标签归档：lumiprobe一级代理

Coumarin 343 azide荧光染料

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Coumarin 343 azide荧光染料

Lumiprobe Corporation是美国一家高品质生物技术公司，专业提供分子生物学研究用的活性荧光染料。从2006年开始，公司生产并销售生命科学研究和诊断学应用的优质化学药品。产品主要有：活性染料(Reactive Dye)和SYBR Green I 染料，用于寡核苷酸合成的亚磷酰胺，点击化学用染料和其它试剂。 产品主要应用：点击化学(Click Chemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2D DIGE)和实时荧光定量PCR(Realtime PCR)。其中，氨基类染料是包含自由氨基的活性染料，染料可与活化羧酸衍生物和其他亲电子的试剂结合。比如：氨基与EDC-活化的羧基结合。

 

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商，上海金畔生物Lumiprobe官网：http://lumiprobe.jinpanbio.com/。可提供Lumiprobe的完整产品线，所有规格产品保证货期5-7工作日到货，满足您的各种科研实验需求。Lumiprobe产品包括：羧酸类染料、羰基类染料，标记分子氨基活性染料，标记分子巯基活性染料，动物活体成像用染料，蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规（市场上部分是分装，质量不能保证），质量保证，价格极具竞争力,而且我们的货期仅需要5-7工作日，不需要像其他家攒单导致货期3-4周。

Coumarin 343 azide荧光染料

This product will be discontinued soon in favor of Coumarin 343 X azide. We may still have some material in stock (please see availability above).

This greenish-blue emitting fluorophore can be used as a FRET donor for FAM. Azide derivative is suitable for Click Chemistry.Coumarin 343 azide structure

该蓝绿色光荧光染料可以用于FAM的FRET供体。叠氮类衍生物可以用于点击化学。

订购货号 产品名称及规格
A1430 Coumarin 343 azide, 1 mg
B1430 Coumarin 343 azide, 5 mg
C1430 Coumarin 343 azide, 10 mg
D1430 Coumarin 343 azide, 25 mg
E1430 Coumarin 343 azide, 50 mg

General properties

Appearance: yellow powder
Molecular weight: 441.48
Molecular formula: C22H27N5O5
Solubility: Good in organic solvents (DCM, chloroform, DMF, DMSO, MeCN, alcohols), poor in water
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download

Spectral properties

Excitation maximum, nm: 437
Extinction coefficient at excitation maximum, Lmol-1cm-1: 44000
Emission maximum, nm: 477
Fluorescence quantum yield: 0.63

Cy7-N-羟基琥珀酰胺酯|CAS 477908-53-5|Sulfo-cyanine7 NHS ester

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Cy7-N-羟基琥珀酰胺酯|CAS 477908-53-5|Sulfo-cyanine7 NHS ester

产品简介

胺类活性染料，近红外荧光染料，Cy7?的改进的类似物。近红外荧光素可以被用来利用生物组织的近红外窗口。在这个光谱区域，高度增强的组织透明度使得活体成像成为可能。该染料可用于制备CY7标记的生物分子来进行后续试验，如活体研究及药物开发相关实验。Cy7的特殊结构加固了中心聚亚甲基（polymethyne）链的稳定性。这种增强的分子结构会使其量子产率较其母结构提供20%，因此它的荧光强度会更大。

该试剂在标记时需要共溶剂有机溶剂。水溶Cy7 NHS ester可以用于蛋白NIR标记。

Amine reactive Cyanine7, near infrared fluorescent dye, an improved analog of Cy7®.

NIR fluorophores can be used to take advantage of near infrared window of biological tissues – increased transparency of tissues in this spectral region allows to carry out in vivo imaging.

This reagent can be utilized to produce Cyanine7-labeled biomolecules for subsequent use in various in vivo research, and drug design related experiments.

The structure of Cyanine7 features rigidized design of central polymethyne chain. This molecular reinforcement allows to increase quantum yield by 20% compared with parent structure, increasing fluorescence brightness.

This reagent requires organic co-solvent for the labeling (please see Recommended Protocols section below). Water-soluble Cyanine7 NHS ester is also available, and recommended for protein NIR labeling.

一般特性

 外观: green powder
分子量: 682.29
分子式: C41H48ClN3O4
溶解性: soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility   in water
质量控制: NMR 1H (95%) and 13C, TLC, functional testing
储存条件: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for   up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

光谱特性

激发波长, nm: 750
在激发波长下的消光系数, Lmol-1cm-1: 199000
发生波长, nm: 773
荧光量子产率: 0.3

Lumiprobe ROX NHS ester, pure 6- isomer

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Lumiprobe ROX NHS ester, pure 6- isomer

ROX(rhodamine X aka R101)是一种高荧光强度的、耐光的罗丹明染料，在多领域有广泛的应用。ROX标记的寡核苷酸探针常用于qPCR和有ROX通道的qPCR仪器。
该活性染料用于标记多肽、蛋白、氨基寡核苷酸的氨基基团。Pure single isomer.
ROX NHS ester可以替代Alexa Fluor 568。

ROX (rhodamine X aka R101) is a highly fluorescent, and photostable rhodamine dye for various applications. ROX labeled oligonucleotide probes are often used in qPCR, and qPCR instruments have ROX channel.

