标签归档：BD

Vector Lab代理Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

发表于由

Vector Labs代理，欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

BA-6000-1.5

SKU Unit Size Price Qty
BA-6000-1.5 1.5 mg

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

Description
Specifications
Documents
Related Products
Citations
Technical Information

Description

The biotinylated rabbit anti-sheep IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Suitable for use with goat, sheep, and bovine IgG primary antibodies 
  • Recognizes both heavy and light chains (H+L) 
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 
  • Included in the VECTASTAIN® ABC kits 
  • Supplied in solution

Specifications

More Information
Unit Size 1.5 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin.
Recommended Usage The recommended concentration range for use is 2-10 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Important Note:The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of anti-sheep IgG with bovine immunoglobulins that may be present.If this antibody is to be used in these applications, blocking with Animal-Free Blocker™ solution (Cat. No. SP-5030) is recommended. Note:This product is at the same concentration as that provided in the VECTASTAIN® ABC kits.
Target Species Sheep
Conjugate Biotinylated
Host Species Rabbit
Format Concentrate

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

Fluorescein Avidin DCS, Cell Sorting Grade Catalog #: A-2011-1

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

Texas Red® Avidin DCS, Cell Sorting Grade Catalog #: A-2016-1

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

VECTASTAIN® ABC-AP Kit, Alkaline Phosphatase (Standard) Catalog #: AK-5000

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

VECTASTAIN® ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-4000

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

VECTASTAIN® Elite ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-6100

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight® 488 Catalog #: SA-5488-1

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight® 549 Catalog #: SA-5549-1

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight® 594 Catalog #: SA-5594-1

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight® 649 Catalog #: SA-5649-1

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

Streptavidin, Peroxidase, R.T.U. Catalog #: SA-5704-100

Citations

Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated Powered by Bioz See more details on Bioz

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. 

With some exceptions the recommended dilution for most applications is 1:200.

The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker® solution (Cat. No. SP-5030) is recommended.

Home All Products Biotinylated Secondary Antibodies Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated

Vector Lab代理Goat Anti-Cat IgG Antibody (H+L), Biotinylated

发表于由

Vector Labs代理，欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

Goat Anti-Cat IgG Antibody (H+L), Biotinylated

BA-9000-1.5

SKU Unit Size Price Qty
BA-9000-1.5 1.5 mg

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Goat Anti-Cat IgG Antibody (H+L), Biotinylated

Description
Specifications
Documents
Related Products
Citations
Technical Information

Description

Biotinylated Goat Anti-Cat IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species 
  • Recognizes both heavy and light chains (H+L) 
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 

Specifications

More Information
Unit Size 1.5 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin.
Recommended Usage The recommended concentration range for use is 2-10 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target Species Cat
Conjugate Biotinylated
Host Species Goat
Format Concentrate

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

Goat Anti-Cat IgG Antibody (H+L), Biotinylated

Fluorescein Avidin DCS, Cell Sorting Grade Catalog #: A-2011-1

Goat Anti-Cat IgG Antibody (H+L), Biotinylated

Texas Red® Avidin DCS, Cell Sorting Grade Catalog #: A-2016-1

Goat Anti-Cat IgG Antibody (H+L), Biotinylated

VECTASTAIN® ABC-AP Kit, Alkaline Phosphatase (Standard) Catalog #: AK-5000

Goat Anti-Cat IgG Antibody (H+L), Biotinylated

VECTASTAIN® ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-4000

Goat Anti-Cat IgG Antibody (H+L), Biotinylated

VECTASTAIN® Elite ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-6100

Goat Anti-Cat IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight® 488 Catalog #: SA-5488-1

Goat Anti-Cat IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight® 549 Catalog #: SA-5549-1

Goat Anti-Cat IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight® 594 Catalog #: SA-5594-1

Goat Anti-Cat IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight® 649 Catalog #: SA-5649-1

Goat Anti-Cat IgG Antibody (H+L), Biotinylated

Streptavidin, Peroxidase, R.T.U. Catalog #: SA-5704-100

Citations

Goat Anti-Cat IgG Antibody (H+L), Biotinylated Powered by Bioz See more details on Bioz

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. 

