标签归档：polymerase

Lucigen | LGC代理,Exo-Minus Klenow DNA Polymerase (D355A, E357A),部分现货

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Exo-Minus Klenow DNA Polymerase (D355A, E357A)

A Klenow DNA polymerase lacking all 5' to 3' and 3' to 5' exonuclease activity.

Product Details

The Exo-Minus Klenow DNA Polymerase (D355A, E357A) Kits include Exo-Minus Klenow DNA Polymerase (D355A, E357A) at 10 U/µL and 10X Reaction Buffer.

The “enzyme only” format contains Exo-Minus Klenow DNA Polymerase (D355A, E357A) at 10 U/µL.

Key features

  • A Klenow DNA polymerase lacking all 5' to 3' and 3' to 5' exonuclease activity
  • Synthesise DNA from ssDNA templates without degrading any single-stranded DNA
  • Suitable for a variety of applications including random-primer labelling of DNA,1second-strand cDNA synthesis,2sStrand-displacement amplification3

Lucigen | LGC, Biosearch Technologies主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、聚合酶及E.cloni®感受态细胞及蛋白表达系统等。

Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。此外，Lucigen还是全球科学家青睐的Epicentre品牌产品的供应商。在2018年，Lucigen被LGC收购，从而加强了其在基因组市场的试剂供应。Lucigen的产品和服务已经覆盖了临床诊断、药物开发、合成生物学和基因编辑等多个领域。更多产品信息欢迎访问Lucigen | LGC, Biosearch Technologies官网。

Lucigen | LGC代理,NxGen phi29 DNA Polymerase,部分现货

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NxGen phi29 DNA Polymerase

NxGen Phi29 DNA Polymerase is a highly processive and high-fidelity DNA Polymerase that enables accurate amplification of DNA from limited or degraded samples. 

Product Details

The NxGen Phi29 DNA Polymerase sources its enzyme from a recombinant E. coli strain carrying the phi29 DNA polymerase gene from bacteriophage phi29.

1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30 °C.

The enzyme storage buffer consists of 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.5% Tween-20, 0.5% NP-40, 50% glycerol, pH 7.4 @ 25 °C and the 10X phi29 DNA Polymerase Buffer is composed of 500 mM Tris-HCl, 100 mM (NH4)2SO4, 40 mM Dithiothreitol, 100 mM MgCl2, pH 7.5 @ 25 °C.

Key features

  • Powerful strand displacement activity, making it ideal for challenging samples or damaged DNA

  • Highly processive, allowing for amplification of up to 70,000 base insertions per binding event

  • High-fidelity 3'->5' proofreading exonuclease function for accurate DNA replication with low error rates

Lucigen | LGC, Biosearch Technologies主要开发各类用于基因克隆的试剂盒及相关产品，包括：CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、聚合酶及E.cloni®感受态细胞及蛋白表达系统等。

Lucigen公司凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。此外，Lucigen还是全球科学家青睐的Epicentre品牌产品的供应商。在2018年，Lucigen被LGC收购，从而加强了其在基因组市场的试剂供应。Lucigen的产品和服务已经覆盖了临床诊断、药物开发、合成生物学和基因编辑等多个领域。更多产品信息欢迎访问Lucigen | LGC, Biosearch Technologies官网。