This is reactive dye for the labeling of amino-groups in peptides, proteins, and amino-oligonucleotides. Pure single isomer.

This dye can replace Alexa Fluor 568, Texas Red.

Absorption and emission spectra of ROX dyeROX NHS ester, pure 6- isomer

订购货号 产品名称及规格
12220 ROX NHS ester, pure 6- isomer, 1 mg
22220 ROX NHS ester, pure 6- isomer, 5 mg
42220 ROX NHS ester, pure 6- isomer, 25 mg
52220 ROX NHS ester, pure 6- isomer, 50 mg
62220 ROX NHS ester, pure 6- isomer, 100 mg

General properties

Appearance: dark crimson powder
Mass spec M+ increment: 516.2
Molecular weight: 631.67
Molecular formula: C37H33N3O7
CAS number: 117491-83-5
Solubility: good in polar organic solvents (DMF, DMSO), low in water
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 570
Extinction coefficient at excitation maximum, Lmol-1cm-1: 82000
Emission maximum, nm: 591
Fluorescence quantum yield: 1.0

Cy5.5 NHS ester 氨基反应性荧光染料,不溶于水

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Cy5.5 NHS ester 氨基反应性荧光染料,不溶于水

Cy5.5 NHS是近红外胺类活性染料。CY5.5 NHS ester是用于标记多肽、蛋白和寡核苷酸的氨基基团的活性染料。CY5.5属于是近红外荧光染料，当背景荧光有干扰时适宜用它来做标记以进行荧光分析。它也可以用于活体成像实验。该染料可以替代Alexa Fluor 680和DyLight 680。

Cyanine5.5 NHS ester是用于标记含有伯胺的多肽，蛋白和寡核苷酸的反应性染料。 Cy5.5-NHS ester是一种近红外荧光染料，能够溶解在DMF和DMSO中，最大激发光和最大发射光分别在675 nm和693 nm左右[1]。该试剂不仅用于标记含有伯基的分子，而且还用于标记质粒DNA [2]。在生物分子标记中，该标记试剂的水溶性较低，先使用有机溶剂溶解该分子对于高效标记是必需的。首先，Cyanine染料应该溶解在有机溶剂中，然后加入包含生物分子的合适缓冲水溶液中。

通过皮下移植肿瘤小鼠的光学成像，在0.5nmol Cy5.5-NHS注射后30min至24h，皮下U87MG肿瘤可与周围背景组织明显区分开来。 Cy5.5-NHS的肿瘤吸收在注射后30分钟达到最大值，随着时间的推移缓慢洗出[3]。

Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester.

Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.

This reagent can replace NHS esters of Cy5.5®, Alexa Fluor 680, and DyLight 680.

Cy5.5 NHS ester structureCyanine5.5 NHS ester

General properties

Appearance: dark blue powder
Molecular weight: 716.31
Molecular formula: C44H46ClN3O4
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 673
Extinction coefficient at excitation maximum, Lmol-1cm-1: 209000
Emission maximum, nm: 707
Fluorescence quantum yield: 0.2

氨基分子的NHS酯标记

NHS (N-HydroxySuccinimide) esters and other activated esters (sulfo-NHS, sulfotetrafluorophenyl – STP) are reactive compounds suitable for the modification of amino groups. NHS is most common type of activated esters.

Usual modifications are fluorescent labels, fluorescence quenchers, and other reporter groups. Alkyne and azido group can be attached using activated esters to adapt biomolecules to Click Chemistry.

Since amino groups are nearly always contained in proteins and peptides, modification of these biopolymers is especially common. Other examples are amino-oligonucleotides, amino-modified DNA, and amino-containing sugars.

The reaction of NHS esters with amines is strongly pH-dependent: at low pH, the amino group is protonated, and no modification takes place. At higher-than-optimal pH, hydrolysis of NHS ester is quick, and modification yield diminishes. Optimal pH value for modification is 8.3-8.5.

Water is most common solvent for the labeling. If NHS ester is poorly soluble, it can be added as a solution in DMSO or DMF to a solution of protein in water, adjusted to pH 8.3-8.5. Note that DMF must not contain amines (and thus should have no odor).

We recommend using the following general protocol for the labeling of biomolecules with NHS esters produced by Lumiprobe.

