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Vector Methyl Green stains cell nuclei light green. It is an excellent alternative in multiple labeling when hematoxylin obscures the substrate colors.
Features:
Simple, two-step procedure
Suitable for use with a wide range of enzyme substrates
Suitable for use with non-aqueous mountants
Specifications
More Information
Unit Size
500 ml
Applications
Immunohistochemistry / Immunocytochemistry, In situ hybridization
Format
Ready-to-Use
Documents
User Guide
Counterstain/Substrate Compatibility Chart
Safety Data Sheet
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Product FAQs
I am using Methyl Green (H-3402) as a counterstain for my IHC, but the stain is a little weak. What suggestions do you have to increase the staining intensity?
Methyl Green counterstain does take a little bit of optimization in some applications. We would suggest heating a volume of the counterstain (~300 ml) to 60 °C and add to a Coplin jar or similar glass staining rack, and submerse the slides. This approach facilitates a better uptake of stain into the section compared with placing the slide on a heated surface and placing drops of stain onto the section. Following the incubation time (~3-5 min), remove slides and wash in tap water. Omit the acetone, acetic acid rinse step described in the instructions and move the slides directly into 95% ethanol for the dehydration, clearing and mounting process. This methodology will retain more stain in the nuclei and hence produce greater intensity.
Related Products
Hematoxylin QS Counterstain Catalog #: H-3404-100
VectaMount® Permanent Mounting Medium Catalog #: H-5000-60
Nuclear Fast Red Counterstain Catalog #: H-3403-500
Hematoxylin Counterstain Catalog #: H-3401-500
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Counterstain/Substrate Compatibility Table
This table is designed as a reference to determine the optimal counterstain/substrate combination for your application.
Home All Products Counterstains and Special Stains Methyl Green Counterstain
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Vector Hematoxylin QS (H-3404), a modification of Mayer’s hematoxylin developed especially for immunocytochemistry, provides crisp blue nuclear staining. This formula is ready-to-use without filtration, requires no separate bluing step, and stains in less than 45 seconds.
Features:
Provides excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates
Suitable for use with nonaqueous and aqueous mountants
Mercury-free
Specifications
More Information
Unit Size
100 ml
Applications
Immunohistochemistry / Immunocytochemistry, In situ hybridization
Format
Ready-to-Use
Documents
User Guide
Counterstain/Substrate Compatibility Chart
Safety Data Sheet
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Datasheet
Related Products
VectaMount® Permanent Mounting Medium Catalog #: H-5000-60
VectaMount® AQ Aqueous Mounting Medium Catalog #: H-5501-60
Hematoxylin Counterstain Catalog #: H-3401-500
Methyl Green Counterstain Catalog #: H-3402-500
Nuclear Fast Red Counterstain Catalog #: H-3403-500
Hematoxylin and Eosin Stain Kit Catalog #: H-3502
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Technical Information
Counterstain/Substrate Compatibility Table
This table is designed as a reference to determine the optimal counterstain/substrate combination for your application.
Home All Products Counterstains and Special Stains Hematoxylin QS Counterstain
Goat Anti-Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL, BSL), Unconjugated
AS-2104-1
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These highly purified specific antibodies provide excellent intermediate reagents for localizing lectin receptors in tissues and on cells or following lectin transport in neuronal tracing.
These antibodies to lectins are produced by immunizing goats with pure lectins. Following conventional purification steps, specific antibody is isolated by affinity chromatography on lectin-agarose columns. Each anti-lectin is supplied in solution.
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These highly purified specific antibodies provide excellent intermediate reagents for localizing lectin receptors in tissues and on cells or following lectin transport in neuronal tracing.
These antibodies to lectins are produced by immunizing rabbit with pure lectins. Following conventional purification steps, specific antibody is isolated by affinity chromatography on lectin-agarose columns. This anti-lectin is supplied in solution.
Goat Anti-Ricinus Communis Agglutinin (RCA) I & II, Unconjugated
AS-2084-1
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These highly purified specific antibodies provide excellent intermediate reagents for localizing lectin receptors in tissues and on cells or following lectin transport in neuronal tracing.
10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 20 mg/ml sucrose.
Recommended Usage
The recommended concentration range for use is 1 to 10 µg/ml.
Detection Target
Lectin
Conjugate
Unconjugated
Host Species
Goat
Format
Concentrate
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Safety Data Sheet
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Related Products
Ricinus Communis Agglutinin I (RCA I, RCA120), Unconjugated Catalog #: L-1080-10
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Technical Information
Each anti-lectin is supplied in solution. These antibodies to lectins are produced by immunizing goats with pure lectins. Following conventional purification steps, specific antibody is isolated by affinity chromatography on lectin-agarose columns.
Home All Products Antibodies to Lectins Goat Anti-Ricinus Communis Agglutinin (RCA) I & II, Unconjugated
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Con A recognizes α-linked mannose present as part of a “core oligosaccharide” in many serum and membrane glycoproteins.
