BD BioCoat-美国BD BioCoat Matrigel基底膜/基质胶

BD BioCoat-美国BD BioCoat Matrigel基底膜/基质胶

产品介绍:

BD BioCoat™表面是在无血清或含血清培养基中强化细胞贴壁及各种原代细胞和传代细胞生长的理想选择。我们在薄膜涂层技术方法具有丰富的经验,可提供高度*且具生物功能的预涂层,并采用严格的质量控制体系,以满足您的应用需求。

可无需稀释直接包被培养器皿表面,包被厚度为0.5mm(薄层,50ul/cm2)或1.0mm(厚层,150-200ul/cm2)。也可无血清培养基稀释后包被,据细胞类型和培养目的确定相应的Matrigel浓度。所有用品在使用前需冰浴,必须用预冷的吸头及小管操作Matrigel。

美国康宁BD BioCoat Matrigel基底膜/基质胶

美国BD BioCoat Matrigel基底膜/基质胶

Corning® Biocoat基底膜/基质胶
本系列专业提供各种细胞外基质蛋白、粘附分子、细胞因子和血清添加剂,该系列产品在Falcon多孔培养板、培养皿、培养瓶、培养玻片和盖玻片的表面预包被不同成分的细胞外基质和粘附分子,为细胞提供更接近体内的生长环境。主要产品有Matrigel基质胶,collagenI, collagen II, collagen III, collagen IV, collagen V, collagen VI, Fibronectin, Laminin,,PDL,玻连蛋白等。同时还提供肿瘤侵袭系统,血管生成系统,内皮细胞侵袭迁移系统,Caco-2检测系统等。
Corning® Mtrigel®基质胶特征:
●提供广泛的动物源、人源和合成产品,满足多种细胞培养体系的需求。
● 用重组基底膜、纯化蛋白或合成肽水凝胶,确保3D细胞培养。
● Corning® Mtrigel®基质和Corning® PuraMatrixTM 肽水凝胶支持体内研究,如细胞移植。
● 人胚胎干细胞研究人员使用Corning® Mtrigel® hESC合格基质时可节省时间。
● 大范围质控检测,确保高质量产品。

美国BD BioCoat Matrigel基底膜/基质胶

订购信息:

货号 名  称 规格
356234 Matrigel基底膜 5ml
354234 10ml
356235 5*10ml
特点 蛋白浓度9-12mg/ml,含酚红。用于一般的细胞培养和分化,肿瘤迁移类实验。
354248 Matrigel基底膜,高浓度(HC) 10ml
特点 蛋白浓度18-22mg/ml,适用研究血管生成、肿瘤细胞迁移、体内肿瘤模型建立等。
356237 Matrigel基底膜,无酚红 10ml
特点 不含酚红,适用于荧光检测或显色反应等分析,消除酚红指示剂的干扰。
356230 Matrigel基底膜,低生长 5ml
354230 因子(GFR) 10ml
特点 排除生长因子干扰。适用对成分要求严格的实验,如信号通路和细胞因子研究等。
356231 Matrigel基底膜,GFR,无酚红 10ml
354263 Matrigel基底膜,GFR,HC 10ml
354262 Matrigel基底膜,无酚红,HC 10ml
354277 Matrigel HESC-Qualified(人胚胎干细胞) 5ml

其它规格

产品名称 来源 规格 货号
Corning® Mtrigel®基质 EHS小鼠肿瘤 5ml 356234
EHS小鼠肿瘤 10ml 354234
EHS小鼠肿瘤

肿瘤

 

50ml(5×10) 356235
Corning® Mtrigel® 基质,高浓度 EHS小鼠肿瘤

 

10ml 354248
Corning® Mtrigel® 基质,无酚红 EHS小鼠肿瘤

 

10ml 356237
Corning® Mtrigel® 基质,高浓度,无酚红 EHS小鼠肿瘤 10ml 354262
Corning® Mtrigel® 基质,生长因子减少 EHS小鼠

 

5ml 356230
Corning® Mtrigel® 基质,生长因子减少 EHS小鼠肿瘤 10ml 354230
Corning® Mtrigel® 基质,高浓度,生长因子减少 EHS小鼠肿瘤

 

10ml 354263
Corning® Mtrigel® hESC合格基质 EHS小鼠肿瘤 5ml 354277
Corning® Mtrigel® 基质,无酚红,生长因子减少 EHS小鼠肿瘤

 

10ml 356231

胶原蛋白

产品名称 来源 规格 货号
I型胶原 人胎盘 0.25mg 354243
鼠尾腱 100mg 354236
鼠尾腱 1g(10×100mg) 356236
I型胶原,高浓度 鼠尾腱 100mg 354249
III型胶原 人胎盘 0.25mg 354244
IV型胶原 人胎盘 0.25mg 354245
人胎盘 500μg 354261
EHSlathrytic小鼠肿瘤 1mg 354233
EHSlathrytic小鼠肿瘤 10mg(10×1mg) 356233
V型胶原 人胎盘 0.25mg 354246

糖蛋白

产品名称 来源 规格 货号
纤粘连蛋白 人胎盘 1mg 354008
人胎盘 5mg 356008
人胎盘 25mg(5×5mg) 356009
层粘连蛋白 EHS小鼠肿瘤 1mg 354232
超纯层粘连蛋白 EHS小鼠肿瘤 1mg 354239
层粘连蛋白/巢蛋白复合物,高浓度 EHS小鼠肿瘤 10.5mg 354259
骨桥蛋白 人乳汁 50μg

 

354256
玻连蛋白 人胎盘 0.25mg 354238

附着因子

产品名称 来源 规格 货号
多聚赖氨酸 人工合成 20mg 354210
Corning®PuraMatrixTM肽水凝胶 人工合成 5ml 354250
Corning® Cell-TakTM细胞和组织附着因子 紫贻贝分泌的多酚类蛋白 1mg 354240
Corning® Cell-TakTM细胞和组织附着因子 紫贻贝分泌的多酚类蛋白 5mg 354241
Corning® Cell-TakTM细胞和组织附着因子 紫贻贝分泌的多酚类蛋白 (10mg)2×5mg 354242

细胞分离/消化试剂

产品名称 物种 规格 货号
中性蛋白酶 来源于多粘芽孢杆菌的金属蛋白酶 100ml 354253
细胞消化溶液 非酶类溶液 100ml 354235

BD PAXgene全血RNA采集管(762165 )

BD  PAXgene全血RNA采集管

货号:762165

品牌:BD

描述

PAXgene® Blood RNA Tube旨在立即稳定细胞内RNA,从而产生准确且可重复的基因表达数据。它是PAXgene血液RNA系统的一部分,集成了全血采集、细胞内RNA稳定和RNA纯化的关键步骤。

PAXgene Blood RNA Tube 含有一种专有试剂,用于在采集后立即稳定细胞内 RNA。细胞内RNA在室温下可稳定长达三天,在2-8°C下可稳定5天。 PAXgene Tubes可在-20°C至-70°C的温度下冷冻,以便长期储存。

PAXgene 血液 RNA 系统是首款用于收集、储存和运输血液并在封闭管中稳定细胞内 RNA 的 IVD 产品,随后从全血中分离和纯化细胞内 RNA,用于分子诊断测试中的 RT-PCR。PAXgene 血液 RNA 系统的性能特征已通过 FOS 和 IL1B 基因转录本确定。

 

管材

塑胶

管尺寸

16×100 毫米

绘制量

2.5毫升

闭合类型

BD血脂™

闭合颜色

标签

细胞分离器

添加剂

6.9 mL 专有 RNA 稳定添加剂

灭菌

辐射 , 10-6 SAL , ISO11137-1

制备和储存

将未使用的PAXgene血RNA管储存在4−25°C下。允许的偏移温度最高可达 40°C。


1、用途
PAXgene 血液 RNA 系统由采血管(PAXgene 血液 RNA 管)和核酸纯化套件(PAXgene 血液 RNA 套件) 组成。 它适用于在分子诊断测试使用的逆转录聚合酶链反应中采集、存储和输送血液,在封闭管中稳定细胞内RNA(核糖核酸),随后从全血中分离和纯化细胞内 RNA。

