> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物素标记率

0.7-1 as determined by the HABA assay.

生物活性

Immobilized human ACE2 protein (Fc tag) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2(BA.4/BA.5/BA.5.2)Spike RBD Protein(His&AVI Tag),Biotinylated , the EC50 is 15-45 ng/mL.

蛋白构建

A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutation G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H) (Arg319-Phe541) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.4/BA.5/BA.5.2).

表达宿主

HEK293 Cells

种属

SARS-CoV-2

预测 N 端

Arg 319

分子量

The recombinant SARS-CoV-2 (BA.4/BA.5/BA.5.2) Spike RBD consists of 249 amino acids and predicts a molecular mass of 28.49 kDa. As a result of glycosylation, it migrates as an approximately 38.44 kDa band in SDS-PAGE under reducing conditions.

缓冲液

Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