  1. Calculate required amount of NHS ester:NHS_ester_weight [mg] = 8 × amino_compound_weight [mg] × NHS_ester_molar_weight [Da] / amino_compound_molar_weight [Da].

    8 is molar excess of NHS ester. It is experimental value for mono-labeling, suitable for many common proteins and peptides. However, in some cases using less or more NHS ester is required. It depends on protein structure, reagent, and solubility. Molar weight of Lumiprobe products can be found on corresponding product pages.

    For example, to label 3 mg of insulin (molar weight 69300 Dalton) with Cy5 NHS ester (molar weight 592 Dalton), and obtain maximum yield of mono-labeled product, one should use
    10 × 3 mg × 592 Da / 69300 Da = 0.26 mg
    of Cy5 dye NHS ester.

  2. Determine volume of reaction mixture. The labeling can be performed on any scale from nanomols to dozens of grams. When the scale is low, use minimal volume (10-20 uL). Higher concentrations (1-10 mg of amino-biomolecule per mL of mixture) are optimal.
  3. Dissolve NHS ester in 1/10 reaction volume of DMF or DMSO. Amine-free DMF is preferred solvent. After the reaction, NHS ester can be stored in solution for 1-2 months at -20ºC.
  4. Dissolve biomolecule in 9/10 reaction volume of buffer with pH 8.3-8.5.0.1 M Sodium bicarbonate solution has appropriate pH. Other alternatives are 0.1 M Tris buffer (although Tris has amino group, it is hindered and does not react with NHS esters), or 0.1 M phosphate buffer. Note pH is the most important thing.

    When doing large-scale labeling (hundreds of milligrams of NHS ester), note that the mixture tends to acidify with time because of hydrolysis of NHS ester. Monitor pH, or use more concentrated buffer then.

  5. Add NHS ester solution to the solution of biomolecule, and vortex well. Keep on ice overnight, or at room temperature during at least 4 hours.
  6. Purify the conjugate using appropriate method: gel-filtration for macromolecules is most universal. Precipitation and chromatography is another alternative. Organic impurities (such as N-hydroxysuccinimide, NHS ester, acid produced by hydrolysis) are almost always easily separated. For proteins and nucleic acids, ethanol or acetone precipitation can be used.

Lumiprobe Cy3.5 NHS ester 氨基反应性荧光染料,不溶于水

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Lumiprobe Cy3.5 NHS ester 氨基反应性荧光染料,不溶于水

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商，上海金畔生物Lumiprobe官网：http://lumiprobe.jinpanbio.com/。可提供Lumiprobe的完整产品线，所有规格产品保证货期5-7工作日到货，满足您的各种科研实验需求。Lumiprobe产品包括：羧酸类染料、羰基类染料，标记分子氨基活性染料，标记分子巯基活性染料，动物活体成像用染料，蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规（市场上部分是分装，质量不能保证），质量保证，价格极具竞争力,而且我们的货期仅需要5-7工作日，不需要像其他家攒单导致货期3-4周。

服务热线:18301939375   QQ：3258089810    3259632176

Cyanine3.5 NHS ester (an analog of Cy3.5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.

Cyanine3.5 NHS ester can replace NHS esters of Cy3.5®, Alexa Fluor 594, DyLight 594.

订购货号 产品名称及规格
12020 Cyanine3.5 NHS ester, 1 mg
22020 Cyanine3.5 NHS ester, 5 mg
42020 Cyanine3.5 NHS ester, 25 mg
52020 Cyanine3.5 NHS ester, 50 mg
62020 Cyanine3.5 NHS ester, 100 mg

General properties

Appearance: dark purple powder
Molecular weight: 690.27
Molecular formula: C42H44ClN3O4
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H and HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

 

Excitation maximum, nm: 591
Extinction coefficient at excitation maximum, Lmol-1cm-1: 116000
Emission maximum, nm: 604
Fluorescence quantum yield: 0.35

 

Product citations

 

