Hampton 蛋白结晶试剂–Crystal Screen

Crystal Screen • Crystal Screen 2 • Crystal Screen HT

Description
The Crystal Screen and Crystal Screen 2 reagent kits are designed to provide a highly effective and rapid screening method for the crystallization of macromolecules. The screens are simple and practical for finding initial crystallization conditions. The initial crystallization conditions for more than 1,000 proteins, peptides, oligonucleotides, and small molecules have been determined using Crystal Screen.

A highly effective approach to overcome the exhaustive search for suitable crystallization conditions is the use of a sparse matrix method of trial conditions that is biased and selected from known crystallization conditions for macromolecules. The formulation utilized in Crystal Screen and Crystal Screen 2 evaluates 96 unique mixtures of pH, salts, polymers and organics, and their ability to promote crystal growth.

Crystal Screen contains 50 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by Jancarik and Kim in 1991.

Crystal Screen 2, an extension of Crystal Screen, contains 48 unique reagents, 10 ml each and is based on the formulation first described by Cudney et al in 1994.

Crystal Screen HT contains 1 ml each of reagents 1-48 from Crystal Screen and all 48 reagents from Crystal Screen 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

Crystals of a carbohydrate processing enzyme grown using Hampton Research Crystal Screen. Shirley Roberts, York Structural Biology Laboratory, York, United Kingdom.

Crystal of zRFP574 red fluorescent protein grown using Hampton Research Crystal Screen 2. Pletneva et al. Acta Cryst. D62, 527-532 (2006). Nadya, Russian Academy of Science, Moscow, Russia.

Hampton 24孔坐滴板 Cryschem Plate

Applications

 Sitting drop crystallization

  Heavy atom soaks

  Seeding

The Cryschem Plate is a 24 well sitting drop plate that is sealed with clear sealing tape. The polystyrene plate is supplied with a raised cover so the plate can also be sealed with vacuum grease and plain glass or plastic cover slides (22 mm diameter). The stackable, 24 well plates have distortion-free, flat bottom wells and a concave sitting drop post in the center of the well for concentric equilibration of the drop with the reagent. Each plate is individually sealed. The top of the plate and each of the 24 wells is a single, flat plane for smooth and easy seal application. Well spacing provides for a large seal area between wells to prevent cross contamination and evaporation.

上海金畔生物作为Hampton蛋白结晶产品的代理商，竭诚为您服务，欢迎新老客户咨询。

Cryschem Plate is large plate footprint with circular reagent reservoir. Cryschem plate approximate size: 15.1 cm x 10.6 cm x 2.2 cm. Approximate well size: 1.6 cm x 1.5 cm. Typical fill volume: 500 to 1,000 µl. Well capacity: 1.5 ml. Maximum drop volume on post: 40 µl. Recommended seal: HR3-511 Crystal Clear Sealing Tape (1.88 inch x 43.7 yard roll, with cutter) or HR4-511 Crystal Clear Sealing Tape (1.88 inch x 60 yard roll, without cutter).

Cryschem M Plate is microplate footprint with circular reagent reservoir. Cryschem M microplate approximate size: 128 mm x 86 mm x 14 mm. Approximate circular reservoir size: 15 mm wide x 9 mm deep. Typical reservoir volume: 500 µl. Well capacity: 1.1 ml. Maximum drop volume on post: 30 µl. Recommended seal: HR3-609 Crystal Clear Sealing Film (100 pack), HR4-521 ClearSeal Film (100 pack) or HR4-506 Crystal Clear Sealing Tape (3 inch x 55 yard roll, without cutter). HR4-508 Crystal Clear Sealing Tape (0.75 inch x 650 inch, with cutter) seals a single row at a time.

Cryschem S Plate is microplate footprint with square reagent reservoir. Cryschem S microplate approximate size: 128 mm x 86 mm x 14 mm. Approximate square reservoir size: 15 mm wide x 9 mm deep. Typical reservoir volume: 500 µl. Well capacity: 1.1 ml. Maximum drop volume on post: 30 µl. Recommended seal: HR3-609 Crystal Clear Sealing Film (100 pack), HR4-521 ClearSeal Film (100 pack) or HR4-506 Crystal Clear Sealing Tape (3 inch x 55 yard roll, without cutter). HR4-508 Crystal Clear Sealing Tape (0.75 inch x 650 inch, with cutter) seals a single row at a time.

