标签归档：lumiprobe cy荧光染料

Lumiprobe蛋白质组学试剂 Proteomic reagents

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Lumiprobe蛋白质组学试剂 Proteomic reagents

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商，上海金畔生物Lumiprobe官网：http://lumiprobe.jinpanbio.com/。可提供Lumiprobe的完整产品线，所有规格产品保证货期5-7工作日到货，满足您的各种科研实验需求。Lumiprobe产品包括：羧酸类染料、羰基类染料，标记分子氨基活性染料，标记分子巯基活性染料，动物活体成像用染料，蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规（市场上部分是分装，质量不能保证），质量保证，价格极具竞争力,而且我们的货期仅需要5-7工作日，不需要像其他家攒单导致货期3-4周。

Lumiprobe蛋白质组学试剂 Proteomic reagents

Cyanine2 NHS ester minimal dye

Cyanine2 NHS ester minimal dye for protein labeling

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1A041 5 nmol
2A041 10 nmol
3A041 25 nmol

Cyanine3 NHS ester minimal dye

Cyanine3 minimal dye for protein labeling.

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1B041 5 nmol
2B041 10 nmol
3B041 25 nmol

Cyanine5 NHS ester minimal dye

Cy5 NHS ester minimal dye for protein labeling.

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1C041 5 nmol
2C041 10 nmol
3C041 25 nmol

产品列表

货号
中文名
产品描述
1A041 Cy2-N-羟基琥珀酰亚胺酯 Cyanine2 NHS ester minimal dye, 5 nmol
2A041 Cy2-N-羟基琥珀酰亚胺酯 Cyanine2 NHS ester minimal dye, 10 nmol
3A041 Cy2-N-羟基琥珀酰亚胺酯 Cyanine2 NHS ester minimal dye, 25 nmol
1B041 Cy3-N-羟基琥珀酰亚胺酯 Cyanine3 NHS ester minimal dye, 5 nmol
2B041 Cy3-N-羟基琥珀酰亚胺酯 Cyanine3 NHS ester minimal dye, 10 nmol
3B041 Cy3-N-羟基琥珀酰亚胺酯 Cyanine3 NHS ester minimal dye, 25 nmol
1C041 Cy5-N-羟基琥珀酰亚胺酯 Cyanine5 NHS ester minimal dye, 5 nmol
2C041 Cy5-N-羟基琥珀酰亚胺酯 Cyanine5 NHS ester minimal dye, 10 nmol
3C041 Cy5-N-羟基琥珀酰亚胺酯 Cyanine5 NHS ester minimal dye, 25 nmol

* 上述中文名称仅供参考,订货以货号和英文名称为准

辅助试剂 Auxiliary reagents
抗坏血酸 Ascorbic acid

ascorbic acid for reduction of Cu(II)-TBTA complex.

Ascorbic acid
铜-TBTA络合物，10mM溶于55%的二甲亚砜水溶液
Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO

Catalyst for bioconjugate synthesis by Click Chemistry.

DMF (dimethylformamide)
标记级别二甲基酰胺 DMF (dimethylformamide), labeling grade

Dry high purity amine free solvent for NHS ester labeling.


产品列表

货号
中文名
产品描述
1A041 Cy2-N-羟基琥珀酰亚胺酯 Cyanine2 NHS ester minimal dye, 5 nmol
12050 抗坏血酸 Ascorbic acid, 10 mg
21050 铜-TBTA络合物，10mM溶于55%的二甲亚砜水溶液 Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO, 1.5 mL
13050 标记级别二甲基酰胺 DMF (dimethylformamide), labeling grade, 1 mL

* 上述中文名称仅供参考,订货以货号和英文名称为准

PCR/qPCR PCR/qPCR
罗丹明对照品染料用于快速PCR ROX reference dye for qPCR

ROX is a passive reference dye for qPCR amplification. The dye can be used to normalize fluorescence intensity of reporter dye in qPCR.

SYBR Green I 染料用于实时PCR SYBR Green I for Real Time PCR, 100x

SYBR Green I stock solution for Real Time PCR, 100x.

SYBR Green I 凝胶染色溶液 SYBR Green I Gel Staining Solution, 10000x

SYBR Green I dye for staining dsDNA in gelen.