2、说明
在研究细胞内 RNA 使用的许多分子测试中,采集全血是第一步。 这类测试中的最大挑战是细胞内 RNA
不稳定,在采血后几小时内迅速降解。 此外,通过基因诱导过程,某些种类的 RNA 采血后在体外增
加。 体外 RNA 降解和基因诱导均会导致体内基因转录物相对数量估计过低或过高。 PAXgene 血液
RNA 管含有一种附加剂,通过减少体外 RNA 降解和尽量减少基因诱导,可稳定体内基因转录谱。 与
PAXgene 血液 RNA 套件配套使用时,PAXgene 血液 RNA 管可以实现准确的基因转录物检测和定量。

 

  1. 标本采集和分析的准备工作

BD  PAXgene全血RNA采集管(762165 )BD  PAXgene全血RNA采集管(762165 )

BD马铃薯葡萄糖琼脂PDA(213400)

BD马铃薯葡萄糖琼脂PDA

货号:213400

规格:500g

品牌:BD Difco

BD马铃薯葡萄糖琼脂PDA(213400)

马铃薯葡萄糖琼脂  

英文名:  Potato Dextrose Agar

规格:500G,每39g配1L培养基

产地:美国

厂家:BD Difco

货号:213400

用于培养酵母菌和霉菌的基础培养基,符合USP/EP/JP等药典标准

213400配方:(1L中的含量):

Potato Starch (From Infusion) 4.0g

Dextrose                                 20.0g

Agar                                       15.0g

pH 5.6±0.2

BD 成品平板 BD成品培养基

BD 成品平板 BD成品培养基

BD BBL plated AGAR

Plated Media by BBL and Difco (BD Becton Dickinson). Chromagar Plates, Mueller Hinton Agar Plates,  MacConkey Agar Plates, Salmonella Agar Plates, Trypticase Soy Agar Plates, Potato Dextrose Agar Plates