  • Wang, C.; Niederstrasser, H.; Douglas, P.M.; Lin, R.; Jaramillo, J.; Li, Y.; Olswald, N.W.; Zhou, A.; McMillan, E.A.; Mendiratta, S.; Wang, Z.; Zhao, T.; Lin, Z.; Luo, M.; Huang, G.; Brekken, R.A.; Posner, B.A.; MacMillan, J.B.; Gao, J.; White, M.A. Small-molecule TFEB pathway agonists that ameliorate metabolic syndrome in mice and extend C. elegans lifespan. Nature Communications, 2017, 8, 2270. doi: 10.1038/s41467-017-02332-3
  • Massey, M.; Kim, H.; Conroy, E.M.; Algar, W.R. Expanded Quantum Dot-Based Concentric Förster Resonance Energy Transfer: Adding and Characterizing Energy-Transfer Pathways for Triply Multiplexed Biosensing. The Journal of Physical Chemistry C, 2017, 121(24), 13345–13356. doi: 10.1021/acs.jpcc.7b02739
  • Wang, C.; Wang, Y.; Li, Y.; Bodemann, B.; Zhao, T.; Ma, X.; Huang, G.; Hu, Z.; DeBerardinis, R.J.; White, M.A.; Gao, J. A nanobuffer reporter library for fine-scale imaging and perturbation of endocytic organelles. Nature Communications, 2015, 6, 8524. doi:10.1038/ncomms9524
  • Fu, B.; Flynn, J.D.; Isaacoff, B.P.; Rowland, D.J.; Biteen, J.S. Super-Resolving the Distance-Dependent Plasmon-Enhanced Fluorescence of Single Dye and Fluorescent Protein Molecules. The Journal of Physical Chemistry C, 2015, 119(33), 19350–19358. doi: 10.1021/acs.jpcc.5b05154

 

Lumiprobe Sulfo-Cyanine7 NHS ester

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Lumiprobe Sulfo-Cyanine7 NHS ester

Sulfo-Cyanine7 NHS ester是水溶近红外染料sulfo-Cyanine7，是胺类活性琥珀酸亚胺酯。

Sulfo-Cyanine7是Cy7®荧光团的的改良衍生物，量子产率提高20%，并具有更好的耐光性。该染料尤其适用于NIR成像应用。

近红外荧光染料利用了生物组织在特定的波长范围里的透明度。这种方法是无损伤性的，并允许在活体内对多种已标记的生物分子的分布进行跟踪。

Sulfo-Cyanine7 NHS ester可以很轻松的标记生物分子，如：蛋白质。标记的分子可以用于后续多种研究及药物开发相关的实验。

这种染料有很高的水溶解度，尤其适用于痕量蛋白质和易变性蛋白质的标记。需有机溶剂才能溶解的非磺化的Cyanine7 NHS ester。

订购货号 产品名称及规格 价格（￥）
15320 Sulfo-Cyanine7 NHS ester, 1 mg 2080.00
25320 Sulfo-Cyanine7 NHS ester, 5 mg 5330.00
45320 Sulfo-Cyanine7 NHS ester, 25 mg 11050.00
55320 Sulfo-Cyanine7 NHS ester, 50 mg 19500.00
65320 Sulfo-Cyanine7 NHS ester, 100 mg 31850.00

General properties

Appearance: dark green powder
Molecular weight: 827.94
Molecular formula: C41H46N3NaO10S2
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

 

Excitation maximum, nm: 740
Extinction coefficient at excitation maximum, Lmol-1cm-1: 240600
Emission maximum, nm: 773
CF260: 0.04
CF280: 0.04