Biotinylated Goat Anti-Cat IgG (H+L) is supplied in liquid format. With some exceptions the recommended dilution for most applications is 1:200.

This antibody is also suitable for use with dog IgG primary antibodies.

Home All Products Biotinylated Secondary Antibodies Goat Anti-Cat IgG Antibody (H+L), Biotinylated

Vector Lab代理Goat Anti-Hamster IgG Antibody (H+L), Biotinylated

发表于由

Vector Labs代理，欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

Goat Anti-Hamster IgG Antibody (H+L), Biotinylated

BA-9100-1.5

SKU Unit Size Price Qty
BA-9100-1.5 1.5 mg

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Goat Anti-Hamster IgG Antibody (H+L), Biotinylated

Description
Specifications
Documents
Related Products
Citations
Technical Information

Description

Biotinylated Goat Anti-Hamster IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. This antibody can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Recognizes both heavy and light chains (H+L) 
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 
  • Supplied in solution
  • This anti-hamster IgG antibody conjugate recognizes IgG from both Syrian and Armenian hamsters

Specifications

More Information
Unit Size 1.5 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin.
Recommended Usage The recommended concentration range for use is 2-10 µg/ml.If this biotinylated antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target Species Hamster
Conjugate Biotinylated
Host Species Goat
Format Concentrate

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

Goat Anti-Hamster IgG Antibody (H+L), Biotinylated

Fluorescein Avidin DCS, Cell Sorting Grade Catalog #: A-2011-1

Goat Anti-Hamster IgG Antibody (H+L), Biotinylated

Texas Red® Avidin DCS, Cell Sorting Grade Catalog #: A-2016-1

Goat Anti-Hamster IgG Antibody (H+L), Biotinylated

VECTASTAIN® ABC-AP Kit, Alkaline Phosphatase (Standard) Catalog #: AK-5000

Goat Anti-Hamster IgG Antibody (H+L), Biotinylated

VECTASTAIN® ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-4000

Goat Anti-Hamster IgG Antibody (H+L), Biotinylated

VECTASTAIN® Elite ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-6100

Goat Anti-Hamster IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight® 488 Catalog #: SA-5488-1

Goat Anti-Hamster IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight® 549 Catalog #: SA-5549-1

Goat Anti-Hamster IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight® 594 Catalog #: SA-5594-1

Goat Anti-Hamster IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight® 649 Catalog #: SA-5649-1

Goat Anti-Hamster IgG Antibody (H+L), Biotinylated

Streptavidin, Peroxidase, R.T.U. Catalog #: SA-5704-100

Citations

Goat Anti-Hamster IgG Antibody (H+L), Biotinylated Powered by Bioz See more details on Bioz

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. 

With some exceptions the recommended dilution for most applications is 1:200.

 

 

Home All Products Biotinylated Secondary Antibodies Goat Anti-Hamster IgG Antibody (H+L), Biotinylated

Vector Lab代理Goat Anti-Rat IgG Antibody (H+L), Biotinylated

发表于由

Vector Labs代理，欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

Goat Anti-Rat IgG Antibody (H+L), Biotinylated

BA-9400-1.5

SKU Unit Size Price Qty
BA-9400-1.5 1.5 mg

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Goat Anti-Rat IgG Antibody (H+L), Biotinylated

Description
Specifications
Documents
Related Products
Citations
Technical Information

Description

Biotinylated Goat Anti-Rat IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species.   (For mouse tissue applications, please see anti-rat, mouse adsorbed, secondary antibodies.) 
  • Recognizes both heavy and light chains (H+L) 
  • Supplied in solution 

Specifications

More Information
Unit Size 1.5 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin.
Recommended Usage The recommended concentration range for use is 2-10 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. NOTE: This product is raised in goat and is not the same as that contained in the VECTASTAIN ABC Kits for the detection of rat IgG which is raised in rabbit.
Target Species Rat
Conjugate Biotinylated
Host Species Goat
Format Concentrate