Item No. BBL DIFCO Plated Media
292773 PLATE 150MM STERILE SAB DEX AG 3X WRAP
295964 PLATE 7H10 // 7H10 20 EA
221870 PLATE 7H11 AGAR DEEP FILL 10 EA
292211 PLATE A7 AGAR MODIFIED 60MM 10 EA
215082 PLATE AGAR MEDIUM F VRBA GLUC 100MM 10EA
299711 PLATE AGAR RODAC 20 EA
215150 PLATE AK AGAR 2 SPORULATING 100MM 10 EA
297041 PLATE ANAEROBE CNA聽聽5% SB//ANA LSB 20 EA
297165 PLATE ANAEROBE CNA SB AGAR 20 EA
297790 PLATE ANAEROBE NEOMYCIN 5% SB 20 EA
215107 PLATE ANTIBIOTIC MED 11 20X100 MM 70 EA
297214 PLATE BAIRD PARKER AGAR 20 EA
297725 PLATE BAIRD-PARKER AGAR 100 EA
221836 PLATE BBE 10 EA
254093 PLATE BBL CHROMAGAR CANDIDA 90MM 20
254102 PLATE BBL CHROMAGAR ORIENTATION 20 EACH
221808 PLATE BCYE AGAR 10 EA
215102 PLATE BCYE AGAR 100MM 100 EA
297881 PLATE BCYE DIFFERENTIAL AGAR 10 EA
297878 PLATE BCYE SELECTIVE AGAR CCVC 10 EA
297879 PLATE BCYE SELECTIVE AGAR PAC 10 EA
297880 PLATE BCYE SELECTIVE AGAR PAV 10 EA
221842 PLATE BH CC AGAR 10% SB W GENT 10 EA
221843 PLATE BHI 10% SB 20 EA
221841 PLATE BHI 10% SB W GENT & CHLOR 20 EA
221570 PLATE BHI AGAR DEEP FILL 100 EA
221569 PLATE BHI AGAR DEEP FILL 20 EA
296261 PLATE BHI CC AGAR 20 EA
296178 PLATE BHI CC W/BLD 20 EA
296097 PLATE BHI SB W/PEN-STREP 20 EA
297250 PLATE BI 7H11 & 7H11 SELECTIVE 20 EA
297260 PLATE BI BBE & CDC ANA LAKED SB & KV 100
297022 PLATE BI BBE & CDC ANA LAKED SB & KV 20
215139 PLATE BI BEA BLOOD AG BASE SALT 100 EA
297646 PLATE BI CDC ANA BL & CDC ANA BL PEA
221623 PLATE BI CHOC II & MTM II 20 EA
222239 PLATE BI CHROMAGAR ORIENT/TSA II SB 100
221941 PLATE BI COL CNA 5% SB & EMB HHT 20 EA
299818 PLATE BI COL CNA SB & COL CNA SB 100 EA
295618 PLATE BI COL CNA SB & LEMB 100 EA
295646 PLATE BI HE & XLD 20 EA
295969 PLATE BI LEMB & MAC II 100 EA
298292 PLATE BI MB 7H10 & 7H11 SELECTIVE 100 EA
297426 PLATE BI SALMONELLA SHIGELLA & HEKTOEN
215211 PLATE BI SDA AND DTM MOD CHLOR 100 EA
221303 PLATE BI TSA II SB & CHOCOLATE II 100 EA
221302 PLATE BI TSA II SB & CHOCOLATE II 20 EA
214951 PLATE BI TSA II SB & CYST HEART CHOC SB
221783 PLATE BI TSA II SB & GROUP A STREP SB
221949 PLATE BI TSA II SB & MAC II W MUG 20 EA
292501 PLATE BI TSA II SB & TSA 100 EA
221292 PLATE BI TSA II SB & TSA II SB 20 EA
293036 PLATE BI XLD & BRILLIANT GREEN 20 EA
292765 PLATE BI XLT4 & BG W NOVOBIOCIN 100 EA
297254 PLATE BIGGY AGAR 20 EA
221838 PLATE BILE ESCULIN AGAR 10 EA
297875 PLATE BIRD SEED AGAR 10 EA
292607 PLATE BISMUTH SULFITE AGAR
297876 PLATE BORDET GENGOU BLOOD AGAR 10 EA
295963 PLATE BRILLIANT GREEN AGAR 20 EA
292784 PLATE BRILLIANT GREEN NOVOBIOCIN 100 EA
221548 PLATE BRUCELLA 5% HORSE BLOOD 100 EA
221547 PLATE BRUCELLA 5% HORSE BLOOD 20 EA
297716 PLATE BRUCELLA 5% SB HEMIN VIT K1 100 EA
297848 PLATE BRUCELLA 5% SB HEMIN VIT K1 20 EA
297840 PLATE BRUCELLA LAKED BLOOD W KV 20 EA
297849 PLATE BRUCELLA SB W/O CYST//BRUC LKV 20
221728 PLATE CAMPY 5 ANTIMICRBICS 10% SB 100 EA
221727 PLATE CAMPY 5 ANTIMICRBICS 10% SB 20 EA
299471 PLATE CAMPY BLOOD FREE AGAR 100 EA
299470 PLATE CAMPY BLOOD FREE AGAR 20 EA
292487 PLATE CAMPY CEFEX AGAR 100 EA
215221 PLATE CAMPY CEFEX AGAR 100MM 20 EA
299614 PLATE CAMPY CSM AGAR 20 EA
297713 PLATE CAMPY CVA AGAR 100 EA
297246 PLATE CAMPY CVA AGAR 20 EA
296241 PLATE CANDIDA BCG AGAR 20 EA
292615 PLATE CASMAN AG MOD W/HORSE SERUM
297004 PLATE CDC ANA SB KV//CDC ANA SB PEA 20
299611 PLATE CDC ANA SB PEA//ANAEROBE LKV 20 EA
221734 PLATE CDC ANAEROBE 5% SB 100 EA
221733 PLATE CDC ANAEROBE 5% SB 20 EA
221736 PLATE CDC ANAEROBE 5% SB WITH KV 100 EA
221735 PLATE CDC ANAEROBE 5% SB WITH KV 20 EA
221739 PLATE CDC ANAEROBE 5% SB WITH PEA 20 EA
292987 PLATE CDC ANAEROBE BLOOD AGAR RODAC
221846 PLATE CDC ANAEROBE LB KV 20 EA
222228 PLATE CDSA
297767 PLATE CEM 20 EA
298206 PLATE CHOC II//MARTIN聽聽LEWIS MOD 100 EA
297259 PLATE CHOC W/PYRIDOXAL 100 EA
298513 PLATE CHOC//MOD MARTIN LEWIS 20 EA
221267 PLATE CHOCOLATE II AGAR 100 EA
221169 PLATE CHOCOLATE II AGAR 20 EA
221882 PLATE CHOCOLATE II AGAR 30 EA
296271 PLATE CHOCOLATE II AGAR BACITRACIN 20 EA
292381 PLATE CHOCOLATE II AGAR RODAC 20 EA
297696 PLATE CHOCOLATE II W/VANCOMYCIN 20 EA
297060 PLATE CHOCOLATE II//MARTIN LEWIS 20 EA
215085 PLATE CHROMAGAR LISTERIA 100MM 20 EA
215229 PLATE CHROMAGAR MRSA II 100MM 100 EA
215228 PLATE CHROMAGAR MRSA II 100MM 20 EA
215245 PLATE CHROMAGAR MRSA II LUO RODAC 20 EA
214984 PLATE CHROMAGAR O157 20 PACK
215081 PLATE CHROMAGAR ORIENTATION 100 EA
214983 PLATE CHROMAGAR SALMONELLA 20 PACK
214982 PLATE CHROMAGAR STAPH AUREUS 100MM 20EA
221848 PLATE CIN AGAR YERSINIA SELECT 10 EA
299579 PLATE CIN AGAR YERSINIA SELECT 100 EA
221850 PLATE CLED AGAR 10 EA
298277 PLATE CLOSTRIDIUM DIFFICILE EGG YOLK 20
292302 PLATE COL CNA MOD SB W/VANC GENT 100 EA
297413 PLATE COL CNA MOD//ENTEROCOCCOSEL 100 EA
221601 PLATE COL CNA//MAC II 100 EA
221600 PLATE COL CNA//MAC II 20 EA
215191 PLATE COLUMBIA AGAR 20 EA
221263 PLATE COLUMBIA AGAR 5% SB 100 EA
221165 PLATE COLUMBIA AGAR 5% SB 20 EA
297930 PLATE COLUMBIA AGAR 5% SB 30 EA
221929 PLATE COLUMBIA ANAEROBE SB 100 EA
221928 PLATE COLUMBIA ANAEROBE SB 20 EA
221353 PLATE COLUMBIA CNA AGAR 5% SB 100 EA
221352 PLATE COLUMBIA CNA AGAR 5% SB 20 EA
297831 PLATE COLUMBIA CNA AGAR 5% SB 30 EA
295661 PLATE COLUMBIA FILDES BACITRACIN 20 EA
297272 PLATE COLUMBIA PNA SB//MAC II 100 EA
292499 PLATE COOKE ROSE BENGAL CHLOR RODAC 20
299714 PLATE CORN MEAL AGAR 10 EA
221854 PLATE CORN MEAL AGAR POLY 80 10 EA
292427 PLATE CORN MEAL AGAR RODAC 20 EA
297794 PLATE CZAPEK-DOX AGAR 20 EA
298333 PLATE DE AGAR RODAC 100 EA
