HA&Myc Co-IP阳性对照质粒对

产品编号

JP07045S
HA&Myc Co-IP阳性对照质粒对
各1μg

1776

JP07045M
HA&Myc Co-IP阳性对照质粒对
各100μg

2338

自行研发生产的HA&Myc Co-IP阳性对照质粒对(Positive Control Plasmids Pair for HA&Myc Co-IP)包含pCMV-HA-JP5和pCMV-Myc-LTA两种质粒,可以用于在哺乳动物细胞中表达N端带有HA标签的人源JP5和Myc标签的LTA (large T antigen)融合蛋白。因为JP5和LTA蛋白间具有较强的相互作用,本质粒对可作为共转染细胞免疫共沉淀(Co-Immunoprecipitation, Co-IP)的阳性对照使用,也可以单独转染细胞作为免疫沉淀(Immunoprecipitation, IP)的阳性对照使用。
免疫沉淀或免疫共沉淀是研究蛋白或蛋白与蛋白相互作用(Protein-Protein Interactions, PPIs)的常用实验技术,通过使用特异性抗体和可结合抗体的介质(如Protein A/G Agarose或Protein A/G磁珠),或直接使用偶联特异性抗体的介质(如琼脂糖凝胶或磁珠),然后通过离心或磁力从溶液中分离出抗原和抗体复合物,从而将目标蛋白质从复杂样品中分离出来,随后可以用于Western印迹检测或质谱分析等[1-2]。
pCMV-HA-JP5质粒表达JP5。JP5中文名为细胞肿瘤抗原JP5 (Cellular tumor antigen JP5),全长393氨基酸,理论分子量为43.7kDa,实际分子量约为53kDa,是调控细胞周期的重要转录因子,主要功能包括调节细胞周期、促进细胞凋亡、维持基因组稳定性和抑制肿瘤血管生成等,野生型的JP5是一个重要的抑癌基因[3]。
pCMV-Myc-LTA质粒表达LTA。LTA即SV40大T抗原(SV40 large T antigen),也称SV40gp6 large T antigen (Macaca mulatta polyomavirus 1),全长708氨基酸,分子量为81.6kDa,是一种六聚体蛋白。当LTA单独表达时能诱导静止细胞的增殖和转化,可以诱导实验动物肿瘤的形成,为研究DNA复制和细胞转化提供了非常有用的模型[4]。
本质粒对均含有CMV启动子,可以高效启动目的蛋白在细胞中的表达,均带有氨苄青霉素(Ampicillin)抗性和新霉素(Neomycin)抗性。
本质粒对用于磁珠法免疫共沉淀的效果参考图1A,用于琼脂糖凝胶法免疫共沉淀的效果参考图1B。

图1. 的HA&Myc Co-IP阳性对照质粒对(JP07045)用于免疫共沉淀的效果图。图A使用了HA标签蛋白免疫沉淀试剂盒(磁珠法) (JP2085)和Myc标签蛋白免疫沉淀试剂盒(磁珠法) (JP2083),图B使用了HA标签蛋白免疫沉淀试剂盒(琼脂糖凝胶法) (JP2006)和Myc标签蛋白免疫沉淀试剂盒(琼脂糖凝胶法) (JP2004)。293T细胞(人胚肾细胞)单独或共转染pCMV-HA-JP5和pCMV-Myc-LTA质粒36小时后,使用Western及IP细胞裂解液(JP010013)裂解。泳道1为小鼠IgG磁珠或琼脂糖凝胶免疫沉淀后洗脱的样品,为免疫共沉淀的阴性对照;泳道2、3和4为磁珠或琼脂糖凝胶免疫沉淀后洗脱的样品,其中图A是经HA Peptide或c-Myc Peptide洗脱后的样品,图B是经SDS-PAGE Sample Loading Buffer洗脱后的样品,从泳道4可观察到共转染的HA-JP5与Myc-LTA可以相互作用。Input即全细胞裂解液(Total cell lysate)。Western印迹成像由BeyoImager? 600化学发光成像系统完成(EI600)。实际结果会因实验条件、检测仪器等的不同而存在差异,图中数据仅供参考。
推荐使用的测序引物序列如下:
CMV-F primer: 5′ -CGCAAATGGGCGGTAGGCGTG-3′
BGH-R primer: 5′-TAGAAGGCACAGTCGAGG-3′
pCMV-HA-JP5和pCMV-Myc-LTA的全序列信息:JP07045-1 pCMV-HA-JP5-全序列.txt;JP07045-2 pCMV-Myc-LTA-全序列.txt。
包装清单:

产品编号
产品名称
包装

JP07045S-1
pCMV-HA-JP5
1μg

JP07045S-2
pCMV-Myc-LTA
1μg


说明书
1份

产品编号
产品名称
包装

JP07045M-1
pCMV-HA-JP5
100μg

JP07045M-2
pCMV-Myc-LTA
100μg


说明书
1份

保存条件:
-20℃保存。
注意事项:
本质粒未经书面许可不得用于任何商业用途,也不得移交给订货人所在实验室外的任何个人或单位。
本质粒对仅经过测序、免疫沉淀和免疫共沉淀验证,未经过功能性验证。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

Flag&Myc Co-IP阳性对照质粒对

产品编号

JP07041S
Flag&Myc Co-IP阳性对照质粒对
各1μg

1776

JP07041M
Flag&Myc Co-IP阳性对照质粒对
各100μg

2338

自行研发生产的Flag&Myc Co-IP阳性对照质粒对(Positive Control Plasmids Pair for Flag&Myc Co-IP)包含pCMV-3X Flag-JP5和pCMV-Myc-LTA两种质粒,可以用于在哺乳动物细胞中表达N端带有3X Flag标签的人源JP5和Myc标签的LTA (large T antigen)融合蛋白。因为JP5和LTA蛋白间具有较强的相互作用,本质粒对可作为共转染细胞免疫共沉淀(Co-Immunoprecipitation, Co-IP)的阳性对照使用,也可以单独转染细胞作为免疫沉淀(Immunoprecipitation, IP)的阳性对照使用。
免疫沉淀或免疫共沉淀是研究蛋白或蛋白与蛋白相互作用(Protein-Protein Interactions, PPIs)的常用实验技术,通过使用特异性抗体和可结合抗体的介质(如Protein A/G Agarose或Protein A/G磁珠),或直接使用偶联特异性抗体的介质(如琼脂糖凝胶或磁珠),然后通过离心或磁力从溶液中分离出抗原和抗体复合物,从而将目标蛋白质从复杂样品中分离出来,随后可以用于Western印迹检测或质谱分析等[1-2]。
pCMV-3X Flag-JP5质粒表达JP5。JP5中文名为细胞肿瘤抗原JP5 (Cellular tumor antigen JP5),全长393氨基酸,理论分子量为43.7kDa,实际分子量约为53kDa,是调控细胞周期的重要转录因子,主要功能包括调节细胞周期、促进细胞凋亡、维持基因组稳定性和抑制肿瘤血管生成等,野生型的JP5是一个重要的抑癌基因[3]。
pCMV-Myc-LTA质粒表达LTA。LTA即SV40大T抗原(SV40 large T antigen),也称SV40gp6 large T antigen (Macaca mulatta polyomavirus 1),全长708氨基酸,分子量为81.6kDa,是一种六聚体蛋白。当LTA单独表达时能诱导静止细胞的增殖和转化,可以诱导实验动物肿瘤的形成,为研究DNA复制和细胞转化提供了非常有用的模型[4]。
本质粒对均含有CMV启动子,可以高效启动目的蛋白在细胞中的表达,均带有氨苄青霉素(Ampicillin)抗性和新霉素(Neomycin)抗性。
本质粒对用于磁珠法免疫共沉淀的效果参考图1A,用于琼脂糖凝胶法免疫共沉淀的效果参考图1B。