Product citations

  • Ayala-Orozco, C.; Liu, J.G.; Knight, M.W.; Wang, Y.; Day, J.K.; Nordlander, P.; Halas, N.J. Fluorescence Enhancement of Molecules Inside a Gold Nanomatryoshka. Nano Letters, 2014, 14(5), 2926-2933. doi: 10.1021/nl501027j
  • Brand, C.; Abdel-Atti, D.; Zhang, Y.; Carlin, S.; Clardy, S.M.; Keliher, E.J.; Weber, W.A.; Lewis, J.S.; Reiner, T. In Vivo Imaging of GLP-1R with a Targeted Bimodal PET/Fluorescence Imaging Agent. Bioconjugate Chemistry, 2014, 25(7), 1323-1330. doi:10.1021/bc500178d
  • Graen, T.M.D.; Hoefling, M.; Grubmüller, H. AMBER-DYES: Characterization of Charge Fluctuations and Force Field Parameterization of Fluorescent Dyes for Molecular Dynamics Simulations. Journal of Chemical Theory and Computation, 2014, 10(12), 5505-5512. doi: 10.1021/ct500869p
  • Li, L.-L.; Xu, J.-H.; Qi, G.-B.; Zhao, X.; Yu, F.; Wang, H. Core-Shell Supramolecular Gelatin Nanoparticles for Adaptive and “On-Demand” Antibiotic Delivery. ACS Nano, 2014, 8(5), 4975-4983. doi: 10.1021/nn501040h
  • Viehweger, K.; Barbaro, L.; García, K.P.; Joshi, T.; Geipel, G.; Steinbach, J.; Stephan, H.; Spiccia, L.; Graham, B. EGF Receptor-Targeting Peptide Conjugate Incorporating a Near-IR Fluorescent Dye and a Novel 1,4,7-Triazacyclononane-Based 64Cu(II) Chelator Assembled via Click Chemistry. Bioconjugate Chemistry, 2014, 25(5), 1011-1022. doi: 10.1021/bc5001388
  • Zhou, Z.; Wu, X.; Kresak, A.; Griswold, M.; Lu, Z.-R. Peptide targeted tripod macrocyclic Gd(III) chelates for cancer molecular MRI.Biomaterials, 2013, 34(31), 7683-7693. doi: 10.1016/j.biomaterials.2013.06.057
  • Zou, C.; Loka, R.S.; Zhang, Y.; Cairo, C.W. Glycoform Remodeling Generates a Synthetic T Cell Phenotype. Bioconjugate Chemistry, 2013, 24(6), 907-914. doi: 10.1021/bc300599w
  1. Nakamura, T.; Sugihara, F.; Matsushita, H.; Yoshioka, Y.; Mizukami, S.; Kikuchi, K. Mesoporous silica nanoparticles for 19F magnetic resonance imaging, fluorescence imaging, and drug delivery. Chemical Science, 2015. doi: 10.1039/c4sc03549f
  2. Aldeek, F.; Hawkins, D.; Palomo, V.; Safi, M.; Palui, G.; Dawson, P.E.; Alabugin, I.V.; Mattoussi, H. UV and Sunlight Driven Photoligation of Quantum Dots: Understanding the Photochemical Transformation of the Ligands. Journal of the American Chemical Society, 2015. doi: 10.1021/ja512802x
  3. Hsueh, P.-Y.; Edman, M.C.; Sun, G.; Shi, P.; Xu, S.; Lin, Y.-a.; Cui, H.; Hamm-Alvarez, S.F.; MacKay, J.A. Tear-mediated delivery of nanoparticles through transcytosis of the lacrimal gland. Journal of Controlled Release, 2015. doi: 10.1016/j.jconrel.2014.12.017
  4. Wen, A.M.; Infusino, M.; De Luca, A.; Kernan, D.L.; Czapar, A.E.; Strangi, G.; Steinmetz, N.F. Interface of Physics and Biology: Engineering Virus-Based Nanoparticles for Biophotonics. Bioconjugate Chemistry, 2015, 26(1), 51–62. doi: 10.1021/bc500524f

Cy3/5/7 NHS ester和带NH2多肽和抗体连接实验方法及步骤

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Cy3/5/7 NHS ester和带NH2多肽和抗体连接实验方法及步骤

Cy3 NHS ester, Cyanine5 NHS ester和Cyanine7 NHS ester可以标记含有amine的多肽，抗体，蛋白，DNA和寡核苷酸及其他小分子。在反应条件下NHS可以和NH2发生反应，从而连接到一起。

（一）：Cyanine5 NHS ester和多克隆抗体的连接实验方法和步骤

【准备材料】：

Cy3 NHS ester purchased from xi an ruixibio

anti-GST多克隆抗体purchased from Abcam

【实验步骤】：

1.      在1 L 0.15 M NaCl 溶液中透析anti-GST抗体(浓度为 0.5 mL at3 mg/mL)，常温透析4小时。

2.      在4°C用新鲜的1 L 0.15 M NaCl 溶液再透析过夜。

3.      第二天用1 L 0.1 M NaHCO3 (pH 8.3)透析4小时。

4.      用0.22 μm注射器过滤头过滤抗体溶液。

5.      用0.1 M NaHCO3 稀释少量的抗体，在280nm处测其紫外吸收值计算标记抗体的总量 (IgG antibody摩尔吸光系数170 000 M-1 cm-1 at 280 nm).

6.      用DMSO配置Cy3 NHS (MW 616.19)溶液浓度为10 mg/mL;计算所需体积以得到想要的Cy3NHS 和抗体的比值(例如20:1)，然后慢慢将其加入到抗体溶液中，同时在暗处常温缓慢搅拌45分钟。

7.      用1 L 0.15 M NaCl 溶液常温避光透析4小时除去未标记上的Cy5。

8.      在4°C用新鲜的1 L 0.15 M NaCl 溶液再避光透析过夜。

9.      用1 L of 0.01 M PBS/ 0.01% 叠氮化钠溶液常温避光透析4小时, 在4°C常温避光再次透析过夜。

10.  用0.22 μm注射器过滤头过滤抗体溶液。

11.  用0.01 M PBS/0.01 % 叠氮化钠整数倍稀释标记抗体溶液，测量280nm(蛋白)和552nm(Cy)处的紫外可见吸光度。

12.  产品冷冻干燥成粉末或在0.01 M PBS/ 0.01% 叠氮化钠溶液中，-20℃避光储存。

F/P计算：

Cy3在552nm摩尔吸光系数为150 000M-1 cm-1;此蛋白在280nm的摩尔吸光系数为170 000 M-1 cm-1;