产品列表

货号
中文名
产品描述
31110 罗丹明对照品染料用于快速PCR ROX reference dye for qPCR, 500 uL
41010 SYBR Green I 染料用于实时PCR SYBR Green I for Real Time PCR, 100x, 1 mL
51010 SYBR Green I 染料用于实时PCR SYBR Green I for Real Time PCR, 100x, 1.5 mL
51010-5 SYBR Green I 染料用于实时PCR SYBR Green I for Real Time PCR, 100x, 5×1.5 mL
20010 SYBR Green I 凝胶染色溶液 SYBR Green I Gel Staining Solution, 10000x, 1 mL

Lumiprobe点击化学用染料-非荧光炔烃 Non-fluorescent alkynes

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Lumiprobe点击化学用染料-非荧光炔烃 Non-fluorescent alkynes

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商，上海金畔生物Lumiprobe官网：http://lumiprobe.jinpanbio.com/。可提供Lumiprobe的完整产品线，所有规格产品保证货期5-7工作日到货，满足您的各种科研实验需求。Lumiprobe产品包括：羧酸类染料、羰基类染料，标记分子氨基活性染料，标记分子巯基活性染料，动物活体成像用染料，蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规（市场上部分是分装，质量不能保证），质量保证，价格极具竞争力,而且我们的货期仅需要5-7工作日，不需要像其他家攒单导致货期3-4周。

非荧光炔烃 Non-fluorescent alkynes
生物素-炔烃 Biotin alkyne

Biotin alkyne is a reactive affinity label which can be attached to biomolecules via Click Chemistry.

Biotin alkyne
戊炔酸 STP酯 Pentynoic acid STP ester

An activated ester for the introduction of alkyne into peptides/proteins.

Pentynoic acid STP ester

产品列表

货号
中文名
产品描述
C37B0 生物素-炔烃 Biotin alkyne, 10 mg
D37B0 生物素-炔烃 Biotin alkyne, 25 mg
E37B0 生物素-炔烃 Biotin alkyne, 50 mg
F37B0 生物素-炔烃 Biotin alkyne, 100 mg
30720 戊炔酸 STP酯 Pentynoic acid STP ester, 10 mg
40720 戊炔酸 STP酯 Pentynoic acid STP ester, 25 mg
50720 戊炔酸 STP酯 Pentynoic acid STP ester, 50 mg
60720 戊炔酸 STP酯 Pentynoic acid STP ester, 100 mg

* 上述中文名称仅供参考,订货以货号和英文名称为准

Lumiprobe点击化学用染料 –无荧光叠氮化物 Non-fluorescent azides

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Lumiprobe点击化学用染料 –无荧光叠氮化物 Non-fluorescent azides

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商，上海金畔生物Lumiprobe官网：http://lumiprobe.jinpanbio.com/。可提供Lumiprobe的完整产品线，所有规格产品保证货期5-7工作日到货，满足您的各种科研实验需求。Lumiprobe产品包括：羧酸类染料、羰基类染料，标记分子氨基活性染料，标记分子巯基活性染料，动物活体成像用染料，蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规（市场上部分是分装，质量不能保证），质量保证，价格极具竞争力,而且我们的货期仅需要5-7工作日，不需要像其他家攒单导致货期3-4周。

非荧光叠氮化物 Non-fluorescent azides
氨基丁酸N-羟基琥珀酰亚胺酯 Azidobutyric acid NHS ester

Activated ester for azido labeling of peptides and proteins.

Azidobutyric acid NHS ester
生物素-叠氮化物 Biotin azide

Biotin azide is a reagent for click chemistry labeling with biotin, a well-known affinity probe.

Biotin azide

产品列表

货号
中文名
产品描述
33720 氨基丁酸N-羟基琥珀酰亚胺酯 Azidobutyric acid NHS ester, 10 mg
43720 氨基丁酸N-羟基琥珀酰亚胺酯 Azidobutyric acid NHS ester, 25 mg
53720 氨基丁酸N-羟基琥珀酰亚胺酯 Azidobutyric acid NHS ester, 50 mg
C3730 生物素-叠氮化物 Biotin azide, 10 mg
D3730 生物素-叠氮化物 Biotin azide, 25 mg
E3730 生物素-叠氮化物 Biotin azide, 50 mg
F3730 生物素-叠氮化物 Biotin azide, 100 mg

* 上述中文名称仅供参考,订货以货号和英文名称为准

Lumiprobe FAM azide, 6- isomer染料

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Lumiprobe FAM azide, 6- isomer染料

FAM azide有两种形式可供选择，粉剂和10mM浓度溶于DMSO的溶液。用于点击化学的试剂标记。该产品是纯化的6-isomer。可以替代Alexa Fluor 488和DyLight 488。

FAM (fluorescein) azide. solid and 10 mM solution in DMSO, labeling reagent for Click chemistry.