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

Goat Anti-Rat IgG Antibody (H+L), Biotinylated

Fluorescein Avidin DCS, Cell Sorting Grade Catalog #: A-2011-1

Goat Anti-Rat IgG Antibody (H+L), Biotinylated

Texas Red® Avidin DCS, Cell Sorting Grade Catalog #: A-2016-1

Goat Anti-Rat IgG Antibody (H+L), Biotinylated

VECTASTAIN® ABC-AP Kit, Alkaline Phosphatase (Standard) Catalog #: AK-5000

Goat Anti-Rat IgG Antibody (H+L), Biotinylated

VECTASTAIN® ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-4000

Goat Anti-Rat IgG Antibody (H+L), Biotinylated

VECTASTAIN® Elite ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-6100

Goat Anti-Rat IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight® 488 Catalog #: SA-5488-1

Goat Anti-Rat IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight® 549 Catalog #: SA-5549-1

Goat Anti-Rat IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight® 594 Catalog #: SA-5594-1

Goat Anti-Rat IgG Antibody (H+L), Biotinylated

Streptavidin, DyLight® 649 Catalog #: SA-5649-1

Goat Anti-Rat IgG Antibody (H+L), Biotinylated

Streptavidin, Peroxidase, R.T.U. Catalog #: SA-5704-100

Citations

Goat Anti-Rat IgG Antibody (H+L), Biotinylated Powered by Bioz See more details on Bioz

Technical Information

Vector Laboratories affinity-purified antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. 

With some exceptions the recommended dilution for most applications is 1:200.

Home All Products Biotinylated Secondary Antibodies Goat Anti-Rat IgG Antibody (H+L), Biotinylated

Vector Lab代理Horse Anti-Rabbit IgG Antibody (H+L), DyLight® 488

发表于由

Vector Labs代理，欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

Horse Anti-Rabbit IgG Antibody (H+L), DyLight® 488

DI-1088-1.5

SKU Unit Size Price Qty
DI-1088-1.5 1.5 mg

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Horse Anti-Rabbit IgG Antibody (H+L), DyLight® 488

Description
Specifications
Documents
Related Products
Citations
Technical Information

Description

DyLight 488 Horse Anti-Rabbit IgG Antibody can be used for tissue staining and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species 
  • Recognizes both heavy and light chains (H+L) 
  • Optimally labeled with DyLight® 488 to provide the brightest label for fluorescence microscopy 
  • Supplied in solution 
  • Excitation:  493 nm 
  • Emission: 518 nm 
  • Color: Green

Specifications

More Information
Unit Size 1.5 mg
Applications Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission 518 nm
Maximum Excitation 493 nm
Recommended Usage Recommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target Species Rabbit
Conjugate DyLight 488
Color of Fluorescence Green
Host Species Horse
Format Concentrate

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

Horse Anti-Rabbit IgG Antibody (H+L), DyLight® 488

VECTASHIELD® Antifade Mounting Medium Catalog #: H-1000-10

Horse Anti-Rabbit IgG Antibody (H+L), DyLight® 488

VECTASHIELD® Antifade Mounting Medium with DAPI Catalog #: H-1200-10

Horse Anti-Rabbit IgG Antibody (H+L), DyLight® 488

VECTASHIELD® Antifade Mounting Medium with Propidium Iodide (PI) Catalog #: H-1300-10

Horse Anti-Rabbit IgG Antibody (H+L), DyLight® 488

VECTASHIELD® HardSet™ Antifade Mounting Medium Catalog #: H-1400-10

Horse Anti-Rabbit IgG Antibody (H+L), DyLight® 488

VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10

Horse Anti-Rabbit IgG Antibody (H+L), DyLight® 488

VECTASHIELD® HardSet™ Antifade Mounting Medium with Phalloidin Catalog #: H-1600-10

Citations

Horse Anti-Rabbit IgG Antibody (H+L), DyLight® 488 Powered by Bioz See more details on Bioz

Technical Information

The horse anti-rabbit Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis.