Cryschem plate

Hampton 96孔坐滴蛋白结晶板–Intelli-Plate

在生命科学领域，上海金畔生物为业界提供了丰富的实验室和生物医药生产研发的产品。在这里要感谢广大客户多年来对上海金畔生物科技有限公司的支持和厚爱，我们将一如既往的为广大客户带来Hampton Intelli-Plate 96孔蛋白结晶板高品质的产品和服务，欢迎广大新老客户来电咨询。

Intelli-Plate 96 (Art Robbins Instruments)

Applications

  Sitting Drop Crystallization   用于蛋白坐滴结晶

Features  特征：

Description

Art Robbins Instruments has developed the ideal family of plates for crystallography applications: the Intelli-Plate. All versions of the Intelli-Plate have the SBS microplate footprint and standard well spacing and are ideal for manual or automated processing.

The Intelli-Plate 96-2 Low Profile (Art Robbins 102-0001-10 Hampton Research HR3-116 and HR3-117) is a low profile version of the Intelli-Plate 96-2 LVR (Low Volume Reservoir). Reservoir volume: 100 µl max. Drop well volume: 4 µl and 10 µl max. UV transmissible and low birefringence.

The CrystalMation Intelli-Plate 96-3 low-profile crystallization plate (Art Robbins 102-0001-13 Hampton Research HR3-118 and HR3-119) is a low profile version of the Intelli-Plate 96-3 LVR (Low Volume Reservoir). The plate is designed for sitting drop vapor diffusion crystallization experiments. It is constructed from optically clear, UV-transmissible, chemically resistant plastic with superior low birefringence. The low-profile construction allows for higher density in plate storage and imaging systems, maximizing your investment and valuable space. The plate is designed to be a substantial improvement in existing low-profile plates, allowing for superior sealing due to thicker well design and a flat sealing surface. It has been built to the SBS (Society for Biomolecular Screening) standard dimensions with 8 vertical wells along the left side of the plate (A-H) and 12 horizontal wells along the top of the plate (1-12). It is compatible with automated instrumentation including the CrystalMation line of instruments from Rigaku. The plate features three identical sized sample drop wells per reservoir. The wells are concave depressions along the left side (Y-axis) of the plate and are located on the ledge above the adjacent, flat bottom reagent reservoir. Each well features a round bottom for easy crystal harvesting and can hold up to 1 µl of sample. The multiple well design allows for combinatorial experiments, complex co-crystallization experiments for ligand screening, or for drop concentration variation. The reagent reservoir is typically filled with 50 to 70 µl of reagent. Reservoir volume: 100 µl max. Drop well volume: 1 µl max. UV transmissible and low birefringence.

The Intelli-Plate 96-2 LVR (Art Robbins 102-0001-00 Hampton Research HR3-143 and HR3-145) is a Low Volume Reservoir version of the original Intelli-Plate. Reservoir volume: 100 µl max. Drop well volume: 1 µl max. UV transmissible and low birefringence.

The Intelli-Plate 96-3 LVR (Art Robbins 102-0001-03 Hampton Research HR3-183 and HR3-185) is a Low Volume Reservoir, 3 drop well version of the Intelli-Plate. Reservoir volume: 100 µl max. Typical reservoir volume 50 µl. Drop well volume: 1 µl max. UV transmissible and low birefringence.

The Intelli-Plate 96-2 Original crystallization plate (Art Robbins 102-0011-00 Hampton Research HR3-297 and HR3-299) is an optically clear plate for sitting drop vapor diffusion crystallization. All wells have standard 9 mm spacing to conform to SBS standards with 8 vertical wells along the left side of the plate (A-H) and 12 horizontal wells along the top of the plate (1-12). This plate features two locations for sample per reservoir. The sample drop locations are located along the left side of the reagent reservoir, along the Y-axis of the plate. The sample wells are concave depressions on the ledge above the adjacent, flat bottom reagent reservoir. One sample well is located above the second sample well. The top sample well can hold 10 µl or less of sample. The lower sample well can hold 4 µl or less of sample. The reagent reservoir is typically filled with 100 µl of reagent and is capable of holding up to 300 µl of reagent. The sidewalls separating adjacent wells or reservoirs are 0.9 and 1.0 mm thick in order to offer a larger area for sealing the plate and a separation of the reservoirs. This plate is rigid, with virtually no torsional flex and is designed for either manual or automated pipetting. UV transmissible and low birefringence.

The Intelli-Plate Flat Shelf (Art Robbins 102-0001-01 Hampton Research HR8-171 and HR8-172) crystallization plate is a flat drop shelf version of the original Intelli-Plate. Reservoir volume: 300 µl max. No drop well. UV transmissible and low birefringence.