299969 PLATE DE NEUTRALIZING AGAR 100 EA
297202 PLATE DNASE AGAR METHYL GREEN 20 EA
221856 PLATE DNASE TOLUIDINE BLUE 10 EA
299636 PLATE DTM MODIFIED W CHLOR 10 EA
297873 PLATE EGG YOLK AGAR聽聽MODIFIED 10 EA
221355 PLATE EMB AGAR MODIFIED HHT 100 EA
221354 PLATE EMB AGAR MODIFIED HHT 20 EA
221265 PLATE ENDO AGAR 100 EA
221167 PLATE ENDO AGAR 20 EA
292285 PLATE ENTEROCOCCOSEL AGAR 10 EA
221493 PLATE ENTEROCOCCOSEL AGAR 100 EA
221492 PLATE ENTEROCOCCOSEL AGAR 20 EA
292979 PLATE ENTEROCOCCOSEL SPACESAVER
292234 PLATE ENTEROCOCCOSEL W VANCOMYCIN 10 EA
222201 PLATE ENTEROCOCCUS SCREEN AG QUAD 10 EA
292296 PLATE ESCULIN IRON AGAR 50MM 150 EA
298288 PLATE FLO AGAR 20 EA
221240 PLATE GC II ISOVITALEX 8 EA
292364 PLATE GC LET W/PATIENT LABEL 160 EA
297928 PLATE GC-LECT AGAR 100 EA
297715 PLATE GC-LECT AGAR 20 EA
221995 PLATE GC-LECT AGAR JEMBEC 10 EA
298243 PLATE GC-LECT PILL POCKET 100 EA
221936 PLATE GROUP A SELECT STREP SB 20 EA
221780 PLATE GROUP A SELECTVE STREP 5% SB 100
221779 PLATE GROUP A SELECTVE STREP 5% SB 20 EA
221954 PLATE HAEMOPHILUS TEST MEDIUM AGAR 8 EA
297884 PLATE HBT BILAYER MEDIUM 10 EA
297472 PLATE HEART INFUSION 5% RB 100 EA
221366 PLATE HEKTOEN ENTERIC AGAR 100 EA
221365 PLATE HEKTOEN ENTERIC AGAR 20 EA
297890 PLATE HEMO ID QUAD GROWTH FCTRS 10 EA
295914 PLATE HEMO ISO BACITRACIN 20 EA
297799 PLATE INHIBITORY MOLD 10 EA
298191 PLATE INHIBITORY MOLD 100 EA
299715 PLATE INHIBITORY MOLD CHLOR & GENT 10 EA
297800 PLATE INHIBITORY MOLD GENT 10 EA
293044 PLATE ISOL CONTACT BREWER ANA L & P80
215098 PLATE ISOL TSA LEC P80 PNASE RODAC LABEL
292901 PLATE ISOLATOR 100MM TSA W/PEN
292999 PLATE ISOLATOR SDA L P80 & CHLOR 100MM
292998 PLATE ISOLATOR SDA L P80 & CHLOR CONTACT
215167 PLATE KF STREP AGAR 50MM 30 EACH
292346 PLATE KF STREPTOCOCCAL AGAR 10 EA
298272 PLATE LB AGAR聽聽LENNOX 20 EA
292473 PLATE LB AGAR聽聽MILLER 100 EA
292394 PLATE LB AGAR MILLER AMP 100 MG 100 EA
292993 PLATE LB AGAR MILLER AMP 50 MG 100 EA
298273 PLATE LB LENNOX AMPICILLIN 20 EA
292833 PLATE LB LENNOX CARBENICILLIN 100 EA
292404 PLATE LB LENNOX KANAMYCIN 100 EA
299925 PLATE LB LENNOX KANAMYCIN 20 EA
292986 PLATE LB LENNOX W CHLORAM 100 EA
292982 PLATE LB LENNOX W KANAMYCIN 100 EA
292628 PLATE LB MILLER CARBENICILLIN 100 EA
292994 PLATE LB MILLER CHLORAMPHENICOL 100 EA
292995 PLATE LB MILLER GENTAMICIN 100 EA
292758 PLATE LB MILLER KANAMYCIN 100 EA
292795 PLATE LB MILLER W KANAMYCIN 100 EA
292838 PLATE LB MILLERS TETRACYCLINE 100 MM
297031 PLATE L-D PRESUMPTO QUAD 20 EA
221858 PLATE LECITHIN LACTOSE 10 EA
299648 PLATE LEGIONELLA SEL DGVP 100 EA
297920 PLATE LEGIONELLA SELECTIVE AGAR 10 EA
292472 PLATE LEMB W/DEXTROSE W/O LACT 20 EA
299832 PLATE LEMB W/TETRACYCLINE 10 EA
299479 PLATE LETHEEN AGAR 100 EA
292405 PLATE LETHEEN AGAR MODIFIED 100 EA
221268 PLATE LEVINE EMB AGAR 100 EA
221170 PLATE LEVINE EMB AGAR 20 EA
221883 PLATE LEVINE EMB AGAR SPACESAVER
299498 PLATE LSM AGAR 20 EA
292752 PLATE M ENDO AGAR LES
297349 PLATE MAC WITHOUT CRYSTAL VIOLET 20 EA
297064 PLATE MACCONKEY I 100 EA
215197 PLATE MACCONKEY I AGAR 20EA
221270 PLATE MACCONKEY II AGAR 100 EA
221172 PLATE MACCONKEY II AGAR 20 EA
221884 PLATE MACCONKEY II AGAR 30 EA
297953 PLATE MACCONKEY II AGAR SORBITOL 10 EA
298519 PLATE MACCONKEY II AGAR SORBITOL 100 EA
221938 PLATE MACCONKEY II AGAR WITH MUG 20 EA
292539 PLATE MACCONKEY II SPACESAVER 150 EA
299451 PLATE MAL AGAR 100 EA
214991 PLATE MALT EXTRACT AGAR 100 EA
221271 PLATE MANNITOL SALT AGAR 100 EA
221173 PLATE MANNITOL SALT AGAR 20 EA
221558 PLATE MARTIN LEWIS AGAR 100 EA
221793 PLATE MARTIN LEWIS GONO-PAK 20 EA
221804 PLATE MARTIN LEWIS JEMBEC 10 EA
299602 PLATE MARTIN LEWIS JEMBEC W/LABEL 10 EA
221557 PLATE MARTIN-LEWIS AGAR 20 EA
292298 PLATE M-E AGAR 50MM 150 EA
215163 PLATE MEI AGAR 50MM 30 EACH
215047 PLATE MEI AGAR 60x15MM 100 EA
215045 PLATE MEI AGAR 60x15MM 20 EA
298291 PLATE M-ENDO AGAR LES 20 EA
292295 PLATE M-ENDO AGAR LES 50MM 150 EA
292349 PLATE M-ENDO AGAR LES 60MM 100 EA
292297 PLATE M-FC AGAR 50MM 150 EA
215166 PLATE MFC AGAR 50MM 30 EACH
292345 PLATE M-FC AGAR 60MM 10 EA
299788 PLATE M-GREEN YEAST AND MOLD 100 EA
299617 PLATE M-HPC AGAR 100 EA
214985 PLATE MI AGAR 100 PACK
214986 PLATE MI AGAR 20 PACK
215164 PLATE MI AGAR 50MM 30 EACH
221174 PLATE MIDDLEBROOK 7H10 DEEP FILL 20 EA
215168 PLATE MOD MTEC AGAR 50MM 30 EACH
297173 PLATE MOD NEW YORK CITY MEDIUM 20 EA
215046 PLATE MODIFIED MTEC AGAR 60x15MM 100 EA
215044 PLATE MODIFIED MTEC AGAR 60x15MM 20 EA
299601 PLATE M-PA-C AGAR 20 EA
292606 PLATE MRS AGAR 100 EA
292426 PLATE MSKB AGAR 20 EA
215165 PLATE MTEC AGAR 50MM 30 EACH
215126 PLATE MTEC AGAR 60MM 20 EA
221885 PLATE MTM 30 EA
221795 PLATE MTM II GONOPAK 20 EA
221806 PLATE MTM II JEMBEC 10 EA
221994 PLATE MUELLER HINTON 150MM 8 EA
221801 PLATE MUELLER HINTON AGAR 5% SB 24 EA
221993 PLATE MUELLER HINTON AGAR 5% SB 8 EA
221176 PLATE MUELLER HINTON AGAR SB 100MM 20 EA
221802 PLATE MUELLER HINTON CHOC 24 EA
221860 PLATE MUELLER HINTON CHOCOLATE AG 20 EA
221869 PLATE MUELLER HINTON CHOCOLATE AGAR 8 EA
221275 PLATE MUELLER HINTON II AGAR 100 EA
221177 PLATE MUELLER HINTON II AGAR 20 EA
221800 PLATE MUELLER HINTON II AGAR 24 EA
292340 PLATE MYCOPHIL LOW PH & L P80 100 EA
221847 PLATE MYCOSEL AGAR DEEP FILL 20 EA
215293 PLATE MYP AGAR 100MM 100EA
221792 PLATE NEOMYCIN BLOOD 100 EA
299961 PLATE NEOMYCIN BLOOD AGAR MOD 100 EA
296137 PLATE NNA 100 EA
298309 PLATE NOCARDIA ID QUAD 10 EA
297801 PLATE NUTRIENT AGAR 10 EA
292418 PLATE NUTRIENT AGAR TTC 100 EA
299970 PLATE OFPBL AGAR 20 EA