DyLight® fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.  

DyLight dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight dyes can be applied as single labels or in combination with other DyLight dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The Dylight dyes currently offered are DyLight 488 (green), DyLight 549 (orange), DyLight 594 (red), and DyLight 649 (far red).

 

Horse Anti-Rabbit IgG Antibody (H+L), DyLight® 488

Horse Anti-Rabbit IgG Antibody (H+L), DyLight® 488

Home All Products Fluorophore-Conjugated Secondary Antibodies Horse Anti-Rabbit IgG Antibody (H+L), DyLight® 488

Vector Lab代理Goat Anti-Rabbit IgG Antibody (H+L), DyLight® 594

发表于由

Vector Labs代理，欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

Goat Anti-Rabbit IgG Antibody (H+L), DyLight® 594

DI-1594-1.5

SKU Unit Size Price Qty
DI-1594-1.5 1.5 mg

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Goat Anti-Rabbit IgG Antibody (H+L), DyLight® 594

Description
Specifications
Documents
Related Products
Citations
Technical Information

Description

DyLight 594 Goat Anti-Rabbit IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Optimally labeled with DyLight® 594 to provide the brightest label for fluorescence microscopy
  • Recognizes both heavy and light chains (H+L) 
  • Supplied in solution
  • Excitation: 592 nm
  • Emission: 617 nm
  • Color: Red

Specifications

More Information
Unit Size 1.5 mg
Applications Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission 617 nm
Maximum Excitation 592 nm
Recommended Usage Recommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target Species Rabbit
Conjugate DyLight 594
Color of Fluorescence Red
Host Species Goat
Format Concentrate

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

Goat Anti-Rabbit IgG Antibody (H+L), DyLight® 594

VECTASHIELD® Antifade Mounting Medium Catalog #: H-1000-10

Goat Anti-Rabbit IgG Antibody (H+L), DyLight® 594

VECTASHIELD® Antifade Mounting Medium with DAPI Catalog #: H-1200-10

Goat Anti-Rabbit IgG Antibody (H+L), DyLight® 594

VECTASHIELD® HardSet™ Antifade Mounting Medium Catalog #: H-1400-10

Goat Anti-Rabbit IgG Antibody (H+L), DyLight® 594

VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10

Citations

Goat Anti-Rabbit IgG Antibody (H+L), DyLight® 594 Powered by Bioz See more details on Bioz

Technical Information

The goat anti-rabbit Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. 

DyLight® fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.  

DyLight® dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight® dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight® dyes can be applied as single labels or in combination with other DyLight® dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The Dylight® dyes currently offered are DyLight® 488 (green), DyLight® 549 (orange), DyLight® 594 (red), and DyLight® 649 (far red).

 

Goat Anti-Rabbit IgG Antibody (H+L), DyLight® 594

Goat Anti-Rabbit IgG Antibody (H+L), DyLight® 594

Home All Products Fluorophore-Conjugated Secondary Antibodies Fluorophore-Conjugated Secondary Antibodies (Concentrates) Goat Anti-Rabbit IgG Antibody (H+L), DyLight® 594

Vector Lab代理Horse Anti-Mouse IgG Antibody (H+L), DyLight® 649

发表于由

Vector Labs代理，欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

Horse Anti-Mouse IgG Antibody (H+L), DyLight® 649

DI-2649-1.5

SKU Unit Size Price Qty
DI-2649-1.5 1.5 mg

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Horse Anti-Mouse IgG Antibody (H+L), DyLight® 649

Description
Specifications
Documents
Related Products
Citations
Technical Information

Description

DyLight 649 Horse Anti-Mouse IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Recognizes both heavy and light chains (H+L) 
  • Optimally labeled with DyLight® 649 to provide the brightest label for fluorescence microscopy 
  • Supplied in solution 
  • Excitation: 655 nm 
  • Emission: 670 nm 
  • Color: Far Red

