Recombinant Human R-spondin1
货号：861-RS1
规格：1mg
100ug
品牌：OrganRegen
产品介绍
Background:
R-Spondin1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing the furin-like and thrombospondin structural domains. Postnatally, R-Spondin1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate. R-Spondin1 regulates Wnt/β-catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt coreceptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization. Recombinant Human R-Spondin1 is a 27.3 kDa protein consisting of 233 amino acid residues. Due to glycosylation, RSpondin1 migrates at an apparent molecular weight of approximately 41.0 kDa by SDS-PAGE analysis under reducing conditions.
Source:
Chinese Hamster Ovary cell line
Protein Construction:
A DNA sequence encoding the amino acids (Ala31-Ser263) of human Rspo-1 (Accession Number: Q2MKA7) was expressed.
Synonyms:
Roof plate-specific spondin-1, RSPO1
Purity:
≥ 95%, by SDS-PAGE visualized with quantitative densitometry by Coomassie® Blue Staining.
Biological Activity:
Measured by its ability to induce activation of β-catenin response in a Top-flash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.5 μg/ml in the presence of 5 ng/ml recombinant mouse Wnt3a.
Endotoxin Level:
<0.10 EU per 1 μg of the protein by the LAL method
Calculated Molecular Weight:
27.3 kDa
SDS-PAGE:
41.0 kDa, reducing condition
Formulation:
The product is Lyophilized from a 0.22μm filtered solution in PBS.
Shipping:
The product is shipped on ice. Upon receipt, store it immediately as methods recommended below.
Reconstitution:
Reconstitute in sterile PBS buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Stability & Storage:
24 months, -20 to -70 °C, under powder state; 12 months, -20 to -70 °C, under sterile conditions after reconstitution; 2 month, 2 to 8 °C under sterile conditions after reconstitution; avoid repeated freeze-thaw cycles.
References:
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Kim, K.A., et al., R-Spondin proteins: a novel link to beta-catenin activation. Cell Cycle, 2006. 5(1)
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Kamata, T., et al., R-spondin, a novel gene with thrombospondin type 1 domain, was expressed in the dorsal neural tube and affected in Wnts mutants. Biochim Biophys Acta, 2004. 1676(1)
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Hao, H.X., et al., ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner. Nature, 2012. 485(7397)