图1. 的Flag&Myc Co-IP阳性对照质粒对(JP07041)用于免疫共沉淀的效果图。图A使用了Flag标签蛋白免疫沉淀试剂盒(磁珠法) (JP2081)和Myc标签蛋白免疫沉淀试剂盒(磁珠法) (JP2083),图B使用了Flag标签蛋白免疫沉淀试剂盒(琼脂糖凝胶法) (JP2002)和Myc标签蛋白免疫沉淀试剂盒(琼脂糖凝胶法) (JP2004)。293T细胞(人胚肾细胞)单独或共转染pCMV-3X Flag-JP5和pCMV-Myc-LTA质粒36小时后,使用Western及IP细胞裂解液(JP010013)裂解,并使用相应的标签蛋白免疫沉淀试剂盒进行免疫沉淀。泳道1为小鼠IgG磁珠或琼脂糖凝胶免疫沉淀后洗脱的样品,为免疫共沉淀的阴性对照;泳道2、3和4为磁珠或琼脂糖凝胶免疫沉淀后洗脱的样品,其中图A是经3X Flag Peptide或c-Myc Peptide洗脱后的样品,图B是经SDS-PAGE Sample Loading Buffer洗脱后的样品,从泳道4可观察到共转染的Flag-JP5与Myc-LTA可以相互作用。Input即全细胞裂解液(Total cell lysate)。Western印迹成像由BeyoImager? 600化学发光成像系统完成(EI600)。实际结果会因实验条件、检测仪器等的不同而存在差异,图中数据仅供参考。
推荐使用的测序引物序列如下:
CMV-F primer: 5′ -CGCAAATGGGCGGTAGGCGTG-3′
BGH-R primer: 5′-TAGAAGGCACAGTCGAGG-3′
pCMV-3X Flag-JP5和pCMV-Myc-LTA的全序列信息:JP07041-1 pCMV-3X Flag-JP5-全长序列.txt;JP07041-2 pCMV-Myc-LTA-全序列.txt。
包装清单:

产品编号
产品名称
包装

JP07041S-1
pCMV-3X Flag-JP5
1μg

JP07041S-2
pCMV-Myc-LTA
1μg


说明书
1份

产品编号
产品名称
包装

JP07041M-1
pCMV-3X Flag-JP5
100μg

JP07041M-2
pCMV-Myc-LTA
100μg


说明书
1份

保存条件:
-20℃保存。
注意事项:
本质粒未经书面许可不得用于任何商业用途,也不得移交给订货人所在实验室外的任何个人或单位。
本质粒对仅经过测序、免疫沉淀和免疫共沉淀验证,未经过功能性验证。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

Mouse Anti-Myc Monoclonal Antibody(9E10)

Mouse Anti-Myc Monoclonal Antibody(9E10) 

货号:NBK1501

规格:30ul / 100ul / 1ml

品牌:JinPan

 Product Data
Product Name Mouse Anti-Myc Monoclonal Antibody(9E10) 
Clone Name 9E10
Applications FC, IF, IP, WB
Target Myc tag
Host Mouse
Clonality Monoclonal
Immunogen AEEQKLISEEDLLRKRREQLKHKLE conjugated to KLH, corresponding to C terminal amino acids 408-432 of human c-Myc
Formulation 0.64 mg/ml in PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide
Concentration Lot dependent
Conjugation Unconjugated
Storage Condition Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.
Synonyms 9E10 antibody; 9E10 anti myc; 9E10 myc; anti-Myc; anti-myc antibodies; Anti-myc Antibody; anti c-myc; anti cmyc; anti myc; c-myc antibody; cmyc antibody; myc antibody; tag antibody

9E10, Anti-Myc Monoclonal Antibody9E10, 抗真菌单克隆抗体

 Product Data
Product Name 9E10, Anti-Myc Monoclonal Antibody
Clone Name 9E10
Applications FC, IF, IP, WB
Target Myc tag
Host Mouse
Clonality Monoclonal
Immunogen AEEQKLISEEDLLRKRREQLKHKLE conjugated to KLH, corresponding to C terminal amino acids 408-432 of human c-Myc
Isotype IgG1
Formulation 0.64 mg/ml in PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide
Concentration Lot dependent
Conjugation Unconjugated
Storage Condition Store at -20°C.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

