标签归档：recombinant

OrganRegen-Recombinant Human Noggin（807-NOG）

发表于由

Recombinant Human Noggin

货号：807-NOG

规格：1mg

100ug

品牌：OrganRegen

产品介绍

Background:

Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs) [1] . Secreted Noggin probably remains close to the cell surface due to its binding of heparin‑containing proteoglycans. Noggin binds some BMPs such as BMP-4 with high affinity and others such as BMP-7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors [2]. During embryogenesis, Noggin antagonizes specific BMPs at defined times, for example, during neural tube, somite and cardiomyocyte growth and patterning. During skeletal development, Noggin prevents chondrocyte hyperplasia, thus allowing proper formation of joints [2] . Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin [3] . During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons [4].

 

技术参数

Source:

Chinese Hamster Ovary cell line

Protein Construction:

A DNA sequence encoding the amino acids (Gln28-Cys232) of human Noggin (Accession Number: Q13253) was expressed.

Synonyms:

NOG; SYM1; symphalangism 1 (proximal); synostoses (multiple) syndrome 1; SYNS1; SYNS1A Purity:

≥ 95%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.

Biological Activity:

Measured by its ability to inhibit recombinant human BMP4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is typically 0.05-0.3 μg/ml in the presence of 50 ng/ml of recombinant human BMP4.

Endotoxin Level:

<0.10 EU per 1 μg of the protein by the LAL method

Calculated Molecular Weight:

25.1 kDa

SDS-PAGE:

30-36 kDa, reducing conditions OrganRegen-Recombinant Human Noggin（807-NOG）

FORMULATION AND STORAGE

Formulation:

The product is Lyophilized from a 0.22 μm filtered solution in PBS.

Shipping:

The product is shipped on ice. Upon receipt, store it immediately at as methodsrecommended below.

Reconstitution:

Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Stability & Storage:

24 months, -20 to -70 °C, under powder state;

12 months, -20 to -70 °C, under sterile conditions after reconstitution;

2 month, 2 to 8 °C under sterile conditions after reconstitution; avoid repeated freeze-thaw cycles.

References:

  1. J, G., et al., Structural basis of BMP signalling inhibition by the cystine knot protein Noggin. Nature, 2002. 420(6916): p. 636-42.

  2. Brunet, L.J., et al., Noggin, cartilage morphogenesis, and joint formation in the mammalian skeleton. Science, 1998. 280(5368): p. 1455-7.

  3. Bonaguidi, M.A., et al., Noggin expands neural stem cells in the adult hippocampus. J Neurosci, 2008. 28(37): p. 9194-204.

  4. Xu, R.H., et al., Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Nat Methods, 2005. 2(3): p. 185-90.

OrganRegen-Recombinant Human FGF10（816-FGF）

发表于由

Recombinant Human FGF10

货号：816-FGF

规格：1mg

100ug

品牌：OrganRegen

产品介绍

Background:

FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP. It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) [1]. The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung [2, 3]. Overlapping expression patterns and activities with FGF-3, -7, and -8 suggest at least a partial redundancy in FGF‑10 biology. FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer [4] .

 

技术参数

Source:

E.coli

Protein Construction:

A DNA sequence encoding the amino acids (Gln38-Ser208) of human FGF10 (Accession Number: O15520) was expressed.

Synonyms:

Fibroblast growth factor 10; Keratinocyte growth factor 2; KGF2; KGF-2. Purity:

≥ 95%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.

Biological Activity:

Measured in a cell proliferation assay using human liver ductal organoids. The ED50 for this effect is 20-100 ng/ml.

Endotoxin Level:

<0.10 EU per 1 μg of the protein by the LAL method

Calculated Molecular Weight:

19.5 kDa

SDS-PAGE:

25 kDa, reducing condition

OrganRegen-Recombinant Human FGF10（816-FGF）

Formulation:

The product is Lyophilized from a 0.22μm filtered solution in PBS.

Shipping:

The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.

Reconstitution:

Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Stability & Storage:

24 months, -20 to -70 °C, under powder state; 12 months, -20 to -70 °C, under sterile conditions after reconstitution; 2 month, 2 to 8 °C under sterile conditions after reconstitution; avoid repeated freeze-thaw cycles.

References:

  1. Zhang, X., et al., Receptor specificity of the fibroblast growth factor family. The complete mammalian FGF family. J Biol Chem, 2006. 281(23): p. 15694-700.

  2. Bellusci, S., et al., Fibroblast growth factor 10 (FGF10) and branching morphogenesis in the embryonic mouse lung. Development, 1997. 124(23): p. 4867-78.

  3. Weaver, M., N.R. Dunn, and B.L. Hogan, Bmp4 and Fgf10 play opposing roles during lung bud morphogenesis. Development, 2000. 127(12): p. 2695-704.

  4. Nomura, S., et al., FGF10/FGFR2 signal induces cell migration and invasion in pancreatic cancer. Br J Cancer, 2008. 99(2): p. 305-13.