Specifications

More Information
Unit Size 1.5 mg
Applications Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission 670 nm
Maximum Excitation 665 nm
Recommended Usage Recommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target Species Mouse
Conjugate DyLight 649
Color of Fluorescence Far Red
Host Species Horse
Format Concentrate

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

Horse Anti-Mouse IgG Antibody (H+L), DyLight® 649

VECTASHIELD® Antifade Mounting Medium with DAPI Catalog #: H-1200-10

Horse Anti-Mouse IgG Antibody (H+L), DyLight® 649

VECTASHIELD® Antifade Mounting Medium with Propidium Iodide (PI) Catalog #: H-1300-10

Horse Anti-Mouse IgG Antibody (H+L), DyLight® 649

VECTASHIELD® HardSet™ Antifade Mounting Medium Catalog #: H-1400-10

Horse Anti-Mouse IgG Antibody (H+L), DyLight® 649

VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10

Horse Anti-Mouse IgG Antibody (H+L), DyLight® 649

VECTASHIELD® Antifade Mounting Medium Catalog #: H-1000-10

Citations

Horse Anti-Mouse IgG Antibody (H+L), DyLight® 649 Powered by Bioz See more details on Bioz

Technical Information

The anti-mouse Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. 
 
DyLight® fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.  

DyLight® dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight® dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight® dyes can be applied as single labels or in combination with other DyLight® dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The Dylight® dyes currently offered are DyLight® 488 (green), DyLight® 549 (orange), DyLight® 594 (red), and DyLight® 649 (far red).

 

Horse Anti-Mouse IgG Antibody (H+L), DyLight® 649

Horse Anti-Mouse IgG Antibody (H+L), DyLight® 649

Home All Products Fluorophore-Conjugated Secondary Antibodies Fluorophore-Conjugated Secondary Antibodies (Concentrates) Horse Anti-Mouse IgG Antibody (H+L), DyLight® 649

Vector Lab代理VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 488 Antibody Kit

发表于由

Vector Labs代理，欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 488 Antibody Kit

DK-1488

SKU Unit Size Price Qty
DK-1488 1 kit

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 488 Antibody Kit

Description
Specifications
Kit Components
Documents
Related Products
Citations
Technical Information

Description

VectaFluor Excel Amplified DyLight® 488 Anti-Rabbit IgG offers a non-biotin amplification method for fluorescence applications. This system uses a ready-to-use (R.T.U.) Amplifier Antibody, followed by a ready-to-use (R.T.U.) VectaFluor DyLight dye-labeled detection system.

Features:

  • High sensitivity
  • Low background
  • VectaFluor DyLight 488 labeled anti-goat IgG antibody for detection
  • Excitation: 493 nm
  • Emission: 518 nm
  • Color: Green

 

Specifications

More Information
Unit Size 1 kit
Applications Immunofluorescence
Technology Amplified Fluorescence Systems
Conjugate DyLight 488
Color of Fluorescence Green
Target Species Rabbit
Format Ready-to-Use

Kit Components

Kit contents:

  • 15 ml 2.5% Normal Horse Serum for blocking
  • 15 ml Amplifier Antibody (Goat anti-rabbit IgG)
  • 15 ml VectaFluor DyLight labeled Horse Anti-Goat IgG

Documents

  • User Guide
  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 488 Antibody Kit

VECTASHIELD® Antifade Mounting Medium Catalog #: H-1000-10

VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 488 Antibody Kit

VECTASHIELD® Antifade Mounting Medium with DAPI Catalog #: H-1200-10

VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 488 Antibody Kit

VECTASHIELD® Antifade Mounting Medium with Propidium Iodide (PI) Catalog #: H-1300-10

VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 488 Antibody Kit

VECTASHIELD® HardSet™ Antifade Mounting Medium Catalog #: H-1400-10

VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 488 Antibody Kit

VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10

VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 488 Antibody Kit

VECTASHIELD® HardSet™ Antifade Mounting Medium with Phalloidin Catalog #: H-1600-10

Citations

VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 488 Antibody Kit Powered by Bioz See more details on Bioz

Technical Information

All the reagents in the VectaFluor Excel Kit are affinity purified and extensively cross-adsorbed to ensure high sensitivity and low background. In addition, the VectaFluor™ Reagents are conjugated to the DyLight dyes using technology that ensures maximum labeling without compromising antibody affinity or specificity. DyLight dyes offer several advantages such as brighter fluorescence, greater photostability, and pH independence.