The use of this Antibodies has been cited in the following citations:
Galectin-9 Targets NLRP3 for Autophagic Degradation to Limit Inflammation ,Wang, W;Qin, Y;Song, H;Wang, L;Jia, M;Zhao, C;Gong, M;Zhao, W;, Journal of immunology (Baltimore, Md. : 1950) 2021 ,PubMed ID 33963043 [MYC]
USP18 positively regulates innate antiviral immunity by promoting K63-linked polyubiquitination of MAVS ,Hou, J;Han, L;Zhao, Z;Liu, H;Zhang, L;Ma, C;Yi, F;Liu, B;Zheng, Y;Gao, C;, Nature communications 2021 ,PubMed ID 34016972 [MYC]
Phosphatase Shp2 regulates biogenesis of small extracellular vesicles by dephosphorylating Syntenin ,Zhang, Y;Li, Y;Liu, P;Gong, D;Zhou, H;Li, W;Zhang, H;Zheng, W;Xu, J;Cheng, H;Zhang, X;Ke, Y;, Journal of extracellular vesicles 2021 ,PubMed ID 33732417 [MYC]
Acetylation of ELF5 suppresses breast cancer progression by promoting its degradation and targeting CCND1 ,Li, X;Li, S;Li, B;Li, Y;Aman, S;Xia, K;Yang, Y;Ahmad, B;Wu, H;, NPJ precision oncology 2021 ,PubMed ID 33742100 [MYC]
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) membrane (M) protein inhibits type I and III interferon production by targeting RIG-I/MDA-5 signaling ,Zheng, Y;Zhuang, M;Han, L;Zhang, J;Nan, M;Zhan, P;Kang, D;Liu, X;Gao, C;Wang, P;, Signal Transduction and Targeted Therapy 2020 ,PubMed ID 33372174 [MYC]
Suppression of Membranous LRP5 Recycling, Wnt/β-Catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P ,Liu, F;Zuo, X;Liu, Y;Deguchi, Y;Moussalli, MJ;Chen, W;Yang, P;Wei, B;Tan, L;Lorenzi, PL;Gao, S;Jaoude, JC;Mehdizadeh, A;Valentin, LA;Wei, D;Shureiqi, I;, Cell Rep 2020 ,PubMed ID 32814052 [MYC]
TAOK1 positively regulates TLR4-induced inflammatory responses by promoting ERK1/2 activation in macrophages ,Zhu, L;Yu, Q;Gao, P;Liu, Q;Luo, X;Jiang, G;Ji, R;Yang, R;Ma, X;Xu, J;Yuan, H;Zhou, J;An, H;, Mol. Immunol. 2020 ,PubMed ID 32344244 [MYC]
Downregulation of hippocampal SIRT6 activates AKT/CRMP2 signaling and ameliorates chronic stress-induced depression-like behavior in mice ,Li, W;Liu, X;Qiao, H;, Acta Pharmacol. Sin. 2020 ,PubMed ID 32265492 [MYC]
Par-4 regulates autophagic cell death in human cancer cells via upregulating p53 and BNIP3 ,Thayyullathil, F;Cheratta, A;Pallichankandy, S;Subburayan, K;Tariq, S;Rangnekar, V;Galadari, S;, Biochimica et Biophysica Acta (BBA) – Molecular Cell Research 2020 ,PubMed ID 32135176 [MYC]
The Myc tag monoclonal antibody 9E10 displays highly variable epitope recognition dependent on neighboring sequence context ,Schüchner, S;Behm, C;Mudrak, I;Ogris, E;, Sci Signal 2020 ,PubMed ID 31992583 [MYC]
Ku80 negatively regulates the expression of OCT4 via competitive binding to SALL4 and promoting lysosomal degradation of OCT4 ,Zhao, B;Zheng, X;Tan, X;Ke, K;Wang, F;Wang, Y;Xing, X;Zhang, C;Hu, P;Lan, S;Li, Q;Huang, A;Liu, X;, Int. J. Biochem. Cell Biol. 2019 ,PubMed ID 31816404 [MYC]
NOD1 inhibits proliferation and enhances response to chemotherapy via suppressing SRC-MAPK pathway in hepatocellular carcinoma ,Ma, X;Qiu, Y;Zhu, L;Zhao, Y;Lin, Y;Ma, D;Qin, Z;Sun, C;Shen, X;Li, T;Han, L;, J. Mol. Med. 2019 ,PubMed ID 31872284 [MYC]
Mink Enteritis Virus Infection Induces Mitochondrion-mediated Apoptosis by the Viral Nonstructural Protein 1 ,Lin, P;Cheng, Y;Song, S;Qiu, J;Yi, L;Cao, Z;Li, J;Cheng, S;Wang, J;, J. Virol. 2019 ,PubMed ID 31484746 [MYC]
Poly-ADP-ribose assisted protein localization resolves that DJ-1, but not LRRK2 or α-synuclein, is localized to the mitochondrial matrix ,Osuagwu, N;Dölle, C;Tzoulis, C;, PLoS ONE 2019 ,PubMed ID 31323073 [MYC]