不同蛋白的摩尔吸光系数不一样;.Cy3染料本身在280 nm 的吸收是552nm处的8%。按以下公式计算F/P

值。

[Cy3] = A552/150000

[antibody] = {A280 – (0.08×A552)}/170000

F/P final= [Cy3]/[antibody]= {1.13×A552}/{A280 – (0.08×A552)}

（二）：Cyanine5 NHS ester和标记 (D-ser2)-leucineenkephalin的连接实验方法和步骤

【准备材料】：

Cy5 NHS ester purchased from xi an ruixibio

(D-ser2)-leucineenkephalin purchased fromsigma

【实验步骤】：

1.      Cy5 NHS 1.0 mg 溶解于400 μL DMSO后，加入到1mL 的玻璃瓶中盛有(D-ser2)-leucine-enkephalinacetate(YSGFLT, 0.75 mg) 的400 μL DMSO溶液。(Dye 和peptide 投料比是 1:1)

2.      然后加入15 μL三乙胺，常温避光搅拌反应混合物过夜。

3.      用HPLC 纯化蛋白，使用蛋白C18柱子(25 cm×10 mm)，每次上样注入2×400 μL，30分钟梯度洗脱从0.1% TFA水溶液到MeCN∶H2O(0.1% TFA)=70∶30，流速4mL /min。(对不同的蛋白选择不同的合适HPLC梯度流动相)

4.      收集适当的色带峰，标记多肽的保留时间比未标记的多肽长。

5.      产品冷冻干燥成粉末或在水溶液中，-20℃避光储存;必要时可用质谱表征。

6.      CyDye标记的蛋白稳定性取决于蛋白本身。例如标记的IgG在4 ℃可避光保存2月;更长期的保存需加入等体积的甘油-20 ℃避光保存.

F/P计算：

Cy5 在650 nm 的摩尔吸光系数为250 000 M-1 cm-1 ，所用蛋白在280 nm处的摩尔吸光系数为 170

000 M-1 cm-1;Cy5在280nm处的吸收是650nm处的5%。按以下公式计算F/P值。

[Cy5 dye] = (A650)/250 000

[peptide] =[A280 -(0.05×A650)]/170 000

F/P final= [dye]/[peptide]= {0.68×A650}/{A280 – (0.05×A650)}

 

上海金畔生物科技有限公司产品种类：

Cyanine3 alkyne

Cyanine3 azide

Cyanine3 carboxylic acid  1144107-76-5

Cyanine3 hydrazide

Cyanine3 maleimide

Cyanine3 NHS ester   1032678-38-8

Cyanine3.5 azide

Cyanine3.5 carboxylic acid  1144107-79-8

Cyanine3.5 NHS ester

Cyanine5 alkyne

Cyanine5 amine

Cyanine5 azide

Cyanine5 Boc-hydrazide

Cyanine5 carboxylic acid

Cyanine5 hydrazide

Cyanine5 maleimide

Cyanine5 NHS ester  1032678-42-4

Cyanine5.5 alkyne

Cyanine5.5 azide

Cyanine5.5 carboxylic acid  1144107-80-1

Cyanine5.5 maleimide

Cyanine5.5 NHS ester

Cyanine7 amine   1650635-41-8

Cyanine7 azide

Cyanine7 carboxylic acid

Cyanine7 maleimide

Cyanine7 NHS ester

Cyanine7.5 amine

Cyanine7.5 azide

Cyanine7.5 carboxylic acid   1686147-68-1

Cyanine7.5 maleimide

Cyanine7.5 NHS ester

Sulfo-Cyanine 5 NHS ester

Sulfo-Cyanine3 azide

Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine3 maleimide

Sulfo-Cyanine3 NHS ester

Sulfo-Cyanine5 alkyne

Sulfo-Cyanine5 azide

Sulfo-Cyanine5 carboxylic acid   1121756-16-8

Sulfo-Cyanine5 maleimide

Sulfo-Cyanine7 azide

Sulfo-Cyanine7 carboxylic acid

Sulfo-Cyanine7 NHS ester

Lumiprobe BDP TR azide 23430

发表于由

Lumiprobe BDP TR azide 23430

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商，上海金畔生物Lumiprobe 金畔生物官网：http://lumiprobe.jinpanbio.com/。可提供Lumiprobe的完整产品线，所有规格产品保证货期5-7工作日到货，满足您的各种科研实验需求。Lumiprobe产品包括：羧酸类染料、羰基类染料，标记分子氨基活性染料，标记分子巯基活性染料，动物活体成像用染料，蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规（市场上部分是分装，质量不能保证），质量保证，价格极具竞争力,而且我们的货期仅需要5-7工作日，不需要像其他家攒单导致货期3-4周。

服务热线:18301939375   QQ：3258089810    3259632176

This is an azide for copper catalyzed and copper free Click chemistry labeling with BDP TR, a bright red emitting fluorophore. BDP TR is an alternative to ROX and Texas Red. This fluorophore is based on borondipyrromethene scaffold, and contains aryl substituents to match its absorption and emission to ROX/Texas Red channel.

