Nugen 0340-32 人类FFPE RNA文库构建试剂盒在Illumina仪器平台上测序的链特异性RNA-Seq文库构建提供了整体解决方案

Ovation人类FFPE RNA-Seq多重文库构建试剂盒是为在Illumina仪器平台上测序的链特异性RNA-Seq文库构建提供了整体解决方案。 该试剂盒结合了高效灵活的工作流程,适用于大范围的总RNA输入(10-250 ng),并采用AnyDeplete技术有效去除人类rRNA序列的垃圾背景序列。

Ovation人类FFPE RNA-Seq多重文库构建试剂盒使用从人类组织或细胞系获得的纳克级总RNA,为链特异性RNA-Seq文库构建提供了端到端的解决方案。该工作流程同样适用于新鲜冷冻样品(低至10 ng)的总RNA或FFPE样品(低至100 ng)的保存RNA。从总RNA输入到完整构建的文库的整合工作流程具有很高的可重复性,可以在大约7小时内完成。 NuGEN的依赖插入的AnyDeplete技术可有针对性地消除不需要的转录本,并显着减少了源自人类rRNA(12S,16S,18S和28S基因)的测序数据,从而更有效地利用了测序通量。该试剂盒均提供八个*的条形码接头,以使多重测序能够进一步优化功效。

简化的工作流程包括使用随机和多聚(T)引物的混合物反转录生成双链cDNA,双链cDNA打断,末端修复以生成平末端,接头进行连接,通过核苷酸类似物靶向降解的链选择,AnyDeplete技术介导的接头裂解和PCR扩增以产生终文库。 整个工作流程,包括片段化,都可在短短7个小时内完成,并生成可用于在Illumina测序平台上进行单端或双端测序的簇形成的DNA文库。

NuGEN系列产品

NuGEN #0360-08 Celero DNA-Seq , 8rxn
NuGEN #0360-24 Celero DNA-Seq , 24rxn
NuGEN #0357-08 Trio RNA-Seq , 8rxn
NuGEN #0357-32 Trio RNA-Seq , 32rxn
NuGEN #0357-96 Trio RNA-Seq , 96rxn
NuGEN #0508-08 Universal Plus mRNA-Seq , 8rxn
NuGEN #0508-32 Universal Plus mRNA-Seq , 32rxn
NuGEN #0508-96 Universal Plus mRNA-Seq , 96rxn
NuGEN #0508-A01 Universal Plus mRNA-Seq , 96rxn
NuGEN #0520-24 Universal Plus mRNA-Seq with NuQuant , 24rxn
NuGEN #0520-A01 Universal Plus mRNA-Seq with NuQuant , 96rxn
NuGEN #0500-32 Ovation® SoLo RNA-Seq System, Human , 32rxn
NuGEN #0500-96 Ovation® SoLo RNA-Seq System, Human , 96rxn
NuGEN #0501-32 Ovation® SoLo RNA-Seq System, Mouse , 32rxn
NuGEN #0501-96 Ovation® SoLo RNA-Seq System, Mouse , 96rxn
NuGEN #7102-08 Ovation® RNA-Seq System V2 , 8rxn
NuGEN #7102-32 Ovation® RNA-Seq System V2 , 32rxn
NuGEN #7102-A01 Ovation® RNA-Seq System V2 , 96rxn
NuGEN #7150-08 Ovation® RNA-Seq FFPE System , 8rxn
NuGEN #0335-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 1-8 , 32rxn
NuGEN #0336-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 9-16 , 32rxn
NuGEN #9113-A01 Ovation® Ultralow Methyl-Seq System , 96rxn
NuGEN #0535-32 Ovation Ultralow Methy-Seq DR Multiplex System 1-8, with TrueMethyl® oxBS , 32rxn
NuGEN #0536-32 Ovation Ultralow Methy-Seq DR Multiplex System 9-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0553-32 Ovation RRBS Methyl-Seq System 1-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0414-32 TrueMethyl® oxBS Module , 32rxn
NuGEN #3302-12 Ovation® Pico WTA System V2 , 12rxn
NuGEN #3302-60 Ovation® Pico WTA System V2 , 60rxn
NuGEN #3302-A01 Ovation® Pico WTA System V2 , 96rxn
NuGEN #3403-12 Ovation® FFPE WTA System , 12rxn
NuGEN #3403-60 Ovation® FFPE WTA System , 60rxn
NuGEN #3403-A01 Ovation® FFPE WTA System , 96rxn
NuGEN #3100-12 Ovation® RNA Amplification System V2 , 12rxn
NuGEN #3100-60 Ovation® RNA Amplification System V2 , 60rxn
NuGEN #3100-A01 Ovation® RNA Amplification System V2 , 96rxn
NuGEN #0344-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-08 Ovation® Ultralow System V2 , 8rxn
NuGEN #0344NB-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-A01 Ovation® Ultralow System V2 , 96rxn