The Intelli-Plate 96-2 Shallow Well (Art Robbins 102-0001-20 Hampton Research HR3-163 and HR3-164) has two identically sized 2 µL reaction wells and a 140 µL screen reservoir. Each of the two identical well features a round bottom shallow well design for easy crystal harvesting and is arranged in an 8 x 12 array. This plate has low birefringence and is UV compatible. This plate is also available in a low profile height (Art Robbins 102-0001-21).

The plates can be sealed using Crystal Clear Sealing Film (HR3-609), 3 inch wide Crystal Clear Sealing Tape (HR4-506) or ClearSeal Film™ (HR4-521).

The height of Intelli-Plate is 0.560 inches. The height of Intelli-Plate Low Profile is 0.315 inches (8.001 mm).

Intelli-Plate 96-3

Hampton蛋白悬滴结晶板 HDP Hanging Drop Crystallization Plate

美国Hampton Research公司位于加利福尼亚州，是一家专门从事蛋白质晶体研究的生产商。该公司向全球科研人员提供全面的晶体研究试剂和相关实验耗材，同时提供晶体设计和合成方面的定制服务。Hampton Research公司以其先进的生物大分子结晶技术和广泛实用的产品线，不仅为全世界晶体研究人员的工作带来了便捷，而且也受益于科研人员的回馈不断发展自己，目前已成为晶体研究领域最受信赖的品牌之一。

Hampton research主要产品：

Crystallization Screens 用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液

Optimize Reagents 结晶级别盐类，聚合物，有机溶液或缓冲液

Crystallization Plates, Hardware & Accessories 结晶板（悬滴法和坐滴法，微量透析，微配液，自由界面扩散，凝胶以及无容器悬浮法）及其他配件

Cryocrystallography 低温晶体学耗材如 Crystalcap system,cryoloops

Goniometer Heads&Supplies 测角器头和配件

Protein Crystallization Standards 结晶级别标准蛋白

Custom Shop Crystallization Reagents 定制试剂盒和结晶溶液内的单一组分

StockOptions Kits 内含盐类，缓冲液和冷冻液的简便型试剂盒

Tools，Seeding&Resin 结晶用探针，结晶笔，seeding法结晶工具

Capillary Mounts & Supplies 毛细管,玻璃纤维, 蜡,黏合剂和密封剂

Hampton蛋白悬滴结晶板 HDP Hanging Drop Crystallization Plate

HDP Hanging Drop Plate

Single Well with Tool

Hanging Drop Wells

Applications—Hanging drop crystallization

Features

  Made from optically superior polymer (UVP)

  Allen key novel crystal plug closure system

  Wells are micro-numbered

Description  描述：
The Swissci Hanging Drop Crystallization Plate is a breakthrough for protein crystallization in a 96-well format. For the first time individual wells can be identified and removed without any disturbance to the growing crystals inside the plate.Description

The plate has been developed by Swissci AG together with leading Crystallographers. It is the result of many years of experience in successful robotic high-throughput crystallization.

The Hanging Drop Crystallization Plate offers unique properties that make it ideal for both nanoliter crystallization screening and microliter optimization alike. Made from optically superior polymer (UVP) and with a new design of the wells, the plate allows easy crystal viewing and retrieval. The system is fully covered by design and patent protection.

The Advantages of the Hanging Drop Crystallization Plate

Easy Crystal Retrieval
Raised wide wells make the crystal mounting especially easy. Allen key system allows for smooth removal and subsequent microscopic investigation / X-ray with the novel crystal plug closure all included in the system.

Easy Viewing
The wells are wide conical and have a lens effect for perfect illumination. The micro-numbering ensures you will never loose the location under the microscope.

The optically superior polymer is UV transmissible and can be used to differentiate between salt and protein crystals.

Integral Sealing
Wide partition walls between each well provides a high surface area for very good sealing with the advanced quality sealing tape. The sealing tape is integrated into the kit so there is no need to purchase additional parts prior to starting a procedure.

Wide Range of Volumes
Typical volumes are 50 – 200 µl of reservoir and 10 nanoliter – 5 microliter drop size.

SBS Standard
The complete plate is designed to the 96-well SBS standard for all common holders.