All reagents are supplied in stabilized, R.T.U. solutions for maximum convenience.

Kit Contents:

  • 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking
  • 15 ml Amplifier Antibody (Goat anti-rabbit IgG; ready-to-use)
  • 15 ml VectaFluor™ DyLight® labeled Horse Anti-Goat IgG (ready-to-use)
 
  • Excitation maximum:  493 nm
  • Emission maximum:  518 nm
  • Color:  Green
VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 488 Antibody Kit

Home All Products Amplified Fluorescent Staining Systems VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 488 Antibody Kit

Vector Lab代理VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 594 Antibody Kit

发表于由

Vector Labs代理，欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 594 Antibody Kit

DK-1594

SKU Unit Size Price Qty
DK-1594 1 kit

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 594 Antibody Kit

Description
Specifications
Kit Components
Documents
Related Products
Citations
Technical Information

Description

VectaFluor Excel Amplified Fluorescent Staining System offers a non-biotin amplification method for fluorescence applications. This system uses a ready-to-use (R.T.U.) Amplifier Antibody, followed by a ready-to-use (R.T.U.) VectaFluor DyLight dye-labeled detection system.

Features:

  • High sensitivity
  • Low background
  • VectaFluor DyLight 594 labeled anti-goat IgG antibody for detection
  • Excitation: 592 nm
  • Emission: 617 nm
  • Color: Red

 

Specifications

More Information
Unit Size 1 kit
Applications Immunofluorescence
Technology Amplified Fluorescence Systems
Conjugate DyLight 594
Color of Fluorescence Red
Target Species Rabbit
Format Ready-to-Use

Kit Components

Kit contents:

  • 15 ml 2.5% Normal Horse Serum for blocking
  • 15 ml Amplifier Antibody (Goat anti-rabbit IgG)
  • 15 ml VectaFluor DyLight labeled Horse Anti-Goat IgG

Documents

  • User Guide
  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 594 Antibody Kit

VECTASHIELD® Antifade Mounting Medium Catalog #: H-1000-10

VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 594 Antibody Kit

VECTASHIELD® Antifade Mounting Medium with DAPI Catalog #: H-1200-10

VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 594 Antibody Kit

VECTASHIELD® HardSet™ Antifade Mounting Medium Catalog #: H-1400-10

VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 594 Antibody Kit

VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10

Citations

VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 594 Antibody Kit Powered by Bioz See more details on Bioz

Technical Information

All the reagents in the VectaFluor Excel Kit are affinity purified and extensively cross-adsorbed to ensure high sensitivity and low background. In addition, the VectaFluor Reagents are conjugated to the DyLight dyes using technology that ensures maximum labeling without compromising antibody affinity or specificity. DyLight dyes offer several advantages such as brighter fluorescence, greater photostability, and pH independence.

All reagents are supplied in stabilized, R.T.U. solutions for maximum convenience.

Kit Contents:

  • 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking
  • 15 ml Amplifier Antibody (Goat anti-rabbit IgG; ready-to-use)
  • 15 ml VectaFluor DyLightt labeled Horse Anti-Goat IgG (ready-to-use)

 

VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 594 Antibody Kit 

Home All Products Amplified Fluorescent Staining Systems VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 594 Antibody Kit

Vector Lab代理Mouse IgG, Control Antibody

发表于由

Vector Labs代理，欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

Mouse IgG, Control Antibody

I-2000-1

SKU Unit Size Price Qty
I-2000-1 1 mg

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Mouse IgG, Control Antibody

Description
Specifications
Documents
Product FAQs
Related Products
Citations
Technical Information

Description

This IgG preparation is intended for use as a control for primary antibodies made in mouse. This antibody has been purified from pooled serum of healthy adult animals and contains a spectrum of the IgG subclasses present in serum. The control antibody should be applied to the tissue section at the same concentration as the primary antibody to indicate whether staining is specific for the antigen or is nonspecific adsorption of primary antibody to tissue sites.