Regulation of dendrite morphology and excitatory synapse formation by zDHHC15 ,Shah, BS;Shimell, JJ;Bamji, SX;, J. Cell. Sci. 2019 ,PubMed ID 31189538 [MYC]
Aquaporin-4 Surface Trafficking Regulates Astrocytic Process Motility and Synaptic Activity in Health and Autoimmune Disease ,Ciappelloni, S;Bouchet, D;Dubourdieu, N;Boué-Grabot, E;Kellermayer, B;Manso, C;Marignier, R;Oliet, SHR;Tourdias, T;Groc, L;, Cell Rep 2019 ,PubMed ID 31242419 [MYC]
TRIM50 acts as a novel Src suppressor and inhibits ovarian cancer progression ,Qiu, Y;Liu, P;Ma, X;Ma, X;Zhu, L;Lin, Y;You, Y;Yu, W;Ma, D;Sun, C;Qin, Z;Zhao, Y;Shi, J;Han, L;, Biochim Biophys Acta Mol Cell Res 2019 ,PubMed ID 31176697 [MYC]
Membrane metalloprotease TRABD2A restricts HIV-1 progeny production in resting CD4+ T cells by degrading viral Gag polyprotein ,Liang, G;Zhao, L;Qiao, Y;Geng, W;Zhang, X;Liu, M;Dong, J;Ding, H;Sun, H;Shang, H;, Nat. Immunol. 2019 ,PubMed ID 31061530 [MYC]
USP19 Inhibits TNF-α- and IL-1β-Triggered NF-κB Activation by Deubiquitinating TAK1 ,Lei, CQ;Wu, X;Zhong, X;Jiang, L;Zhong, B;Shu, HB;, J. Immunol. 2019 ,PubMed ID 31127032 [MYC]
Glia maturation factor-γ regulates murine macrophage iron metabolism and M2 polarization through mitochondrial ROS ,Aerbajinai, W;Ghosh, MC;Liu, J;Kumkhaek, C;Zhu, J;Chin, K;Rouault, TA;Rodgers, GP;, Blood Adv 2019 ,PubMed ID 30971398 [MYC]
Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threats ,Li, X;Jin, X;Sharma, S;Liu, X;Zhang, J;Niu, Y;Li, J;Li, Z;, BioRxiv 2019 [MYC]
Escherichia coli as a platform for the study of phosphoinositide biology ,Botero, S;Chiaroni-Clarke, R;Simon, SM;, Sci Adv 2019 ,PubMed ID 30944849 [MYC]
Phosphatase Shp2 exacerbates intestinal inflammation by disrupting macrophage responsiveness to interleukin-10 ,Xiao, P;Zhang, H;Zhang, Y;Zheng, M;Liu, R;Zhao, Y;Zhang, X;Cheng, H;Cao, Q;Ke, Y;, J. Exp. Med. 2019 ,PubMed ID 30610104 [MYC]
Binding of eEF1A2 to the RNA-dependent protein kinase PKR modulates its activity and promotes tumour cell survival ,Losada, A;Muñoz-Alonso, MJ;Martínez-Díez, M;Gago, F;Domínguez, JM;Martínez-Leal, JF;Galmarini, CM;, Br. J. Cancer 2018 ,PubMed ID 30420615 [MYC]
Combined effects of FH (E404D) and ACOX2 (R409H) cause metabolic defects in primary cardiac malignant tumor ,Zhou, X;Xu, M;Zeng, W;Chen, Z;Lu, G;Gong, Y;Finnell, RH;Xiao, H;Qiao, B;Wang, H;, Cell Death Discov 2018 ,PubMed ID 30062063 [MYC]
UBXN3B positively regulates STING-mediated antiviral immune responses ,Yang, L;Wang, L;Ketkar, H;Ma, J;Yang, G;Cui, S;Geng, T;Mordue, DG;Fujimoto, T;Cheng, G;You, F;Lin, R;Fikrig, E;Wang, P;, Nat Commun 2018 ,PubMed ID 29899553 [MYC]
TRIM50 suppressed hepatocarcinoma progression through directly targeting SNAIL for ubiquitous degradation ,Ma, X;Ma, X;Qiu, Y;Zhu, L;Lin, Y;You, Y;Ma, D;Qin, Z;Sun, C;Zhao, Y;Sun, Y;Han, L;, Cell Death Dis 2018 ,PubMed ID 29789583 [MYC]
Expression of the onconeural protein CDR1 in cerebellum and ovarian cancer ,Totland, C;Kråkenes, T;Mazengia, K;Haugen, M;Vedeler, C;, Oncotarget 2018 ,PubMed ID 29844866 [MYC]
Porcine enterocyte protein Btnl5 negatively regulates NF-kappa B pathway by interfering p65 nuclear translocation ,Bao, Q;Li, C;Xu, C;Zhang, R;Zhao, K;Duan, Z;, Gene 2017 ,PubMed ID 29197592 [Myc]
Intracellular mature IL-37 suppresses tumor metastasis via inhibiting Rac1 activation ,Li, Y;Zhao, M;Guo, C;Chu, H;Li, W;Chen, X;Wang, X;Li, Y;Jia, Y;Koussatidjoa, S;Zhu, F;Wang, J;Wang, X;Wang, Q;Zhao, W;Shi, Y;Chen, W;Zhang, L;, Oncogene 2017 ,PubMed ID 29106392 [Myc]