OrganRegen-Recombinant Wnt β-Catenin Ligand（RWL003）

发表于由

Recombinant Wnt/β-Catenin Ligand

货号：RWL003

规格：1mg

100ug

10ug

品牌：OrganRegen

产品介绍

DESCRIPTION

Background:

The Wnt signal transduction is essential for cell proliferation and differentiation and the self-renewal of stem cells. Wnt ligands heterodimerize Frizzled (Fz) family receptors and their co-receptors Lrp5/6, triggering downstream signaling pathways including the canonical β-catenin cascade, and resulting in expression of genes regulating cell fate determination. is a 59.6kDa protein that dimerizes Fz and Lrp5/6 and activates canonical Wnt/β-Catenin signaling. Recombinant Wnt/β-Catenin Ligand potently supports different types of organoid growth, including hepatocyte, cholangiocyte, alveoli, breast, colon, pancreas, esophagus and ovarian.

 

技术参数

Source:

Chinese Hamster Ovary cell line

Protein Construction:

Recombinant Wnt/β-Catenin Ligand is a Fc-tagged recombinant protein that dimerizes Fz and Lrp5/6 and activates canonical Wnt/β-Catenin signaling. Protein Construction is confidential.

SPECIFICATIONS

Purity:

≥ 98%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.

Biological Activity:

Measured by its ability to induce TOPflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is <50ng/mL. Measured its activity in organoid expansion assay using human liver ductal organoids.

Endotoxin Level:

<0.10 EU per 1 μg of the protein by the LAL method

Calculated Molecular Weight:

59.6 kDa

SDS-PAGE:

64.0 kDa, reducing condition

DATA

OrganRegen-Recombinant Wnt β-Catenin Ligand（RWL003）

FORMULATION AND STORAGE

Formulation:

The product is Lyophilized from a 0.25 μm filtered solution in PBS.

Shipping:

The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.

Reconstitution:

Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Stability & Storage:

24 months, -20 to -70 °C, under powder state;

12 months, -20 to -70 °C, under sterile conditions after reconstitution;

2 month, 2 to 8 °C under sterile conditions after reconstitution;

avoid repeated freeze-thaw cycles.

OrganRegen-Recombinant Human FGF2（913-FG2）

发表于由

Recombinant Human FGF2

货号：913-FG2

规格：1mg

100ug

品牌：OrganRegen

产品介绍

Background:

Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the FGF superfamily of mitogenic proteins which show 35-60% amino acid conservation[1]. FGF acidic and basic are unique from other members of the family in that they lack classical secretory signal peptides. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury[2]. FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may also participate in the development of several pathological conditions resulting from excessive cell proliferation and/or angiogenesis[3]. BFGF is a critical component of human embryonic stem cell culture medium[4].

技术参数

Source:

E.coli

Protein Construction:

A DNA sequence encoding the amino acids (Pro143-Ser288) of human FGF-2 (Accession Number:

P48798) was expressed.

Synonyms:

Basic fibroblast growth factor; bFGF; FGF basic; FGF-2.

SPECIFICATIONS

Purity:

≥ 97%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.

Biological Activity:

Measured in CellTiter-Glo 3D Cell Viability Assay using human bronchoalveolar organoids. The ED50 for this effect is 10-50 ng/ml.

Endotoxin Level:

<0.10 EU per 1 μg of the protein by the LAL method

Calculated Molecular Weight:

16.5 kDa

SDS-PAGE:

18 kDa, reducing conditions

DATA

OrganRegen-Recombinant Human FGF2（913-FG2）

 

 

FORMULATION AND STORAGE

Formulation:

The product is Lyophilized from a 0.22 μm filtered solution in PBS.

Shipping:

The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.

Reconstitution:

Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Stability & Storage:

24 months, -20 to -70 °C, under powder state;

12 months, -20 to -70 °C, under sterile conditions after reconstitution;

2 month, 2 to 8 °C under sterile conditions after reconstitution;

avoid repeated freeze-thaw cycles.

                                                                                                                          

References:

1.          Takayama, S., et al., Periodontal regeneration by FGF-2 (bFGF) in primate models. Journal of dental research, 2001. 80(12): p. 2075-9.

2.          Penaultllorca, F., et al., Expression of variant forms of fibroblast growth factor receptor 2 mRNA in human breast carcinomas. International journal of oncology, 1997. 10(6): p. 1191-6.

3.          Niu, Y., et al., Therapeutic effect of bFGF on retina ischemia-reperfusion injury. Chinese medical journal, 2004. 117(2): p. 252-7.

4.          Zhang, Y., et al., Expression of aFGF, bFGF, and FGFR1 in ovarian epithelial neoplasm. Chinese medical journal, 2004. 117(4): p. 601-3.

OrganRegen-Recombinant Human FGF7(923-FG7)

发表于由

‍Recombinant Human FGF7

货号：923-FG7

规格：1mg

100ug

品牌：OrganRegen‍

产品介绍

DESCRIPTION

Background:

FGF-7 (fibroblast growth factor-7) is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis[1].FGF-7 promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation[2]. It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies. Deletion of FGF-7 affects kidney development, producing abnormally small ureteric buds and fewer nephrons[3] . It also impedes hair follicle differentiation [4]. The 194 amino acid (aa) FGF-7 precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites.