NuGEN 0506 Trio微量样本全转录组文库制备试剂盒

Trio RNA-Seq文库制备试剂盒提供同类更佳的NGS文库制备系统和RNA测序解决方案,用于液体活检,FFPE和其他微量的挑战性样品。

Trio微量RNA-Seq专为稳定可靠的RNA-Seq文库制备而设计,可与500 pg-50 ng的总RNA配合使用,可帮助研究人员从几乎任何样本中捕获完整的生物学信息。

更新的Trio微量RNA-Seq文库制备试剂盒现在集成了来自大量文献发表的Ovation®RNA-Seq System V2和双重index的成熟技术,可提供从低样本量或低频转录本的总RNA到完整文库制备的准确流程。

NuGEN的双重index可以方便地以预装微孔板96个的条形码对,因此无需手动准备条形码对。只需将接头添加到样品中并连接,每个样品将具有index1和index2条形码,这些条形码与平板中的所有其他样品不同。

Trio微量RNA-Seq文库制备结合了三种强大的技术:

1.单引物等温扩增技术(SPIA)可以扩增微量的和降解的样品。

2. 酶切片段化和DimerFree文库构建激素允许高效和稳定的文库制备。

3.在构建文库后,AnyDeplete技术可定制转录本耗尽方案,大化信息序列reads全转录组数据。

这种的工作流程为整个转录组文库提供了无接头二聚体和整合的无用转录本耗尽,从而增强了检测和更均匀的转录本覆盖率。

NuGEN系列产品

NuGEN #0360-08 Celero DNA-Seq , 8rxn
NuGEN #0360-24 Celero DNA-Seq , 24rxn
NuGEN #0357-08 Trio RNA-Seq , 8rxn
NuGEN #0357-32 Trio RNA-Seq , 32rxn
NuGEN #0357-96 Trio RNA-Seq , 96rxn
NuGEN #0508-08 Universal Plus mRNA-Seq , 8rxn
NuGEN #0508-32 Universal Plus mRNA-Seq , 32rxn
NuGEN #0508-96 Universal Plus mRNA-Seq , 96rxn
NuGEN #0508-A01 Universal Plus mRNA-Seq , 96rxn
NuGEN #0520-24 Universal Plus mRNA-Seq with NuQuant , 24rxn
NuGEN #0520-A01 Universal Plus mRNA-Seq with NuQuant , 96rxn
NuGEN #0500-32 Ovation® SoLo RNA-Seq System, Human , 32rxn
NuGEN #0500-96 Ovation® SoLo RNA-Seq System, Human , 96rxn
NuGEN #0501-32 Ovation® SoLo RNA-Seq System, Mouse , 32rxn
NuGEN #0501-96 Ovation® SoLo RNA-Seq System, Mouse , 96rxn
NuGEN #7102-08 Ovation® RNA-Seq System V2 , 8rxn
NuGEN #7102-32 Ovation® RNA-Seq System V2 , 32rxn
NuGEN #7102-A01 Ovation® RNA-Seq System V2 , 96rxn
NuGEN #7150-08 Ovation® RNA-Seq FFPE System , 8rxn
NuGEN #0335-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 1-8 , 32rxn
NuGEN #0336-32 Ovation® Ultralow Methyl-Seq DR Multiplex System 9-16 , 32rxn
NuGEN #9113-A01 Ovation® Ultralow Methyl-Seq System , 96rxn
NuGEN #0535-32 Ovation Ultralow Methy-Seq DR Multiplex System 1-8, with TrueMethyl® oxBS , 32rxn
NuGEN #0536-32 Ovation Ultralow Methy-Seq DR Multiplex System 9-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0553-32 Ovation RRBS Methyl-Seq System 1-16, with TrueMethyl® oxBS , 32rxn
NuGEN #0414-32 TrueMethyl® oxBS Module , 32rxn
NuGEN #3302-12 Ovation® Pico WTA System V2 , 12rxn
NuGEN #3302-60 Ovation® Pico WTA System V2 , 60rxn
NuGEN #3302-A01 Ovation® Pico WTA System V2 , 96rxn
NuGEN #3403-12 Ovation® FFPE WTA System , 12rxn
NuGEN #3403-60 Ovation® FFPE WTA System , 60rxn
NuGEN #3403-A01 Ovation® FFPE WTA System , 96rxn
NuGEN #3100-12 Ovation® RNA Amplification System V2 , 12rxn
NuGEN #3100-60 Ovation® RNA Amplification System V2 , 60rxn
NuGEN #3100-A01 Ovation® RNA Amplification System V2 , 96rxn
NuGEN #0344-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-08 Ovation® Ultralow System V2 , 8rxn
NuGEN #0344NB-32 Ovation® Ultralow System V2 , 32rxn
NuGEN #0344NB-A01 Ovation® Ultralow System V2 , 96rxn