ROX and Texas Red dyes are prone to oxidation, BDP TR is much more resilient to oxidants. Its brightness and photostABIlity is also very high.

Lumiprobe BDP TR azide 23430-货期5个工作日

General properties

Appearance: dark colored solid
Mass spec M+ increment: 506.2
Molecular weight: 506.34
Molecular formula: C24H21N6BF2O2S
Solubility: good in DMF, DMSO, DCM
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 589
ε, L⋅mol−1⋅cm−1: 69000
Emission maximum, nm: 616
Fluorescence quantum yield: 0.9
CF260: 0.15
CF280: 0.19

上海金畔生物科技有限公司（www.jinpanbio.com）提供生命科学研究领域系列产品，包括生化试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料；修饰性PEG（Laysan bio、NANOCS、Avanti等进口品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG）；Sigma、Amresco、TCI、MP bio生化试剂；WAKO日本和光纯药；日本关东化学Kanto试剂；Megazyme食品分析检测试剂盒、Research diets品牌的动物饲料；Oxoid、Nissui日水、BD difco、Himedia品牌微生物培养基；免疫试剂包括:Abcam、CST、Santa Cruz、Bethyl抗体；Biolegend流式抗体；Roche、TOYOBO、NEB品牌的酶；中检所、TRC、药典USP、EP、Reagecon标准品；耗材和仪器包括Whatman、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、老鼠软管灌胃针、动物毛发记号笔、Labnet、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。

 

Lumiprobe 羧基类、羰基类荧光染料

发表于由

Lumiprobe 羧基类、羰基类荧光染料

Lumiprobe Corporation是美国一家高品质生物技术公司，专业提供分子生物学研究用的活性荧光染料。从2006年开始，公司生产并销售生命科学研究和诊断学应用的优质化学药品。产品主要有：活性染料(Reactive Dye)和SYBR Green I 染料，用于寡核苷酸合成的亚磷酰胺，点击化学用染料和其它试剂。

产品主要应用：点击化学(Click Chemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2D DIGE)和实时荧光定量PCR(Realtime PCR)。氨基类染料是包含自由氨基的活性染料，染料可与活化羧酸衍生物和其他亲电子的试剂结合。比如：氨基与EDC-活化的羧基结合。

羧基类、羰基类染料解决方案----Lumiprobe实验室通用技术方案

上海金畔生物科技有限公司是Lumiprobe一级代理，可提供Lumiprobe完善的产品线和具有竞争力的价格，包括:羧酸类染料、羰基类染料，标记分子氨基活性染料，标记分子巯基活性染料，动物活体成像用染料，蛋白质、多肽、核酸等生物分子标记染料等。
产品线
羧酸类染料
羧酸类染料为未活化自由酸染料。
产品 分子结构 规格
编号：AGF1324A
Cy3羧酸
Cy3 carboxylic acid		 羧基类、羰基类染料解决方案----Lumiprobe实验室通用技术方案 1mg，5mg，25mg，50mg，100mg
编号：AGF1335A
Cy5羧酸
Cy5 carboxylic acid		 羧基类、羰基类染料解决方案----Lumiprobe实验室通用技术方案 1mg，5mg，25mg，50mg，100mg
编号：AGF1343A
Cy5.5羧酸
Cy5.5 carboxylic acid		 羧基类、羰基类染料解决方案----Lumiprobe实验室通用技术方案 1mg，5mg，25mg，50mg，100mg
编号：AGF1347A
Cy7羧酸
Cy7 carboxylic acid		 羧基类、羰基类染料解决方案----Lumiprobe实验室通用技术方案 1mg，5mg，25mg，50mg，100mg
编号：AGF1354A
Cy7.5羧酸
Cy7.5 carboxylic acid		 羧基类、羰基类染料解决方案----Lumiprobe实验室通用技术方案 1mg，5mg，25mg，50mg，100mg
编号：AGF1376A
磺酸基-cy5 羧酸
Sulfo-Cy5 carboxylic acid		 羧基类、羰基类染料解决方案----Lumiprobe实验室通用技术方案 1mg，5mg，25mg，50mg，100mg
编号：AGF1378A
磺酸基-cy7 羧酸
Sulfo-Cy7 carboxylic acid		 羧基类、羰基类染料解决方案----Lumiprobe实验室通用技术方案 1mg，5mg，25mg，50mg，100mg
羰基类染料
羰基活性染料用于标记生物分子中的醛和酮。这类染料可与生物分子的羰基温和地发生反应。比如对氧敏感的蛋白，可被高碘酸盐氧化作用激活的糖蛋白和带有羰基的寡核苷酸。
产品 分子结构 可替代染料
编号：AGF1336A
中文名：Cy5 酰肼
英文名：Cy5 hydrazide		 羧基类、羰基类染料解决方案----Lumiprobe实验室通用技术方案 Carbonyl-reactive Alexa Fluor 647
Carbonyl-reactive DyLight 649
编号：AGF1334A
中文名：Cy5 Boc-酰肼
英文名：Cy5 Boc-hydrazide		 羧基类、羰基类染料解决方案----Lumiprobe实验室通用技术方案