Hampton蛋白悬滴结晶板 HDP Hanging Drop Crystallization Plate 相关产品：

Hampton蛋白质结晶捞针（结晶环）18 mm Mounted CryoLoop™

Applications

Cryocrystallography

Features

		Adjustable loop orientation
		Synthetic CryoLoop on adjustable stainless steel MicroTube
		MicroTube specially engineered with EasySnap notches
		20 micron diameter nylon CryoLoop

Description
Similar to the Mounted CryoLoop™ in design and function, the Adjustable Mounted CryoLoop allows one to manipulate and orient the CryoLoop to multiple, unique positions previously unattainable with typical cryo setups. The Adjustable Mounted CryoLoop is a 20 micron diameter synthetic loop mounted inside a malleable stainless steel sleeve, which in turn, is mounted inside the EasySnap MicroTube™.

Using a specially designed L-shaped tool (HR5-902) with a positioning notch, one can manipulate the angle and orientation of the CryoLoop by bending and adjusting the angle of the malleable stainless steel insert which holds the CryoLoop. The Adjustable Mounted CryoLoop may be positioned several times. Note: Repeated manipulation will fatigue the sleeve which can lead to failure of the sleeve. The Adjustable Mounted CryoLoop is designed to work with the CrystalCap System components.

The MicroTube is 24 mm in length and is specially engineered with EasySnap™ notches at the 10, 12, 14, 18, and 21 mm measures. To obtain the desired length of MicroTube, simply snap the MicroTube at the desired length and stake it to the CrystalCap™ Magnetic or CrystalCap™ Copper Magnetic. These nylon loops show minimal diffraction, are thin for fast freezing, strong, and aerodynamic.

Most data collection geometries prefer or require a 22 mm length between the base of the cap and the beam. For a 22 mm overall length using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap SPINE HT, snap the MicroTube at the second notch from the bottom, creating an 18 mm MicrozTube lenghth and secure to the CrystalCap using epoxy or similar adhesive. For a 22 mm over length using the CrystalCap Copper, CrystalCap Copper HT, CrystalCap ALS Copper HT snap the MicroTube at the fifth notch from the bottom (notch closest to the CryoLoop) and secure to the CrystalCap using epoxy or similar adhesive.

The HR5-902 Tool for Adjustable Mounted CryoLoop is not included with the Adjustable Mounted CryoLoop and must be purchased as a separated item.

18 mm Mounted CryoLoop

18 mm Mounted CryoLoop properly installed in a CrystalCap Magnetic

Crystal in CryoLoop

Hampton research HR2-110 Crystal Screen

Hampton research主要产品：
Crystallization Screens 用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液
Optimize Reagents 结晶级别盐类，聚合物，有机溶液或缓冲液
Crystallization Plates, Hardware & Accessories 结晶板（悬滴法和坐滴法，微量透析，微配液，自由界面扩散，凝胶以及无容器悬浮法）及其他配件
Cryocrystallography 低温晶体学耗材如Crystalcap system,cryoloops
Goniometer Heads&Supplies 测角器头和配件
Protein Crystallization Standards 结晶级别标准蛋白
Custom Shop Crystallization Reagents 定制试剂盒和结晶溶液内的单一组分
StockOptions Kits 内含盐类，缓冲液和冷冻液的简便型试剂盒
Tools，Seeding&Resin 结晶用探针，结晶笔，seeding法结晶工具
Capillary Mounts & Supplies 毛细管,玻璃纤维, 蜡,黏合剂和密封剂

Hampton research HR2-110 Crystal Screen

 Primary screen for proteins, soluble peptides, nucleic acids, & water soluble small molecules

 Sparse matrix additive screen

Description
The Crystal Screen and Crystal Screen 2 reagent kits are designed to provide a highly effective and rapid screening method for the crystallization of macromolecules. The screens are simple and practical for finding initial crystallization conditions. The initial crystallization conditions for more than 1,000 proteins, peptides, oligonucleotides, and small molecules have been determined using Crystal Screen.

A highly effective approach to overcome the exhaustive search for suitable crystallization conditions is the use of a sparse matrix method of trial conditions that is biased and selected from known crystallization conditions for macromolecules. The formulation utilized in Crystal Screen and Crystal Screen 2 evaluates 96 unique mixtures of pH, salts, polymers and organics, and their ability to promote crystal growth.

Crystal Screen contains 50 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by Jancarik and Kim in 1991.

Crystal Screen 2, an extension of Crystal Screen, contains 48 unique reagents, 10 ml each and is based on the formulation first described by Cudney et al in 1994.

Crystal Screen HT contains 1 ml each of reagents 1-48 from Crystal Screen and all 48 reagents from Crystal Screen 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.