752 | Food Stamp Cereus Agar (100 plates) | Bacillus cereus |
| 06753 | Food Stamp Cereus Agar (30 plates) | Bacillus cereus |
| 06754 | Food Stamp Potato dextrose Agar with CP (100plates) | Food poisoning Fungi |
| 06755 | Food Stamp Potato dextrose Agar with CP (30plates) | Food poisoning Fungi |
| 06763 | Food Stamp X-GAL Agar (100 plates) | Coliforms |
| 06764 | Food Stamp X-GAL Agar (30 plates) | Coliforms |
| 06775 | Food Stamp XM-G Agar (100 plates) | E. coli / Coliforms |
| 06776 | Food Stamp XM-G Agar (30 plates) | E. coli / Coliforms |
3. Food Hygienic Test (EVANET EV20 / EVATEST)
| Code | Product Name | Application |
| 01700 | EVATEST SET (30 tests) | Staphylococcal Enterotoxin |
| 01702 | EVATEST VT 1/2 (30 tests) | EHEC Verotoxin |
| 01300 | EVANET EV20 (1 unit) | Automated Fluorescent Immunoassay System |
4. Environmental Test (for cleanliness check)
| Code | Product Name | Application |
| 09516 | Lucifelight (50 det. X 4) | For cleanliness check used ATP detection method |
| 09518 | Lucifelight Wiping Kit (200 tubes) | For cleanliness check used ATP detection method |
| 09534 | Lucifelight LF-100 (1 unit) | Instrument for Lucifelight |
5. Environmental Test (Stamp Method)
| Code | Product Name | Application |
| For Medical Environment | ||
| 06780 | Clean Stamp MSO Agar (100 plates) | For MRSA |
| 06781 | Clean Stamp MSO Agar (30 plates) | For MRSA |
| 06782 | Clean Stamp MSEY Agar (100 plates) | For Staphylococcus |
| 06783 | Clean Stamp MSEY Agar (30 plates) | For Staphylococcus |
| For Regular Environment | ||
| 06784 | Clean Stamp SCD Agar (100 plates) | For Total viable count |
| 06785 | Clean Stamp SCD Agar (30 plates) | For Total viable count |
| 06786 | Clean Stamp SCDLP Agar (100 plates) | For Total viable count containing inactivator |
| 06787 | Clean Stamp SCDLP Agar (30 plates) | For Total viable count containing inactivator |
| 06790 | Clean Stamp25 SCD Agar (100 plates) | For Total viable count |
| 06791 | Clean Stamp25 SCD Agar (30 plates) | For Total viable count |
| 06792 | Clean Stamp25 SCDLP Agar (100 plates) | For Total viable count containing inactivator |
| 06793 | Clean Stamp25 SCDLP Agar (30 plates) | For Total viable count containing inactivator |
| 06794 | Clean Stamp25 CPSB Agar (100 plates) | For Fungi |
| 06795 | Clean Stamp25 CPSB Agar (30 plates) | For Fungi |
6. E. coli / Coliforms detection from Water
| Code | Product Name | Application |
| 05608 | EC Blue 50P (100 packs for 50mL) | For E. coli / Coliforms detection medium from Water |
| 05611 | EC Blue 50 (100 bottles for 50mL) | For E. coli / Coliforms detection medium from Water |
| 05613 | EC Blue 10 (150 bottles for 10mL) | For MPN (minimum probable number) |
7. Food Allergen Detection Kit
| Code | Product Name | Application |
| 08600 | FASTKIT ELISA “Egg” Kit | For Food Allergen screening (Egg) |
| 08601 | FASTKIT ELISA “Milk” Kit | For Food Allergen screening (Milk) |
| 08602 | FASTKIT ELISA “Wheat” Kit | For Food Allergen screening (Wheat) |
| 08603 | FASTKIT ELISA “Buckwheat” | For Food Allergen screening (Buckwheat) |
| 08604 | FASTKIT ELISA “Peanut” | For Food Allergen screening (Peanut) |
8. Colony counting
| Code | Product Name | Application |
| 01504 | NeQCS (1unit) | For automated colony counting |
9. Culture Media (dehydrated)
| Code | Product Name | Application |
| For Salmonella | ||
| 05002 | EEM Broth (100g) | Enrichment broth for Salmonella |
| 05020 | SS Agar (20L) | Isolation of Salmonella |
| 05021 | SS Agar (4L) | Isolation of Salmonella |
| 05032 | SS Agar with Sucrose (280g) | Isolation of Salmonella |
| 05033 | SS Agar with Sucrose (20L) | Isolation of Salmonella |
| 05131 | Buffered Peptone Water (BPW) (300g) | Enrichment broth for Salmonella |
| For Staphylococcus | ||
| 05234 | Staphylococcus Medium No. 110 (300g) | Isolation of Staphylococcus |
| 05236 | Mannitol Salt Agar (300g) | Isolation of Staphylococcus |
| For E. coli / Coliforms | ||
| 05036 | MacConkey Agar (300g) | Isolation of Enteric bacteria |
| 05607 | Blue Light Broth (300g) | Rapid detection of E. coli and Coliforms from Water and Foods |
| 05639 | Lauryl Sulfate MUG Broth (300g) | Rapid detection of E. coli and Coliforms from Water and Foods |
| 05642 | X-GAL Agar (300g) | Chromogenic enzyme substrate agar for Coliform isolation |
| 05643 | MacConkey Sorbitol Agar (300g) | Isolation for E. coli O157 |
| 05647 | XM-G Agar (300g) | Chromogenic enzyme substrate agar for E. coli and Coliforms |
| For Bacillus cereus | ||
| 05282 | NGKG Agar Base (300g) | Selective isolation of B. cereus from Foods |
| For Enterococci | ||
| 05679 | EF Agar Base (100g) | Isolation of Enterococci from Water, Milk and Meat products |
| For general use | ||
| 05503 | Heart Infusion Agar (300g) | Isolation for many kind of bacteria |
| 05505 | Heart Infusion Broth (100g) | Isolation for many kind of bacteria |
| 05506 | Brain Heart Infusion Agar (300g) | Isolation for many kind of bacteria |
| 05508 | Brain Heart Infusion Broth (300g) | Isolation for many kind of bacteria |
| 05516 | Trypto-Soya Agar (SCD Agar) (300g) | Isolation for many kind of bacteria |
| 05533 | Mueller-Hinton Agar-N (300g) | Isolation for many kind of bacteria |
| For Yeast and Mold | ||
| 05701 | Sabouraud Agar (300g) | Isolation of Yeast and Mold |
| 05703 | Candida GE Agar (100g) | Isolation for Candida |
| For Anaerobes | ||
| 05426 | GAM Agar, Modified (300g) | Isolation for Anaerobic bacteria |
| 05433 | GAM Broth, Modified (100g) | Isolation for Anaerobic bacteria |
| 05440 | Bacteroides Agar (100g) | Differentiation and isolation for Bacteroides |
| 05441 | FM Agar (100g) | Differentiation and isolation for Fusobacterium |
| 06593 | Anaero Mate-P (50 sets for 100 plates) | Simple bag for Anaerobic culture |
| 06594 | Anaero Mate-J (50 sets for 500 plates) | Simple bag for Anaerobic culture |
10. Identification Test Kit
| Code | Product Name | Application |
| 06626 | ID Test EB-20 (25 tests) | Identification test for Enterobacteriaceae |
| 06629 | ID Test NF-18 (25 tests) | Identification test for Gram Negative Non-fermenters |
| 06637 | ID Test SP-18 (25 tests) | Identification test for Staphylococcus |