Biotinylated Concanavalin A has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer.
Recommended Storage
2-8 °C; Store frozen for long term storage
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.01 mM MnCl2, 0.1 mM CaCl2, 0.08% sodium azide, 10 mM alpha methylmannoside.
Concentration
5 mg/ml
Conjugate
Biotinylated
Sugar Specificity
Mannose, Glucose
Documents
Lectins in Histochemistry, ELISA, and Western Blot Applications
Safety Data Sheet
Download CoA
Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is meet. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.
Related Products
α-methylmannoside Catalog #: S-9005
α-methylglucoside Catalog #: S-9006
Concanavalin A (Con A), Unconjugated Catalog #: L-1000-500
Concanavalin A (Con A), Fluorescein Catalog #: FL-1001-25
Concanavalin A (Con A), Rhodamine Catalog #: RL-1002-25
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At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits; below pH 5.6, Con A dissociates into active dimers of 52 kDa. Acetylation, succinylation, or other derivatizations can also produce stable forms with dimeric structures. (See succinylated Con A). Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds.
Con A requires calcium or manganese ions at each of its four saccharide binding sites. Although these divalent metal ions are bound tightly to the polypeptide structure, buffers which can bind calcium (such as phosphate) generally should be avoided in diluting Con A, since a gradual loss in activity may occur.
This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN® ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside
Home All Products Concanavalin A (Con A) Concanavalin A (Con A), Biotinylated
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The carbohydrate binding site recognizes (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0.
DSL also binds well to N-acetyllactosamine and oligomers containing repeating N-acetyllactosamine sequences. A branched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (β-1,6) and (β-1,2) was reported to be the most potent inhibitor of agglutination.
Biotinylated Datura stramonium lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer.
Recommended Storage
2-8 °C; Store frozen for long term storage
Solution
10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration
2 mg active conjugate/ml
Conjugate
Biotinylated
Sugar Specificity
[GlcNAc]1-3, N-Acetylglucosamine
Documents
Lectins in Histochemistry, ELISA, and Western Blot Applications
Safety Data Sheet
Download CoA
Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is meet. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.
DSL contains two chains of 40 kDa and 46 kDa joined by disulfide bonds. This lectin is free of a reported 32 kDa contaminant protein.
This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Inhibiting/Eluting Sugar: Chitin Hydrolysate
Home All Products Datura stramonium lectin (DSL) Datura Stramonium Lectin (DSL), Biotinylated
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Galanthus nivalis lectin, unlike most mannose-specific lectins, is not a metalloprotein and does not require Ca++ or Mn++ for binding.
Binding seems to be preferentially directed toward structures containing (α-1,3) mannose residues. Also in contrast to most mannose-binding lectins, GNL will not bind α-linked glucose. Reports indicate that this lectin binds rat and mouse IgM but not IgG. The only protein from human serum reported to bind to this lectin is α2-macroglobulin. GNL binds to many viral glycoproteins.
Biotinylated Galanthus nivalis lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer. A precipitate may form during storage.This does not have a significant adverse effect on the product.If a precipitate forms upon long-term storage, warm to 37 ºC and centrifuge before use.
Recommended Storage
2-8 °C; Store frozen for long term storage
Solution
10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration
2 mg/ml
Conjugate
Biotinylated
Sugar Specificity
Mannose
Documents
Lectins in Histochemistry, ELISA, and Western Blot Applications
Safety Data Sheet
Download CoA
Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is meet. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.
This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Inhibiting/Eluting Sugar: 100 mM - 200 mM α-methylmannoside
Home All Products Galanthus nivalis lectin (GNL) Galanthus Nivalis Lectin (GNL), Biotinylated
Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Biotinylated
B-1105-2
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GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed “A” and “B”, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The “A”-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin. Biotinylated GSL I has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin.
For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer.
Recommended Storage
2-8 °C; Store frozen for long term storage
Inhibiting and/or Eluting Sugar
Inhibiting/Eluting Sugar: Mixture of 200 mM galactose (S-9003)/200 mM N-acetylgalactosamine (S-9001).
Solution
10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration
2 mg active conjugate/ml
Conjugate
Biotinylated
Sugar Specificity
Galactose, N-Acetylgalactosamine
Documents
Lectins in Histochemistry, ELISA, and Western Blot Applications
Safety Data Sheet
Download CoA
Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is meet. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.
Related Products
Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins Catalog #: ES-2100-100
N-acetylgalactosamine Catalog #: S-9001
Galactose Catalog #: S-9003
Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Unconjugated Catalog #: L-1100-5
Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Rhodamine Catalog #: RL-1102-2
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This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Inhibiting/Eluting Sugar: mixture of 200 mM galactose/200 mM N-acetylgalactosamine
Home All Products Griffonia simplicifolia lectin I (GSL I) Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Biotinylated
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