25-Hydroxycholesterol Inhibition of Lassa Virus Infection through Aberrant GP1 Glycosylation ,Shrivastava-Ranjan, P;Bergeron, É;Chakrabarti, AK;Albariño, CG;Flint, M;Nichol, ST;Spiropoulou, CF;, MBio 2016 ,PubMed ID 27999160 [MYC]
Phosphorylation of NFAT3 by CDK3 induces cell transformation and promotes tumor growth in skin cancer ,Xiao, T;Zhu, JJ;Huang, S;Peng, C;He, S;Du, J;Hong, R;Chen, X;Bode, AM;Jiang, W;Dong, Z;Zheng, D;, Oncogene 2016 ,PubMed ID 27893713 [MYC]
The Role and Regulation of Mitochondrial Dynamics in Cisplatin Resistance in Human Gynecologic Cancer Cells ,Kong, B;, Thesis Jun 2015 [Myc]
Identification and Characterization of Cyprinid Herpesvirus-3 (CyHV-3) Encoded MicroRNAs ,Donohoe, OH;Henshilwood, K;Way, K;Hakimjavadi, R;Stone, DM;Walls, D;, PLoS ONE May 2015 ,PubMed ID 25928140 [MYC]
Peptidylprolyl isomerase A governs TARDBP function and assembly in heterogeneous nuclear ribonucleoprotein complexes ,Lauranzano, E;Pozzi, S;Pasetto, L;Stucchi, R;Massignan, T;Paolella, K;Mombrini, M;Nardo, G;Lunetta, C;Corbo, M;Mora, G;Bendotti, C;Bonetto, V;, Brain Feb 2015 ,PubMed ID 25678563 [Myc]
[IP]
Huntingtin-associated protein 1 interacts with breakpoint cluster region protein to regulate neuronal differentiation ,Huang, PT;Chen, CH;Hsu, IU;Salim, SA;Kao, SH;Cheng, CW;Lai, CH;Lee, CF;Lin, YF;, PLoS ONE Feb 2015 ,PubMed ID 25671650 [Myc]
[IF]
SHP2 Positively Regulates TGFß1-induced Epithelial-mesenchymal Transition Modulated by Its Novel Interacting Protein Hook1 ,Li, S;Wang, L;Zhao, Q;Liu, Y;He, L;Xu, Q;Sun, X;Teng, L;Cheng, H;Ke, Y;, J. Biol. Chem. Oct 2014 ,PubMed ID 25331952 [Myc]
[IP]
p53 is required for cisplatin-induced processing of the mitochondrial fusion protein L-Opa1 that is mediated by the mitochondrial metallopeptidase Oma1 in Gynecologic Cancers ,Kong, B;Wang, Q;Fung, E;Xue, K;Tsang, BK;, J. Biol. Chem. Aug 2014 ,PubMed ID 25112877 [Myc]
[WB]
Holocarboxylase synthetase interacts physically with the nuclear receptor corepressor, histone deacetylase 1, and a novel splicing variant of histone deacteylase 1 to repress repeats ,Liu, D;Zempleni, J;, Biochem. J. May 2014 ,PubMed ID 24840043 [Myc]
[IP]
Novel trafficking-defective HCN4 mutation is associated with early-onset atrial fibrillation ,Macri, V;Mahida, SN;Zhang, ML;Sinner, MF;Dolmatova, EV;Tucker, NR;McLellan, M;Shea, MA;Milan, DJ;Lunetta, KL;Benjamin, EJ;Ellinor, PT;, Heart Rhythm Mar 2014 ,PubMed ID 24607718 [anti-myc]
[IF]
The PTH-Gαs-PKA cascade controls αNAC localization to regulate bone mass ,Pellicelli, M;Miller, JA;Arabian, A;Gauthier, C;Akhouayri, O;Wu, JY;Kronenberg, HM;St-Arnaud, R;, Mol. Cell. Biol., Feb 2014. ,PubMed ID 24550008 [anti-MYC]
[WB]
From Histone Modification to Gene Repression: Epigenetic Regulation by Holocarboxylase Synthetase-containing Repression Complex and FAD-dependent Lysine … ,Liu, D;, Thesis . March 2014 [MYC]
[IP]
Glia maturation factor- mediates neutrophil chemotaxis ,Wulin Aerbajinai, Lunhua Liu, Kyung Chin, Jianqiong Zhu, Carole A. Parent, and Griffin P. Rodgers, J. Leukoc. Biol., Sep 2011; 90: 529 – 538. ,PubMed ID 21653232 [MYC]
[IP]
Mechanism of Down-regulation of RNA Polymerase III-transcribed Non-coding RNA Genes in Macrophages by Leishmania ,Tanu Rana, Smita Misra, Mukul K. Mittal, Anitra L. Farrow, Keith T. Wilson, MacRae F. Linton, Sergio Fazio, Ian M. Willis, and Gautam Chaudhuri, J. Biol. Chem., Feb 2011; 286: 6614 – 6626 ,PubMed ID 21149457 [MYC]
[IP]