221952 PLATE OXACILLIN SCREEN AGAR 10 EA
297755 PLATE PC AGAR 20 EA
221886 PLATE PEA W/SHEEP BLOOD SPACESAVER
297705 PLATE PEPTIC DIGEST AGAR 20 EA
221277 PLATE PHENYLETHYL ALCOHOL 5% SB 100 EA
221179 PLATE PHENYLETHYL ALCOHOL 5% SB 20 EA
292821 PLATE POTATO DEXTROSE AGAR PH 3.5 100MM
297945 PLATE POTATO DEXTROSE DEEP FILL 100 EA
296272 PLATE POTATO DEXTROSE DEEP FILL 20 EA
292362 PLATE POTATO FLAKE AG CHLOR&GENT 100 EA
298327 PLATE POTATO FLAKE AG W/CC 10 EA
298328 PLATE POTATO FLAKE AGAR 10 EA
299737 PLATE PSEUDOMONAS ISOLATION AGAR 100 EA
292800 PLATE PSEUDOSEL AGAR 60MM
297882 PLATE PSEUDOSEL AGAR CETRIMIDE 10 EA
214957 PLATE QUAD HEK & XLD & XLD & HEK 100 EA
299480 PLATE R2A AGAR 100 EA
298283 PLATE R2A AGAR 20 EA
292258 PLATE R2A AGAR 25ML 100 EA
292907 PLATE R2A AGAR 60MM 100 EA
297883 PLATE REGAN-LOWE CHARCOAL AGAR 10 EA
298326 PLATE REGAN-LOWE W/O CEPHALEXIN 10 EA
292980 PLATE REINFORCED CLOSTRIDIAL
297964 PLATE ROSE AGAR W SB 100 EA
297961 PLATE ROSE AGAR W SB 20 EA
292397 PLATE ROSE BENGAL AGAR 20 EA
215108 PLATE ROSE BENGAL AGAR RODAC SL 100EA
298192 PLATE SAB BRAIN HEART INF AG DEEP 100EA
297802 PLATE SAB BRAIN HEART INF AGAR 10 EA
297803 PLATE SAB BRAIN HEART INF CHLOR 10 EA
292908 PLATE SAB DEX AGAR RODAC
297931 PLATE SAB DEX EMMONS W CHLOR 10 EA
297474 PLATE SAB DEX EMMONS W CHLOR 100 EA
296359 PLATE SAB DEX W CHLOR GENT 20 EA
297932 PLATE SABOURAUD DEX EMMONS CC 10 EA
221867 PLATE SABOURAUD DEXT EMMONS 100 EA
221849 PLATE SABOURAUD DEXT EMMONS 20 EA
296348 PLATE SABOURAUD DEXT EMMONS GENT 20 EA
298332 PLATE SABOURAUD DEXT L&P80 20 EA
297739 PLATE SABOURAUD DEXTROSE 150MM 24 EA
221278 PLATE SABOURAUD DEXTROSE AGAR 100 EA
221180 PLATE SABOURAUD DEXTROSE AGAR 20 EA
221851 PLATE SABOURAUD DEXTROSE CHLOR 20 EA
221279 PLATE SALMONELLA SHIGELLA AGAR 100 EA
221181 PLATE SALMONELLA SHIGELLA AGAR 20 EA
221556 PLATE SCHAEDLER KV AGAR W 5% SB 100 EA
221555 PLATE SCHAEDLER KV AGAR W 5% SB 20 EA
221540 PLATE SCHAEDLER VITAMIN K1 5% SB 100 EA
221539 PLATE SCHAEDLER VITAMIN K1 5% SB 20 EA
221935 PLATE SELECT STREPTOCOCCUS 100 EA
221934 PLATE SELECT STREPTOCOCCUS 20 EA
221868 PLATE SELECTIVE 7H11 AGAR 10 EA
221183 PLATE SERUM TELLURITE AGAR 20 EA
215133 PLATE SF AGAR 100MM 100 EA
297793 PLATE SKIRROWS MEDIUM 20 EA
214995 PLATE SLANETZ AND BARTLEY MEDIUM 100 EA
222226 PLATE SMAC W CEFIXIME TELLURITE 10 EA
222227 PLATE SMAC W CEFIXIME TELLURITE 100 EA
292618 PLATE STANDARD METHODS AGAR
297030 PLATE STANDARD METHODS AGAR 20 EA
215170 PLATE STANDARD METHODS AGAR CHLOR 100 EA
292949 PLATE STANDARD METHODS AGAR HEAVY FILL
221939 PLATE STANDARD METHODS AGAR L&P80 20 EA
297404 PLATE STANDARD METHODS RODAC 20 EA
295911 PLATE STARCH AGAR BROMCRESOL PURPLE
215203 PLATE STER 100MM TSA LP80 PENASE 25ML
293015 PLATE STER CDC ANAEROBE PENASE 100 EA
292990 PLATE STER DE NEUTRAL PENASE 100MM 100EA
292228 PLATE STER DE NEUTRAL PENASE 100MM 10EA
292227 PLATE STER DE NEUTRAL PENASE RODAC 10EA
215063 PLATE STER DE NEUTRAL PNASE RODAC 100 EA
292399 PLATE STER DE NEUTRALIZING 100 EA
215281 PLATE STER DE RODAC SNAP 10 EA
215280 PLATE STER DE RODAC SNAP 100 EA
215255 PLATE STER ISOL CDC SB L P80 PENASE
292647 PLATE STER ISOL DE NEUTRALIZIN 100MM 10E
292646 PLATE STER ISOL DE NEUTRALIZIN ROD 100EA
292645 PLATE STER ISOL DE NEUTRALIZING ROD 10EA
215271 PLATE STER ISOL DE RODAC SNAP 10 EA
215270 PLATE STER ISOL SDA LP80 RODAC SNAP 10EA
292648 PLATE STER ISOL TSA L & P80 100MM 10 EA
292649 PLATE STER ISOL TSA L & P80 100MM 100 EA
293000 PLATE STER ISOL TSA L & P80 CONTACT 100E
299896 PLATE STER ISOL TSA L & P80 RODAC 10 EA
292335 PLATE STER ISOL TSA L & P80 RODAC 100 EA
215272 PLATE STER ISOL TSA LP80 PEN ROD SNAP 10
215234 PLATE STER ISOL TSA LP80 PEN RODAC SL100
215273 PLATE STER ISOL TSA LP80 PEN RODSNAP 100
222252 PLATE STER ISOL TSA LP80 RODAC SL 10 EA
222253 PLATE STER ISOL TSA LP80 RODAC SL 100 EA
215285 PLATE STER ISOL TSA LP80 RODAC SNAP 100
215284 PLATE STER ISOL TSA LP80 RODAC SNAP 10EA
215149 PLATE STER ISOL TSA PENASE 32ML 100MM
299937 PLATE STER ISOLATOR SAB DEX L P80 10 EA
292337 PLATE STER ISOLATOR SAB DEX L P80 100 EA
292653 PLATE STER ISOLATOR SDA L & P80 10 EA
292651 PLATE STER ISOLATOR TSA 10 EA
292652 PLATE STER ISOLATOR TSA 100 EA
215223 PLATE STER ISOLATOR TSA 100MM 35ML 100EA
292272 PLATE STER ISOLATOR TSA 5 EA
292650 PLATE STER ISOLATOR TSA L & P80 5 EA
214960 PLATE STER RODAC TSA W/LEC P80 & PENASE
292226 PLATE STER ROSE BENGAL PENASE 100MM 10EA
292225 PLATE STER ROSE BENGAL PENASE RODAC 10EA
299922 PLATE STER SAB DEX AGAR 10 EA
292403 PLATE STER SAB DEX AGAR 100 EA
292530 PLATE STER SAB DEX CHLORAMPHENICOL
215253 PLATE STER SDA LP80 CHLOR ROSE BEN 100MM
215252 PLATE STER SDA LP80 CHLOR ROSE BEN RODAC
215283 PLATE STER SDA LP80 RODAC SNAP 10 EA
215282 PLATE STER SDA LP80 RODAC SNAP 100 EA
215269 PLATE STER SELECT TSB AGAR LP80 RODAC SL
292482 PLATE STER TRANSPORTER TSA 100MM 20 EA
292480 PLATE STER TRANSPORTER TSA LP80 RODAC
292268 PLATE STER TSA 150MM 45 EA
221961 PLATE STER TSA L & P80 10 EA
222208 PLATE STER TSA L & P80 100 EA
292301 PLATE STER TSA L & P80 PENASE 100 EA
221234 PLATE STER TSA L & P80 PENASE RODAC 10EA
215154 PLATE STER TSA LP80 PEN RODAC SL 100 EA
215246 PLATE STER TSA LP80 PEN RODAC SL 2 RLD
215279 PLATE STER TSA LP80 PEN RODAC SNAP 10 EA
215274 PLATE STER TSA LP80 PEN RODAC SNAP 100EA
215268 PLATE STER TSA LP80 ROD BASE LABEL 100EA
215275 PLATE STER TSA LP80 RODAC SNAP 10 EA
215276 PLATE STER TSA LP80 RODAC SNAP 100 EA
292756 PLATE STERILE 100MM TSA 30ML