Pure 6-isomer.

Can replace Alexa Fluor 488, DyLight 488.

6-FAM azide structure

订购货号 产品名称及规格
15130 FAM azide, 6- isomer, 100 uL, 10 mM/DMSO
35130 FAM azide, 6- isomer, 500 uL, 10 mM/DMSO
45130 FAM azide, 6- isomer, 1 mL, 10 mM/DMSO
A5130 FAM azide, 6- isomer, 1 mg
B5130 FAM azide, 6- isomer, 5 mg
C5130 FAM azide, 6- isomer, 10 mg
D5130 FAM azide, 6- isomer, 25 mg
E5130 FAM azide, 6- isomer, 50 mg

General properties

Appearance: yellowish crystals
Molecular weight: 458.42
Molecular formula: C24H18N4O6
Solubility: soluble in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download

Spectral properties

Excitation maximum, nm: 494
Extinction coefficient at excitation maximum, Lmol-1cm-1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9

 

Coumarin 343 azide荧光染料

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Coumarin 343 azide荧光染料

Lumiprobe Corporation是美国一家高品质生物技术公司，专业提供分子生物学研究用的活性荧光染料。从2006年开始，公司生产并销售生命科学研究和诊断学应用的优质化学药品。产品主要有：活性染料(Reactive Dye)和SYBR Green I 染料，用于寡核苷酸合成的亚磷酰胺，点击化学用染料和其它试剂。 产品主要应用：点击化学(Click Chemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2D DIGE)和实时荧光定量PCR(Realtime PCR)。其中，氨基类染料是包含自由氨基的活性染料，染料可与活化羧酸衍生物和其他亲电子的试剂结合。比如：氨基与EDC-活化的羧基结合。

 

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商，上海金畔生物Lumiprobe官网：http://lumiprobe.jinpanbio.com/。可提供Lumiprobe的完整产品线，所有规格产品保证货期5-7工作日到货，满足您的各种科研实验需求。Lumiprobe产品包括：羧酸类染料、羰基类染料，标记分子氨基活性染料，标记分子巯基活性染料，动物活体成像用染料，蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规（市场上部分是分装，质量不能保证），质量保证，价格极具竞争力,而且我们的货期仅需要5-7工作日，不需要像其他家攒单导致货期3-4周。

Coumarin 343 azide荧光染料

This product will be discontinued soon in favor of Coumarin 343 X azide. We may still have some material in stock (please see availability above).

This greenish-blue emitting fluorophore can be used as a FRET donor for FAM. Azide derivative is suitable for Click Chemistry.Coumarin 343 azide structure

该蓝绿色光荧光染料可以用于FAM的FRET供体。叠氮类衍生物可以用于点击化学。

订购货号 产品名称及规格
A1430 Coumarin 343 azide, 1 mg
B1430 Coumarin 343 azide, 5 mg
C1430 Coumarin 343 azide, 10 mg
D1430 Coumarin 343 azide, 25 mg
E1430 Coumarin 343 azide, 50 mg

General properties

Appearance: yellow powder
Molecular weight: 441.48
Molecular formula: C22H27N5O5
Solubility: Good in organic solvents (DCM, chloroform, DMF, DMSO, MeCN, alcohols), poor in water
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download

Spectral properties

Excitation maximum, nm: 437
Extinction coefficient at excitation maximum, Lmol-1cm-1: 44000
Emission maximum, nm: 477
Fluorescence quantum yield: 0.63

Cy7-N-羟基琥珀酰胺酯|CAS 477908-53-5|Sulfo-cyanine7 NHS ester

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Cy7-N-羟基琥珀酰胺酯|CAS 477908-53-5|Sulfo-cyanine7 NHS ester

产品简介

胺类活性染料，近红外荧光染料，Cy7?的改进的类似物。近红外荧光素可以被用来利用生物组织的近红外窗口。在这个光谱区域，高度增强的组织透明度使得活体成像成为可能。该染料可用于制备CY7标记的生物分子来进行后续试验，如活体研究及药物开发相关实验。Cy7的特殊结构加固了中心聚亚甲基（polymethyne）链的稳定性。这种增强的分子结构会使其量子产率较其母结构提供20%，因此它的荧光强度会更大。

该试剂在标记时需要共溶剂有机溶剂。水溶Cy7 NHS ester可以用于蛋白NIR标记。

Amine reactive Cyanine7, near infrared fluorescent dye, an improved analog of Cy7®.