Specifications

More Information
Unit Size 1 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications
Recommended Storage 2-8 °C; Store frozen for long term storage
Recommended Usage Reconstitute by adding 0.5 ml water.The resulting solution will have the following composition:10 mM phosphate, 0.15 M NaCl, pH 7.8, 0.08% sodium azide, 20 mg/ml sucrose. ALLOW THE PROTEIN TO DISSOLVE WITHOUT VIGOROUS AGITATION.
Conjugate Unconjugated
Host Species Mouse

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

Product FAQs

Could you please describe what would be an appropriate negative control for IHC that can be used to help validate tissue staining results?

A commonly used negative control is omission of the primary antibody. While this control addresses whether the secondary antibody reagents are a source of staining, inadvertent binding of the primary antibody to the tissue can occur. Various tissue elements such as Fc receptors and charged molecules may bind the primary antibody non-specifically. Simply omitting the primary antibody as a negative control would miss potential false positive staining by this means. A few “publication worthy” negative controls for IHC are listed below: 1) Preabsorption of primary antibody with the immunogen used to generate the antibody can be employed. The working dilution of the primary antibody and an optimized concentration of the immunogen are incubated together for a period prior to application to the specimen. Lack of staining would indicate specificity of the primary antibody to the target antigen in solution. Positive staining using this method, however, may indicate lack of specificity of the primary antibody and/or the primary antibody is being bound by tissue elements. To rule out the latter, this control can be used in combination with suggestion no. 2 below. Note that adsorption controls are not always feasible or practical depending on the cost or source of the immunogen. 2) Use of an isotype control (e.g. “non-immune” mouse IgG), matched to that of the primary antibody and applied at the same protein concentration as the primary antibody, is probably the most widely used negative control. This control addresses whether tissue elements are inadvertently binding immunoglobulin from the same species as the primary antibody, in addition to non-specific binding from the secondary detection reagents. In most cases, use of a sub-class of isotype immunoglobulin (e.g. mouse IgG2a or IgG2b) is not required. Note that the use of pre-immune immunoglobulin, obtained prior to immunization, could also be used. However, it is very unusual for commercial vendors to offer pre-immune immunoglobulin. 3) Substitution of the primary antibody with an “irrelevant antibody” is also a suitable negative control. The term “irrelevant” refers to a primary antibody of the same isotype as the specific primary antibody (i.e. mouse IgG) and applied at the same concentration, that is known not to bind to a target in the tissue specimen. An example would be an anti-cytokeratin antibody on smooth muscle tissue. As with negative control no. 1 above, lack of staining indicates tissue elements are not binding this isotype of immunoglobulin. 4) In some cases, the target antigen can be removed from the tissue specimen as a sort of “knock-out” preparation. Once the target antigen has been removed, the complete assay is run to determine lack of staining. One method to remove the target antigen is using defined enzyme digestion. Examples include the use of a collagenase if the target antigen is collagen, or hyaluronidase if the target antigen is hyaluronic acid. Of course, there are limitations to this approach, however, variations of this “deletion” or “knock-out” approach would be valid negative controls.

Advice? I’m new to immunohistochemistry, do you have any recommendations for how best to get started?

Yes we do. Please visit our comprehensive Immunohistochemistry (IHC) page for assistance with each step of the IHC workflow. This resource contains a great deal of technical material including product recommendations and links to other technical documentation / videos / webinars.

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Mouse IgG, Control Antibody Powered by Bioz See more details on Bioz

Technical Information

 

Home All Products Control Antibodies Mouse IgG, Control Antibody