292762 PLATE STERILE 100MM TSA 32ML FILL
292253 PLATE STERILE CDC ANA 150 MM 5 EA
292229 PLATE STERILE CDC ANA BLOOD BASE 10 EA
222243 PLATE STERILE DE NEUTRAL RODAC SL 10 EA
222251 PLATE STERILE DE NEUTRAL RODAC SL 100 EA
292409 PLATE STERILE DE NEUTRALIZIN 100MM 100EA
222209 PLATE STERILE DE NEUTRALIZIN RODAC 100EA
292255 PLATE STERILE DE NEUTRALIZING 10 100MM
221944 PLATE STERILE DE NEUTRALIZING 10 CONTACT
221232 PLATE STERILE DE NEUTRALIZING 10 RODAC
292997 PLATE STERILE ISOL RODAC TSA L P80 & PEN
292996 PLATE STERILE ISOL TSA L P80 AND PENASE
292342 PLATE STERILE M-HPC AGAR 100 EA
292341 PLATE STERILE R2A 100MM 100 EA
215148 PLATE STERILE R2A 100MM 18ML 100 EA
292616 PLATE STERILE RODAC R2A AGAR
215106 PLATE STERILE RODAC SDA W CHLORAM 100 EA
292540 PLATE STERILE SAB BR HRT INF 100MM 10 EA
292410 PLATE STERILE SAB BR HRT INF 100MM 100EA
292636 PLATE STERILE SAB DEX AGAR 100 EA
292351 PLATE STERILE SAB DEX L & P80聽聽100 EA
221989 PLATE STERILE SAB DEX L P80 10 CONTACT
221233 PLATE STERILE SAB DEX L&P80 10 EA RODAC
221988 PLATE STERILE SAB DEXT CONTACT 10 EA
292336 PLATE STERILE SAB DEXTROSE AGAR 100MM
299977 PLATE STERILE SABOURAUD DEXT 150MM 5 EA
299939 PLATE STERILE SABOURAUD DEXT 3 BAG 10 EA
292288 PLATE STERILE SABOURAUD DEXT 65ML 5 EA
221235 PLATE STERILE SABOURAUD DEXT RODAC 10 EA
222244 PLATE STERILE SDA L&P80 RODAC SL 10 EA
215224 PLATE STERILE SDA L&P80 RODAC SL 100 EA
221236 PLATE STERILE TSA 100MM 10 EA
222205 PLATE STERILE TSA 100MM 100 EA
299938 PLATE STERILE TSA 100MM 3 BAGS 10 EA
292428 PLATE STERILE TSA 100MM 36 EA
222206 PLATE STERILE TSA 150MM 45 EA
221237 PLATE STERILE TSA 150MM 5 EA
292257 PLATE STERILE TSA 25ML 10 EA
292269 PLATE STERILE TSA 3 BAG 100 EA
292290 PLATE STERILE TSA CONTACT 10 EA
292396 PLATE STERILE TSA HEAVY FILL 100 EA
292488 PLATE STERILE TSA L&P80 100 EA
292270 PLATE STERILE TSA L&P80 3 BAG 100 EA
292273 PLATE STERILE TSA L&P80 FINGER DAB 10 EA
292305 PLATE STERILE TSA L&P80 FINGER DAB 5 EA
221987 PLATE STERILE TSA L&P80 PENASE 10 EA
221238 PLATE STERILE TSA L&P80 RODAC 10 EA
222207 PLATE STERILE TSA L&P80 RODAC 100 EA
222248 PLATE STERILE TSA L&P80 RODAC SL 10 EA
222247 PLATE STERILE TSA L&P80 RODAC SL 100 EA
215175 PLATE STERILE TSA LP80 35ML 100MM 100 EA
292271 PLATE STERILE TSA LP80 FINGER DAB 100 EA
222246 PLATE STERILE TSA LP80 PEN RODAC SL 10EA
215062 PLATE STERILE TSA PENASE 100MM 100 EA
299975 PLATE STERILE TSA RODAC 10 EA
292266 PLATE STERILE TSA RODAC 100 EA
292398 PLATE STERILE TSA SEQ NUM 100MM 100 EA
292416 PLATE STERILE TSA TOP LABEL 100MM 100 EA
221839 PLATE STERILE TSA W PENASE 100MM 10 EA
221837 PLATE STERILE TSA W PENASE 5 EA
292344 PLATE STERILE TSB W AGAR 100 EA
292343 PLATE STERILE TSB WITH AGAR 100 EA
297891 PLATE STREP ID QUAD 10 EA
299727 PLATE STREP SELECTIVE II 20 EA
221810 PLATE SXT BLOOD AGAR 100 EA
221809 PLATE SXT BLOOD AGAR 20 EA
221872 PLATE TCBS AGAR 10 EA
292760 PLATE TECH AGAR
299889 PLATE TGE AGAR 100 EA
292392 PLATE TGEA CYCLOHEXIMIDE 100 EA
221942 PLATE THAYER MARTIN W/O NYSTATIN 20 EA
221567 PLATE THAYER-MARTIN MOD MTM II聽聽20 EA
221568 PLATE THAYER-MARTIN MOD MTM II 100 EA
221282 PLATE THAYER-MARTIN SELECTIVE 100 EA
221184 PLATE THAYER-MARTIN SELECTIVE AGAR 20 EA
215025 PLATE TRI TSA & TSA II SB & MAC II
299580 PLATE TRI TSA II SB & CHOC II & MAC II
299163 PLATE TRYPTIC SOY AGAR 5% SB 100 EA
221283 PLATE TRYPTICASE SOY AGAR 100 EA
221185 PLATE TRYPTICASE SOY AGAR 20 EA
215037 PLATE TRYPTICASE SOY AGAR 25ML FILL
221372 PLATE TRYPTICASE SOY AGAR 5% HB 20 EA
221261 PLATE TRYPTICASE SOY AGAR 5% SB 100 EA
221239 PLATE TRYPTICASE SOY AGAR 5% SB 20 EA
221881 PLATE TRYPTICASE SOY AGAR 5% SB 30 EA
296347 PLATE TRYPTICASE SOY AGAR 5%SB W/CNA 100
221803 PLATE TSA 150MM 24 EA
221260 PLATE TSA II 10% SB 100 EA
214950 PLATE TSA II 5% SB & POLYMIXIN B RODAC
214952 PLATE TSA II 5% SB 150MM 24 EA
221291 PLATE TSA II SB // MAC II 100 EA
221162 PLATE TSA II SB 20 EA
215208 PLATE TSA II SB AND STREPTO 100MM 100 EA
297346 PLATE TSA II SB W/AMPICILLIN 20 EA
297457 PLATE TSA II SB W/GENTAMICIN 20 EA
221289 PLATE TSA II SB//LEMB 100 EA
221286 PLATE TSA II SB//LEMB 20 EA
221290 PLATE TSA II SB//MAC II 20 EA
297981 PLATE TSA II W 5% SB & ESCULIN 100 EA
221356 PLATE TSA II W RB 20 EA
297759 PLATE TSA II W SB 20 EA
292906 PLATE TSA L & P80 100 EA MCTA
221945 PLATE TSA L & P80 20 EA
299964 PLATE TSA L&P80 & PENASE 100 EA
292425 PLATE TSA L&P80 100 EA HEAVY FILL
221943 PLATE TSA L&P80 2 BAG 100 EA
221287 PLATE TSA L&P80 RODAC 100 EA
221288 PLATE TSA L&P80 RODAC 20 EA
222249 PLATE TSA L&P80 RODAC SL 100 EA
222242 PLATE TSA L&P80 RODAC SL 20 EA
215278 PLATE TSA L&P80 RODAC SNAP 100 EA
215277 PLATE TSA L&P80 RODAC SNAP 20 EA
292614 PLATE TSA NO PLATE LABEL
215094 PLATE TSA STREPTOMYCIN 18ML 100 EA
292978 PLATE TSA TSA BIPLATE
215171 PLATE TSA WITH YEAST EXTRACT 100MM 100EA
292423 PLATE TSAII 10% SB 150MM 24 EA
292334 PLATE TSAII 5%SB 2 BAG 100 EA
299610 PLATE TSB AGAR 100MM 100 EA
221874 PLATE V AGAR 10 EA
221875 PLATE V AGAR 100 EA
222204 PLATE VANCOMYCIN SCREEN AGAR 10 EA
215053 PLATE VIOLET RED BILE GLUC 100MM 100 EA
215198 PLATE VIOLET RED BILE GLUCOSE AGAR 20EA
221284 PLATE XLD AGAR 100 EA
221192 PLATE XLD AGAR 20 EA
221888 PLATE XLD AGAR 30 EA
292783 PLATE XLT4 AGAR
295977 PLATE YEAST NITROGEN 20 EA
297954 PLATE YEAST NITROGEN AGAR 24 EA
297886 PLATE YEP AGAR 10 EA
296191 PLATE YERSINIA SELECTIVE MEDIUM 20 EA
297245 PLATES BI CHOCOLATE II & ML 100 EA
292654 STERILE PLATE ISOLATOR 150MM SDA W/L&P