NIR fluorophores can be used to take advantage of near infrared window of biological tissues – increased transparency of tissues in this spectral region allows to carry out in vivo imaging.

This reagent can be utilized to produce Cyanine7-labeled biomolecules for subsequent use in various in vivo research, and drug design related experiments.

The structure of Cyanine7 features rigidized design of central polymethyne chain. This molecular reinforcement allows to increase quantum yield by 20% compared with parent structure, increasing fluorescence brightness.

This reagent requires organic co-solvent for the labeling (please see Recommended Protocols section below). Water-soluble Cyanine7 NHS ester is also available, and recommended for protein NIR labeling.

一般特性

 外观: green powder
分子量: 682.29
分子式: C41H48ClN3O4
溶解性: soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility   in water
质量控制: NMR 1H (95%) and 13C, TLC, functional testing
储存条件: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for   up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

光谱特性

激发波长, nm: 750
在激发波长下的消光系数, Lmol-1cm-1: 199000
发生波长, nm: 773
荧光量子产率: 0.3

Lumiprobe ROX NHS ester, pure 6- isomer

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Lumiprobe ROX NHS ester, pure 6- isomer

ROX(rhodamine X aka R101)是一种高荧光强度的、耐光的罗丹明染料，在多领域有广泛的应用。ROX标记的寡核苷酸探针常用于qPCR和有ROX通道的qPCR仪器。
该活性染料用于标记多肽、蛋白、氨基寡核苷酸的氨基基团。Pure single isomer.
ROX NHS ester可以替代Alexa Fluor 568。

ROX (rhodamine X aka R101) is a highly fluorescent, and photostable rhodamine dye for various applications. ROX labeled oligonucleotide probes are often used in qPCR, and qPCR instruments have ROX channel.

This is reactive dye for the labeling of amino-groups in peptides, proteins, and amino-oligonucleotides. Pure single isomer.

This dye can replace Alexa Fluor 568, Texas Red.

Absorption and emission spectra of ROX dyeROX NHS ester, pure 6- isomer

订购货号 产品名称及规格
12220 ROX NHS ester, pure 6- isomer, 1 mg
22220 ROX NHS ester, pure 6- isomer, 5 mg
42220 ROX NHS ester, pure 6- isomer, 25 mg
52220 ROX NHS ester, pure 6- isomer, 50 mg
62220 ROX NHS ester, pure 6- isomer, 100 mg

General properties

Appearance: dark crimson powder
Mass spec M+ increment: 516.2
Molecular weight: 631.67
Molecular formula: C37H33N3O7
CAS number: 117491-83-5
Solubility: good in polar organic solvents (DMF, DMSO), low in water
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 570
Extinction coefficient at excitation maximum, Lmol-1cm-1: 82000
Emission maximum, nm: 591
Fluorescence quantum yield: 1.0

Cy5.5 NHS ester 氨基反应性荧光染料,不溶于水

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Cy5.5 NHS ester 氨基反应性荧光染料,不溶于水

Cy5.5 NHS是近红外胺类活性染料。CY5.5 NHS ester是用于标记多肽、蛋白和寡核苷酸的氨基基团的活性染料。CY5.5属于是近红外荧光染料，当背景荧光有干扰时适宜用它来做标记以进行荧光分析。它也可以用于活体成像实验。该染料可以替代Alexa Fluor 680和DyLight 680。

Cyanine5.5 NHS ester是用于标记含有伯胺的多肽，蛋白和寡核苷酸的反应性染料。 Cy5.5-NHS ester是一种近红外荧光染料，能够溶解在DMF和DMSO中，最大激发光和最大发射光分别在675 nm和693 nm左右[1]。该试剂不仅用于标记含有伯基的分子，而且还用于标记质粒DNA [2]。在生物分子标记中，该标记试剂的水溶性较低，先使用有机溶剂溶解该分子对于高效标记是必需的。首先，Cyanine染料应该溶解在有机溶剂中，然后加入包含生物分子的合适缓冲水溶液中。

通过皮下移植肿瘤小鼠的光学成像，在0.5nmol Cy5.5-NHS注射后30min至24h，皮下U87MG肿瘤可与周围背景组织明显区分开来。 Cy5.5-NHS的肿瘤吸收在注射后30分钟达到最大值，随着时间的推移缓慢洗出[3]。

Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester.

Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments.

This reagent can replace NHS esters of Cy5.5®, Alexa Fluor 680, and DyLight 680.

Cy5.5 NHS ester structureCyanine5.5 NHS ester

General properties

Appearance: dark blue powder
Molecular weight: 716.31
Molecular formula: C44H46ClN3O4
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 673
Extinction coefficient at excitation maximum, Lmol-1cm-1: 209000
Emission maximum, nm: 707
Fluorescence quantum yield: 0.2

氨基分子的NHS酯标记

NHS (N-HydroxySuccinimide) esters and other activated esters (sulfo-NHS, sulfotetrafluorophenyl – STP) are reactive compounds suitable for the modification of amino groups. NHS is most common type of activated esters.

Usual modifications are fluorescent labels, fluorescence quenchers, and other reporter groups. Alkyne and azido group can be attached using activated esters to adapt biomolecules to Click Chemistry.

Since amino groups are nearly always contained in proteins and peptides, modification of these biopolymers is especially common. Other examples are amino-oligonucleotides, amino-modified DNA, and amino-containing sugars.

The reaction of NHS esters with amines is strongly pH-dependent: at low pH, the amino group is protonated, and no modification takes place. At higher-than-optimal pH, hydrolysis of NHS ester is quick, and modification yield diminishes. Optimal pH value for modification is 8.3-8.5.

Water is most common solvent for the labeling. If NHS ester is poorly soluble, it can be added as a solution in DMSO or DMF to a solution of protein in water, adjusted to pH 8.3-8.5. Note that DMF must not contain amines (and thus should have no odor).

We recommend using the following general protocol for the labeling of biomolecules with NHS esters produced by Lumiprobe.

  1. Calculate required amount of NHS ester:NHS_ester_weight [mg] = 8 × amino_compound_weight [mg] × NHS_ester_molar_weight [Da] / amino_compound_molar_weight [Da].

    8 is molar excess of NHS ester. It is experimental value for mono-labeling, suitable for many common proteins and peptides. However, in some cases using less or more NHS ester is required. It depends on protein structure, reagent, and solubility. Molar weight of Lumiprobe products can be found on corresponding product pages.

    For example, to label 3 mg of insulin (molar weight 69300 Dalton) with Cy5 NHS ester (molar weight 592 Dalton), and obtain maximum yield of mono-labeled product, one should use
    10 × 3 mg × 592 Da / 69300 Da = 0.26 mg
    of Cy5 dye NHS ester.

  2. Determine volume of reaction mixture. The labeling can be performed on any scale from nanomols to dozens of grams. When the scale is low, use minimal volume (10-20 uL). Higher concentrations (1-10 mg of amino-biomolecule per mL of mixture) are optimal.
  3. Dissolve NHS ester in 1/10 reaction volume of DMF or DMSO. Amine-free DMF is preferred solvent. After the reaction, NHS ester can be stored in solution for 1-2 months at -20ºC.
  4. Dissolve biomolecule in 9/10 reaction volume of buffer with pH 8.3-8.5.0.1 M Sodium bicarbonate solution has appropriate pH. Other alternatives are 0.1 M Tris buffer (although Tris has amino group, it is hindered and does not react with NHS esters), or 0.1 M phosphate buffer. Note pH is the most important thing.

    When doing large-scale labeling (hundreds of milligrams of NHS ester), note that the mixture tends to acidify with time because of hydrolysis of NHS ester. Monitor pH, or use more concentrated buffer then.

  5. Add NHS ester solution to the solution of biomolecule, and vortex well. Keep on ice overnight, or at room temperature during at least 4 hours.
  6. Purify the conjugate using appropriate method: gel-filtration for macromolecules is most universal. Precipitation and chromatography is another alternative. Organic impurities (such as N-hydroxysuccinimide, NHS ester, acid produced by hydrolysis) are almost always easily separated. For proteins and nucleic acids, ethanol or acetone precipitation can be used.