技术参数

Source:

Chinese Hamster Ovary cell line

Protein Construction:

A DNA sequence encoding the amino acids (Cys32-Thr194) of human FGF-7 (Accession Number:

P21781) was expressed.

Synonyms:

HBGF-7; Heparin-binding growth factor 7; keratinocyte growth factor; KGF.

SPECIFICATIONS

Purity:

≥ 95%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.

Biological Activity:

Measured in CellTiter-Glo 3D Cell Viability Assay using human bronchoalveolar organoids. The ED50 for this effect is 5-10 ng/ml.

Endotoxin Level:

<0.10 EU per 1 μg of the protein by the LAL method

Calculated Molecular Weight:

19 kDa

SDS-PAGE:

22 kDa, reducing conditions 

DATA

 

OrganRegen-Recombinant Human FGF7(923-FG7)

 

FORMULATION AND STORAGE

Formulation:

The product is Lyophilized from a 0.22 μm filtered solution in PBS.

Shipping:

The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.

Reconstitution:

Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Stability & Storage:

24 months, -20 to -70 °C, under powder state;

12 months, -20 to -70 °C, under sterile conditions after reconstitution;

2 month, 2 to 8 °C under sterile conditions after reconstitution;

avoid repeated freeze-thaw cycles.

                                                                                                                          

References:

1.          Finch, P.W. and J.S. Rubin, Keratinocyte growth factor expression and activity in cancer: implications for use in patients with solid tumors. J Natl Cancer Inst, 2006. 98(12)

2.          Niu, J., et al., Keratinocyte growth factor/fibroblast growth factor-7-regulated cell migration and invasion through activation of NF-kappaB transcription factors. J Biol Chem, 2007. 282(9)

3.          Qiao, J., et al., FGF-7 modulates ureteric bud growth and nephron number in the developing kidney. Development, 1999. 126(3)

4.          Guo, L., L. Degenstein, and E. Fuchs, Keratinocyte growth factor is required for hair development but not for wound healing. Genes Dev, 1996. 10(2):

OrganRegen-Recombinant Human EGF（568-EGF）

发表于由

Recombinant Human EGF

货号：568-EGF

规格：1mg

100ug

品牌：OrganRegen


产品介绍

DESCRIPTION

Background:

Epidermal growth factor (EGF) is a small, potent growth factor capable of inducing cell proliferation, differentiation, and survival. EGF is the founding member of the EGF family which are characterized by a shared structural motif, the EGF- like domain[1]. EGF is a high affinity ligand of the EGF receptor (ErbB). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members[2]. EGF binding induces dimerization of the EGF receptor resulting in activation of the protein tyrosine kinase signaling pathway. These receptors undergo a complex pattern of ligand-induced homo- or hetero-dimerization to transduce EGF family signals[3, 4]. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules[2]. Biological activities ascribed to EGF include epithelial development, angiogenesis, fibroblast proliferation, and colony formation of epidermal cells in culture.

技术参数

Source:

E.coli

Protein Construction:

A DNA sequence encoding the amino acids (Asn971-Arg1021) of human EGF (Accession Number: P01133) was expressed.

Synonyms:

hEGF; HOMG4; beta-urogastrone; epidermal growth factor; pro-epidermal growth factor; URG; Urogastrone

SPECIFICATIONS

Purity:

≥ 98%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.

Biological Activity:

Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.3-1.5 ng/mL.

Endotoxin Level:

<0.01 EU per 1 μg of the protein by the LAL method

Calculated Molecular Weight:

5.95 kDa

SDS-PAGE:

8 kDa, reducing conditions 

DATA

OrganRegen-Recombinant Human EGF（568-EGF） 

 FORMULATION AND STORAGE

Formulation:

The product is Lyophilized from a 0.22 μm filtered solution in PBS.

Shipping:

The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.

Reconstitution:

Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Stability & Storage:

24 months, -20 to -70 °C, under powder state;

12 months, -20 to -70 °C, under sterile conditions after reconstitution;

2 month, 2 to 8 °C under sterile conditions after reconstitution;

avoid repeated freeze-thaw cycles.

                                                                                                                          

References:

1.          Carpenter, G., et al., Epidermal growth factor. Biol Chem, 1990. 265(14): p. 7709-12.

2.          Jorissen, R.N., et al., Epidermal growth factor receptor: mechanisms of activation and signalling. Exp Cell Res, 2003. 284(1): p. 31-53.

3.          Gamett, D.C., et al., Secondary dimerization between members of the epidermal growth factor receptor family. J. Biol. Chem, 1997. 272: p. 12052.

4.          Qian, X., et al., Heterodimerization of epidermal growth factor receptor and wild-type or kinase-deficient Neu: a mechanism of interreceptor kinase activation and transphosphorylation. Proc Natl Acad Sci U S A, 1994. 91(4): p. 1500-4.