现货供应BD灭活结核杆菌(冻干粉)231141

现货供应BD灭活结核杆菌(冻干粉)231141
产品名称: BD DIFCO灭活结核杆菌231141
英文名称: M Tuberculosis H37 Ra, Desiccatedr
产品货号: 231141
产品规格: 100mg

保存与运输: 2-8 ℃

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BD Pharmingen 555899 Lysing Buffer 红细胞裂解液- BD 349202 FACS Lysing Solution 区别

BD Pharmingen 555899 Lysing Buffer 红细胞裂解液- BD 349202 FACS Lysing Solution 区别

BD 555899 Lysing Buffer 红细胞裂解液

产品品牌:BD

产品产地:美国

产品规格:100ml

用途范围:流式细胞术(常规检测)

描述

BD Pharm Lyse™ lysing solution is a buffered, concentrated (10X) ammonium chloride-based lysing reagent. When diluted to a 1X concentration and used as recommended, BD Pharm Lyse™ lyses red blood cells following monoclonal antibody staining. The lysing solution results in good light scatter separation of lymphocytes and red blood cell debris when analyzed by flow cytometry.  BD Pharm Lyse™ does not contain a fixative agent, so leukocytes remain viable after red blood cell lysis.

BD Pharm Lyse™
RUO
AB_2869057
Flow cytometry (Routinely Tested)

BD Pharm Lyse™ 裂解液是一种缓冲、浓缩 (10X) 氯化铵基裂解试剂。 当稀释至 1X 浓度并按照推荐使用时,BD Pharm Lyse™ 会在单克隆抗体染色后裂解红细胞。 当通过流式细胞仪分析时,裂解溶液导致淋巴细胞和红细胞碎片的良好光散射分离。 BD Pharm Lyse™ 不含固定剂,因此白细胞在红细胞裂解后仍可存活。

品牌:BD Pharm Lyse™
监管状态:RUO
RRID:AB_2869057
应用:流式细胞仪(常规测试)

准备和存储标题文本

Store undiluted at 4°C.

1X diluted solutions may be stored at 4° C for up to 30 days.

推荐的实验流程

Preparation of 1X lysing solution:  Dilute the 10X concentrate 1:10 with distilled water. The pH of the 1X solution should fall within the range of pH 7.1-7.4.  Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples.

Lysing procedure:

Note: The following procedure is only applied to human whole blood red blood cell lysis. Since applications vary, for other samples such as mouse spleen red blood cell lysis (lysing incubation time up to 3 minutes at 37°C), bone marrow red blood cell lysis, each investigator should optimize the condition to obtain optimal results.

1. Add 2.0 ml  of 1X lysing solution to each tube containing up to 200 µl of a whole blood plus monoclonal antibody mixture.

2. Gently vortex each tube immediately after adding the lysing solution.

3. Incubate at room temperature, protected from light, for 15 minutes.

4. Centrifuge 200 X g for 5 minutes.

5. Carefully aspirate supernatent, without disturbing pellet.

6. Add 2.0 ml 1X PBS containing 1% heat-inactivated fetal bovine serum and 0.1% sodium azide (PBS-FBS).

7. Centrifuge at 200 X g for 5 minutes.

8. Carefully aspirate supernatent, without disturbing pellet.

9. Resuspend pellet in 0.5 ml PBS-FBS or a fixative such as 2% formaldehyde for flow cytometric analysis.

Troubleshooting: Incomplete lysis may occur for several reasons: (1) The age of the specimen may affect red cell lysis, (2) The 1X lysing solution may not have been warmed to room temperature, or (3) After adding the lysing solution, the samples may not have been vortexed sufficiently. If incomplete lysis occurs (recognized by the presence of an excessive amount of visible red blood cells in the final, washed cell suspension), repeat lysing procedure. However, lysing more than two times is not recommended.

产品简介:

BD Pharm Lyse™裂解液是一种缓冲的,浓缩的(10倍)lv hua 铵基裂解试剂。当稀释到1X浓度并按推荐使用时,BD Pharm Lyse™在单克隆抗体染色后溶解红细胞。当用流式细胞术分析时,溶解液能很好的光散射分离淋巴细胞和红细胞碎片。BD Pharm Lyse™不含固定剂,所以白细胞在红细胞溶解后仍可存活。

产品应用:

流式细胞术(常规检测)

运输与储存:

保存在4°C未稀释。

1X稀释的溶液可在4°C下保存30天。

推荐的实验流程:

1X溶出液的制备:将10X浓缩液以1:10的比例用蒸馏水稀释。1X溶液的pH值应在pH 7.1-7.4范围内。必要时调整pH值。使用前将1X溶液加热至室温。100ml的10X浓缩液将产生足够溶解500个样品的1X溶液。

溶解过程:

注:以下步骤仅适用于人全血红细胞裂解。由于应用的不同,对于其他样品,如小鼠pi zang红细胞裂解(在37℃下裂解培养时间可达3分钟),gu sui红细胞裂解,每个研究人员应优化条件,以获得 结果。

  1. 每个试管中加入2.0 ml 1X溶解液,其中含有200μl全血和单克隆抗体混合物。
  2. 加入溶解液后,立即轻轻涡旋每个管。

3.室温避光孵育15分钟。

  1. 200xg离心5分钟。
  2. 小心吸出上清液,不要干扰颗粒。
  3. 加入2.0 ml 1X PBS。
  4. 200 X g离心5分钟。
  5. 小心吸出上清液,不要干扰颗粒。
  6. 用0.5 ml PBS-FBS或2%甲醛等固定剂重悬颗粒,用于流式细胞仪分析。

产品订购信息:

货号                名称                                         规格

555899    Lysing Buffer 10X Concentrate      100mL

 

BD 349202 FACS Lysing Solution

BD FACS™ lysing solution is intended for lysing red blood cells following direct immunofluorescence staining of human peripheral blood cells with monoclonal antibodies prior to flow cytometric analysis.

BD FACS lysing solution is appropriate for use with reagents such as BD Tritest™ or BD Simultest™ reagents and a suitably equipped flow cytometer. It may be used in both lyse/wash and lyse/no-wash procedures.

BD FACS™
Human
Buffered solution with <15% formaldehyde and <50% diethylene glycol.
Flow cytometry

BD FACS™ 裂解液用于在流式细胞术分析之前用单克隆抗体对人外周血细胞进行直接免疫荧光染色后裂解红细胞。

BD FACS 裂解液适用于试剂,例如 BD Tritest™ 或 BD Simultest™ 试剂和适当配备的流式细胞仪。 它可用于裂解/洗涤和裂解/免洗涤程序。

反应性:人类
存储缓冲区:含有 <15% 甲醛和 <50% 二甘醇的缓冲溶液。
应用:流式细胞仪

BD 349202  FACS Lysing Solution 溶血素 红细胞裂解液

DESCRIPTION

BD FACS™ lysing solution is intended for lysing red blood cells following direct immunofluorescence staining of human peripheral blood cells with monoclonal antibodies prior to flow cytometric analysis.

BD FACS lysing solution is appropriate for use with reagents such as BD Tritest™ or BD Simultest™ reagents and a suitably equipped flow cytometer. It may be used in both lyse/wash and lyse/no-wash procedures

PREPARATION AND STORAGE

BD FACS lysing solution (10X) is stable until the expiration date shown on the bottle label when stored as directed. Do not use this reagent if discoloration occurs or a precipitate forms.