Lumiprobe Cy3.5 NHS ester 氨基反应性荧光染料,不溶于水

发表于由

Lumiprobe Cy3.5 NHS ester 氨基反应性荧光染料,不溶于水

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商，上海金畔生物Lumiprobe官网：http://lumiprobe.jinpanbio.com/。可提供Lumiprobe的完整产品线，所有规格产品保证货期5-7工作日到货，满足您的各种科研实验需求。Lumiprobe产品包括：羧酸类染料、羰基类染料，标记分子氨基活性染料，标记分子巯基活性染料，动物活体成像用染料，蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规（市场上部分是分装，质量不能保证），质量保证，价格极具竞争力,而且我们的货期仅需要5-7工作日，不需要像其他家攒单导致货期3-4周。

服务热线:18301939375   QQ：3258089810    3259632176

Cyanine3.5 NHS ester (an analog of Cy3.5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.

Cyanine3.5 NHS ester can replace NHS esters of Cy3.5®, Alexa Fluor 594, DyLight 594.

订购货号 产品名称及规格
12020 Cyanine3.5 NHS ester, 1 mg
22020 Cyanine3.5 NHS ester, 5 mg
42020 Cyanine3.5 NHS ester, 25 mg
52020 Cyanine3.5 NHS ester, 50 mg
62020 Cyanine3.5 NHS ester, 100 mg

General properties

Appearance: dark purple powder
Molecular weight: 690.27
Molecular formula: C42H44ClN3O4
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H and HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

 

Excitation maximum, nm: 591
Extinction coefficient at excitation maximum, Lmol-1cm-1: 116000
Emission maximum, nm: 604
Fluorescence quantum yield: 0.35

 

Product citations

 

  • Wang, C.; Niederstrasser, H.; Douglas, P.M.; Lin, R.; Jaramillo, J.; Li, Y.; Olswald, N.W.; Zhou, A.; McMillan, E.A.; Mendiratta, S.; Wang, Z.; Zhao, T.; Lin, Z.; Luo, M.; Huang, G.; Brekken, R.A.; Posner, B.A.; MacMillan, J.B.; Gao, J.; White, M.A. Small-molecule TFEB pathway agonists that ameliorate metabolic syndrome in mice and extend C. elegans lifespan. Nature Communications, 2017, 8, 2270. doi: 10.1038/s41467-017-02332-3
  • Massey, M.; Kim, H.; Conroy, E.M.; Algar, W.R. Expanded Quantum Dot-Based Concentric Förster Resonance Energy Transfer: Adding and Characterizing Energy-Transfer Pathways for Triply Multiplexed Biosensing. The Journal of Physical Chemistry C, 2017, 121(24), 13345–13356. doi: 10.1021/acs.jpcc.7b02739
  • Wang, C.; Wang, Y.; Li, Y.; Bodemann, B.; Zhao, T.; Ma, X.; Huang, G.; Hu, Z.; DeBerardinis, R.J.; White, M.A.; Gao, J. A nanobuffer reporter library for fine-scale imaging and perturbation of endocytic organelles. Nature Communications, 2015, 6, 8524. doi:10.1038/ncomms9524
  • Fu, B.; Flynn, J.D.; Isaacoff, B.P.; Rowland, D.J.; Biteen, J.S. Super-Resolving the Distance-Dependent Plasmon-Enhanced Fluorescence of Single Dye and Fluorescent Protein Molecules. The Journal of Physical Chemistry C, 2015, 119(33), 19350–19358. doi: 10.1021/acs.jpcc.5b05154

 

Lumiprobe Sulfo-Cyanine7 NHS ester

发表于由

Lumiprobe Sulfo-Cyanine7 NHS ester

Sulfo-Cyanine7 NHS ester是水溶近红外染料sulfo-Cyanine7，是胺类活性琥珀酸亚胺酯。

Sulfo-Cyanine7是Cy7®荧光团的的改良衍生物，量子产率提高20%，并具有更好的耐光性。该染料尤其适用于NIR成像应用。

近红外荧光染料利用了生物组织在特定的波长范围里的透明度。这种方法是无损伤性的，并允许在活体内对多种已标记的生物分子的分布进行跟踪。

Sulfo-Cyanine7 NHS ester可以很轻松的标记生物分子，如：蛋白质。标记的分子可以用于后续多种研究及药物开发相关的实验。

这种染料有很高的水溶解度，尤其适用于痕量蛋白质和易变性蛋白质的标记。需有机溶剂才能溶解的非磺化的Cyanine7 NHS ester。

订购货号 产品名称及规格 价格（￥）
15320 Sulfo-Cyanine7 NHS ester, 1 mg 2080.00
25320 Sulfo-Cyanine7 NHS ester, 5 mg 5330.00
45320 Sulfo-Cyanine7 NHS ester, 25 mg 11050.00
55320 Sulfo-Cyanine7 NHS ester, 50 mg 19500.00
65320 Sulfo-Cyanine7 NHS ester, 100 mg 31850.00