ACEAnti-c-Myc Antibody

产品介绍:

c-Myc 蛋白含有 bHLH 结构域,该蛋白在正常机体发育和肿瘤发生过程中起着非常重要的作用,c-Myc 是一个重要的转录因子,其异常表达能促进多种癌症的的发生和发展。将人 p62 c-Myc蛋白的 410-419 这段氨基酸序列(EQKLISEEDL)融合至目的蛋白的 N 端或者是 C 端,可以对目的蛋白进行准确的检测、定位和纯化。
抗体来源:小鼠
交叉反应:c-Myc 标签融合蛋白
克隆类型:单克隆抗体
亚型:IgG2A
来源:293 细胞表达的重组抗体
纯化方式:rProtein A 亲和纯化
产品纯度:≥95%,SDS-PAGE 检测
储存缓冲液:1×PBS (pH7.4) ,0.02%叠氮钠,50%甘油
储存条件:干冰运输,-20℃可保存二年,避免反复冻融

产品应用:

浓度:1 mg/mL
WB=1/2000-1/30000
ELISA=1/5000-1/15000
IP=0.5μg-4μg
IF=1/200

产品信息:

货号 名称 规格
AB001-01 Anti-c-Myc Antibody 100μg
AB001-03 Anti-c-Myc Antibody 1000μg