Lumiprobe 磺酸基-Cy5-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine5 NHS ester

发表于由

Lumiprobe 磺酸基-Cy5-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine5 NHS ester

水溶Cyanine 5琥珀酰亚胺酯（succinimidyl ester（SE）），类似于Cy5® NHS ester，不需要有机溶剂即在水相中即可对各种带氨基分子进行标记。因此该产品尤其适用于对那些在有机溶剂中易变性的蛋白和低溶解度蛋白的标记。Sulfo-Cyanine 5是Cy5®的衍生物，与很多仪器如读板机（plate readers），显微镜和成像设备等相兼容。是最受欢迎的荧光染料之一。
该染料是高度亲水的、水溶的。非磺化的衍生物：Cyanine 5 NHS ester。可以在所有的应用中替代Cy5®，Alexa Fluor 647和DyLight 649。

Lumiprobe 磺酸基-Cy5-N-羟基琥珀酰亚胺酯 Sulfo-Cyanine5 NHS ester   一般特性

外观: dark blue powder
分子量: 761.84
分子式: C36H40N3NaO10S2
溶解性: very good in water, good in DMF and DMSO
质量控制: NMR 1H (95%) and 13C, TLC, functional testing
储存条件: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

光谱特性

最大激发波长, nm: 646
在最大激发波长下的消光系数, Lmol-1cm-1: 271000
最大发生波长, nm: 662
荧光量子产率: 0.2
CF260: 0.13
CF280: 0.13

Cy5 NHS ester structure

Sulfo-Cyanine5 NHS ester

上海金畔生物科技有限公司还可以提供其他相关产品：

Cyanine3 alkyne

Cy3 alkyne

Cyanine3 azide

Cy3 azide

Cy3 N3

Cyanine3 carboxylic acid  1144107-76-5

Cy3 carboxylic acid

Cyanine3 hydrazide

Cyanine3 maleimide

Cy3 maleimide

Cyanine3 NHS ester   1032678-38-8

Cy3 NHS ester

Cyanine3.5 azide

Cyanine3.5 carboxylic acid  1144107-79-8

Cyanine3.5 NHS ester

Cy3.5 NHS ester

Cyanine5 alkyne

Cyanine5 amine

Cy5 amine

Cy5 NH2

Cyanine5 azide

Cy5 N3

Cyanine5 Boc-hydrazide

Cyanine5 carboxylic acid

Cyanine5 hydrazide

Cyanine5 maleimide

Cy5 maleimide

Cyanine5 NHS ester  1032678-42-4

Cy5 NHSester

Cyanine5.5 alkyne

Cyanine5.5 azide

Cyanine5.5 carboxylic acid  1144107-80-1

Cyanine5.5 maleimide

Cyanine5.5 NHS ester

Cyanine7 amine   1650635-41-8

Cy7 amine

Cy7 NH2

Cyanine7 azide

Cy7 azide

Cyanine7 carboxylic acid

Cyanine7 maleimide

Cy7 maleimide

Cyanine7 NHS ester

Cy7 NHS ester

Cyanine7.5 amine

Cyanine7.5 azide

Cyanine7.5 carboxylic acid   1686147-68-1

Cyanine7.5 maleimide

Cyanine7.5 NHS ester

Sulfo-Cyanine 5 NHS ester

Sulfo-Cy 5 NHS ester

Sulfo-Cyanine3 azide

Sulfo-Cy3 azide

Sulfo-Cyanine3 carboxylic acid

Sulfo-Cyanine3 maleimide

Sulfo-Cy3 maleimide

Sulfo-Cyanine3 NHS ester

Sulfo-Cy3 NHS ester

Sulfo-Cyanine5 alkyne

Sulfo-Cyanine5 azide

Sulfo-Cy5 azide

Sulfo-Cyanine5 carboxylic acid   1121756-16-8

Sulfo-Cyanine5 maleimide

Sulfo-Cy5 maleimide

Sulfo-Cyanine7 azide

Sulfo-Cyanine7 carboxylic acid

Sulfo-Cyanine7 NHS ester

Sulfo-Cy7 NHS ester