FACS溶血素(BDIS Catalog No.349202)用于PBMCs,培养细胞与全血制备,主要有3个作用:
(1) 全血制备时用于溶解红细胞
(2) 固定外表位
(3) 辅助通透剂
     FACS溶血素使用前用无离子水1:10稀释,勿用PBS或其它缓冲液。而且FACS溶血素必须与FACS通透液一起使用,即使是培养细胞的检测。

Wheaton瓶子-WHEATON 滚瓶 110×285mm. 700cm2可用表面积

Wheaton惠顿代理
上海金畔生物提供 Wheaton玻璃瓶,Wheaton冻存瓶,Wheaton样品瓶,Wheaton摇瓶,Wheaton塑料瓶,Wheaton血清瓶,Wheaton试剂瓶,Wheaton安瓿瓶,滚瓶,Wheaton培养瓶,Wheaton培养基瓶,Wheaton诊断瓶,Wheaton锥形瓶,Wheaton烧瓶,Wheaton烧杯,杂交瓶,取样瓶,滴瓶等玻璃及其它材质产品。
110×285. 700cm2可用表面积. 带有内置密封环和聚丙烯倒液环的白色聚丙烯旋盖. 可用Wheaton透气盖

商品详情

商品名称:WHEATON 滚瓶 110×285mm. 700cm2可用表面积
商品编号:348273
Wheaton代理商:上海金畔生物
上架时间:2016-11-24 16:15:31
  • WHEATON 滚瓶 110×285mm. 700cm2可用表面积

WHEATON 滚瓶 110×285mm. 700cm2可用表面积_滚瓶_生物玻璃_玻璃制品

Wheaton瓶子-WHEATON 滚瓶 110×355mm. 940cm2可用表面积

Wheaton惠顿代理
上海金畔生物提供 Wheaton玻璃瓶,Wheaton冻存瓶,Wheaton样品瓶,Wheaton摇瓶,Wheaton塑料瓶,Wheaton血清瓶,Wheaton试剂瓶,Wheaton安瓿瓶,滚瓶,Wheaton培养瓶,Wheaton培养基瓶,Wheaton诊断瓶,Wheaton锥形瓶,Wheaton烧瓶,Wheaton烧杯,杂交瓶,取样瓶,滴瓶等玻璃及其它材质产品。
110×355. 940cm2可用表面积. 带有丁苯橡胶盖垫的黑色酚醛树脂旋盖

商品详情

商品名称:WHEATON 滚瓶 110×355mm. 940cm2可用表面积
商品编号:348524
Wheaton代理商:上海金畔生物
上架时间:2016-11-24 16:15:31
  • WHEATON 滚瓶 110×355mm. 940cm2可用表面积

WHEATON 滚瓶 110×355mm. 940cm2可用表面积_滚瓶_生物玻璃_玻璃制品

Wheaton瓶子-WHEATON Cryule带刻痕冻存安瓿瓶 1.2 mL 144个/盒

Wheaton惠顿代理
上海金畔生物提供 Wheaton玻璃瓶,Wheaton冻存瓶,Wheaton样品瓶,Wheaton摇瓶,Wheaton塑料瓶,Wheaton血清瓶,Wheaton试剂瓶,Wheaton安瓿瓶,滚瓶,Wheaton培养瓶,Wheaton培养基瓶,Wheaton诊断瓶,Wheaton锥形瓶,Wheaton烧瓶,Wheaton烧杯,杂交瓶,取样瓶,滴瓶等玻璃及其它材质产品。
用于生物样品保存. 含有刻痕. 允许低温储存. 硼硅玻璃符合USPⅠ型以及ASTMⅠ型标准. A类要求. 可用于液氮的气相中

商品详情

商品名称:WHEATON Cryule带刻痕冻存安瓿瓶 1.2 mL 144个/盒
商品编号:651483
Wheaton代理商:上海金畔生物
上架时间:2016-11-24 16:15:31

WHEATON 安瓿瓶通过密封,确保样品的纯度和样品保质期的玻璃储存容器。WHEATON 安瓿瓶是标准包装、环境应用、诊断标准、冻干以及冷冻储存的理想选择。安瓿瓶通过密封措施来防止样品受到污染。带有金环的安瓿瓶颈表明带有刻痕,可以直接打开。安瓿瓶产品线包括带刻痕的、标准的、玻璃Cryule 冻存以及Vacule 冻干安瓿瓶。    WHEATON 也可以根据客户的要求定制安瓿瓶。

  • 样品追溯性——使用WHEATON Cryule 冻存安瓿瓶

  • 玻璃安瓿瓶提供了长期储存样品的完整性,由于传统的安瓿瓶没有条形码,WHEATON 可根据客户的要求提供安瓿瓶条形码的定制。

  • 陶瓷二维码或者线性条形码可以应用于安瓿瓶,陶瓷条形码可在极端温度下使用

  • 二维码模块为矩形,用于机读扫描识别

  • 线性条形码为黑白直线型标识,用于机读识别

  • 条形码上的数字序列是唯一的,不重复

  • 当安瓿瓶和二维码一起使用时,二维码可以通过 WHEATON SingleScan 或者WHEATON PluraScan 来阅读条形码。

  • 条形码数据可简单地储存在Microsoft Excel文件中或者其他数据收集系统中。

  • WHEATON 也可以定制安瓿瓶,并提供超净以及无菌服务。

    Cryule 冻存安瓿瓶

    用于生物样品保存,允许低温储存,硼硅玻璃符合USP Ⅰ型以及ASTM E 438 Ⅰ型标准,A 类要求,可用于液氮蒸汽冷凝, 的设计允许低温储存和快速解冻

订货号

规格(mL)

直径×高度(mm)

顶开口OD(mm)

包装数量

651463

1.2

11.8×58

5

144/盒

651466

2

11.5×70

5

144/盒

    Cryule 带刻痕冻存安瓿瓶

    用于生物样品保存,带刻痕,允许低温储存,硼硅玻璃符合USP Ⅰ型以及ASTM Ⅰ型标准,A 类要求,可用于液氮蒸汽冷凝 ,的设计允许低温储存和快速解冻

订货号

规格(mL)

直径×高度(mm)

顶开口OD(mm)

包装数量

651483

1.2

11.8×58

5

144/盒

651486

2

11.5×70

5

144/盒

Vacule 冻干安瓿瓶

用于小容积冻干样品,硼硅玻璃符合USP Ⅰ型以及ASTM Ⅰ型标准,A 类要求,热封口或者塞封口( 使用Wheaton W651450 )

订货号

描述

直径x高度(mm)

顶开口OD(mm)

包装数量

W651446

2mL容量

12×86

10

567/盒

W651450

11mm灰丁基橡胶塞

144/盒

    Vacule 带刻痕冻干安瓿瓶

    用于小容积冻干样品,带刻痕易打开,硼硅玻璃符合USP Ⅰ型以及ASTM Ⅰ型标准,A 类要求,热封口

订货号

规格(mL)

直径×高度(mm)

顶开口OD(mm)

包装数量

651502

1

10×108

10

144

W651506

2

11.8×122

11.75

567

无色透明带刻痕安瓿瓶

带刻痕易打开,硼硅玻璃符合USP Ⅰ型以及ASTM Ⅰ型标准,A 类要求,顶封口或拉颈部拉伸封口

订货号

规格(mL)

直径×高度(mm)

包装数量

176772

1

10.5×67

144

176776

2

12×75

144

176779

5

16.5×84

144

176780

10

19×107

144

176782

20

22.5×130

144

    带折断点安瓿瓶

    带折断点,无需工具即可打开硼硅玻璃符合USP Ⅰ型以及ASTM Ⅰ型标准,A 类要求,颈部可拉伸或顶部密封

订货号

规格(mL)

直径×高度(mm)

包装数量

W176784A

50

27.5×170

100

    棕色带刻痕安瓿瓶

    带刻痕,硼硅玻璃符合USP Ⅰ型以及ASTM Ⅰ型标准,A 类要求,颈部可拉伸或顶部密封

订货号

规格(mL)

直径×高度(mm)

包装数量

176792

1

10.5×67

144/箱

176796

2

12×75

144/箱

176799

5

16.5×84

144/箱

标准安瓿瓶

常用于包装认证标准,硼硅玻璃符合USP Ⅰ型以及ASTM Ⅰ型标准,A 类要求背景低

订货号

规格(mL)

直径×高度(mm)

包装数量

176759

7

17×95

25

176762

20

28×100

25

一次性安瓿瓶开瓶保护套

用于防止玻璃碎片伤手,避免交叉污染

订货号

适用安瓿瓶规格

包装数量

177105

1和2 mL

144

  • WHEATON Cryule带刻痕冻存安瓿瓶 1.2 mL 144个/盒

WHEATON Cryule带刻痕冻存安瓿瓶 1.2 mL 144个/盒_冻存/冻干安瓿瓶_安瓿瓶_玻璃制品