General properties

Appearance: dark green powder
Molecular weight: 827.94
Molecular formula: C41H46N3NaO10S2
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

 

Excitation maximum, nm: 740
Extinction coefficient at excitation maximum, Lmol-1cm-1: 240600
Emission maximum, nm: 773
CF260: 0.04
CF280: 0.04

Product citations

  • Ayala-Orozco, C.; Liu, J.G.; Knight, M.W.; Wang, Y.; Day, J.K.; Nordlander, P.; Halas, N.J. Fluorescence Enhancement of Molecules Inside a Gold Nanomatryoshka. Nano Letters, 2014, 14(5), 2926-2933. doi: 10.1021/nl501027j
  • Brand, C.; Abdel-Atti, D.; Zhang, Y.; Carlin, S.; Clardy, S.M.; Keliher, E.J.; Weber, W.A.; Lewis, J.S.; Reiner, T. In Vivo Imaging of GLP-1R with a Targeted Bimodal PET/Fluorescence Imaging Agent. Bioconjugate Chemistry, 2014, 25(7), 1323-1330. doi:10.1021/bc500178d
  • Graen, T.M.D.; Hoefling, M.; Grubmüller, H. AMBER-DYES: Characterization of Charge Fluctuations and Force Field Parameterization of Fluorescent Dyes for Molecular Dynamics Simulations. Journal of Chemical Theory and Computation, 2014, 10(12), 5505-5512. doi: 10.1021/ct500869p
  • Li, L.-L.; Xu, J.-H.; Qi, G.-B.; Zhao, X.; Yu, F.; Wang, H. Core-Shell Supramolecular Gelatin Nanoparticles for Adaptive and “On-Demand” Antibiotic Delivery. ACS Nano, 2014, 8(5), 4975-4983. doi: 10.1021/nn501040h
  • Viehweger, K.; Barbaro, L.; García, K.P.; Joshi, T.; Geipel, G.; Steinbach, J.; Stephan, H.; Spiccia, L.; Graham, B. EGF Receptor-Targeting Peptide Conjugate Incorporating a Near-IR Fluorescent Dye and a Novel 1,4,7-Triazacyclononane-Based 64Cu(II) Chelator Assembled via Click Chemistry. Bioconjugate Chemistry, 2014, 25(5), 1011-1022. doi: 10.1021/bc5001388
  • Zhou, Z.; Wu, X.; Kresak, A.; Griswold, M.; Lu, Z.-R. Peptide targeted tripod macrocyclic Gd(III) chelates for cancer molecular MRI.Biomaterials, 2013, 34(31), 7683-7693. doi: 10.1016/j.biomaterials.2013.06.057
  • Zou, C.; Loka, R.S.; Zhang, Y.; Cairo, C.W. Glycoform Remodeling Generates a Synthetic T Cell Phenotype. Bioconjugate Chemistry, 2013, 24(6), 907-914. doi: 10.1021/bc300599w
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  2. Aldeek, F.; Hawkins, D.; Palomo, V.; Safi, M.; Palui, G.; Dawson, P.E.; Alabugin, I.V.; Mattoussi, H. UV and Sunlight Driven Photoligation of Quantum Dots: Understanding the Photochemical Transformation of the Ligands. Journal of the American Chemical Society, 2015. doi: 10.1021/ja512802x
  3. Hsueh, P.-Y.; Edman, M.C.; Sun, G.; Shi, P.; Xu, S.; Lin, Y.-a.; Cui, H.; Hamm-Alvarez, S.F.; MacKay, J.A. Tear-mediated delivery of nanoparticles through transcytosis of the lacrimal gland. Journal of Controlled Release, 2015. doi: 10.1016/j.jconrel.2014.12.017
  4. Wen, A.M.; Infusino, M.; De Luca, A.; Kernan, D.L.; Czapar, A.E.; Strangi, G.; Steinmetz, N.F. Interface of Physics and Biology: Engineering Virus-Based Nanoparticles for Biophotonics. Bioconjugate Chemistry, 2015, 26(1), 51–62. doi: 10.1021/bc500524f